9S2E: Human intron-lariat spliceosome-Aquarius
Human intron-lariat spliceosome-Aquarius (ILSaqr). Determined by electron microscopy at 2.4 Å resolution. Released 29 Jul 2026.
- Method
- Electron microscopy
- Resolution
- 2.4 Å
- Organism
- Homo sapiens
- Chains
- 42
- Atoms
- 97,335
- Mol. weight
- 2591.39 kDa
- Ligands
- ZN, GTP, IHP, MG
- Released
- 29 Jul 2026
Explore 9S2E in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
9S2E contains 669 α-helices and 413 β-strands across 38 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain 3: 8 helices, 3 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 36-38 | 3 | 6 |
| α-helix | 40-55 | 16 | |
| α-helix | 58-74 | 17 | |
| α-helix | 77-86 | 10 | |
| α-helix | 148-154 | 7 | |
| β-strand | 155-157 | 3 | 6 |
| α-helix | 158-177 | 20 | |
| α-helix | 180-186 | 7 | |
| α-helix | 227-232 | 6 | |
| β-strand | 243-244 | 2 | 7 |
| α-helix | 246-249 | 4 | |
Chain a: 3 helices, 7 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 5-12 | 8 | |
| β-strand | 17-22 | 6 | 31 |
| β-strand | 27-35 | 9 | 31 |
| β-strand | 41-43 | 3 | 31 |
| β-strand | 46-49 | 4 | 31 |
| β-strand | 55-58 | 4 | 31 |
| β-strand | 61-63 | 3 | 31 |
| α-helix | 65-67 | 3 | |
| β-strand | 68-73 | 6 | 31 |
| α-helix | 75-78 | 4 | |
Chain A: 114 helices, 52 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 27-44 | 18 | |
| α-helix | 47-49 | 3 | |
| α-helix | 53-55 | 3 | |
| α-helix | 59-62 | 4 | |
| α-helix | 63-73 | 11 | |
| α-helix | 80-84 | 5 | |
| α-helix | 85-90 | 6 | |
| α-helix | 91-94 | 4 | |
| α-helix | 95-104 | 10 | |
| α-helix | 106-108 | 3 | |
| β-strand | 115-121 | 7 | 14 |
| β-strand | 127-129 | 3 | 14 |
| β-strand | 134-135 | 2 | 15 |
| α-helix | 137-157 | 21 | |
| α-helix | 162-165 | 4 | |
| α-helix | 173-177 | 5 | |
| α-helix | 178-181 | 4 | |
| α-helix | 186-190 | 5 | |
| β-strand | 220 | 1 | 16 |
| β-strand | 227-229 | 3 | 16 |
| α-helix | 232-241 | 10 | |
| α-helix | 242-244 | 3 | |
| α-helix | 252-255 | 4 | |
| α-helix | 260-268 | 9 | |
| α-helix | 299-301 | 3 | |
| α-helix | 310-315 | 6 | |
| α-helix | 329-331 | 3 | |
| α-helix | 335-337 | 3 | |
| α-helix | 357-359 | 3 | |
| α-helix | 371-373 | 3 | |
| α-helix | 396-404 | 9 | |
| β-strand | 415-417 | 3 | 16 |
| β-strand | 419-420 | 2 | 15 |
| α-helix | 428-432 | 5 | |
| α-helix | 434-436 | 3 | |
| α-helix | 441-459 | 19 | |
| α-helix | 463-465 | 3 | |
| α-helix | 472-477 | 6 | |
| β-strand | 482-488 | 7 | 14 |
| α-helix | 489-510 | 22 | |
| β-strand | 516-518 | 3 | 17 |
| β-strand | 524-526 | 3 | 17 |
| α-helix | 529-531 | 3 | |
| α-helix | 532-537 | 6 | |
| α-helix | 542-565 | 24 | |
| α-helix | 571-583 | 13 | |
| α-helix | 585-589 | 5 | |
| α-helix | 591-593 | 3 | |
| α-helix | 596-598 | 3 | |
| α-helix | 599-616 | 18 | |
| α-helix | 631-661 | 31 | |
| α-helix | 669-671 | 3 | |
| α-helix | 677-696 | 20 | |
| α-helix | 706-721 | 16 | |
| α-helix | 724-726 | 3 | |
| α-helix | 733-763 | 31 | |
| α-helix | 769-796 | 28 | |
| α-helix | 803-820 | 18 | |
| α-helix | 822-824 | 3 | |
| β-strand | 825 | 1 | 18 |
| α-helix | 826-827 | 2 | |
| α-helix | 835-847 | 13 | |
| α-helix | 848-850 | 3 | |
| α-helix | 858-872 | 15 | |
| α-helix | 874-887 | 14 | |
| α-helix | 892-893 | 2 | |
| β-strand | 894-900 | 7 | 19 |
| β-strand | 905-910 | 6 | 19 |
| α-helix | 913-932 | 20 | |
| β-strand | 940 | 1 | 20 |
| α-helix | 948-960 | 13 | |
| β-strand | 973-980 | 8 | 20 |
| α-helix | 985-987 | 3 | |
| α-helix | 990-1000 | 11 | |
| β-strand | 1001 | 1 | 18 |
| α-helix | 1003-1011 | 9 | |
| β-strand | 1016-1019 | 4 | 19 |
| β-strand | 1022-1025 | 4 | 19 |
| α-helix | 1037-1053 | 17 | |
| α-helix | 1055-1062 | 8 | |
| α-helix | 1077-1080 | 4 | |
| β-strand | 1085-1091 | 7 | 20 |
| β-strand | 1094-1101 | 8 | 20 |
| α-helix | 1103-1116 | 14 | |
| α-helix | 1123-1125 | 3 | |
| α-helix | 1140-1141 | 2 | |
| α-helix | 1144-1158 | 15 | |
| β-strand | 1174-1178 | 5 | 20 |
| β-strand | 1179 | 1 | 21 |
| β-strand | 1182 | 1 | 21 |
| β-strand | 1185-1189 | 5 | 22 |
| β-strand | 1192-1198 | 7 | 22 |
| β-strand | 1213-1217 | 5 | 22 |
| β-strand | 1224-1232 | 9 | 22 |
| α-helix | 1234-1250 | 17 | |
| α-helix | 1256-1274 | 19 | |
| α-helix | 1275-1277 | 3 | |
| α-helix | 1282-1302 | 21 | |
| α-helix | 1314-1317 | 4 | |
| α-helix | 1320-1322 | 3 | |
| β-strand | 1332 | 1 | 23 |
| β-strand | 1334 | 1 | 24 |
| α-helix | 1337-1340 | 4 | |
| β-strand | 1341-1346 | 6 | 25 |
| β-strand | 1349-1354 | 6 | 25 |
| α-helix | 1363 | 1 | |
| β-strand | 1364 | 1 | 24 |
| α-helix | 1365-1367 | 3 | |
| α-helix | 1368-1371 | 4 | |
| α-helix | 1375-1399 | 25 | |
| α-helix | 1402-1404 | 3 | |
| α-helix | 1405-1408 | 4 | |
| α-helix | 1419-1423 | 5 | |
| α-helix | 1427-1430 | 4 | |
| α-helix | 1436-1442 | 7 | |
| α-helix | 1443-1445 | 3 | |
| α-helix | 1468-1477 | 10 | |
| α-helix | 1480-1485 | 6 | |
| α-helix | 1491-1493 | 3 | |
| β-strand | 1501-1503 | 3 | 26 |
| α-helix | 1507-1514 | 8 | |
| α-helix | 1520-1537 | 18 | |
| α-helix | 1539-1542 | 4 | |
| β-strand | 1552-1554 | 3 | 27 |
| α-helix | 1555 | 1 | |
| β-strand | 1561-1563 | 3 | 27 |
| α-helix | 1569-1577 | 9 | |
| α-helix | 1581-1599 | 19 | |
| β-strand | 1606-1611 | 6 | 28 |
| α-helix | 1616-1618 | 3 | |
| β-strand | 1629-1638 | 10 | 28 |
| α-helix | 1639-1642 | 4 | |
| β-strand | 1643-1644 | 2 | 28 |
| β-strand | 1656-1667 | 12 | 28 |
| α-helix | 1676-1689 | 14 | |
| β-strand | 1700-1706 | 7 | 28 |
| β-strand | 1711-1716 | 6 | 28 |
| α-helix | 1722-1736 | 15 | |
| α-helix | 1738-1751 | 14 | |
| β-strand | 1753-1755 | 3 | 26 |
| α-helix | 1761-1762 | 2 | |
| β-strand | 1763 | 1 | 29 |
| α-helix | 1765-1772 | 8 | |
| β-strand | 1777-1781 | 5 | 2 |
| β-strand | 1787-1792 | 6 | 30 |
| β-strand | 1798-1803 | 6 | 30 |
| β-strand | 1805-1810 | 6 | 2 |
| β-strand | 1816-1822 | 7 | 2 |
| α-helix | 1824-1827 | 4 | |
| α-helix | 1837-1851 | 15 | |
| α-helix | 1854-1856 | 3 | |
| β-strand | 1860-1863 | 4 | 2 |
| α-helix | 1866-1868 | 3 | |
| α-helix | 1869-1875 | 7 | |
| α-helix | 1882 | 1 | |
| β-strand | 1883-1886 | 4 | 2 |
| β-strand | 1887 | 1 | 29 |
| α-helix | 1893-1898 | 6 | |
| α-helix | 1900-1908 | 9 | |
| β-strand | 1913-1918 | 6 | 2 |
| α-helix | 1923-1925 | 3 | |
| α-helix | 1929-1946 | 18 | |
| α-helix | 1948-1954 | 7 | |
| α-helix | 1973-1995 | 23 | |
| α-helix | 1999-2001 | 3 | |
| α-helix | 2004-2012 | 9 | |
Chain b: 5 helices, 7 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 7-11 | 5 | |
| β-strand | 16-21 | 6 | 31 |
| β-strand | 26-33 | 8 | 31 |
| β-strand | 40-50 | 11 | 31 |
| β-strand | 62-67 | 6 | 31 |
| β-strand | 69-72 | 4 | 31 |
| α-helix | 74-76 | 3 | |
| β-strand | 77-84 | 8 | 31 |
| α-helix | 85-87 | 3 | |
| α-helix | 100-102 | 3 | |
| β-strand | 106-109 | 4 | 47 |
| α-helix | 114-116 | 3 | |
Chain B: 0 helices, 1 β-strand
| Element | Residues | Length | Sheet |
|---|
| β-strand | 41 | 1 | 23 |
Chain c: 3 helices, 5 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 4-8 | 5 | |
| β-strand | 14-19 | 6 | 31 |
| β-strand | 24-32 | 9 | 31 |
| β-strand | 38-46 | 9 | 31 |
| β-strand | 53-60 | 8 | 31 |
| α-helix | 62-64 | 3 | |
| β-strand | 65-70 | 6 | 31 |
| α-helix | 76-79 | 4 | |
Chain C: 54 helices, 41 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 60-62 | 3 | |
| α-helix | 69-73 | 5 | |
| β-strand | 78-81 | 4 | 32 |
| β-strand | 103 | 1 | 33 |
| α-helix | 109-111 | 3 | |
| α-helix | 116-122 | 7 | |
| α-helix | 126-128 | 3 | |
| β-strand | 129-136 | 8 | 34 |
| α-helix | 142-153 | 12 | |
| β-strand | 171 | 1 | 33 |
| α-helix | 172-177 | 6 | |
| β-strand | 184-191 | 8 | 34 |
| β-strand | 197-204 | 8 | 34 |
| α-helix | 209-211 | 3 | |
| α-helix | 212-221 | 10 | |
| β-strand | 224-230 | 7 | 34 |
| α-helix | 237-248 | 12 | |
| α-helix | 251 | 1 | |
| β-strand | 252-258 | 7 | 34 |
| α-helix | 261 | 1 | |
| α-helix | 262-266 | 5 | |
| α-helix | 271-292 | 22 | |
| β-strand | 307-311 | 5 | 34 |
| β-strand | 316-318 | 3 | 34 |
| α-helix | 320-331 | 12 | |
| α-helix | 336-339 | 4 | |
| α-helix | 340-342 | 3 | |
| β-strand | 348-350 | 3 | 35 |
| β-strand | 355-357 | 3 | 35 |
| α-helix | 365-366 | 2 | |
| β-strand | 367 | 1 | 35 |
| α-helix | 368-369 | 2 | |
| α-helix | 370-374 | 5 | |
| α-helix | 375-386 | 12 | |
| α-helix | 392-399 | 8 | |
| α-helix | 407-409 | 3 | |
| α-helix | 412-424 | 13 | |
| α-helix | 428-437 | 10 | |
| α-helix | 441-452 | 12 | |
| β-strand | 453 | 1 | 36 |
| α-helix | 460-466 | 7 | |
| β-strand | 475-483 | 9 | 37 |
| β-strand | 490-498 | 9 | 37 |
| β-strand | 501-502 | 2 | 38 |
| β-strand | 506-510 | 5 | 37 |
| β-strand | 522-526 | 5 | 37 |
| β-strand | 530-533 | 4 | 37 |
| β-strand | 538-541 | 4 | 37 |
| β-strand | 543-544 | 2 | 38 |
| β-strand | 549-552 | 4 | 37 |
| β-strand | 563-566 | 4 | 37 |
| α-helix | 576 | 1 | |
| β-strand | 577 | 1 | 36 |
| α-helix | 578-580 | 3 | |
| β-strand | 588-594 | 7 | 39 |
| α-helix | 597-599 | 3 | |
| α-helix | 600-613 | 14 | |
| β-strand | 618-621 | 4 | 39 |
| β-strand | 627-631 | 5 | 39 |
| α-helix | 634-643 | 10 | |
| α-helix | 644-649 | 6 | |
| β-strand | 653-655 | 3 | 39 |
| α-helix | 656-658 | 3 | |
| β-strand | 659-660 | 2 | 39 |
| β-strand | 663-666 | 4 | 40 |
| β-strand | 674-677 | 4 | 41 |
| β-strand | 684-691 | 8 | 41 |
| α-helix | 692-693 | 2 | |
| α-helix | 696-701 | 6 | |
| α-helix | 711-722 | 12 | |
| α-helix | 726-730 | 5 | |
| β-strand | 732-736 | 5 | 41 |
| β-strand | 743-746 | 4 | 41 |
| α-helix | 756-760 | 5 | |
| α-helix | 763-776 | 14 | |
| β-strand | 784 | 1 | 40 |
| β-strand | 787-795 | 9 | 41 |
| α-helix | 800-803 | 4 | |
| α-helix | 805-821 | 17 | |
| β-strand | 825-839 | 15 | 40 |
| α-helix | 840-842 | 3 | |
| α-helix | 843-852 | 10 | |
| β-strand | 856-862 | 7 | 40 |
| α-helix | 863 | 1 | |
| β-strand | 868-876 | 9 | 40 |
| α-helix | 877-879 | 3 | |
| α-helix | 883-890 | 8 | |
| β-strand | 896-906 | 11 | 40 |
| α-helix | 907 | 1 | |
| α-helix | 916-919 | 4 | |
| α-helix | 923-925 | 3 | |
| α-helix | 926-928 | 3 | |
| α-helix | 929-941 | 13 | |
| α-helix | 949-951 | 3 | |
| α-helix | 955-962 | 8 | |
Chain d: 4 helices, 5 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 18-26 | 9 | |
| α-helix | 30-38 | 9 | |
| β-strand | 41-46 | 6 | 31 |
| β-strand | 51-59 | 9 | 31 |
| β-strand | 65-76 | 12 | 31 |
| α-helix | 84-87 | 4 | |
| β-strand | 90-101 | 12 | 31 |
| α-helix | 103-105 | 3 | |
| β-strand | 106-111 | 6 | 31 |
30 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| U2 snRNA | 2 | RNA | 187 | Homo sapiens | |
| Splicing factor ESS-2 homolog | 3 | protein | 476 | Homo sapiens | Q96DF8 (AlphaFold model) |
| U5 snRNA | 5 | RNA | 116 | Homo sapiens | |
| U6 snRNA | 6 | RNA | 106 | Homo sapiens | |
| Pre-mRNA-processing-splicing factor 8 | A | protein | 2335 | Homo sapiens | Q6P2Q9 (AlphaFold model) |
| U5 small nuclear ribonucleoprotein 200 kDa helicase | B | protein | 2136 | Homo sapiens | O75643 (AlphaFold model) |
| 116 kDa U5 small nuclear ribonucleoprotein component | C | protein | 972 | Homo sapiens | Q15029 (AlphaFold model) |
| Pre-mRNA-splicing factor ISY1 homolog | D | protein | 285 | Homo sapiens | Q9ULR0 |
| ATP-dependent RNA helicase DHX15 | DX | protein | 795 | Homo sapiens | O43143 |
| U5 small nuclear ribonucleoprotein 40 kDa protein | E | protein | 357 | Homo sapiens | Q96DI7 |
| Intron Large complex component GCFC2 | GC | protein | 781 | Homo sapiens | P16383 |
| Pre-mRNA-splicing factor SYF1 | I | protein | 855 | Homo sapiens | Q9HCS7 |
27 more molecules are not listed.
Sequence of entity 1 (2), FASTA
>9S2E_1 U2 snRNA (chains 2)
AUCGCUUCUCGGCCUUUUGGCUAAGAUCAAGUGUAGUAUCUGUUCUUAUCAGUUUAAUAU
CUGAUACGUCCUCUAUCCGAGGACAAUAUAUUAAAAGAUUUUUGGAACUGGGAGAUGGAA
UAGGAGCUUGCUCCGUCCACUCCACGCAUCGACCUGGUAUUGCAGUACCUCCAGGAACGG
UGCACCC
Sequence of entity 2 (3), FASTA
>9S2E_2 Splicing factor ESS-2 homolog (chains 3)
METPGASASSLLLPAASRPPRKREAGEAGAATSKQRVLDEEEYIEGLQTVIQRDFFPDVE
KLQAQKEYLEAEENGDLERMRQIAIKFGSALGKMSREPPPPYVTPATFETPEVHAGTGVV
GNKPRPRGRGLEDGEAGEEEEKEPLPSLDVFLSRYTSEDNASFQEIMEVAKERSRARHAW
LYQAEEEFEKRQKDNLELPSAEHQAIESSQASVETWKYKAKNSLMYYPEGVPDEEQLFKK
PRQVVHKNTRFLRDPFSQALSRCQLQQAAALNAQHKQGKVGPDGKELIPQESPRVGGFGF
VATPSPAPGVNESPMMTWGEVENTPLRVEGSETPYVDRTPGPAFKILEPGRRERLGLKMA
NEAAAKNRAKKQEALRRVTENLASLTPKGLSPAMSPALQRLVSRTASKYTDRALRASYTP
SPARSTHLKTPASGLQTPTSTPAPGSATRTPLTQDPASITDNLLQLPARRKASDFF
Sequence of entity 3 (5), FASTA
>9S2E_3 U5 snRNA (chains 5)
AUACUCUGGUUUCUCUUCAGAUCGCAUAAAUCUUUCGCCUUUUACUAAAGAUUUCCGUGG
AGAGGAACAACUCUGAGUCUUAACCCAAUUUUUUGAGCCUUGCCUUGGCAAGGCUA
Sequence of entity 4 (6), FASTA
>9S2E_4 U6 snRNA (chains 6)
GUGCUCGCUUCGGCAGCACAUAUACUAAAAUUGGAACGAUACAGAGAAGAUUAGCAUGGC
CCCUGCGCAAGGAUGACACGCAAAUUCGUGAAGCGUUCCAUAUUUU
Sequence of entity 5 (A), FASTA
>9S2E_5 Pre-mRNA-processing-splicing factor 8 (chains A)
MAGVFPYRGPGNPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRYAEKRKFGFVDAQKED
MPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLY
HITGAISFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYAD
NILDVEPLEAIQLELDPEEDAPVLDWFYDHQPLRDSRKYVNGSTYQRWQFTLPMMSTLYR
LANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDIN
KIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPIS
HRHSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRR
ALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATK
FFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRF
GNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMR
QIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQF
EGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCW
KANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLT
RLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLI
LALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMD
LYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGIN
NLQDVWETSEGECNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYK
DMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDIATE
AAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARM
RLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKC
RTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIV
NKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPK
ELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFI
DSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRT
DFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEG
LFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGI
FMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSY
KMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYA
RAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPAL
YVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTT
KPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQ
IIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLY
DDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTITEPHHIWPTLTDEEWIKVE
VQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLT
ATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKET
GYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLP
GQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTP
GSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWN
YNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA
Sequence of entity 6 (B), FASTA
>9S2E_6 U5 small nuclear ribonucleoprotein 200 kDa helicase (chains B)
MADVTARSLQYEYKANSNLVLQADRSLIDRTRRDEPTGEVLSLVGKLEGTRMGDKAQRTK
PQMQEERRAKRRKRDEDRHDINKMKGYTLLSEGIDEMVGIIYKPKTKETRETYEVLLSFI
QAALGDQPRDILCGAADEVLAVLKNEKLRDKERRKEIDLLLGQTDDTRYHVLVNLGKKIT
DYGGDKEIQNMDDNIDETYGVNVQFESDEEEGDEDVYGEVREEASDDDMEGDEAVVRCTL
SANLVASGELMSSKKKDLHPRDIDAFWLQRQLSRFYDDAIVSQKKADEVLEILKTASDDR
ECENQLVLLLGFNTFDFIKVLRQHRMMILYCTLLASAQSEAEKERIMGKMEADPELSKFL
YQLHETEKEDLIREERSRRERVRQSRMDTDLETMDLDQGGEALAPRQVLDLEDLVFTQGS
HFMANKRCQLPDGSFRRQRKGYEEVHVPALKPKPFGSEEQLLPVEKLPKYAQAGFEGFKT
LNRIQSKLYRAALETDENLLLCAPTGAGKTNVALMCMLREIGKHINMDGTINVDDFKIIY
IAPMRSLVQEMVGSFGKRLATYGITVAELTGDHQLCKEEISATQIIVCTPEKWDIITRKG
GERTYTQLVRLIILDEIHLLHDDRGPVLEALVARAIRNIEMTQEDVRLIGLSATLPNYED
VATFLRVDPAKGLFYFDNSFRPVPLEQTYVGITEKKAIKRFQIMNEIVYEKIMEHAGKNQ
VLVFVHSRKETGKTARAIRDMCLEKDTLGLFLREGSASTEVLRTEAEQCKNLELKDLLPY
GFAIHHAGMTRVDRTLVEDLFADKHIQVLVSTATLAWGVNLPAHTVIIKGTQVYSPEKGR
WTELGALDILQMLGRAGRPQYDTKGEGILITSHGELQYYLSLLNQQLPIESQMVSKLPDM
LNAEIVLGNVQNAKDAVNWLGYAYLYIRMLRSPTLYGISHDDLKGDPLLDQRRLDLVHTA
ALMLDKNNLVKYDKKTGNFQVTELGRIASHYYITNDTVQTYNQLLKPTLSEIELFRVFSL
SSEFKNITVREEEKLELQKLLERVPIPVKESIEEPSAKINVLLQAFISQLKLEGFALMAD
MVYVTQSAGRLMRAIFEIVLNRGWAQLTDKTLNLCKMIDKRMWQSMCPLRQFRKLPEEVV
KKIEKKNFPFERLYDLNHNEIGELIRMPKMGKTIHKYVHLFPKLELSVHLQPITRSTLKV
ELTITPDFQWDEKVHGSSEAFWILVEDVDSEVILHHEYFLLKAKYAQDEHLITFFVPVFE
PLPPQYFIRVVSDRWLSCETQLPVSFRHLILPEKYPPPTELLDLQPLPVSALRNSAFESL
YQDKFPFFNPIQTQVFNTVYNSDDNVFVGAPTGSGKTICAEFAILRMLLQSSEGRCVYIT
PMEALAEQVYMDWYEKFQDRLNKKVVLLTGETSTDLKLLGKGNIIISTPEKWDILSRRWK
QRKNVQNINLFVVDEVHLIGGENGPVLEVICSRMRYISSQIERPIRIVALSSSLSNAKDV
AHWLGCSATSTFNFHPNVRPVPLELHIQGFNISHTQTRLLSMAKPVYHAITKHSPKKPVI
VFVPSRKQTRLTAIDILTTCAADIQRQRFLHCTEKDLIPYLEKLSDSTLKETLLNGVGYL
HEGLSPMERRLVEQLFSSGAIQVVVASRSLCWGMNVAAHLVIIMDTQYYNGKIHAYVDYP
IYDVLQMVGHANRPLQDDEGRCVIMCQGSKKDFFKKFLYEPLPVESHLDHCMHDHFNAEI
VTKTIENKQDAVDYLTWTFLYRRMTQNPNYYNLQGISHRHLSDHLSELVEQTLSDLEQSK
CISIEDEMDVAPLNLGMIAAYYYINYTTIELFSMSLNAKTKVRGLIEIISNAAEYENIPI
RHHEDNLLRQLAQKVPHKLNNPKFNDPHVKTNLLLQAHLSRMQLSAELQSDTEEILSKAI
RLIQACVDVLSSNGWLSPALAAMELAQMVTQAMWSKDSYLKQLPHFTSEHIKRCTDKGVE
SVFDIMEMEDEERNALLQLTDSQIADVARFCNRYPNIELSYEVVDKDSIRSGGPVVVLVQ
LEREEEVTGPVIAPLFPQKREEGWWVVIGDAKSNSLISIKRLTLQQKAKVKLDFVAPATG
AHNYTLYFMSDAYMGCDQEYKFSVDVKEAETDSDSD
Sequence of entity 7 (C), FASTA
>9S2E_7 116 kDa U5 small nuclear ribonucleoprotein component (chains C)
MDTDLYDEFGNYIGPELDSDEDDDELGRETKDLDEMDDDDDDDDVGDHDDDHPGMEVVLH
EDKKYYPTAEEVYGPEVETIVQEEDTQPLTEPIIKPVKTKKFTLMEQTLPVTVYEMDFLA
DLMDNSELIRNVTLCGHLHHGKTCFVDCLIEQTHPEIRKRYDQDLCYTDILFTEQERGVG
IKSTPVTVVLPDTKGKSYLFNIMDTPGHVNFSDEVTAGLRISDGVVLFIDAAEGVMLNTE
RLIKHAVQERLAVTVCINKIDRLILELKLPPTDAYYKLRHIVDEVNGLISMYSTDENLIL
SPLLGNVCFSSSQYSICFTLGSFAKIYADTFGDINYQEFAKRLWGDIYFNPKTRKFTKKA
PTSSSQRSFVEFILEPLYKILAQVVGDVDTSLPRTLDELGIHLTKEELKLNIRPLLRLVC
KKFFGEFTGFVDMCVQHIPSPKVGAKPKIEHTYTGGVDSDLGEAMSDCDPDGPLMCHTTK
MYSTDDGVQFHAFGRVLSGTIHAGQPVKVLGENYTLEDEEDSQICTVGRLWISVARYHIE
VNRVPAGNWVLIEGVDQPIVKTATITEPRGNEEAQIFRPLKFNTTSVIKIAVEPVNPSEL
PKMLDGLRKVNKSYPSLTTKVEESGEHVILGTGELYLDCVMHDLRKMYSEIDIKVADPVV
TFCETVVETSSLKCFAETPNKKNKITMIAEPLEKGLAEDIENEVVQITWNRKKLGEFFQT
KYDWDLLAARSIWAFGPDATGPNILVDDTLPSEVDKALLGSVKDSIVQGFQWGTREGPLC
DELIRNVKFKILDAVVAQEPLHRGGGQIIPTARRVVYSAFLMATPRLMEPYYFVEVQAPA
DCVSAVYTVLARRRGHVTQDAPIPGSPLYTIKAFIPAIDSFGFETDLRTHTQGQAFSLSV
FHHWQIVPGDPLDKSIVIRPLEPQPAPHLAREFMIKTRRRKGLSEDVSISKFFDDPMLLE
LAKQDVVLNYPM
Sequence of entity 8 (D), FASTA
>9S2E_8 Pre-mRNA-splicing factor ISY1 homolog (chains D)
MARNAEKAMTALARFRQAQLEEGKVKERRPFLASECTELPKAEKWRRQIIGEISKKVAQI
QNAGLGEFRIRDLNDEINKLLREKGHWEVRIKELGGPDYGKVGPKMLDHEGKEVPGNRGY
KYFGAAKDLPGVRELFEKEPLPPPRKTRAELMKAIDFEYYGYLDEDDGVIVPLEQEYEKK
LRAELVEKWKAEREARLARGEKEEEEEEEEEINIYAVTEEESDEEGSQEKGGDDSQQKFI
AHVPVPSQQEIEEALVRRKKMELLQKYASETLQAQSEEARRLLGY
Sequence of entity 9 (DX), FASTA
>9S2E_9 ATP-dependent RNA helicase DHX15 (chains DX)
MSKRHRLDLGEDYPSGKKRAGTDGKDRDRDRDREDRSKDRDRERDRGDREREREKEKEKE
LRASTNAMLISAGLPPLKASHSAHSTHSAHSTHSTHSAHSTHAGHAGHTSLPQCINPFTN
LPHTPRYYDILKKRLQLPVWEYKDRFTDILVRHQSFVLVGETGSGKTTQIPQWCVEYMRS
LPGPKRGVACTQPRRVAAMSVAQRVADEMDVMLGQEVGYSIRFEDCSSAKTILKYMTDGM
LLREAMNDPLLERYGVIILDEAHERTLATDILMGVLKEVVRQRSDLKVIVMSATLDAGKF
QIYFDNCPLLTIPGRTHPVEIFYTPEPERDYLEAAIRTVIQIHMCEEEEGDLLLFLTGQE
EIDEACKRIKREVDDLGPEVGDIKIIPLYSTLPPQQQQRIFEPPPPKKQNGAIGRKVVVS
TNIAETSLTIDGVVFVIDPGFAKQKVYNPRIRVESLLVTAISKASAQQRAGRAGRTRPGK
CFRLYTEKAYKTEMQDNTYPEILRSNLGSVVLQLKKLGIDDLVHFDFMDPPAPETLMRAL
ELLNYLAALNDDGDLTELGSMMAEFPLDPQLAKMVIASCDYNCSNEVLSITAMLSVPQCF
VRPTEAKKAADEAKMRFAHIDGDHLTLLNVYHAFKQNHESVQWCYDNFINYRSLMSADNV
RQQLSRIMDRFNLPRRSTDFTSRDYYINIRKALVTGYFMQVAHLERTGHYLTVKDNQVVQ
LHPSTVLDHKPEWVLYNEFVLTTKNYIRTCTDIKPEWLVKIAPQYYDMSNFPQCEAKRQL
DRIIAKLQSKEYSQY
Sequence of entity 10 (E), FASTA
>9S2E_10 U5 small nuclear ribonucleoprotein 40 kDa protein (chains E)
MIEQQKRKGPELPLVPVKRQRHELLLGAGSGPGAGQQQATPGALLQAGPPRCSSLQAPIM
LLSGHEGEVYCCKFHPNGSTLASAGFDRLILLWNVYGDCDNYATLKGHSGAVMELHYNTD
GSMLFSASTDKTVAVWDSETGERVKRLKGHTSFVNSCYPARRGPQLVCTGSDDGTVKLWD
IRKKAAIQTFQNTYQVLAVTFNDTSDQIISGGIDNDIKVWDLRQNKLTYTMRGHADSVTG
LSLSSEGSYLLSNAMDNTVRVWDVRPFAPKERCVKIFQGNVHNFEKNLLRCSWSPDGSKI
AAGSADRFVYVWDTTSRRILYKLPGHAGSINEVAFHPDEPIIISASSDKRLYMGEIQ
Sequence of entity 11 (GC), FASTA
>9S2E_11 Intron Large complex component GCFC2 (chains GC)
MAHRPKRTFRQRAADSSDSDGAEESPAEPGAPRELPVPGSAEEEPPSGGGRAQVAGLPHR
VRGPRGRGRVWASSRRATKAAPRADEGSESRTLDVSTDEEDKIHHSSESKDDQGLSSDSS
SSLGEKELSSTVKIPDAAFIQAARRKRELARAQDDYISLDVQHTSSISGMKRESEDDPES
EPDDHEKRIPFTLRPQTLRQRMAEESISRNEETSEESQEDEKQDTWEQQQMRKAVKIIEE
RDIDLSCGNGSSKVKKFDTSISFPPVNLEIIKKQLNTRLTLLQETHRSHLREYEKYVQDV
KSSKSTIQNLESSSNQALNCKFYKSMKIYVENLIDCLNEKIINIQEIESSMHALLLKQAM
TFMKRRQDELKHESTYLQQLSRKDETSTSGNFSVDEKTQWILEEIESRRTKRRQARVLSG
NCNHQEGTSSDDELPSAEMIDFQKSQGDILQKQKKVFEEVQDDFCNIQNILLKFQQWREK
FPDSYYEAFISLCIPKLLNPLIRVQLIDWNPLKLESTGLKEMPWFKSVEEFMDSSVEDSK
KESSSDKKVLSAIINKTIIPRLTDFVEFLWDPLSTSQTTSLITHCRVILEEHSTCENEVS
KSRQDLLKSIVSRMKKAVEDDVFIPLYPKSAVENKTSPHSKFQERQFWSGLKLFRNILLW
NGLLTDDTLQELGLGKLLNRYLIIALLNATPGPDVVKKCNQVAACLPEKWFENSAMRTSI
PQLENFIQFLLQSAHKLSRSEFRDEVEEIILILVKIKALNQAESFIGEHHLDHLKSLIKE
D
Sequence of entity 12 (I), FASTA
>9S2E_12 Pre-mRNA-splicing factor SYF1 (chains I)
MVVMARLSRPERPDLVFEEEDLPYEEEIMRNQFSVKCWLRYIEFKQGAPKPRLNQLYERA
LKLLPCSYKLWYRYLKARRAQVKHRCVTDPAYEDVNNCHERAFVFMHKMPRLWLDYCQFL
MDQGRVTHTRRTFDRALRALPITQHSRIWPLYLRFLRSHPLPETAVRGYRRFLKLSPESA
EEYIEYLKSSDRLDEAAQRLATVVNDERFVSKAGKSNYQLWHELCDLISQNPDKVQSLNV
DAIIRGGLTRFTDQLGKLWCSLADYYIRSGHFEKARDVYEEAIRTVMTVRDFTQVFDSYA
QFEESMIAAKMETASELGREEEDDVDLELRLARFEQLISRRPLLLNSVLLRQNPHHVHEW
HKRVALHQGRPREIINTYTEAVQTVDPFKATGKPHTLWVAFAKFYEDNGQLDDARVILEK
ATKVNFKQVDDLASVWCQCGELELRHENYDEALRLLRKATALPARRAEYFDGSEPVQNRV
YKSLKVWSMLADLEESLGTFQSTKAVYDRILDLRIATPQIVINYAMFLEEHKYFEESFKA
YERGISLFKWPNVSDIWSTYLTKFIARYGGRKLERARDLFEQALDGCPPKYAKTLYLLYA
QLEEEWGLARHAMAVYERATRAVEPAQQYDMFNIYIKRAAEIYGVTHTRGIYQKAIEVLS
DEHAREMCLRFADMECKLGEIDRARAIYSFCSQICDPRTTGAFWQTWKDFEVRHGNEDTI
KEMLRIRRSVQATYNTQVNFMASQMLKVSGSATGTVSDLAPGQSGMDDMKLLEQRAEQLA
AEAERDQPLRAQSKILFVRSDASREELAELAQQVNPEEIQLGEDEDEDEMDLEPNEVRLE
QQSVPAAVFGSLKED
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| ZN | Zinc ion | Zn | 5 |
| GTP | Guanosine-5'-triphosphate | C10 H16 N5 O14 P3 | 1 |
| IHP | Inositol hexakisphosphate | C6 H18 O24 P6 | 1 |
| MG | Magnesium ion | Mg | 7 |
Primary citation
Mechanisms of spliceosome termination. Boreikaite, V., Vorlaender, M.K., Phillips, A.W. et al. To be published.
Other PDB entries of the same protein (UniProt Q96DF8 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 8C6J 2.8 Å, Human spliceosomal PM5 C* complex
- 9XU3 3.0 Å, Human minor spliceosome exon-ligation-ready C* complex (prior to step-II)
- 9FMD 3.3 Å, Integrative model of the human post-catalytic spliceosome (P-complex)
- 8RO2 3.5 Å, Integrative Structure of the human intron lariat Spliceosome (ILS'')
Browse structure collections
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