9GCK: Yeast TFIIIC TauA subcomplex

yeast TFIIIC TauA subcomplex bound to a tRNA gene. Determined by electron microscopy at 3.7 Å resolution. Released 27 Nov 2024.

Method
Electron microscopy
Resolution
3.7 Å
Organism
Saccharomyces cerevisiae
Chains
6
Atoms
12,298
Mol. weight
403.31 kDa
Released
27 Nov 2024

Explore 9GCK in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9GCK contains 69 α-helices and 38 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 24 helices, 14 β-strands

ElementResiduesLengthSheet
α-helix740-75718
α-helix762-7654
α-helix766-7683
α-helix774-80835
α-helix815-8195
α-helix823-83715
α-helix843-8442
β-strand84511
α-helix848-8547
β-strand858-86032
α-helix875-88410
β-strand888-89032
α-helix893-8942
α-helix898-91518
β-strand920-92233
α-helix923-9264
α-helix934-94613
β-strand950-95343
β-strand956-95943
α-helix962-9709
α-helix973-99018
β-strand997-99824
α-helix1004-101512
β-strand1019-102354
α-helix1042-10454
α-helix1047-10482
β-strand1049-105354
α-helix1067-10693
β-strand108015
β-strand108615
α-helix1088-110417
β-strand110816
α-helix1110-11167
α-helix1123-113513
β-strand1139-114136
α-helix1143-11453
β-strand1147-114936
α-helix11501
Chain B: 20 helices, 1 β-strand
ElementResiduesLengthSheet
α-helix733-74614
α-helix753-76412
α-helix766-7694
α-helix772-78615
α-helix793-80311
α-helix810-81910
α-helix824-8263
α-helix827-8315
α-helix833-85119
α-helix858-8614
α-helix875-88612
α-helix891-90212
α-helix909-92315
α-helix931-95222
α-helix955-9573
α-helix959-97113
α-helix975-98713
α-helix993-100816
β-strand101016
α-helix1012-10187
α-helix1019-10235
Chain C: 13 helices, 9 β-strands
ElementResiduesLengthSheet
α-helix23-242
β-strand2511
α-helix26-283
β-strand29-3022
β-strand35-4062
α-helix49-5810
α-helix61-7010
β-strand81-8332
β-strand8917
β-strand9517
β-strand103-10752
β-strand114-12292
α-helix125-1284
α-helix133-1386
β-strand147-162162
α-helix163-1664
α-helix174-1796
α-helix180-1845
α-helix188-1958
α-helix209-2124
α-helix227-2304
α-helix239-2402
Chain D: 12 helices, 14 β-strands
ElementResiduesLengthSheet
β-strand5-1068
β-strand1419
α-helix15-173
α-helix22-265
α-helix34-352
β-strand3619
α-helix371
α-helix38-5316
β-strand61-6338
α-helix67-8014
β-strand84-8638
α-helix88-903
α-helix108-1147
β-strand119-12028
α-helix136-15722
β-strand163-16868
α-helix170-18112
β-strand204-20968
β-strand236-24388
β-strand291-29992
β-strand316-32052
β-strand328-33142
β-strand334-349162
α-helix351-3533
α-helix417-4193
β-strand421-433132

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Transcription factor tau 138 kDa subunitAprotein1201Saccharomyces cerevisiaeP34111 (AlphaFold model)
Transcription factor tau 131 kDa subunitBprotein1029Saccharomyces cerevisiaeP33339 (AlphaFold model)
Transcription factor tau 95 kDa subunitCprotein606Saccharomyces cerevisiaeP32367 (AlphaFold model)
Transcription factor tau 55 kDa subunitDprotein435Saccharomyces cerevisiaeQ12415 (AlphaFold model)
DNA (45-mer)EDNA45Saccharomyces cerevisiae
DNA (45-mer)FDNA45Saccharomyces cerevisiae
Sequence of entity 1 (A), FASTA
>9GCK_1 Transcription factor tau 138 kDa subunit (chains A)
MVLTIYPDELVQIVSDKIASNKGKITLNQLWDISGKYFDLSDKKVKQFVLSCVILKKDIE
VYCDGAITTKNVTDIIGDANHSYSVGITEDSLWTLLTGYTKKESTIGNSAFELLLEVAKS
GEKGINTMDLAQVTGQDPRSVTGRIKKINHLLTSSQLIYKGHVVKQLKLKKFSHDGVDSN
PYINIRDHLATIVEVVKRSKNGIRQIIDLKRELKFDKEKRLSKAFIAAIAWLDEKEYLKK
VLVVSPKNPAIKIRCVKYVKDIPDSKGSPSFEYDSNSADEDSVSDSKAAFEDEDLVEGLD
NFNATDLLQNQGLVMEEKEDAVKNEVLLNRFYPLQNQTYDIADKSGLKGISTMDVVNRIT
GKEFQRAFTKSSEYYLESVDKQKENTGGYRLFRIYDFEGKKKFFRLFTAQNFQKLTNAED
EISVPKGFDELGKSRTDLKTLNEDNFVALNNTVRFTTDSDGQDIFFWHGELKIPPNSKKT
PNKNKRKRQVKNSTNASVAGNISNPKRIKLEQHVSTAQEPKSAEDSPSSNGGTVVKGKVV
NFGGFSARSLRSLQRQRAILKVMNTIGGVAYLREQFYESVSKYMGSTTTLDKKTVRGDVD
LMVESEKLGARTEPVSGRKIIFLPTVGEDAIQRYILKEKDSKKATFTDVIHDTEIYFFDQ
TEKNRFHRGKKSVERIRKFQNRQKNAKIKASDDAISKKSTSVNVSDGKIKRRDKKVSAGR
TTVVVENTKEDKTVYHAGTKDGVQALIRAVVVTKSIKNEIMWDKITKLFPNNSLDNLKKK
WTARRVRMGHSGWRAYVDKWKKMLVLAIKSEKISLRDVEELDLIKLLDIWTSFDEKEIKR
PLFLYKNYEENRKKFTLVRDDTLTHSGNDLAMSSMIQREISSLKKTYTRKISASTKDLSK
SQSDDYIRTVIRSILIESPSTTRNEIEALKNVGNESIDNVIMDMAKEKQIYLHGSKLECT
DTLPDILENRGNYKDFGVAFQYRCKVNELLEAGNAIVINQEPSDISSWVLIDLISGELLN
MDVIPMVRNVRPLTYTSRRFEIRTLTPPLIIYANSQTKLNTARKSAVKVPLGKPFSRLWV
NGSGSIRPNIWKQVVTMVVNEIIFHPGITLSRLQSRCREVLSLHEISEICKWLLERQVLI
TTDFDGYWVNHNWYSIYESTENLYFQSMSASAWSHPQFEKGGGSGGGSGGSAWSHPQFEK
S
Sequence of entity 2 (B), FASTA
>9GCK_2 Transcription factor tau 131 kDa subunit (chains B)
MAAGKLKKEQQNQSAERESADTGKVNDEDEEHLYGNIDDYKHLIQDEEYDDEDVPHDLQL
SEDEYNSERDSSLLAEFSDYGEISEDDEEDFMNAIREASNFKVKKKKKNDKGKSYGRQRK
ERVLDPEVAQLLSQANEAFVRNDLQVAERLFNEVIKKDARNFAAYETLGDIYQLQGRLND
CCNSWFLAAHLNASDWEFWKIVAILSADLDHVRQAIYCFSRVISLNPMEWESIYRRSMLY
KKTGQLARALDGFQRLYMYNPYDANILRELAILYVDYDRIEDSIELYMKVFNANVERREA
ILAALENALDSSDEESAAEGEDADEKEPLEQDEDRQMFPDINWKKIDAKYKCIPFDWSSL
NILAELFLKLAVSEVDGIKTIKKCARWIQRRESQTFWDHVPDDSEFDNRRFKNSTFDSLL
AAEKEKSYNIPIDIRVRLGLLRLNTDNLVEALNHFQCLYDETFSDVADLYFEAATALTRA
EKYKEAIDFFTPLLSLEEWRTTDVFKPLARCYKEIESYETAKEFYELAIKSEPDDLDIRV
SLAEVYYRLNDPETFKHMLVDVVEMRKHQVDETLHRISNEKSSNDTSDISSKPLLEDSKF
RTFRKKKRTPYDAERERIERERRITAKVVDKYEKMKKFELNSGLNEAKQASIWINTVSEL
VDIFSSVKNFFMKSRSRKFVGILRRTKKFNTELDFQIERLSKLAEGDSVFEGPLMEERVT
LTSATELRGLSYEQWFELFMELSLVIAKYQSVEDGLSVVETAQEVNVFFQDPERVKMMKF
VKLAIVLQMDDEEELAENLRGLLNQFQFNRKVLQVFMYSLCRGPSSLNILSSTIQQKFFL
RQLKAFDSCRYNTEVNGQASITNKEVYNPNKKSSPYLYYIYAVLLYSSRGFLSALQYLTR
LEEDIPDDPMVNLLMGLSHIHRAMQRLTAQRHFQIFHGLRYLYRYHKIRKSLYTDLEKQE
ADYNLGRAFHLIGLVSIAIEYYNRVLENYDDGKLKKHAAYNSIIIYQQSGNVELADHLME
KYLSIRSGG
Sequence of entity 3 (C), FASTA
>9GCK_3 Transcription factor tau 95 kDa subunit (chains C)
HHHHHHENLYFQSMPVEEPLATLSSIPDSSADQAPPLIADEFTLDLPRIPSLELPLNVST
KHSSIQKAIKMCGGIEKVKEAFKEHGPIESQHGLQLYLNDDTDSDGSKSYFNEHPVIGKR
VPFRDESVILKVTMPKGTLSKNNNSVKDSIKSLKDSNKLRVTPVSIVDNTIKFREMSDFQ
IKLDNVPSAREFKSSFGSLEWNNFKSFVNSVPDNDSQPQENIGNLILDRSVKIPSTDFQL
PPPPKLSMVGFPLLYKYKANPFAKKKKNGVTEVKGTYIKNYQLFVHDLSDKTVIPSQAHE
QVLYDFEVAKKTKVYPGTKSDSKFYESLEECLKILRELFARRPIWVKRHLDGIVPKKIHH
TMKIALALISYRFTMGPWRNTYIKFGIDPRSSVEYAQYQTEYFKIERKLLSSPIVKKNVP
KPPPLVFESDTPGGIDSRFKFDGKRIPWYLMLQIDLLIGEPNIAEVFHNVEYLDKANELT
GWFKELDLVKIRRIVKYELGCMVQGNYEYNKYKLKYFKTMLFVKESMVPENKNSEEGMGV
NTNKDADGDINMDAGSQMSSNAIEEDKGIAAGDDFDDNGAITEEPDDAALENEEMDTDQN
LKVPAS
Sequence of entity 4 (D), FASTA
>9GCK_4 Transcription factor tau 55 kDa subunit (chains D)
MVVNTIYIARHGYRSNWLPEGPYPDPLTGIDSDVPLAEHGVQQAKELAHYLLSLDNQPEA
AFASPFYRCLETVQPIAKLLEIPVYLERGIGEWYRPDRKPVIPVPAGYEILSKFFPGVIS
QEWDSTLTPNEKGETEQEMYMRFKKFWPLFIERVEKEYPNVECILLVTHAASKIALGMSL
LGYDNPRMSLNENGDKIRSGSCSLDKYEILKKSYDTIDETDDQTSFTYIPFSDRKWVLTM
NGNTEFLSSGEEMNWNFDCVAEAGSDADIKKRQMTKKTSSPIPEADDQTEVETVYISVDI
PSGNYKERTEIAKSAILQYSGLETDAPLFRIGNRLYEGSWERLVGTELAFPNAAHVHKKT
AGLLSPTEENETTNAGQSKGSSTANDPNIQIQEEDVGLPDSTNTSRDHTGDKEEVQSEKI
YRIKERIVLSNVRPM
Sequence of entity 5 (E), FASTA
>9GCK_5 DNA (45-MER) (chains E)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
Sequence of entity 6 (F), FASTA
>9GCK_6 DNA (45-MER) (chains F)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Primary citation

Structural and kinetic insights into tRNA promoter engagement by yeast general transcription factor TFIIIC. Seifert-Davila, W., Chaban, A., Baudin, F. et al. Nucleic Acids Res (2025) 53. DOI 10.1093/nar/gkae1174 · PubMed

Other PDB entries of the same protein (UniProt P34111 (AlphaFold model), which also has an AlphaFold model), best resolution first:

Browse structure collections

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