7AS8: Rqc2 homolog RqcH
Bacillus subtilis ribosome quality control complex state B. Ribosomal 50S subunit with P-tRNA, RqcH, and RqcP/YabO. Determined by electron microscopy at 2.9 Å resolution. Released 9 Dec 2020.
- Method
- Electron microscopy
- Resolution
- 2.9 Å
- Organisms
- Bacillus subtilis (strain 168), Bacillus subtilis subsp. subtilis str. 168
- Chains
- 33
- Atoms
- 93,801
- Mol. weight
- 1460.68 kDa
- Released
- 9 Dec 2020
Explore 7AS8 in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
7AS8 contains 128 α-helices and 241 β-strands across 30 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain 0: 18 helices, 27 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 5-16 | 12 | |
| β-strand | 22 | 1 | 1 |
| β-strand | 23-31 | 9 | 2 |
| β-strand | 34-41 | 8 | 2 |
| β-strand | 44-51 | 8 | 2 |
| β-strand | 58-60 | 3 | 2 |
| α-helix | 74-83 | 10 | |
| β-strand | 87-92 | 6 | 1 |
| β-strand | 99-107 | 9 | 1 |
| β-strand | 113-121 | 9 | 1 |
| β-strand | 128-133 | 6 | 1 |
| β-strand | 138 | 1 | 3 |
| β-strand | 139-142 | 4 | 1 |
| α-helix | 148-150 | 3 | |
| β-strand | 161 | 1 | 3 |
| α-helix | 162 | 1 | |
| α-helix | 180-184 | 5 | |
| α-helix | 187-189 | 3 | |
| α-helix | 192-195 | 4 | |
| α-helix | 205-212 | 8 | |
| α-helix | 224-235 | 12 | |
| β-strand | 242-245 | 4 | 4 |
| β-strand | 250-253 | 4 | 4 |
| β-strand | 263-266 | 4 | 4 |
| α-helix | 270-316 | 47 | |
| α-helix | 322-334 | 13 | |
| β-strand | 345-349 | 5 | 5 |
| β-strand | 358-362 | 5 | 5 |
| α-helix | 369-412 | 44 | |
| α-helix | 416-428 | 13 | |
| β-strand | 451-454 | 4 | 6 |
| α-helix | 456-458 | 3 | |
| β-strand | 460-464 | 5 | 6 |
| α-helix | 467-476 | 10 | |
| β-strand | 483-487 | 5 | 6 |
| β-strand | 492 | 1 | 7 |
| β-strand | 494-497 | 4 | 6 |
| α-helix | 504-516 | 13 | |
| β-strand | 518 | 1 | 7 |
| β-strand | 526-532 | 7 | 6 |
| α-helix | 533-535 | 3 | |
| β-strand | 536 | 1 | 8 |
| β-strand | 549 | 1 | 8 |
| β-strand | 552-557 | 6 | 6 |
| α-helix | 562-564 | 3 | |
Chain 1: 4 helices, 7 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 2 | 1 | 9 |
| α-helix | 3-9 | 7 | |
| α-helix | 16-25 | 10 | |
| β-strand | 29-30 | 2 | 10 |
| β-strand | 33-34 | 2 | 10 |
| β-strand | 40 | 1 | 9 |
| β-strand | 46-51 | 6 | 10 |
| β-strand | 54-60 | 7 | 10 |
| α-helix | 69-72 | 4 | |
| α-helix | 73-75 | 3 | |
| β-strand | 76-82 | 7 | 10 |
Chain a: 1 helix, 8 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 24-26 | 3 | |
| β-strand | 30-31 | 2 | 76 |
| β-strand | 38-39 | 2 | 77 |
| β-strand | 44-47 | 4 | 76 |
| β-strand | 53-54 | 2 | 77 |
| β-strand | 59-61 | 3 | 76 |
| β-strand | 67-69 | 3 | 76 |
| β-strand | 73-79 | 7 | 77 |
| β-strand | 85-90 | 6 | 77 |
Chain b: 2 helices, 6 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 13-18 | 6 | 78 |
| α-helix | 20-22 | 3 | |
| β-strand | 24-29 | 6 | 78 |
| β-strand | 33-36 | 4 | 79 |
| β-strand | 39 | 1 | 80 |
| β-strand | 44 | 1 | 80 |
| β-strand | 47-50 | 4 | 79 |
| α-helix | 51-54 | 4 | |
Chain c: 3 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 2-7 | 6 | |
| α-helix | 13-34 | 22 | |
| α-helix | 41-64 | 24 | |
Chain d: 2 helices, 3 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 3-8 | 6 | 11 |
| α-helix | 17-26 | 10 | |
| β-strand | 35-38 | 4 | 11 |
| α-helix | 41-50 | 10 | |
| β-strand | 54-58 | 5 | 11 |
Chain E: 2 helices, 24 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 3-5 | 3 | 16 |
| β-strand | 17-19 | 3 | 16 |
| β-strand | 34-35 | 2 | 17 |
| β-strand | 43 | 1 | 18 |
| β-strand | 49 | 1 | 18 |
| β-strand | 54 | 1 | 19 |
| β-strand | 62-63 | 2 | 17 |
| β-strand | 76-83 | 8 | 20 |
| β-strand | 90-96 | 7 | 20 |
| β-strand | 101-105 | 5 | 20 |
| β-strand | 107 | 1 | 21 |
| β-strand | 115-116 | 2 | 20 |
| β-strand | 129-131 | 3 | 22 |
| β-strand | 140-142 | 3 | 22 |
| β-strand | 162-163 | 2 | 22 |
| β-strand | 164-167 | 4 | 23 |
| β-strand | 171-176 | 6 | 23 |
| β-strand | 180-185 | 6 | 23 |
| β-strand | 189-192 | 4 | 22 |
| β-strand | 195 | 1 | 21 |
| α-helix | 208-213 | 6 | |
| β-strand | 216 | 1 | 19 |
| α-helix | 217-218 | 2 | |
| β-strand | 247 | 1 | 24 |
| β-strand | 253 | 1 | 24 |
| β-strand | 270 | 1 | 23 |
Chain f: 3 helices, 6 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 7-9 | 3 | |
| α-helix | 10-16 | 7 | |
| α-helix | 23-25 | 3 | |
| β-strand | 27-29 | 3 | 12 |
| β-strand | 36-38 | 3 | 12 |
| β-strand | 41 | 1 | 57 |
| β-strand | 43 | 1 | 13 |
| β-strand | 48-49 | 2 | 13 |
| β-strand | 52-53 | 2 | 13 |
22 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Rqc2 homolog RqcH | 0 | protein | 597 | Bacillus subtilis (strain 168) | O34693 (AlphaFold model) |
| Uncharacterized protein YabO | 1 | protein | 86 | Bacillus subtilis (strain 168) | P37557 (AlphaFold model) |
| tRNA-Ala-1-1 | 2 | RNA | 76 | Bacillus subtilis subsp. subtilis str. 168 | |
| 23S rRNA | A | RNA | 2926 | Bacillus subtilis subsp. subtilis str. 168 | |
| 5s rRNA | B | RNA | 119 | Bacillus subtilis subsp. subtilis str. 168 | |
| 50S ribosomal protein L2 | E | protein | 277 | Bacillus subtilis (strain 168) | P42919 (AlphaFold model) |
| 50S ribosomal protein L3 | F | protein | 209 | Bacillus subtilis (strain 168) | P42920 (AlphaFold model) |
| 50S ribosomal protein L4 | G | protein | 207 | Bacillus subtilis (strain 168) | P42921 |
| 50S ribosomal protein L5 | H | protein | 179 | Bacillus subtilis (strain 168) | P12877 |
| 50S ribosomal protein L6 | I | protein | 179 | Bacillus subtilis (strain 168) | P46898 |
| 50S ribosomal protein L11 | K | protein | 141 | Bacillus subtilis (strain 168) | Q06796 |
| 50S ribosomal protein L10 | L | protein | 166 | Bacillus subtilis (strain 168) | P42923 |
21 more molecules are not listed.
Sequence of entity 1 (0), FASTA
>7AS8_1 Rqc2 homolog RqcH (chains 0)
MSFDGMFTYGMTHELNEKIMGGRITKIHQPYKHDVIFHIRAKGKNQKLLLSAHPSYSRVH
ITAQAYENPSEPPMFCMLLRKHIEGGFIEKIEQAGLDRIMIFHIKSRNEIGDETVRKLYV
EIMGRHSNIILTDAAENVIIDGLKHLSPSMNSYRTVLPGQDYKLPPAQDKISPLEASEDD
ILRHLSFQEGRLDKQIVDHFSGVSPLFAKEAVHRAGLANKVTLPKALLALFAEVKEHRFI
PNITTVNGKEYFYLLELTHLKGEARRFDSLSELLDRFYFGKAERDRVKQQAQDLERFVVN
ERKKNANKIKKLEKTLEYSENAKEFQLYGELLTANLYMLKKGDKQAEVINYYDEESPTIT
IPLNPNKTPSENAQAYFTKYQKAKNSVAVVEEQIRLAQEEIEYFDQLIQQLSSASPRDIS
EIREELVEGKYLRPKQQKGQKKQKPHNPVLETYESTSGLTILVGKNNRQNEYLTTRVAAR
DDIWLHTKDIPGSHVVIRSSEPDEQTIMEAATIAAYFSKAKDSSSVPVDYTKIRHVKKPN
GAKPGFVTYDSQHTVFVTPDADTVIKLKKSGSGGDYKDHDGDYKDHDIDYKDDDDKG
Sequence of entity 2 (1), FASTA
>7AS8_2 Uncharacterized protein YabO (chains 1)
MRLDKFLKVSRLIKRRTLAKEVADQGRISINGNQAKASSDVKPGDELTVRFGQKLVTVQV
NELKDTTKKEEAANMYTILKEEKLGE
Sequence of entity 3 (2), FASTA
>7AS8_3 tRNA-Ala-1-1 (chains 2)
GGGGCCUUAGCUCAGCUGGGAGAGCGCCUGCUUUGCACGCAGGAGGUCAGCGGUUCGAUC
CCGCUAGGCUCCACCA
Sequence of entity 4 (A), FASTA
>7AS8_4 23S rRNA (chains A)
GGUUAAGUUAGAAAGGGCGCACGGUGGAUGCCUUGGCACUAGGAGCCGAUGAAGGACGGG
ACGAACACCGAUAUGCUUCGGGGAGCUGUAAGCAAGCUUUGAUCCGGAGAUUUCCGAAUG
GGGAAACCCACCACUCGUAAUGGAGUGGUAUCCAUAUCUGAAUUCAUAGGAUAUGAGAAG
GCAGACCCGGGGAACUGAAACAUCUAAGUACCCGGAGGAAGAGAAAGCAAAUGCGAUUCC
CUGAGUAGCGGCGAGCGAAACGGGAUCAGCCCAAACCAAGAGGCUUGCCUCUUGGGGUUG
UAGGACACUCUGUACGGAGUUACAAAGGAACGAGGUAGAUGAAGAGGUCUGGAAAGGCCC
GCCAUAGGAGGUAACAGCCCUGUAGUCAAAACUUCGUUCUCUCCUGAGUGGAUCCUGAGU
ACGGCGGAACACGUGAAAUUCCGUCGGAAUCCGGGAGGACCAUCUCCCAAGGCUAAAUAC
UCCCUAGUGACCGAUAGUGAACCAGUACCGUGAGGGAAAGGUGAAAAGCACCCCGGAAGG
GGAGUGAAAGAGAUCCUGAAACCGUGUGCCUACAAGUAGUCAGAGCCCGUUAACGGGUGA
UGGCGUGCCUUUUGUAGAAUGAACCGGCGAGUUACGAUCUCGUGCAAGGUUAAGCAGAAG
AUGCGGAGCCGCAGCGAAAGCGAGUCUGAAUAGGGCGCAUGAGUACGUGGUCGUAGACCC
GAAACCAGGUGAUCUACCCAUGUCCAGGGUGAAGUUCAGGUAACACUGAAUGGAGGCCCG
AACCCACGCACGUUGAAAAGUGCGGGGAUGAGGUGUGGGUAGGGGUGAAAUGCCAAUCGA
ACCUGGAGAUAGCUGGUUCUCUCCGAAAUAGCUUUAGGGCUAGCCUCAAGGUAAGAGUCU
UGGAGGUAGAGCACUGAUUGGACUAGGGGCCCCUACCGGGUUACCGAAUUCAGUCAAACU
CCGAAUGCCAAUGACUUAUCCUUGGGAGUCAGACUGCGAGUGAUAAGAUCCGUAGUCGAA
AGGGAAACAGCCCAGACCGCCAGCUAAGGUCCCAAAGUAUACGUUAAGUGGAAAAGGAUG
UGGAGUUGCUUAGACAACCAGGAUGUUGGCUUAGAAGCAGCCACCAUUUAAAGAGUGCGU
AAUAGCUCACUGGUCGAGUGACUCUGCGCCGAAAAUGUACCGGGGCUAAACGUAUCACCG
AAGCUGCGGACUGUUCUUCGAACAGUGGUAGGAGAGCGUUCUAAGGGCUGUGAAGCCAGA
CCGGAAGGACUGGUGGAGCGCUUAGAAGUGAGAAUGCCGGUAUGAGUAGCGAAAGAGGGG
UGAGAAUCCCCUCCACCGAAUGCCUAAGGUUUCCUGAGGAAGGCUCGUCCGCUCAGGGUU
AGUCGGGACCUAAGCCGAGGCCGAAAGGCGUAGGCGAUGGACAACAGGUUGAUAUUCCUG
UACCACCUCCUCACCAUUUGAGCAAUGGGGGGACGCAGGAGGAUAGGGUAAGCGCGGUAU
UGGAUAUCCGCGUCCAAGCAGUUAGGCUGGGAAAUAGGCAAAUCCGUUUCCCAUAAGGCU
GAGCUGUGAUGGCGAGCGAAAUAUAGUAGCGAAGUUCCUGAUUCCACACUGCCAAGAAAA
GCCUCUAGCGAGGUGAGAGGUGCCCGUACCGCAAACCGACACAGGUAGGCGAGGAGAGAA
UCCUAAGGUGAUCGAGAGAACUCUCGUUAAGGAACUCGGCAAAAUGACCCCGUAACUUCG
GGAGAAGGGGUGCUCUGUUAGGGUGCAAGCCCGAGAGAGCCGCAGUGAAUAGGCCCAGGC
GACUGUUUAGCAAAAACACAGGUCUCUGCGAAGCCGUAAGGCGAAGUAUAGGGGCUGACG
CCUGCCCGGUGCUGGAAGGUUAAGAGGAGCGCUUAGCGUAAGCGAAGGUGCGAAUUGAAG
CCCCAGUAAACGGCGGCCGUAACUAUAACGGUCCUAAGGUAGCGAAAUUCCUUGUCGGGU
AAGUUCCGACCCGCACGAAAGGCGCAACGAUCUGGGCACUGUCUCAACGAGAGACUCGGU
GAAAUUAUAGUACCUGUGAAGAUGCAGGUUACCCGCGACAGGACGGAAAGACCCCGUGGA
GCUUUACUGCAGCCUGAUAUUGAAUGUUGGUACAGCUUGUACAGGAUAGGUAGGAGCCUU
GGAAACCGGAGCGCCAGCUUCGGUGGAGGCAUCGGUGGGAUACUACCCUGGCUGUAUUGA
CCUUCUAACCCGCCGCCCUUAUCGGGCGGGGAGACAGUGUCAGGUGGGCAGUUUGACUGG
GGCGGUCGCCUCCUAAAAGGUAACGGAGGCGCCCAAAGGUUCCCUCAGAAUGGUUGGAAA
UCAUUCGCAGAGUGUAAAGGCACAAGGGAGCUUGACUGCGAGACCUACAAGUCGAGCAGG
GACGAAAGUCGGGCUUAGUGAUCCGGUGGUUCCGCAUGGAAGGGCCAUCGCUCAACGGAU
AAAAGCUACCCCGGGGAUAACAGGCUUAUCUCCCCCAAGAGUCCACAUCGACGGGGAGGU
UUGGCACCUCGAUGUCGGCUCAUCGCAUCCUGGGGCUGUAGUCGGUCCCAAGGGUUGGGC
UGUUCGCCCAUUAAAGCGGUACGCGAGCUGGGUUCAGAACGUCGUGAGACAGUUCGGUCC
CUAUCCGUCGCGGGCGCAGGAAAUUUGAGAGGAGCUGUCCUUAGUACGAGAGGACCGGGA
UGGACGCACCGCUGGUGUACCAGUUGUUCUGCCAAGGGCAUCGCUGGGUAGCUAUGUGCG
GACGGGAUAAGUGCUGAAAGCAUCUAAGCAUGAAGCCCCCCUCAAGAUGAGAUUUCCCAU
UCCGCAAGGAAGUAAGAUCCCUGAAAGAUGAUCAGGUUGAUAGGUCUGAGGUGGAAGUGU
GGCGACACAUGGAGCUGACAGAUACUAAUCGAUCGAGGACUUAACC
Sequence of entity 5 (B), FASTA
>7AS8_5 5s rRNA (chains B)
UUUGGUGGCGAUAGCGAAGAGGUCACACCCGUUCCCAUACCGAACACGGAAGUUAAGCUC
UUCAGCGCCGAUGGUAGUCGGGGGUUUCCCCCUGUGAGAGUAGGACGCCGCCAAGCAAG
Sequence of entity 6 (E), FASTA
>7AS8_6 50S ribosomal protein L2 (chains E)
MAIKKYKPTSNGRRGMTTSDFAEITTDKPEKSLLAPLHKKGGRNNQGKLTVRHQGGGHKR
QYRVIDFKRDKDGIPGRVATVEYDPNRSANIALINYADGEKRYILAPKGIQVGTEIMSGP
EADIKVGNALPLINIPVGTVVHNIELKPGKGGQLVRSAGTSAQVLGKEGKYVLVRLNSGE
VRMILSACRASIGQVGNEQHELINIGKAGRSRWKGIRPTVRGSVMNPNDHPHGGGEGRAP
IGRKSPMSPWGKPTLGFKTRKKKNKSDKFIVRRRKNK
Sequence of entity 7 (F), FASTA
>7AS8_7 50S ribosomal protein L3 (chains F)
MTKGILGRKIGMTQVFAENGDLIPVTVIEAAPNVVLQKKTAENDGYEAIQLGFDDKREKL
SNKPEKGHVAKAETAPKRFVKELRGVEMDAYEVGQEVKVEIFSAGEIVDVTGVSKGKGFQ
GAIKRHGQSRGPMSHGSRYHRRPGSMGPVDPNRVFKGKLLPGRMGGEQITVQNLEIVKVD
AERNLLLIKGNVPGAKKSLITVKSAVKSK
Sequence of entity 8 (G), FASTA
>7AS8_8 50S ribosomal protein L4 (chains G)
MPKVALYNQNGSTAGDIELNASVFGIEPNESVVFDAILMQRASLRQGTHKVKNRSEVRGG
GRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLPKKVRRLAIKSVLSSKVID
NNIIVLEDLTLDTAKTKEMAAILKGLSVEKKALIVTADANEAVALSARNIPGVTVVEANG
INVLDVVNHEKLLITKAAVEKVEEVLA
Sequence of entity 9 (H), FASTA
>7AS8_9 50S ribosomal protein L5 (chains H)
MNRLKEKYNKEIAPALMTKFNYDSVMQVPKIEKIVINMGVGDAVQNAKAIDSAVEELTFI
AGQKPVVTRAKKSIAGFRLREGMPIGAKVTLRGERMYDFLDKLISVSLPRVRDFRGVSKK
SFDGRGNYTLGIKEQLIFPEIDYDKVTKVRGMDIVIVTTANTDEEARELLTQVGMPFQK
Sequence of entity 10 (I), FASTA
>7AS8_10 50S ribosomal protein L6 (chains I)
MSRVGKKLLEIPSDVTVTLNDNNTVAVKGPKGELTRTFHPDMEIKVEDNVLTVARPSDQK
EHRALHGTTRSLLGNMVEGVSKGFERGLELVGVGYRASKSGNKLVLNVGYSHPVEIVPEE
GIEIEVPSQTKVVVKGTDKERVGAIAANIRAVRSPEPYKGKGIRYEGEVVRRKEGKSAK
Sequence of entity 11 (K), FASTA
>7AS8_11 50S ribosomal protein L11 (chains K)
MAKKVVKVVKLQIPAGKANPAPPVGPALGQAGVNIMGFCKEFNARTADQAGLIIPVEISV
YEDRSFTFITKTPPAAVLLKKAAGIESGSGEPNRNKVATVKRDKVREIAETKMPDLNAAD
VEAAMRMVEGTARSMGIVIED
Sequence of entity 12 (L), FASTA
>7AS8_12 50S ribosomal protein L10 (chains L)
MSSAIETKKVVVEEIASKLKESKSTIIVDYRGLNVSEVTELRKQLREANVEFKVYKNTMT
RRAVEQAELNGLNDFLTGPNAIAFSTEDVVAPAKVLNDFAKNHEALEIKAGVIEGKVSTV
EEVKALAELPSREGLLSMLLSVLQAPVRNLALAAKAVAEQKEEQGA
Primary citation
Structural Basis for Bacterial Ribosome-Associated Quality Control by RqcH and RqcP. Crowe-McAuliffe, C., Takada, H., Murina, V. et al. Mol Cell (2021) 81:115. DOI 10.1016/j.molcel.2020.11.002 · PubMed
Other PDB entries of the same protein (UniProt O34693 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 7AQC 2.99 Å, Structure of the bacterial RQC complex (Decoding State)
- 7AQD 3.1 Å, Structure of the bacterial RQC complex (Translocating State)
- 7OPE 3.2 Å, RqcH DR variant bound to 50S-peptidyl-tRNA-RqcP RQC complex (rigid body refinement)
- 8S1U 3.4 Å, YlmH bound to stalled 50S subunits with RqcH and PtRNA
- 7AS9 3.5 Å, Bacillus subtilis ribosome-associated quality control complex state A. Ribosomal 50S…
- 7ASA 3.5 Å, Bacillus subtilis ribosome-associated quality control complex state B, multibody…
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