7BG9: The catalytic core lobe of human telomerase

The catalytic core lobe of human telomerase in complex with a telomeric DNA substrate. Determined by electron microscopy at 3.8 Å resolution. Released 28 Apr 2021.

Method: Electron microscopy
Resolution: 3.8 Å
Organisms: Homo sapiens, synthetic construct
Chains: 5
Atoms: 14,233
Mol. weight: 332.37 kDa
Released: 28 Apr 2021

Explore 7BG9 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7BG9 contains 53 α-helices and 33 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 44 helices, 29 β-strands

Element	Residues	Length	Sheet
α-helix	12-21	10
β-strand	24-27	4	1
α-helix	32-35	4
α-helix	48-51	4
β-strand	55-58	4	1
α-helix	79-87	9
α-helix	96-99	4
α-helix	134-144	11
α-helix	151-154	4
β-strand	157-160	4	1
β-strand	168-169	2	1
α-helix	175-178	4
α-helix	326-329	4
α-helix	344-348	5
α-helix	355-365	11
α-helix	384-388	5
α-helix	390-401	12
α-helix	445-452	8
α-helix	455	1
β-strand	456	1	2
α-helix	457	1
α-helix	458-469	12
α-helix	475-478	4
α-helix	481-495	15
α-helix	531-557	27
β-strand	561-564	4	2
β-strand	574-577	4	2
α-helix	578-594	17
α-helix	598	1
β-strand	599-600	2	3
α-helix	605-609	5
β-strand	617	1	4
β-strand	619-625	7	5
β-strand	630-636	7	5
α-helix	654-671	18
α-helix	673-675	3
β-strand	680	1	6
α-helix	684-696	13
β-strand	707-711	5	6
α-helix	722-732	11
β-strand	739	1	7
β-strand	740-746	7	8
β-strand	755-761	7	8
α-helix	773-780	8
α-helix	781-783	3
β-strand	790-792	3	8
α-helix	799	1
β-strand	800	1	7
α-helix	801	1
α-helix	802-814	13
β-strand	816	1	4
β-strand	818-820	3	3
β-strand	823-826	4	3
α-helix	838-853	16
β-strand	862-866	5	6
β-strand	869-874	6	6
α-helix	877-888	12
β-strand	904-906	3	6
β-strand	928-930	3	9
β-strand	933-935	3	9
β-strand	942-944	3	9
α-helix	954-957	4
α-helix	966-981	16
α-helix	984-987	4
α-helix	995-1016	22
α-helix	1025-1027	3
α-helix	1029-1051	23
β-strand	1058	1	10
β-strand	1062	1	10
α-helix	1067-1082	16
α-helix	1086-1089	4
α-helix	1095-1105	11
α-helix	1110-1118	9

Chain L: 5 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	19-22	4
α-helix	28-34	7
β-strand	43-44	2	12
α-helix	47-73	27
β-strand	79	1	11
α-helix	82-90	9
α-helix	93-97	5

Chain M: 4 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	39-49	11
β-strand	55	1	11
α-helix	57-85	29
β-strand	89-90	2	12
α-helix	92-102	11
α-helix	106-123	18

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Telomerase reverse transcriptase,Telomerase reverse transcriptase	A	protein	1332	Homo sapiens	O14746 (AlphaFold model)
DNA (5'-d(ptptpapgpgpg)-3')	N	DNA	18	synthetic construct
Histone H2B	M	protein	166	Homo sapiens	B4DR52 (AlphaFold model)
Histone H2A	L	protein	130	Homo sapiens	P0C0S8 (AlphaFold model)
RNA (256-mer)	B	RNA	451	Homo sapiens

Sequence of entity 1 (A), FASTA

>7BG9_1 Telomerase reverse transcriptase,Telomerase reverse transcriptase (chains A)
MKTAALAQHDEAVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLAEA
KKLNDAQAPKVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLAEAKK
LNGAQAPKVDANSAGKSTDYDIPTTASENLYFQGHKLGTFQGPWSHPQFEKGSAGSAAGS
GAGWSHPQFEKSRPTTASGTMPRAPRCRAVRSLLRSHYREVLPLATFVRRLGPQGWRLVQ
RGDPAAFRALVAQCLVCVPWDARPPPAAPSFRQVSCLKELVARVLQRLCERGAKNVLAFG
FALLDGARGGPPEAFTTSVRSYLPNTVTDALRGSGAWGLLLRRVGDDVLVHLLARCALFV
LVAPSCAYQVCGPPLYQLGAATQARPPPHASGPRRRLGCERAWNHSVREAGVPLGLPAPG
ARRRGGSASRSLPLPKRPRRGAAPEPERTPVGQGSWAHPGRTRGPSDRGFCVVSPARPAE
EATSLEGALSGTRHSHPSVGRQHHAGPPSTSRPPRPWDTPCPPVYAETKHFLYSSGDKEQ
LRPSFLLSSLRPSLTGARRLVETIFLGSRPWMPGTPRRLPRLPQRYWQMRPLFLELLGNH
AQCPYGVLLKTHCPLRAAVTPAAGVCAREKPQGSVAAPEEEDTDPRRLVQLLRQHSSPWQ
VYGFVRACLRRLVPPGLWGSRHNERRFLRNTKKFISLGKHAKLSLQELTWKMSVRDCAWL
RRSPGVGCVPAAEHRLREEILAKFLHWLMSVYVVELLRSFFYVTETTFQKNRLFFYRKSV
WSKLQSIGIRQHLKRVQLRELSEAEVRQHREARPALLTSRLRFIPKPDGLRPIVNMDYVV
GARTFRREKRAERLTSRVKALFSVLNYERARRPGLLGASVLGLDDIHRAWRTFVLRVRAQ
DPPPELYFVKVDVTGAYDTIPQDRLTEVIASIIKPQNTYCVRRYAVVQKAAHGHVRKAFK
SHVSTLTDLQPYMRQFVAHLQETSPLRDAVVIEQSSSLNEASSGLFDVFLRFMCHHAVRI
RGKSYVQCQGIPQGSILSTLLCSLCYGDMENKLFAGIRRDGLLLRLVDDFLLVTPHLTHA
KTFLRTLVRGVPEYGCVVNLRKTVVNFPVEDEALGGTAFVQMPAHGLFPWCGLLLDTRTL
EVQSDYSSYARTSIRASLTFNRGFKAGRNMRRKLFGVLRLKCHSLFLDLQVNSLQTVCTN
IYKILLLQAYRFHACVLQLPFHQQVWKNPTFFLRVISDTASLCYSILKAKNAGMSLGAKG
AAGPLPSEAVQWLCHQAFLLKLTRHRVTYVPLLGSLRTAQTQLSRKLPGTTLTALEAAAN
PALPSDFKTILD

Sequence of entity 2 (N), FASTA

>7BG9_2 DNA (5'-D(P*TP*TP*AP*GP*GP*G)-3') (chains N)
TTTTTTTTTTTTTTAGGG

Sequence of entity 3 (M), FASTA

>7BG9_3 Histone H2B (chains M)
MPDPAKSAPAPKKGSKKAVTKVQKKDGKKRKRSRKESYSVYVYKVLKQVHPDTGISSKAM
GIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVT
KYTSSNPRNLSPTKPGGSEDRQPPPSQLSAIPPFCLVLRAGIAGQV

Sequence of entity 4 (L), FASTA

>7BG9_4 Histone H2A (chains L)
MSGRGKQGGKARAKAKTRSSRAGLQFPVGRVRRLLRKGNYAERVGAGAPVYLAAVLEYLT
AEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGKVTIAQGGVLPNIQAVLLPKK
TESHHKAKGK

Sequence of entity 5 (B), FASTA

>7BG9_5 RNA (256-MER) (chains B)
GGGUUGCGGAGGGUGGGCCUGGGAGGGGUGGUGGCCAUUUUUUGUCUAACCCUAACUGAG
AAGGGCGUAGGCGCCGUGCUUUUGCUCCCCGCGCGCUGUUUUUCUCGCUGACUUUCAGCG
GGCGGAAAAGCCUCGGCCUGCCGCCUUCCACCGUUCAUUCUAGAGCAAACAAAAAAUGUC
AGCUGCUGGCCCGUUCGCCCCUCCCGGGGACCUGCGGCGGGUCGCCUGCCCAGCCCCCGA
ACCCCGCCUGGAGGCCGCGGUCGGCCCGGGGCUUCUCCGGAGGCACCCACUGCCACCGCG
AAGAGUUGGGCUCUGUCAGCCGCGGGUCUCUCGGGGGCGAGGGCGAGGUUCAGGCCUUUC
AGGCCGCAGGAAGAGGAACGGAGCGAGUCCCCGCGCGCGGCGCGAUUCCCUGAGCUGUGG
GACGUGCACCCAGGACUCGGCUCACACAUGC

Primary citation

Structure of human telomerase holoenzyme with bound telomeric DNA. Ghanim, G.E., Fountain, A.J., van Roon, A.M. et al. Nature (2021) 593:449-453. DOI 10.1038/s41586-021-03415-4 · PubMed

Other PDB entries of the same protein (UniProt O14746 (AlphaFold model), which also has an AlphaFold model), best resolution first:

5MEO 1.77 Å, Human Leukocyte Antigen presenting ILGKFLHRL
5MER 1.88 Å, Human Leukocyte Antigen A02 presenting ILAKFLHEL
5MEQ 2.27 Å, Human Leukocyte Antigen A02 presenting ILAKFLHTL
5UGW 2.31 Å, Structure of the human telomerase thumb domain
4B18 2.52 Å, The crystal structure of human Importin alpha 5 with TERT NLS peptide
9Q16 2.7 Å, Human telomerase catalytic core with shelterin protein TPP1, dGpNHpp and DNA primer…
5MEP 2.71 Å, Human Leukocyte Antigen A02 presenting ILGKFLHWL
4MNQ 2.74 Å, TCR-peptide specificity overrides affinity enhancing TCR-MHC interactions
2BCK 2.8 Å, Crystal Structure of HLA-A*2402 Complexed with a telomerase peptide
9Q12 2.8 Å, Human telomerase catalytic core with shelterin protein TPP1 and DNA primer ending in TTAG
5MEN 2.81 Å, Human Leukocyte Antigen A02 presenting ILAKFLHWL, in complex with cognate T-Cell Receptor
9Q10 2.9 Å, Human telomerase catalytic core with shelterin protein TPP1, dTpNHpp and DNA primer…

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