6WZG: Human secretin receptor Gs complex

Human secretin receptor Gs complex. Determined by electron microscopy at 2.3 Å resolution. Released 12 Aug 2020.

Method: Electron microscopy
Resolution: 2.3 Å
Organisms: Homo sapiens, Lama glama
Chains: 6
Atoms: 8,939
Mol. weight: 161.09 kDa
Released: 12 Aug 2020

Explore 6WZG in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6WZG contains 37 α-helices and 48 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 10 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	13-39	27
β-strand	41-47	7	1
α-helix	53-60	8
β-strand	208-214	7	1
β-strand	217-223	7	1
α-helix	234-238	5
β-strand	243-249	7	1
β-strand	256	1	2
β-strand	262	1	2
α-helix	265-278	14
β-strand	286-292	7	1
α-helix	294-303	10
α-helix	308-310	3
α-helix	313-317	5
α-helix	326-327	2
α-helix	332-352	21
β-strand	359-363	5	1
α-helix	371-391	21

Chain B: 4 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	4-23	20
α-helix	30-34	5
α-helix	38-39	2
β-strand	47-51	5	3
β-strand	58-63	6	4
β-strand	69-74	6	4
β-strand	78-83	6	4
β-strand	89-94	6	4
β-strand	100-105	6	5
β-strand	111-116	6	5
β-strand	121-125	5	5
β-strand	134-139	6	5
β-strand	146-151	6	6
β-strand	156-161	6	6
β-strand	166-170	5	6
β-strand	175-180	6	6
α-helix	186	1
β-strand	187-192	6	7
β-strand	198-203	6	7
β-strand	207-212	6	7
β-strand	218-223	6	7
β-strand	229-234	6	8
β-strand	240-245	6	8
β-strand	250-254	5	8
β-strand	260-264	5	8
β-strand	273-278	6	9
β-strand	284-289	6	9
β-strand	294-298	5	9
β-strand	303-308	6	9
β-strand	315-320	6	3
β-strand	327-331	5	3
β-strand	336-339	4	3

Chain G: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	9-23	15
α-helix	30-43	14

Chain N: 3 helices, 12 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	10
β-strand	11	1	11
β-strand	18-25	8	10
α-helix	29-31	3
β-strand	34-39	6	12
β-strand	45-51	7	12
β-strand	58-60	3	12
α-helix	62-64	3
β-strand	68-73	6	10
β-strand	78-83	6	10
α-helix	88-90	3
β-strand	92-98	7	12
β-strand	118	1	12
β-strand	122-124	3	12
β-strand	125	1	11

Chain P: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	2-26	25

Chain R: 17 helices, 0 β-strands

Element	Residues	Length
α-helix	31-47	17
α-helix	91-94	4
α-helix	112-114	3
α-helix	133-166	34
α-helix	173-200	28
α-helix	212-245	34
α-helix	255-279	25
α-helix	292-325	34
α-helix	333-351	19
α-helix	355-358	4
α-helix	363-365	3
α-helix	366-370	5
α-helix	371-376	6
α-helix	377-379	3
α-helix	380-385	6
α-helix	386-390	5
α-helix	393-407	15

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Secretin	P	protein	27	Homo sapiens	P09683 (AlphaFold model)
Secretin receptor	R	protein	453	Homo sapiens	P47872 (AlphaFold model)
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	A	protein	394	Homo sapiens	P63092 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	340	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Homo sapiens	P59768
Nanobody35	N	protein	138	Lama glama

Sequence of entity 1 (P), FASTA

>6WZG_1 Secretin (chains P)
HSDGTFTSELSRLREGARLQRLLQGLV

Sequence of entity 2 (R), FASTA

>6WZG_2 Secretin receptor (chains R)
DYKDDDDLEVLFQGPAHSTGALPRLCDVLQVLWEEQDQCLQELSREQTGDLGTEQPVPGC
EGMWDNISCWPSSVPGRMVEVECPRFLRMLTSRNGSLFRNCTQDGWSETFPRPNLACGVN
VNDSSNEKRHSYLLKLKVMYTVGYSSSLVMLLVALGILCAFRRLHCTRNYIHMHLFVSFI
LRALSNFIKDAVLFSSDDVTYCDAHRAGCKLVMVLFQYCIMANYSWLLVEGLYLHTLLAI
SFFSERKYLQGFVAFGWGSPAIFVALWAIARHFLEDVGCWDINANASIWWIIRGPVILSI
LINFILFINILRILMRKLRTQETRGNEVSHYKRLARSTLLLIPLFGIHYIVFAFSPEDAM
EIQLFFELALGSFQGLVVAVLYCFLNGEVQLEVQKKWQQWHLREFPLHPVASFSNSTKAS
HLEQSQGTCRTSIIPAGLEVLFQGPHHHHHHHH

Sequence of entity 3 (A), FASTA

>6WZG_3 Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKNTIVKQM
RILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELA
NPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLD
KIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFND
VTAIIFVVASSSYNMVIREDNQTNRLQAALKLFDSIWNNKWLRDTSVILFLNKQDLLAEK
VLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCY
PHFTCSVDTENIRRVFNDCRDIIQRMHLRQYELL

Sequence of entity 4 (B), FASTA

>6WZG_4 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYA
MHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNI
CSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTF
TGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNA
FATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDAL
KADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 5 (G), FASTA

>6WZG_5 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 6 (N), FASTA

>6WZG_6 Nanobody35 (chains N)
QVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISY
TGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQ
GTQVTVSSHHHHHHEPEA

Primary citation

Structure and dynamics of the active Gs-coupled human secretin receptor. Dong, M., Deganutti, G., Piper, S.J. et al. Nat Commun (2020) 11:4137-4137. DOI 10.1038/s41467-020-17791-4 · PubMed

Other PDB entries of the same protein (UniProt P09683 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7D3S 2.9 Å, Human SECR in complex with an engineered Gs heterotrimer
6WI9 4.3 Å, Human secretin receptor Gs complex

Browse structure collections

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