7AS9: Rqc2 homolog RqcH

Bacillus subtilis ribosome-associated quality control complex state A. Ribosomal 50S subunit with peptidyl tRNA in the A/P position and RqcH. Determined by electron microscopy at 3.5 Å resolution. Released 23 Dec 2020.

Method: Electron microscopy
Resolution: 3.5 Å
Organisms: Bacillus subtilis (strain 168), Bacillus subtilis subsp. subtilis str. 168
Chains: 32
Atoms: 93,137
Mol. weight: 1450.95 kDa
Released: 23 Dec 2020

Explore 7AS9 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7AS9 contains 114 α-helices and 228 β-strands across 29 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain 0: 19 helices, 29 β-strands

Element	Residues	Length	Sheet
α-helix	6-19	14
β-strand	23	1	1
β-strand	24-28	5	2
β-strand	35-41	7	2
β-strand	44-50	7	2
β-strand	60	1	2
α-helix	74-78	5
α-helix	79-83	5
β-strand	86-93	8	1
β-strand	99	1	3
β-strand	100-106	7	1
β-strand	107	1	4
β-strand	113	1	4
β-strand	116	1	1
β-strand	119-120	2	5
β-strand	121	1	3
β-strand	129-130	2	5
β-strand	132-133	2	6
β-strand	138-139	2	6
β-strand	161	1	6
α-helix	162	1
α-helix	180-184	5
α-helix	192-199	8
α-helix	205-213	9
α-helix	223-236	14
β-strand	242-244	3	7
β-strand	251-253	3	7
β-strand	265	1	7
α-helix	270-273	4
α-helix	275-316	42
α-helix	323-334	12
α-helix	369-413	45
α-helix	416-428	13
β-strand	451-454	4	8
β-strand	460-464	5	8
α-helix	467-470	4
α-helix	471-477	7
α-helix	479	1
β-strand	483-485	3	8
β-strand	494-497	4	8
α-helix	504-517	14
β-strand	526-530	5	9
β-strand	532	1	8
α-helix	533-535	3
β-strand	536	1	10
β-strand	549	1	10
β-strand	553-557	5	9
α-helix	562-564	3

Chain a: 0 helices, 8 β-strands

Element	Residues	Length	Sheet
β-strand	30-31	2	78
β-strand	38-39	2	79
β-strand	44-47	4	78
β-strand	53-55	3	79
β-strand	59-61	3	78
β-strand	67-69	3	78
β-strand	73-79	7	79
β-strand	85-90	6	79

Chain b: 2 helices, 4 β-strands

Element	Residues	Length	Sheet
β-strand	14	1	80
α-helix	20-22	3
β-strand	28	1	80
β-strand	34-36	3	81
β-strand	47-49	3	81
α-helix	51-54	4

Chain c: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	2-6	5
α-helix	13-34	22
α-helix	41-64	24

Chain d: 2 helices, 3 β-strands

Element	Residues	Length	Sheet
β-strand	3-8	6	11
α-helix	17-26	10
β-strand	35-38	4	11
α-helix	41-50	10
β-strand	54-58	5	11

Chain E: 4 helices, 24 β-strands

Element	Residues	Length	Sheet
β-strand	3-5	3	16
α-helix	6-7	2
β-strand	17-19	3	16
β-strand	34-35	2	17
β-strand	43	1	18
β-strand	49	1	18
β-strand	62-63	2	17
β-strand	76-83	8	19
β-strand	90-95	6	19
β-strand	101-105	5	19
β-strand	107	1	20
β-strand	115-116	2	19
β-strand	130-131	2	21
β-strand	141-144	4	21
β-strand	145	1	22
β-strand	152	1	22
β-strand	162	1	21
β-strand	164-167	4	23
β-strand	171-176	6	23
β-strand	180-185	6	23
β-strand	189-192	4	21
β-strand	195	1	20
α-helix	208-214	7
α-helix	222-224	3
β-strand	247	1	24
β-strand	253	1	24
α-helix	266-268	3
β-strand	270	1	23

Chain f: 1 helix, 5 β-strands

Element	Residues	Length	Sheet
α-helix	10-17	8
β-strand	27-29	3	12
β-strand	36-38	3	12
β-strand	43	1	13
β-strand	48-49	2	13
β-strand	52-53	2	13

Chain F: 5 helices, 14 β-strands

Element	Residues	Length	Sheet
β-strand	4-16	13	26
α-helix	21	1
β-strand	22-29	8	26
α-helix	31-32	2
β-strand	33-38	6	27
β-strand	49-52	4	27
β-strand	53	1	28
α-helix	63-71	9
β-strand	79	1	28
β-strand	82-83	2	27
β-strand	96-97	2	27
β-strand	107-113	7	26
β-strand	118-120	3	29
α-helix	122-126	5
β-strand	162-164	3	29
β-strand	168-180	13	26
β-strand	185-189	5	26
β-strand	199-204	6	26
α-helix	205-206	2

21 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Rqc2 homolog RqcH	0	protein	597	Bacillus subtilis (strain 168)	O34693 (AlphaFold model)
tRNA-Ala-1-1	2	RNA	76	Bacillus subtilis subsp. subtilis str. 168
23S rRNA	A	RNA	2926	Bacillus subtilis subsp. subtilis str. 168
5S rRNA	B	RNA	119	Bacillus subtilis subsp. subtilis str. 168
50S ribosomal protein L2	E	protein	277	Bacillus subtilis (strain 168)	P42919 (AlphaFold model)
50S ribosomal protein L3	F	protein	209	Bacillus subtilis (strain 168)	P42920 (AlphaFold model)
50S ribosomal protein L4	G	protein	207	Bacillus subtilis (strain 168)	P42921 (AlphaFold model)
50S ribosomal protein L5	H	protein	179	Bacillus subtilis (strain 168)	P12877
50S ribosomal protein L6	I	protein	179	Bacillus subtilis (strain 168)	P46898
50S ribosomal protein L11	K	protein	141	Bacillus subtilis (strain 168)	Q06796
50S ribosomal protein L10	L	protein	166	Bacillus subtilis (strain 168)	P42923
50S ribosomal protein L13	N	protein	145	Bacillus subtilis (strain 168)	P70974

20 more molecules are not listed.

Sequence of entity 1 (0), FASTA

>7AS9_1 Rqc2 homolog RqcH (chains 0)
MSFDGMFTYGMTHELNEKIMGGRITKIHQPYKHDVIFHIRAKGKNQKLLLSAHPSYSRVH
ITAQAYENPSEPPMFCMLLRKHIEGGFIEKIEQAGLDRIMIFHIKSRNEIGDETVRKLYV
EIMGRHSNIILTDAAENVIIDGLKHLSPSMNSYRTVLPGQDYKLPPAQDKISPLEASEDD
ILRHLSFQEGRLDKQIVDHFSGVSPLFAKEAVHRAGLANKVTLPKALLALFAEVKEHRFI
PNITTVNGKEYFYLLELTHLKGEARRFDSLSELLDRFYFGKAERDRVKQQAQDLERFVVN
ERKKNANKIKKLEKTLEYSENAKEFQLYGELLTANLYMLKKGDKQAEVINYYDEESPTIT
IPLNPNKTPSENAQAYFTKYQKAKNSVAVVEEQIRLAQEEIEYFDQLIQQLSSASPRDIS
EIREELVEGKYLRPKQQKGQKKQKPHNPVLETYESTSGLTILVGKNNRQNEYLTTRVAAR
DDIWLHTKDIPGSHVVIRSSEPDEQTIMEAATIAAYFSKAKDSSSVPVDYTKIRHVKKPN
GAKPGFVTYDSQHTVFVTPDADTVIKLKKSGSGGDYKDHDGDYKDHDIDYKDDDDKG

Sequence of entity 2 (2), FASTA

>7AS9_2 tRNA-Ala-1-1 (chains 2)
GGGGCCUUAGCUCAGCUGGGAGAGCGCCUGCUUUGCACGCAGGAGGUCAGCGGUUCGAUC
CCGCUAGGCUCCACCA

Sequence of entity 3 (A), FASTA

>7AS9_3 23S rRNA (chains A)
GGUUAAGUUAGAAAGGGCGCACGGUGGAUGCCUUGGCACUAGGAGCCGAUGAAGGACGGG
ACGAACACCGAUAUGCUUCGGGGAGCUGUAAGCAAGCUUUGAUCCGGAGAUUUCCGAAUG
GGGAAACCCACCACUCGUAAUGGAGUGGUAUCCAUAUCUGAAUUCAUAGGAUAUGAGAAG
GCAGACCCGGGGAACUGAAACAUCUAAGUACCCGGAGGAAGAGAAAGCAAAUGCGAUUCC
CUGAGUAGCGGCGAGCGAAACGGGAUCAGCCCAAACCAAGAGGCUUGCCUCUUGGGGUUG
UAGGACACUCUGUACGGAGUUACAAAGGAACGAGGUAGAUGAAGAGGUCUGGAAAGGCCC
GCCAUAGGAGGUAACAGCCCUGUAGUCAAAACUUCGUUCUCUCCUGAGUGGAUCCUGAGU
ACGGCGGAACACGUGAAAUUCCGUCGGAAUCCGGGAGGACCAUCUCCCAAGGCUAAAUAC
UCCCUAGUGACCGAUAGUGAACCAGUACCGUGAGGGAAAGGUGAAAAGCACCCCGGAAGG
GGAGUGAAAGAGAUCCUGAAACCGUGUGCCUACAAGUAGUCAGAGCCCGUUAACGGGUGA
UGGCGUGCCUUUUGUAGAAUGAACCGGCGAGUUACGAUCUCGUGCAAGGUUAAGCAGAAG
AUGCGGAGCCGCAGCGAAAGCGAGUCUGAAUAGGGCGCAUGAGUACGUGGUCGUAGACCC
GAAACCAGGUGAUCUACCCAUGUCCAGGGUGAAGUUCAGGUAACACUGAAUGGAGGCCCG
AACCCACGCACGUUGAAAAGUGCGGGGAUGAGGUGUGGGUAGGGGUGAAAUGCCAAUCGA
ACCUGGAGAUAGCUGGUUCUCUCCGAAAUAGCUUUAGGGCUAGCCUCAAGGUAAGAGUCU
UGGAGGUAGAGCACUGAUUGGACUAGGGGCCCCUACCGGGUUACCGAAUUCAGUCAAACU
CCGAAUGCCAAUGACUUAUCCUUGGGAGUCAGACUGCGAGUGAUAAGAUCCGUAGUCGAA
AGGGAAACAGCCCAGACCGCCAGCUAAGGUCCCAAAGUAUACGUUAAGUGGAAAAGGAUG
UGGAGUUGCUUAGACAACCAGGAUGUUGGCUUAGAAGCAGCCACCAUUUAAAGAGUGCGU
AAUAGCUCACUGGUCGAGUGACUCUGCGCCGAAAAUGUACCGGGGCUAAACGUAUCACCG
AAGCUGCGGACUGUUCUUCGAACAGUGGUAGGAGAGCGUUCUAAGGGCUGUGAAGCCAGA
CCGGAAGGACUGGUGGAGCGCUUAGAAGUGAGAAUGCCGGUAUGAGUAGCGAAAGAGGGG
UGAGAAUCCCCUCCACCGAAUGCCUAAGGUUUCCUGAGGAAGGCUCGUCCGCUCAGGGUU
AGUCGGGACCUAAGCCGAGGCCGAAAGGCGUAGGCGAUGGACAACAGGUUGAUAUUCCUG
UACCACCUCCUCACCAUUUGAGCAAUGGGGGGACGCAGGAGGAUAGGGUAAGCGCGGUAU
UGGAUAUCCGCGUCCAAGCAGUUAGGCUGGGAAAUAGGCAAAUCCGUUUCCCAUAAGGCU
GAGCUGUGAUGGCGAGCGAAAUAUAGUAGCGAAGUUCCUGAUUCCACACUGCCAAGAAAA
GCCUCUAGCGAGGUGAGAGGUGCCCGUACCGCAAACCGACACAGGUAGGCGAGGAGAGAA
UCCUAAGGUGAUCGAGAGAACUCUCGUUAAGGAACUCGGCAAAAUGACCCCGUAACUUCG
GGAGAAGGGGUGCUCUGUUAGGGUGCAAGCCCGAGAGAGCCGCAGUGAAUAGGCCCAGGC
GACUGUUUAGCAAAAACACAGGUCUCUGCGAAGCCGUAAGGCGAAGUAUAGGGGCUGACG
CCUGCCCGGUGCUGGAAGGUUAAGAGGAGCGCUUAGCGUAAGCGAAGGUGCGAAUUGAAG
CCCCAGUAAACGGCGGCCGUAACUAUAACGGUCCUAAGGUAGCGAAAUUCCUUGUCGGGU
AAGUUCCGACCCGCACGAAAGGCGCAACGAUCUGGGCACUGUCUCAACGAGAGACUCGGU
GAAAUUAUAGUACCUGUGAAGAUGCAGGUUACCCGCGACAGGACGGAAAGACCCCGUGGA
GCUUUACUGCAGCCUGAUAUUGAAUGUUGGUACAGCUUGUACAGGAUAGGUAGGAGCCUU
GGAAACCGGAGCGCCAGCUUCGGUGGAGGCAUCGGUGGGAUACUACCCUGGCUGUAUUGA
CCUUCUAACCCGCCGCCCUUAUCGGGCGGGGAGACAGUGUCAGGUGGGCAGUUUGACUGG
GGCGGUCGCCUCCUAAAAGGUAACGGAGGCGCCCAAAGGUUCCCUCAGAAUGGUUGGAAA
UCAUUCGCAGAGUGUAAAGGCACAAGGGAGCUUGACUGCGAGACCUACAAGUCGAGCAGG
GACGAAAGUCGGGCUUAGUGAUCCGGUGGUUCCGCAUGGAAGGGCCAUCGCUCAACGGAU
AAAAGCUACCCCGGGGAUAACAGGCUUAUCUCCCCCAAGAGUCCACAUCGACGGGGAGGU
UUGGCACCUCGAUGUCGGCUCAUCGCAUCCUGGGGCUGUAGUCGGUCCCAAGGGUUGGGC
UGUUCGCCCAUUAAAGCGGUACGCGAGCUGGGUUCAGAACGUCGUGAGACAGUUCGGUCC
CUAUCCGUCGCGGGCGCAGGAAAUUUGAGAGGAGCUGUCCUUAGUACGAGAGGACCGGGA
UGGACGCACCGCUGGUGUACCAGUUGUUCUGCCAAGGGCAUCGCUGGGUAGCUAUGUGCG
GACGGGAUAAGUGCUGAAAGCAUCUAAGCAUGAAGCCCCCCUCAAGAUGAGAUUUCCCAU
UCCGCAAGGAAGUAAGAUCCCUGAAAGAUGAUCAGGUUGAUAGGUCUGAGGUGGAAGUGU
GGCGACACAUGGAGCUGACAGAUACUAAUCGAUCGAGGACUUAACC

Sequence of entity 4 (B), FASTA

>7AS9_4 5S rRNA (chains B)
UUUGGUGGCGAUAGCGAAGAGGUCACACCCGUUCCCAUACCGAACACGGAAGUUAAGCUC
UUCAGCGCCGAUGGUAGUCGGGGGUUUCCCCCUGUGAGAGUAGGACGCCGCCAAGCAAG

Sequence of entity 5 (E), FASTA

>7AS9_5 50S ribosomal protein L2 (chains E)
MAIKKYKPTSNGRRGMTTSDFAEITTDKPEKSLLAPLHKKGGRNNQGKLTVRHQGGGHKR
QYRVIDFKRDKDGIPGRVATVEYDPNRSANIALINYADGEKRYILAPKGIQVGTEIMSGP
EADIKVGNALPLINIPVGTVVHNIELKPGKGGQLVRSAGTSAQVLGKEGKYVLVRLNSGE
VRMILSACRASIGQVGNEQHELINIGKAGRSRWKGIRPTVRGSVMNPNDHPHGGGEGRAP
IGRKSPMSPWGKPTLGFKTRKKKNKSDKFIVRRRKNK

Sequence of entity 6 (F), FASTA

>7AS9_6 50S ribosomal protein L3 (chains F)
MTKGILGRKIGMTQVFAENGDLIPVTVIEAAPNVVLQKKTAENDGYEAIQLGFDDKREKL
SNKPEKGHVAKAETAPKRFVKELRGVEMDAYEVGQEVKVEIFSAGEIVDVTGVSKGKGFQ
GAIKRHGQSRGPMSHGSRYHRRPGSMGPVDPNRVFKGKLLPGRMGGEQITVQNLEIVKVD
AERNLLLIKGNVPGAKKSLITVKSAVKSK

Sequence of entity 7 (G), FASTA

>7AS9_7 50S ribosomal protein L4 (chains G)
MPKVALYNQNGSTAGDIELNASVFGIEPNESVVFDAILMQRASLRQGTHKVKNRSEVRGG
GRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLPKKVRRLAIKSVLSSKVID
NNIIVLEDLTLDTAKTKEMAAILKGLSVEKKALIVTADANEAVALSARNIPGVTVVEANG
INVLDVVNHEKLLITKAAVEKVEEVLA

Sequence of entity 8 (H), FASTA

>7AS9_8 50S ribosomal protein L5 (chains H)
MNRLKEKYNKEIAPALMTKFNYDSVMQVPKIEKIVINMGVGDAVQNAKAIDSAVEELTFI
AGQKPVVTRAKKSIAGFRLREGMPIGAKVTLRGERMYDFLDKLISVSLPRVRDFRGVSKK
SFDGRGNYTLGIKEQLIFPEIDYDKVTKVRGMDIVIVTTANTDEEARELLTQVGMPFQK

Sequence of entity 9 (I), FASTA

>7AS9_9 50S ribosomal protein L6 (chains I)
MSRVGKKLLEIPSDVTVTLNDNNTVAVKGPKGELTRTFHPDMEIKVEDNVLTVARPSDQK
EHRALHGTTRSLLGNMVEGVSKGFERGLELVGVGYRASKSGNKLVLNVGYSHPVEIVPEE
GIEIEVPSQTKVVVKGTDKERVGAIAANIRAVRSPEPYKGKGIRYEGEVVRRKEGKSAK

Sequence of entity 10 (K), FASTA

>7AS9_10 50S ribosomal protein L11 (chains K)
MAKKVVKVVKLQIPAGKANPAPPVGPALGQAGVNIMGFCKEFNARTADQAGLIIPVEISV
YEDRSFTFITKTPPAAVLLKKAAGIESGSGEPNRNKVATVKRDKVREIAETKMPDLNAAD
VEAAMRMVEGTARSMGIVIED

Sequence of entity 11 (L), FASTA

>7AS9_11 50S ribosomal protein L10 (chains L)
MSSAIETKKVVVEEIASKLKESKSTIIVDYRGLNVSEVTELRKQLREANVEFKVYKNTMT
RRAVEQAELNGLNDFLTGPNAIAFSTEDVVAPAKVLNDFAKNHEALEIKAGVIEGKVSTV
EEVKALAELPSREGLLSMLLSVLQAPVRNLALAAKAVAEQKEEQGA

Sequence of entity 12 (N), FASTA

>7AS9_12 50S ribosomal protein L13 (chains N)
MRTTPMANASTIERKWLVVDAAGKTLGRLSSEVAAILRGKHKPTYTPHVDTGDHVIIINA
EKIELTGKKLTDKIYYRHTQHPGGLKSRTALEMRTNYPEKMLELAIKGMLPKGSLGRQMF
KKLNVYRGSEHPHEAQKPEVYELRG

Primary citation

Structural Basis for Bacterial Ribosome-Associated Quality Control by RqcH and RqcP. Crowe-McAuliffe, C., Takada, H., Murina, V. et al. Mol Cell (2021) 81:115. DOI 10.1016/j.molcel.2020.11.002 · PubMed

Other PDB entries of the same protein (UniProt O34693 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7AS8 2.9 Å, Bacillus subtilis ribosome quality control complex state B. Ribosomal 50S subunit with…
7AQC 2.99 Å, Structure of the bacterial RQC complex (Decoding State)
7AQD 3.1 Å, Structure of the bacterial RQC complex (Translocating State)
7OPE 3.2 Å, RqcH DR variant bound to 50S-peptidyl-tRNA-RqcP RQC complex (rigid body refinement)
8S1U 3.4 Å, YlmH bound to stalled 50S subunits with RqcH and PtRNA
7ASA 3.5 Å, Bacillus subtilis ribosome-associated quality control complex state B, multibody…

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