Crystal Structure of human Prolyl-tRNA synthetase in complex with NCP26 and L-Proline. Determined by X-ray diffraction at 2.19 Å resolution. Released 12 Jan 2022.
Explore 7BBU in 3D Show helices and sheets RCSB PDB PDBe
7BBU contains 21 α-helices and 32 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 1024-1034 | 11 | |
| β-strand | 1038-1040 | 3 | 1 |
| β-strand | 1047-1049 | 3 | 1 |
| α-helix | 1051-1069 | 19 | |
| β-strand | 1074-1075 | 2 | 2 |
| β-strand | 1081-1082 | 2 | 3 |
| α-helix | 1084-1088 | 5 | |
| β-strand | 1103-1107 | 5 | 3 |
| β-strand | 1110-1118 | 9 | 3 |
| α-helix | 1119 | 1 | |
| α-helix | 1123-1133 | 11 | |
| β-strand | 1136 | 1 | 4 |
| α-helix | 1137-1139 | 3 | |
| β-strand | 1142-1151 | 10 | 2 |
| α-helix | 1157-1158 | 2 | |
| β-strand | 1159 | 1 | 5 |
| β-strand | 1163 | 1 | 5 |
| β-strand | 1166-1176 | 11 | 2 |
| α-helix | 1179-1195 | 17 | |
| α-helix | 1196-1200 | 5 | |
| β-strand | 1206-1209 | 4 | 2 |
| α-helix | 1216 | 1 | |
| β-strand | 1221-1229 | 9 | 2 |
| β-strand | 1234-1245 | 12 | 2 |
| α-helix | 1247-1252 | 6 | |
| β-strand | 1255-1257 | 3 | 4 |
| β-strand | 1265-1267 | 3 | 4 |
| α-helix | 1268 | 1 | |
| β-strand | 1269-1276 | 8 | 2 |
| α-helix | 1278-1287 | 10 | |
| β-strand | 1289 | 1 | 6 |
| β-strand | 1292 | 1 | 6 |
| β-strand | 1304-1308 | 5 | 7 |
| α-helix | 1318-1336 | 19 | |
| β-strand | 1341-1343 | 3 | 7 |
| α-helix | 1351-1361 | 11 | |
| β-strand | 1365-1369 | 5 | 7 |
| α-helix | 1371-1375 | 5 | |
| β-strand | 1378-1383 | 6 | 7 |
| β-strand | 1389-1393 | 5 | 7 |
| α-helix | 1397-1422 | 26 | |
| β-strand | 1424-1426 | 3 | 8 |
| α-helix | 1430-1438 | 9 | |
| β-strand | 1442-1447 | 6 | 8 |
| α-helix | 1451-1462 | 12 | |
| β-strand | 1477-1480 | 4 | 8 |
| β-strand | 1481-1482 | 2 | 2 |
| α-helix | 1493 | 1 | |
| β-strand | 1494 | 1 | 9 |
| α-helix | 1495 | 1 | |
| β-strand | 1501 | 1 | 9 |
| β-strand | 1504-1509 | 6 | 8 |
| β-strand | 1511 | 1 | 2 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| Bifunctional glutamate/proline--tRNA ligase | A | protein | 515 | Homo sapiens | P07814 (AlphaFold model) |
>7BBU_1 Bifunctional glutamate/proline--tRNA ligase (chains A) SMSGAGEGQGPKKQTRLGLEAKKEENLADWYSQVITKSEMIEYHDISGCYILRPWAYAIW EAIKDFFDAEIKKLGVENCYFPMFVSQSALEKEKTHVADFAPEVAWVTRSGKTELAEPIA IRPTSETVMYPAYAKWVQSHRDLPIKLNQWCNVVRWEFKHPQPFLRTREFLWQEGHSAFA TMEEAAEEVLQILDLYAQVYEELLAIPVVKGRKTEKEKFAGGDYTTTIEAFISASGRAIQ GGTSHHLGQNFSKMFEIVFEDPKIPGEKQFAYQNSWGLTTRTIGVMTMVHGDNMGLVLPP RVACVQVVIIPCGITNALSEEDKEALIAKCNDYRRRLLSVNIRVRADLRDNYSPGWKFNH WELKGVPIRLEVGPRDMKSCQFVAVRRDTGEKLTVAENEAETKLQAILEDIQVTLFTRAS EDLKTHMVVANTMEDFQKILDSGKIVQIPFCGEIDCEDWIKKTTARDQDLEPGAPSMGAK SLCIPFKPLCELQPGAKCVCGKNPAKYYTLFGRSY
| ID | Name | Formula | Copies |
|---|---|---|---|
| PRO | Proline | C5 H9 N O2 | 1 |
| MU5 | ~{N}-(2,3-dihydro-1~{H}-inden-2-yl)-3-(piperidin-1-ylcarbonylamino)pyrazine-2-c… | C20 H23 N5 O2 | 1 |
| ZN | Zinc ion | Zn | 1 |
Water and common crystallization additives (EDO, NO3, CL) are not listed.
Crystal Structure of human Prolyl-tRNA synthetase in complex with NCP26 and L-Proline. Johansson, C., Tye, M., Payne, N.C. et al. To be published.
Other PDB entries of the same protein (UniProt P07814 (AlphaFold model), which also has an AlphaFold model), best resolution first:
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