7BLP: Vacuolar protein sorting-associated protein 35

Vps35/Vps29 arch of fungal membrane-assembled retromer:Grd19 complex. Determined by electron microscopy at 9.5 Å resolution. Released 10 Feb 2021.

Method
Electron microscopy
Resolution
9.5 Å
Organism
Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)
Chains
4
Atoms
14,950
Mol. weight
241.79 kDa
Released
10 Feb 2021

Explore 7BLP in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7BLP contains 95 α-helices and 31 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 43 helices, 0 β-strands

ElementResiduesLengthSheet
α-helix13-3321
α-helix38-4912
α-helix50-534
α-helix59-8628
α-helix92-954
α-helix96-983
α-helix102-11918
α-helix124-13411
α-helix135-1373
α-helix141-15515
α-helix171-19424
α-helix198-2003
α-helix201-22222
α-helix228-2314
α-helix232-2365
α-helix237-24610
α-helix250-26213
α-helix265-2695
α-helix272-2798
α-helix288-30417
α-helix389-40315
α-helix408-42518
α-helix430-44718
α-helix457-47216
α-helix477-4815
α-helix483-4908
α-helix494-51017
α-helix518-53215
α-helix561-57212
α-helix577-59014
α-helix598-61720
α-helix625-64521
α-helix653-67018
α-helix675-69016
α-helix695-70915
α-helix717-73115
α-helix737-75115
α-helix756-7583
α-helix771-78616
α-helix791-81020
α-helix818-83215
α-helix836-8383
α-helix840-85617
Chain B: 3 helices, 15 β-strands
ElementResiduesLengthSheet
β-strand5-841
α-helix22-276
β-strand3012
β-strand3312
β-strand35-3841
α-helix45-5410
β-strand57-6041
β-strand76-8053
β-strand83-8753
α-helix98-10811
β-strand112-11433
β-strand122-12763
β-strand130-13563
β-strand13714
β-strand15514
β-strand156-16381
β-strand166-175101
β-strand183-192101
Chain C: 46 helices, 2 β-strands
ElementResiduesLengthSheet
α-helix13-3321
α-helix38-4912
α-helix50-534
α-helix59-8628
α-helix92-954
α-helix96-983
α-helix102-11918
α-helix124-13411
α-helix141-15414
α-helix171-19424
α-helix198-2003
α-helix201-22323
α-helix228-2314
α-helix232-2365
α-helix237-24610
α-helix250-26213
α-helix265-2695
α-helix272-2798
α-helix288-30417
α-helix389-40315
α-helix408-42518
α-helix430-4356
α-helix436-4427
α-helix443-4475
α-helix450-4512
α-helix457-47216
α-helix476-4794
α-helix483-4853
α-helix487-4915
α-helix494-51017
α-helix512-5132
α-helix518-53215
α-helix562-57211
α-helix577-58913
α-helix598-61720
α-helix626-64621
α-helix653-67018
α-helix675-69117
α-helix695-70915
α-helix717-73115
α-helix737-75115
β-strand75215
β-strand75415
α-helix756-7583
α-helix771-78616
α-helix792-81019
α-helix818-83417
α-helix840-85617
Chain D: 3 helices, 14 β-strands
ElementResiduesLengthSheet
β-strand4-746
β-strand1317
α-helix22-276
β-strand35-3846
β-strand4317
α-helix45-5410
β-strand57-6046
β-strand79-8028
β-strand83-8428
β-strand85-8739
α-helix99-10810
β-strand112-11439
β-strand123-12759
β-strand130-13459
β-strand156-16386
β-strand166-176116
β-strand182-192116

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Vacuolar protein sorting-associated protein 35A, Cprotein869Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)G0S709 (AlphaFold model)
Vacuolar protein sorting-associated protein 29B, Dprotein202Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)G0RZB5 (AlphaFold model)
Sequence of entity 1 (A, C), FASTA
>7BLP_1 Vacuolar protein sorting-associated protein 35 (chains A, C)
MSTPAPPEEQARLLEDALIAVRQQTAMMRKFLDTPGKLMDALKCCSTLVSELRTSSLSPK
QYYELYMAVFDALRYLSAHLRENHPVNHLADLYELVQYAGNIIPRLYLMITVGTAYMSID
GAPVKELMKDMMDMSRGVQHPVRGLFLRYYLSGQARDYLPTGDSDGPEGNLQDSINFILT
NFVEMNKLWVRLQHQGHSRERDLRTQERRELQLLVGSNIVRLSQLVDLPTYRDSILGPLL
EQIVQCRDILAQEYLLEVITQVFPDEYHLHTLDQFLGAVSRLNPHVNVKAIVIGMMNRLS
DYAERESQNEPEEDRAKLEEEALAKLLEKTKLGQNSELEPQNGDHPDTEVSSTTDSAQAP
STADSDTTAVNGEEEPVRKRRGIPVNVPLYDIFFDQVQHLVQAQHLPIQDTIALCCSLAN
LSLNIYPERLDYVDGILAYALAKVKEHANSADLHSQPAQQSLLSLLQSPLRRYVSIFTAL
SLPTYVSLFQAQTYPTRRAIAGEIVRTLLKNQTLISTPAHLENVLEILKVLIKEGSQPPA
GYPGVVQPRARPLETDETMEEQGWLARLVHLIHSDDNDTQFRLLQMTRKAYAEGNERIRT
TTPPLITAGLKLARRFKAREHYDDNWSSQSSSLFKFLHSAISTLYTRVNGPGVADLCLRL
FCSCGQVADMTEFEEVAYEFFAQAFTVYEESISDSKAQFQAVCVIASALHRTRNFGRENY
DTLITKCAQHASKLLRKPDQCRAVYLASHLWWATPIAARGETEDTELYRDGKRVLECLQR
ALRVADSCMETATSIELFVEILDRYVYYFDQRNESVTTKYLNGLIELIHSNLAGNQQDSA
SVEASRKHFIQTLEMIQSKEFEGIVVAPK
Sequence of entity 2 (B, D), FASTA
>7BLP_2 Vacuolar protein sorting-associated protein 29 (chains B, D)
SMAFLILVIGNLHIPDRALDIPPKFKKLLSPGKISQTLCLGNLTDRATYDYLRSISPDLK
IVRGRMDVEATSLPLMQVVTHGSLRIGFLEGFTLVSEEPDVLLAEANKLDVDVLCWAGGS
HRFECFEYMDKFFVNPGSATGAFTTDWLAEGEEVVPSFCLMDVQGISLTLYVYQLRKDEN
GTENVAVEKVTYTKPVEPTGAS

Primary citation

Architecture and mechanism of metazoan retromer:SNX3 tubular coat assembly. Leneva, N., Kovtun, O., Morado, D.R. et al. Sci Adv (2021) 7. DOI 10.1126/sciadv.abf8598 · PubMed

Other PDB entries of the same protein (UniProt G0S709 (AlphaFold model), which also has an AlphaFold model), best resolution first:

Browse structure collections

About this viewer

MolViewer shows 7BLP directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.