6YJ6: TFIIIC subcomplex tauA

Structure of the TFIIIC subcomplex tauA. Determined by electron microscopy at 3.1 Å resolution. Released 16 Sept 2020.

Method: Electron microscopy
Resolution: 3.1 Å
Organism: Saccharomyces cerevisiae
Chains: 3
Atoms: 13,525
Mol. weight: 247.31 kDa
Released: 16 Sept 2020

Explore 6YJ6 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6YJ6 contains 88 α-helices and 41 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 49 helices, 1 β-strand

Element	Residues	Length	Sheet
α-helix	132-139	8
α-helix	148-157	10
α-helix	162-175	14
α-helix	178-189	12
α-helix	196-208	13
α-helix	212-225	14
α-helix	232-243	12
α-helix	246-258	13
α-helix	264-276	13
α-helix	280-303	24
α-helix	343-347	5
α-helix	353-355	3
α-helix	357-370	14
α-helix	374-387	14
α-helix	395-398	4
α-helix	408-412	5
α-helix	416-418	3
α-helix	432-444	13
α-helix	448-455	8
α-helix	456-459	4
α-helix	467-479	13
α-helix	483-490	8
α-helix	491-495	5
α-helix	502-514	13
α-helix	518-528	11
α-helix	537-548	12
α-helix	552-570	19
α-helix	613-636	24
α-helix	648-664	17
α-helix	665-667	3
α-helix	674-676	3
α-helix	677-683	7
α-helix	684-686	3
α-helix	692-700	9
α-helix	736-748	13
α-helix	752-762	11
α-helix	775-786	12
α-helix	792-805	14
α-helix	810-819	10
α-helix	824-830	7
α-helix	833-851	19
α-helix	877-886	10
α-helix	891-902	12
α-helix	909-923	15
α-helix	930-952	23
α-helix	957-971	15
α-helix	975-987	13
α-helix	995-1008	14
α-helix	1012-1021	10
β-strand	1024	1	1

Chain B: 29 helices, 25 β-strands

Element	Residues	Length	Sheet
β-strand	29-30	2	2
β-strand	35-41	7	2
β-strand	44	1	1
α-helix	49-58	10
α-helix	62-69	8
β-strand	75	1	3
β-strand	78	1	3
β-strand	81-83	3	4
β-strand	89	1	5
β-strand	95	1	5
β-strand	103-105	3	4
β-strand	106-107	2	2
α-helix	108	1
α-helix	111-113	3
β-strand	114-121	8	2
α-helix	127-129	3
α-helix	133-139	7
β-strand	147-159	13	2
α-helix	164-166	3
α-helix	174-179	6
α-helix	180-184	5
α-helix	188-195	8
α-helix	209-211	3
α-helix	227-230	4
β-strand	264-266	3	6
β-strand	271-272	2	7
α-helix	287-298	12
α-helix	307-309	3
α-helix	311-328	18
β-strand	331-332	2	7
α-helix	334-337	4
α-helix	349-352	4
β-strand	357-360	4	7
β-strand	366	1	7
β-strand	369-371	3	7
α-helix	376-378	3
α-helix	380-382	3
β-strand	387-388	2	8
β-strand	391-392	2	9
α-helix	402-405	4
α-helix	407-408	2
β-strand	439-440	2	8
α-helix	441-444	4
α-helix	448-454	7
β-strand	464	1	10
β-strand	468	1	10
α-helix	472-490	19
α-helix	498-505	8
β-strand	568-569	2	9
α-helix	572-573	2
α-helix	574-584	11
β-strand	587-589	3	6
α-helix	1009-1014	6
α-helix	1019-1025	7

Chain C: 10 helices, 15 β-strands

Element	Residues	Length	Sheet
β-strand	5-10	6	11
β-strand	14	1	12
β-strand	36	1	12
α-helix	38-51	14
β-strand	61-63	3	11
α-helix	67-80	14
β-strand	84-86	3	11
α-helix	88-90	3
α-helix	108-114	7
β-strand	119	1	11
α-helix	136-157	22
β-strand	163-168	6	11
α-helix	170-181	12
β-strand	204-209	6	11
α-helix	210	1
α-helix	231-233	3
β-strand	236-238	3	11
α-helix	257-259	3
β-strand	292-299	8	2
β-strand	316-320	5	2
β-strand	328-331	4	2
β-strand	334-340	7	2
β-strand	346-349	4	2
α-helix	351-353	3
β-strand	421-431	11	2

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Transcription factor tau 131 kDa subunit	A	protein	1029	Saccharomyces cerevisiae	P33339 (AlphaFold model)
Transcription factor tau 95 kDa subunit,Transcription factor tau 95 kDa subunit	B	protein	685	Saccharomyces cerevisiae	P32367 (AlphaFold model)
Transcription factor tau 55 kDa subunit	C	protein	435	Saccharomyces cerevisiae	Q12415 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>6YJ6_1 Transcription factor tau 131 kDa subunit (chains A)
MAAGKLKKEQQNQSAERESADTGKVNDEDEEHLYGNIDDYKHLIQDEEYDDEDVPHDLQL
SEDEYNSERDSSLLAEFSDYGEISEDDEEDFMNAIREASNFKVKKKKKNDKGKSYGRQRK
ERVLDPEVAQLLSQANEAFVRNDLQVAERLFNEVIKKDARNFAAYETLGDIYQLQGRLND
CCNSWFLAAHLNASDWEFWKIVAILSADLDHVRQAIYCFSRVISLNPMEWESIYRRSMLY
KKTGQLARALDGFQRLYMYNPYDANILRELAILYVDYDRIEDSIELYMKVFNANVERREA
ILAALENALDSSDEESAAEGEDADEKEPLEQDEDRQMFPDINWKKIDAKYKCIPFDWSSL
NILAELFLKLAVSEVDGIKTIKKCARWIQRRESQTFWDHVPDDSEFDNRRFKNSTFDSLL
AAEKEKSYNIPIDIRVRLGLLRLNTDNLVEALNHFQCLYDETFSDVADLYFEAATALTRA
EKYKEAIDFFTPLLSLEEWRTTDVFKPLARCYKEIESYETAKEFYELAIKSEPDDLDIRV
SLAEVYYRLNDPETFKHMLVDVVEMRKHQVDETLHRISNEKSSNDTSDISSKPLLEDSKF
RTFRKKKRTPYDAERERIERERRITAKVVDKYEKMKKFELNSGLNEAKQASIWINTVSEL
VDIFSSVKNFFMKSRSRKFVGILRRTKKFNTELDFQIERLSKLAEGDSVFEGPLMEERVT
LTSATELRGLSYEQWFELFMELSLVIAKYQSVEDGLSVVETAQEVNVFFQDPERVKMMKF
VKLAIVLQMDDEEELAENLRGLLNQFQFNRKVLQVFMYSLCRGPSSLNILSSTIQQKFFL
RQLKAFDSCRYNTEVNGQASITNKEVYNPNKKSSPYLYYIYAVLLYSSRGFLSALQYLTR
LEEDIPDDPMVNLLMGLSHIHRAMQRLTAQRHFQIFHGLRYLYRYHKIRKSLYTDLEKQE
ADYNLGRAFHLIGLVSIAIEYYNRVLENYDDGKLKKHAAYNSIIIYQQSGNVELADHLME
KYLSIRSGG

Sequence of entity 2 (B), FASTA

>6YJ6_2 Transcription factor tau 95 kDa subunit,Transcription factor tau 95 kDa subunit (chains B)
MHHHHHHENLYFQSMPVEEPLATLSSIPDSSADQAPPLIADEFTLDLPRIPSLELPLNVS
TKHSSIQKAIKMCGGIEKVKEAFKEHGPIESQHGLQLYLNDDTDSDGSKSYFNEHPVIGK
RVPFRDESVILKVTMPKGTLSKNNNSVKDSIKSLKDSNKLRVTPVSIVDNTIKFREMSDF
QIKLDNVPSAREFKSSFGSLEWNNFKSFVNSVPDNDSQPQENIGNLILDRSVKIPSTDFQ
LPPPPKLSMVGFPLLYKYKANPFAKKKKNGVTEVKGTYIKNYQLFVHDLSDKTVIPSQAH
EQVLYDFEVAKKTKVYPGTKSDSKFYESLEECLKILRELFARRPIWVKRHLDGIVPKKIH
HTMKIALALISYRFTMGPWRNTYIKFGIDPRSSVEYAQYQTEYFKIERKLLSSPIVKKNV
PKPPPLVFESDTPGGIDSRFKFDGKRIPWYLMLQIDLLIGEPNIAEVFHNVEYLDKANEL
TGWFKELDLVKIRRIVKYELGCMVQGNYEYNKYKLKYFKTMLFVKESMVPENKNSEEGMG
VNTNKDADGDINMDAGSQMSSNAIEEDKGIAAGDDFDDNGAITEEPDDAALENEEMDTDQ
NLKVPASIDDDVDDVDADEEEQESFDVKTASFQDIINKIAKLDPKTAETMKSELKGFVDE
VDLXXXXXXXXXXXXXXXXXXXXXX

Sequence of entity 3 (C), FASTA

>6YJ6_3 Transcription factor tau 55 kDa subunit (chains C)
MVVNTIYIARHGYRSNWLPEGPYPDPLTGIDSDVPLAEHGVQQAKELAHYLLSLDNQPEA
AFASPFYRCLETVQPIAKLLEIPVYLERGIGEWYRPDRKPVIPVPAGYEILSKFFPGVIS
QEWDSTLTPNEKGETEQEMYMRFKKFWPLFIERVEKEYPNVECILLVTHAASKIALGMSL
LGYDNPRMSLNENGDKIRSGSCSLDKYEILKKSYDTIDETDDQTSFTYIPFSDRKWVLTM
NGNTEFLSSGEEMNWNFDCVAEAGSDADIKKRQMTKKTSSPIPEADDQTEVETVYISVDI
PSGNYKERTEIAKSAILQYSGLETDAPLFRIGNRLYEGSWERLVGTELAFPNAAHVHKKT
AGLLSPTEENETTNAGQSKGSSTANDPNIQIQEEDVGLPDSTNTSRDHTGDKEEVQSEKI
YRIKERIVLSNVRPM

Primary citation

Structure of the TFIIIC subcomplex tau A provides insights into RNA polymerase III pre-initiation complex formation. Vorlander, M.K., Jungblut, A., Karius, K. et al. Nat Commun (2020) 11:4905-4905. DOI 10.1038/s41467-020-18707-y · PubMed

Other PDB entries of the same protein (UniProt P33339 (AlphaFold model), which also has an AlphaFold model), best resolution first:

5AIO 3.15 Å, Crystal structure of t131 N-terminal TPR array
5AEM 3.4 Å, Structure of t131 N-terminal TPR array
9GCK 3.7 Å, yeast TFIIIC TauA subcomplex bound to a tRNA gene
8FFZ 3.8 Å, TFIIIA-TFIIIC-Brf1-TBP complex bound to 5S rRNA gene

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