7AAV: Human pre-Bact-2 spliceosome core structure
Human pre-Bact-2 spliceosome core structure. Determined by electron microscopy at 4.2 Å resolution. Released 9 Dec 2020.
- Method
- Electron microscopy
- Resolution
- 4.2 Å
- Organisms
- Homo sapiens, synthetic construct
- Chains
- 17
- Atoms
- 25,867
- Mol. weight
- 1079.06 kDa
- Ligands
- GTP, MG, KGN
- Released
- 9 Dec 2020
Explore 7AAV in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
7AAV contains 151 α-helices and 137 β-strands across 13 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain 8: 1 helix, 1 β-strand
| Element | Residues | Length | Sheet |
|---|
| β-strand | 115 | 1 | 27 |
| α-helix | 122-130 | 9 | |
Chain A: 70 helices, 39 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 66-69 | 4 | |
| α-helix | 85-90 | 6 | |
| α-helix | 91-94 | 4 | |
| α-helix | 95-104 | 10 | |
| β-strand | 115-120 | 6 | 31 |
| β-strand | 127-128 | 2 | 31 |
| α-helix | 137-157 | 21 | |
| α-helix | 163-165 | 3 | |
| α-helix | 211-214 | 4 | |
| β-strand | 228-229 | 2 | 32 |
| α-helix | 232-242 | 11 | |
| α-helix | 262-268 | 7 | |
| α-helix | 280-282 | 3 | |
| α-helix | 310-315 | 6 | |
| α-helix | 317-320 | 4 | |
| α-helix | 327-331 | 5 | |
| α-helix | 370-371 | 2 | |
| α-helix | 372-376 | 5 | |
| α-helix | 377-379 | 3 | |
| α-helix | 399-405 | 7 | |
| α-helix | 409-411 | 3 | |
| β-strand | 415-416 | 2 | 32 |
| α-helix | 441-459 | 19 | |
| α-helix | 474-477 | 4 | |
| β-strand | 483-488 | 6 | 31 |
| α-helix | 489-511 | 23 | |
| β-strand | 516-518 | 3 | 33 |
| β-strand | 524-526 | 3 | 33 |
| α-helix | 535-538 | 4 | |
| α-helix | 544-566 | 23 | |
| α-helix | 573-587 | 15 | |
| α-helix | 598-616 | 19 | |
| α-helix | 633-651 | 19 | |
| α-helix | 679-693 | 15 | |
| α-helix | 707-721 | 15 | |
| α-helix | 733-759 | 27 | |
| α-helix | 771-796 | 26 | |
| α-helix | 803-820 | 18 | |
| α-helix | 822-829 | 8 | |
| α-helix | 835-850 | 16 | |
| α-helix | 858-871 | 14 | |
| α-helix | 874-886 | 13 | |
| β-strand | 895-898 | 4 | 34 |
| β-strand | 905 | 1 | 17 |
| β-strand | 907-910 | 4 | 34 |
| α-helix | 913-933 | 21 | |
| β-strand | 940 | 1 | 35 |
| α-helix | 949-960 | 12 | |
| β-strand | 973-980 | 8 | 35 |
| α-helix | 990-1000 | 11 | |
| α-helix | 1003-1012 | 10 | |
| β-strand | 1017-1018 | 2 | 34 |
| β-strand | 1023-1024 | 2 | 34 |
| α-helix | 1037-1052 | 16 | |
| α-helix | 1055-1062 | 8 | |
| α-helix | 1077-1081 | 5 | |
| β-strand | 1085-1090 | 6 | 35 |
| β-strand | 1094-1101 | 8 | 35 |
| α-helix | 1103-1116 | 14 | |
| α-helix | 1123-1125 | 3 | |
| α-helix | 1144-1160 | 17 | |
| β-strand | 1174-1178 | 5 | 35 |
| β-strand | 1185-1188 | 4 | 36 |
| β-strand | 1193-1198 | 6 | 36 |
| β-strand | 1213-1217 | 5 | 36 |
| α-helix | 1218 | 1 | |
| β-strand | 1224-1231 | 8 | 36 |
| α-helix | 1232-1233 | 2 | |
| α-helix | 1234-1249 | 16 | |
| α-helix | 1256-1273 | 18 | |
| α-helix | 1282-1303 | 22 | |
| α-helix | 1314-1317 | 4 | |
| β-strand | 1334 | 1 | 37 |
| α-helix | 1335-1336 | 2 | |
| α-helix | 1337-1339 | 3 | |
| β-strand | 1341-1342 | 2 | 38 |
| β-strand | 1345 | 1 | 39 |
| β-strand | 1350 | 1 | 39 |
| β-strand | 1353-1354 | 2 | 38 |
| β-strand | 1363 | 1 | 37 |
| α-helix | 1375-1398 | 24 | |
| α-helix | 1419-1421 | 3 | |
| α-helix | 1427-1430 | 4 | |
| α-helix | 1436-1446 | 11 | |
| α-helix | 1468-1478 | 11 | |
| α-helix | 1480-1486 | 7 | |
| α-helix | 1490-1493 | 4 | |
| α-helix | 1529-1537 | 9 | |
| α-helix | 1539-1542 | 4 | |
| β-strand | 1554 | 1 | 40 |
| β-strand | 1561 | 1 | 40 |
| α-helix | 1567-1576 | 10 | |
| α-helix | 1582-1599 | 18 | |
| β-strand | 1606-1608 | 3 | 41 |
| β-strand | 1631-1633 | 3 | 41 |
| α-helix | 1637 | 1 | |
| β-strand | 1638 | 1 | 42 |
| α-helix | 1639 | 1 | |
| β-strand | 1642-1644 | 3 | 43 |
| β-strand | 1656 | 1 | 42 |
| β-strand | 1659-1660 | 2 | 41 |
| β-strand | 1665-1667 | 3 | 44 |
| α-helix | 1671-1673 | 3 | |
| α-helix | 1676-1686 | 11 | |
| β-strand | 1704-1706 | 3 | 44 |
| β-strand | 1712-1713 | 2 | 44 |
| β-strand | 1715-1717 | 3 | 43 |
| α-helix | 1723-1734 | 12 | |
| α-helix | 1740-1751 | 12 | |
Chain G: 0 helices, 31 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 194-200 | 7 | 18 |
| β-strand | 210-212 | 3 | 19 |
| β-strand | 218-221 | 4 | 19 |
| β-strand | 227-232 | 6 | 19 |
| β-strand | 238-243 | 6 | 19 |
| β-strand | 249-254 | 6 | 20 |
| β-strand | 261-265 | 5 | 20 |
| β-strand | 269 | 1 | 20 |
| β-strand | 270 | 1 | 21 |
| β-strand | 272-274 | 3 | 20 |
| β-strand | 279-280 | 2 | 20 |
| β-strand | 284 | 1 | 21 |
| β-strand | 291-296 | 6 | 22 |
| β-strand | 302-307 | 6 | 22 |
| β-strand | 313-316 | 4 | 22 |
| β-strand | 321-322 | 2 | 22 |
| β-strand | 333-337 | 5 | 23 |
| β-strand | 344-349 | 6 | 23 |
| β-strand | 355-358 | 4 | 23 |
| β-strand | 364-367 | 4 | 23 |
| β-strand | 396 | 1 | 24 |
| β-strand | 408 | 1 | 24 |
| β-strand | 416-421 | 6 | 25 |
| β-strand | 426-431 | 6 | 25 |
| β-strand | 435-436 | 2 | 26 |
| β-strand | 437-440 | 4 | 25 |
| β-strand | 446 | 1 | 25 |
| β-strand | 450-451 | 2 | 26 |
| β-strand | 465-469 | 5 | 18 |
| β-strand | 477-481 | 5 | 18 |
| β-strand | 486-491 | 6 | 18 |
Chain I: 10 helices, 6 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 23-29 | 7 | |
| α-helix | 33-36 | 4 | |
| α-helix | 44-53 | 10 | |
| β-strand | 60-61 | 2 | 28 |
| β-strand | 66-67 | 2 | 28 |
| α-helix | 69-80 | 12 | |
| α-helix | 84-92 | 9 | |
| α-helix | 97-110 | 14 | |
| α-helix | 113-120 | 8 | |
| α-helix | 122-125 | 4 | |
| β-strand | 129-133 | 5 | 29 |
| β-strand | 139-143 | 5 | 29 |
| α-helix | 144-151 | 8 | |
| β-strand | 157 | 1 | 30 |
| β-strand | 160 | 1 | 30 |
| α-helix | 168-174 | 7 | |
Chain K: 1 helix, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 272-313 | 42 | |
Chain L: 7 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 13-21 | 9 | |
| α-helix | 22-26 | 5 | |
| α-helix | 31-38 | 8 | |
| α-helix | 43-49 | 7 | |
| α-helix | 65-77 | 13 | |
| α-helix | 82-89 | 8 | |
| α-helix | 96-107 | 12 | |
Chain N: 2 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 92-99 | 8 | |
| α-helix | 121-133 | 13 | |
Chain P: 9 helices, 4 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 33-45 | 13 | 45 |
| β-strand | 50-57 | 8 | 45 |
| α-helix | 94-97 | 4 | |
| α-helix | 146-150 | 5 | |
| α-helix | 163 | 1 | |
| β-strand | 164 | 1 | 46 |
| α-helix | 165 | 1 | |
| α-helix | 166-169 | 4 | |
| α-helix | 176-178 | 3 | |
| β-strand | 182 | 1 | 46 |
| α-helix | 192-194 | 3 | |
| α-helix | 198-202 | 5 | |
| α-helix | 208-215 | 8 | |
5 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| 116 kDa U5 small nuclear ribonucleoprotein component | r | protein | 972 | Homo sapiens | Q15029 (AlphaFold model) |
| Protein BUD31 homolog | Q | protein | 144 | Homo sapiens | P41223 (AlphaFold model) |
| Cell division cycle 5-like protein | L | protein | 802 | Homo sapiens | Q99459 (AlphaFold model) |
| Spliceosome-associated protein CWC15 homolog | R | protein | 229 | Homo sapiens | Q9P013 (AlphaFold model) |
| Microfibrillar-associated protein 1 | K | protein | 439 | Homo sapiens | P55081 |
| Pleiotropic regulator 1 | G | protein | 514 | Homo sapiens | O43660 |
| MINX M3 pre-mRNA | Z | RNA | 230 | synthetic construct | |
| Pre-mRNA-processing factor 17 | 8 | protein | 579 | Homo sapiens | O60508 |
| Pre-mRNA-splicing factor 38A | I | protein | 312 | Homo sapiens | Q8NAV1 |
| Pre-mRNA-processing-splicing factor 8 | A | protein | 2335 | Homo sapiens | Q6P2Q9 |
| Pre-mRNA-splicing factor RBM22 | P | protein | 420 | Homo sapiens | Q9NW64 |
| SNW domain-containing protein 1 | v | protein | 536 | Homo sapiens | Q13573 |
5 more molecules are not listed.
Sequence of entity 1 (r), FASTA
>7AAV_1 116 kDa U5 small nuclear ribonucleoprotein component (chains r)
MDTDLYDEFGNYIGPELDSDEDDDELGRETKDLDEMDDDDDDDDVGDHDDDHPGMEVVLH
EDKKYYPTAEEVYGPEVETIVQEEDTQPLTEPIIKPVKTKKFTLMEQTLPVTVYEMDFLA
DLMDNSELIRNVTLCGHLHHGKTCFVDCLIEQTHPEIRKRYDQDLCYTDILFTEQERGVG
IKSTPVTVVLPDTKGKSYLFNIMDTPGHVNFSDEVTAGLRISDGVVLFIDAAEGVMLNTE
RLIKHAVQERLAVTVCINKIDRLILELKLPPTDAYYKLRHIVDEVNGLISMYSTDENLIL
SPLLGNVCFSSSQYSICFTLGSFAKIYADTFGDINYQEFAKRLWGDIYFNPKTRKFTKKA
PTSSSQRSFVEFILEPLYKILAQVVGDVDTSLPRTLDELGIHLTKEELKLNIRPLLRLVC
KKFFGEFTGFVDMCVQHIPSPKVGAKPKIEHTYTGGVDSDLGEAMSDCDPDGPLMCHTTK
MYSTDDGVQFHAFGRVLSGTIHAGQPVKVLGENYTLEDEEDSQICTVGRLWISVARYHIE
VNRVPAGNWVLIEGVDQPIVKTATITEPRGNEEAQIFRPLKFNTTSVIKIAVEPVNPSEL
PKMLDGLRKVNKSYPSLTTKVEESGEHVILGTGELYLDCVMHDLRKMYSEIDIKVADPVV
TFCETVVETSSLKCFAETPNKKNKITMIAEPLEKGLAEDIENEVVQITWNRKKLGEFFQT
KYDWDLLAARSIWAFGPDATGPNILVDDTLPSEVDKALLGSVKDSIVQGFQWGTREGPLC
DELIRNVKFKILDAVVAQEPLHRGGGQIIPTARRVVYSAFLMATPRLMEPYYFVEVQAPA
DCVSAVYTVLARRRGHVTQDAPIPGSPLYTIKAFIPAIDSFGFETDLRTHTQGQAFSLSV
FHHWQIVPGDPLDKSIVIRPLEPQPAPHLAREFMIKTRRRKGLSEDVSISKFFDDPMLLE
LAKQDVVLNYPM
Sequence of entity 2 (Q), FASTA
>7AAV_2 Protein BUD31 homolog (chains Q)
MPKVKRSRKAPPDGWELIEPTLDELDQKMREAETEPHEGKRKVESLWPIFRIHHQKTRYI
FDLFYKRKAISRELYEYCIKEGYADKNLIAKWKKQGYENLCCLRCIQTRDTNFGTNCICR
VPKSKLEVGRIIECTHCGCRGCSG
Sequence of entity 3 (L), FASTA
>7AAV_3 Cell division cycle 5-like protein (chains L)
MPRIMIKGGVWRNTEDEILKAAVMKYGKNQWSRIASLLHRKSAKQCKARWYEWLDPSIKK
TEWSREEEEKLLHLAKLMPTQWRTIAPIIGRTAAQCLEHYEFLLDKAAQRDNEEETTDDP
RKLKPGEIDPNPETKPARPDPIDMDEDELEMLSEARARLANTQGKKAKRKAREKQLEEAR
RLAALQKRRELRAAGIEIQKKRKRKRGVDYNAEIPFEKKPALGFYDTSEENYQALDADFR
KLRQQDLDGELRSEKEGRDRKKDKQHLKRKKESDLPSAILQTSGVSEFTKKRSKLVLPAP
QISDAELQEVVKVGQASEIARQTAEESGITNSASSTLLSEYNVTNNSVALRTPRTPASQD
RILQEAQNLMALTNVDTPLKGGLNTPLHESDFSGVTPQRQVVQTPNTVLSTPFRTPSNGA
EGLTPRSGTTPKPVINSTPGRTPLRDKLNINPEDGMADYSDPSYVKQMERESREHLRLGL
LGLPAPKNDFEIVLPENAEKELEEREIDDTYIEDAADVDARKQAIRDAERVKEMKRMHKA
VQKDLPRPSEVNETILRPLNVEPPLTDLQKSEELIKKEMITMLHYDLLHHPYEPSGNKKG
KTVGFGTNNSEHITYLEHNPYEKFSKEELKKAQDVLVQEMEVVKQGMSHGELSSEAYNQV
WEECYSQVLYLPGQSRYTRANLASKKDRIESLEKRLEINRGHMTTEAKRAAKMEKKMKIL
LGGYQSRAMGLMKQLNDLWDQIEQAHLELRTFEELKKHEDSAIPRRLECLKEDVQRQQER
EKELQHRYADLLLEKETLKSKF
Sequence of entity 4 (R), FASTA
>7AAV_4 Spliceosome-associated protein CWC15 homolog (chains R)
MTTAARPTFEPARGGRGKGEGDLSQLSKQYSSRDLPSHTKIKYRQTTQDAPEEVRNRDFR
RELEERERAAAREKNRDRPTREHTTSSSVSKKPRLDQIPAANLDADDPLTDEEDEDFEEE
SDDDDTAALLAELEKIKKERAEEQARKEQEQKAEEERIRMENILSGNPLLNLTGPSQPQA
NFKVKRRWDDDVVFKNCAKGVDDQKKDKRFVNDTLRSEFHKKFMEKYIK
Sequence of entity 5 (K), FASTA
>7AAV_5 Microfibrillar-associated protein 1 (chains K)
MSVPSALMKQPPIQSTAGAVPVRNEKGEISMEKVKVKRYVSGKRPDYAPMESSDEEDEEF
QFIKKAKEQEAEPEEQEEDSSSDPRLRRLQNRISEDVEERLARHRKIVEPEVVGESDSEV
EGDAWRMEREDSSEEEEEEIDDEEIERRRGMMRQRAQERKNEEMEVMEVEDEGRSGEESE
SESEYEEYTDSEDEMEPRLKPVFIRKKDRVTVQEREAEALKQKELEQEAKRMAEERRKYT
LKIVEEETKKELEENKRSLAALDALNTDDENDEEEYEAWKVRELKRIKRDREDREALEKE
KAEIERMRNLTEEERRAELRANGKVITNKAVKGKYKFLQKYYHRGAFFMDEDEEVYKRDF
SAPTLEDHFNKTILPKVMQVKNFGRSGRTKYTHLVDQDTTSFDSAWGQESAQNTKFFKQK
AAGVRDVFERPSAKKRKTT
Sequence of entity 6 (G), FASTA
>7AAV_6 Pleiotropic regulator 1 (chains G)
MVEEVQKHSVHTLVFRSLKRTHDMFVADNGKPVPLDEESHKRKMAIKLRNEYGPVLHMPT
SKENLKEKGPQNATDSYVHKQYPANQGQEVEYFVAGTHPYPPGPGVALTADTKIQRMPSE
SAAQSLAVALPLQTKADANRTAPSGSEYRHPGASDRPQPTAMNSIVMETGNTKNSALMAK
KAPTMPKPQWHPPWKLYRVISGHLGWVRCIAVEPGNQWFVTGSADRTIKIWDLASGKLKL
SLTGHISTVRGVIVSTRSPYLFSCGEDKQVKCWDLEYNKVIRHYHGHLSAVYGLDLHPTI
DVLVTCSRDSTARIWDVRTKASVHTLSGHTNAVATVRCQAAEPQIITGSHDTTIRLWDLV
AGKTRVTLTNHKKSVRAVVLHPRHYTFASGSPDNIKQWKFPDGSFIQNLSGHNAIINTLT
VNSDGVLVSGADNGTMHLWDWRTGYNFQRVHAAVQPGSLDSESGIFACAFDQSESRLLTA
EADKTIKVYREDDTATEETHPVSWKPEIIKRKRF
Sequence of entity 7 (Z), FASTA
>7AAV_7 MINX M3 pre-mRNA (chains Z)
GGGAGACGGAAUUCGAGCUCGCCCACUCUUGGAUCGGAAACCCGUCGGCCUCCGAACGGU
AAGAGCCUAGCAUGUAGAACUGGUUACCUGCAGCCCAAGCUUGCUGCACGUCUAGGGCGC
AGUAGUCCAGGGUUUCCUUGAUGAUGUCAUACUUAUCCUGUCCCUUUUUUUUCCACAGCU
CGCGGUUGAGGACAAACUCUUCGCGGUCUUUCCAGUGGGGAUCCAAUAUC
Sequence of entity 8 (8), FASTA
>7AAV_8 Pre-mRNA-processing factor 17 (chains 8)
MSAAIAALAASYGSGSGSESDSDSESSRCPLPAADSLMHLTKSPSSKPSLAVAVDSAPEV
AVKEDLETGVHLDPAVKEVQYNPTYETMFAPEFGPENPFRTQQMAAPRNMLSGYAEPAHI
NDFMFEQQRRTFATYGYALDPSLDNHQVSAKYIGSVEEAEKNQGLTVFETGQKKTEKRKK
FKENDASNIDGFLGPWAKYVDEKDVAKPSEEEQKELDEITAKRQKKGKQEEEKPGEEKTI
LHVKEMYDYQGRSYLHIPQDVGVNLRSTMPPEKCYLPKKQIHVWSGHTKGVSAVRLFPLS
GHLLLSCSMDCKIKLWEVYGERRCLRTFIGHSKAVRDICFNTAGTQFLSAAYDRYLKLWD
TETGQCISRFTNRKVPYCVKFNPDEDKQNLFVAGMSDKKIVQWDIRSGEIVQEYDRHLGA
VNTIVFVDENRRFVSTSDDKSLRVWEWDIPVDFKYIAEPSMHSMPAVTLSPNGKWLACQS
MDNQILIFGAQNRFRLNKKKIFKGHMVAGYACQVDFSPDMSYVISGDGNGKLNIWDWKTT
KLYSRFKAHDKVCIGAVWHPHETSKVITCGWDGLIKLWD
Sequence of entity 9 (I), FASTA
>7AAV_9 Pre-mRNA-splicing factor 38A (chains I)
MANRTVKDAHSIHGTNPQYLVEKIIRTRIYESKYWKEECFGLTAELVVDKAMELRFVGGV
YGGNIKPTPFLCLTLKMLQIQPEKDIIVEFIKNEDFKYVRMLGALYMRLTGTAIDCYKYL
EPLYNDYRKIKSQNRNGEFELMHVDEFIDELLHSERVCDIILPRLQKRYVLEEAEQLEPR
VSALEEDMDDVESSEEEEEEDEKLERVPSPDHRRRSYRDLDKPRRSPTLRYRRSRSRSPR
RRSRSPKRRSPSPRRERHRSKSPRRHRSRSRDRRHRSRSKSPGHHRSHRHRSHSKSPERS
KKSHKKSRRGNE
Sequence of entity 10 (A), FASTA
>7AAV_10 Pre-mRNA-processing-splicing factor 8 (chains A)
MAGVFPYRGPGNPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRYAEKRKFGFVDAQKED
MPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLY
HITGAISFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYAD
NILDVEPLEAIQLELDPEEDAPVLDWFYDHQPLRDSRKYVNGSTYQRWQFTLPMMSTLYR
LANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDIN
KIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPIS
HRHSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRR
ALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATK
FFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRF
GNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMR
QIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQF
EGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCW
KANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLT
RLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLI
LALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMD
LYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGIN
NLQDVWETSEGECNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYK
DMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDIATE
AAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARM
RLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKC
RTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIV
NKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPK
ELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFI
DSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRT
DFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEG
LFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGI
FMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSY
KMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYA
RAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPAL
YVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTT
KPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQ
IIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLY
DDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTITEPHHIWPTLTDEEWIKVE
VQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLT
ATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKET
GYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLP
GQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTP
GSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWN
YNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA
Sequence of entity 11 (P), FASTA
>7AAV_11 Pre-mRNA-splicing factor RBM22 (chains P)
MATSLGSNTYNRQNWEDADFPILCQTCLGENPYIRMTKEKYGKECKICARPFTVFRWCPG
VRMRFKKTEVCQTCSKLKNVCQTCLLDLEYGLPIQVRDAGLSFKDDMPKSDVNKEYYTQN
MEREISNSDGTRPVGMLGKATSTSDMLLKLARTTPYYKRNRPHICSFWVKGECKRGEECP
YRHEKPTDPDDPLADQNIKDRYYGINDPVADKLLKRASTMPRLDPPEDKTITTLYVGGLG
DTITETDLRNHFYQFGEIRTITVVQRQQCAFIQFATRQAAEVAAEKSFNKLIVNGRRLNV
KWGRSQAARGKEKEKDGTTDSGIKLEPVPGLPGALPPPPAAEEEASANYFNLPPSGPPAV
VNIALPPPPGIAPPPPPGFGPHMFHPMGPPPPFMRAPGPIHYPSQDPQRMGAHAGKHSSP
Sequence of entity 12 (v), FASTA
>7AAV_12 SNW domain-containing protein 1 (chains v)
MALTSFLPAPTQLSQDQLEAEEKARSQRSRQTSLVSSRREPPPYGYRKGWIPRLLEDFGD
GGAFPEIHVAQYPLDMGRKKKMSNALAIQVDSEGKIKYDAIARQGQSKDKVIYSKYTDLV
PKEVMNADDPDLQRPDEEAIKEITEKTRVALEKSVSQKVAAAMPVRAADKLAPAQYIRYT
PSQQGVAFNSGAKQRVIRMVEMQKDPMEPPRFKINKKIPRGPPSPPAPVMHSPSRKMTVK
EQQEWKIPPCISNWKNAKGYTIPLDKRLAADGRGLQTVHINENFAKLAEALYIADRKARE
AVEMRAQVERKMAQKEKEKHEEKLREMAQKARERRAGIKTHVEKEDGEARERDEIRHDRR
KERQHDRNLSRAAPDKRSKLQRNENRDISEVIALGVPNPRTSNEVQYDQRLFNQSKGMDS
GFAGGEDEIYNVYDQAWRGGKDMAQSIYRPSKNLDKDMYGDDLEARIKTNRFVPDKEFSG
SDRRQRGREGPVQFEEDPFGLDKFLEEAKQHGGSKRPSDSSRPKEHEHEGKKRRKE
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| GTP | Guanosine-5'-triphosphate | C10 H16 N5 O14 P3 | 1 |
| MG | Magnesium ion | Mg | 1 |
| KGN | D-chiro inositol hexakisphosphate | C6 H18 O24 P6 | 1 |
Primary citation
Mechanism of protein-guided folding of the active site U2/U6 RNA during spliceosome activation. Townsend, C., Leelaram, M.N., Agafonov, D.E. et al. Science (2020) 370. DOI 10.1126/science.abc3753 · PubMed
Other PDB entries of the same protein (UniProt Q15029 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 9S2E 2.4 Å, Human intron-lariat spliceosome-Aquarius (ILSaqr)
- 8H6L 2.6 Å, Cryo-EM structure of human exon-defined spliceosome in the early B state.
- 8H6K 2.7 Å, Cryo-EM structure of human exon-defined spliceosome in the mature B state.
- 8C6J 2.8 Å, Human spliceosomal PM5 C* complex
- 6ID1 2.86 Å, Cryo-EM structure of a human intron lariat spliceosome after Prp43 loaded (ILS2 complex)…
- 7DVQ 2.89 Å, Cryo-EM Structure of the Activated Human Minor Spliceosome (minor Bact Complex)
- 6ID0 2.9 Å, Cryo-EM structure of a human intron lariat spliceosome prior to Prp43 loaded (ILS1…
- 6QW6 2.92 Å, Structure of the human U5.U4/U6 tri-snRNP at 2.9A resolution.
- 9XTT 2.92 Å, Human minor spliceosome branching-completed C complex (after step-I)
- 6ICZ 3.0 Å, Cryo-EM structure of a human post-catalytic spliceosome (P complex) at 3.0 angstrom
- 8I0R 3.0 Å, The cryo-EM structure of human Bact-I complex
- 8I0T 3.0 Å, The cryo-EM structure of human Bact-III complex
Browse structure collections
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