7CKY: PDB entry 7CKY
Cryo-EM structure of PW0464 bound dopamine receptor DRD1-Gs signaling complex. Determined by electron microscopy at 3.2 Å resolution. Released 3 Mar 2021.
- Method
- Electron microscopy
- Resolution
- 3.2 Å
- Organisms
- Homo sapiens, Lama glama
- Chains
- 5
- Atoms
- 8,128
- Mol. weight
- 158.93 kDa
- Ligands
- G3U, CLR
- Released
- 3 Mar 2021
Explore 7CKY in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
7CKY contains 32 α-helices and 47 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain A: 10 helices, 7 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 15-39 | 25 | |
| β-strand | 41-47 | 7 | 1 |
| α-helix | 53-58 | 6 | |
| β-strand | 210-214 | 5 | 1 |
| β-strand | 217-223 | 7 | 1 |
| α-helix | 234-237 | 4 | |
| β-strand | 243-249 | 7 | 1 |
| α-helix | 265-277 | 13 | |
| α-helix | 282-284 | 3 | |
| β-strand | 286-292 | 7 | 1 |
| α-helix | 294-303 | 10 | |
| α-helix | 313-315 | 3 | |
| α-helix | 326-327 | 2 | |
| α-helix | 332-350 | 19 | |
| β-strand | 359 | 1 | 1 |
| β-strand | 362-363 | 2 | 1 |
| α-helix | 371-390 | 20 | |
Chain B: 2 helices, 30 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 3-24 | 22 | |
| α-helix | 30-33 | 4 | |
| β-strand | 47-51 | 5 | 2 |
| β-strand | 58-62 | 5 | 3 |
| β-strand | 69-74 | 6 | 3 |
| β-strand | 78-83 | 6 | 3 |
| β-strand | 88-94 | 7 | 3 |
| β-strand | 100 | 1 | 4 |
| β-strand | 104-105 | 2 | 5 |
| β-strand | 111-113 | 3 | 5 |
| β-strand | 116 | 1 | 4 |
| β-strand | 121-125 | 5 | 5 |
| β-strand | 134-139 | 6 | 5 |
| β-strand | 146-151 | 6 | 6 |
| β-strand | 157-161 | 5 | 6 |
| β-strand | 165-168 | 4 | 6 |
| β-strand | 178-181 | 4 | 6 |
| β-strand | 187-192 | 6 | 7 |
| β-strand | 198-203 | 6 | 7 |
| β-strand | 208-212 | 5 | 7 |
| β-strand | 218-222 | 5 | 7 |
| β-strand | 229-234 | 6 | 8 |
| β-strand | 240-245 | 6 | 8 |
| β-strand | 250-254 | 5 | 8 |
| β-strand | 259-264 | 6 | 8 |
| β-strand | 273-278 | 6 | 9 |
| β-strand | 284-289 | 6 | 9 |
| β-strand | 294-298 | 5 | 9 |
| β-strand | 303-307 | 5 | 9 |
| β-strand | 317-320 | 4 | 2 |
| β-strand | 327-330 | 4 | 2 |
| β-strand | 336-339 | 4 | 2 |
Chain G: 3 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 6-23 | 18 | |
| α-helix | 30-44 | 15 | |
| α-helix | 45-47 | 3 | |
Chain N: 2 helices, 10 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 3-8 | 6 | 10 |
| β-strand | 10-12 | 3 | 11 |
| β-strand | 17-25 | 9 | 10 |
| α-helix | 29-31 | 3 | |
| β-strand | 35-39 | 5 | 11 |
| β-strand | 45-50 | 6 | 11 |
| β-strand | 59-60 | 2 | 11 |
| β-strand | 68-73 | 6 | 10 |
| β-strand | 78-84 | 7 | 10 |
| α-helix | 88-90 | 3 | |
| β-strand | 92-95 | 4 | 11 |
| β-strand | 122-126 | 5 | 11 |
Chain R: 15 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 24-50 | 27 | |
| α-helix | 59-76 | 18 | |
| α-helix | 78-87 | 10 | |
| α-helix | 93-95 | 3 | |
| α-helix | 96-126 | 31 | |
| α-helix | 128-134 | 7 | |
| α-helix | 137-160 | 24 | |
| α-helix | 192-199 | 8 | |
| α-helix | 200-204 | 5 | |
| α-helix | 205-236 | 32 | |
| α-helix | 264-274 | 11 | |
| α-helix | 275-279 | 5 | |
| α-helix | 280-294 | 15 | |
| α-helix | 310-331 | 22 | |
| α-helix | 335-343 | 9 | |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | A | protein | 394 | Homo sapiens | P63092 (AlphaFold model) |
| Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | B | protein | 348 | Homo sapiens | P62873 (AlphaFold model) |
| Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | G | protein | 71 | Homo sapiens | P59768 (AlphaFold model) |
| Nanobody 35 | N | protein | 156 | Lama glama | |
| D(1A) dopamine receptor | R | protein | 446 | Homo sapiens | P21728 (AlphaFold model) |
Sequence of entity 1 (A), FASTA
>7CKY_1 Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKSTIVKQM
RILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELA
NPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLD
KIDVIKQADYVPSDQDLLRCRVLTTGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFND
VTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEK
VLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCY
PHFTCSVDTENIRRVFNDCRDIIQRMHLRQYELL
Sequence of entity 2 (B), FASTA
>7CKY_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLR
GHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYV
ACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIE
TGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAI
CFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDF
NCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN
Sequence of entity 3 (G), FASTA
>7CKY_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL
Sequence of entity 4 (N), FASTA
>7CKY_4 Nanobody 35 (chains N)
MKYLLPTAAAGLLLLAAQPAMAQVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVR
QAPGKGLEWVSDISQSGASISYTGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCAR
CPAPFTRDCFDVTSTTYAYRGQGTQVTVSSHHHHHH
Sequence of entity 5 (R), FASTA
>7CKY_5 D(1A) dopamine receptor (chains R)
MRTLNTSAMDGTGLVVERDFSVRILTACFLSLLILSTLLGNTLVCAAVIRFRHLRSKVTN
FFVISLAVSDLLVAVLVMPWKAVAEIAGFWPFGSFCNIWVAFDIMCSTASILNLCVISVD
RYWAISSPFRYERKMTPKAAFILISVAWTLSVLISFIPVQLSWHKAKPTSPSDGNATSLA
ETIDNCDSSLSRTYAISSSVISFYIPVAIMIVTYTRIYRIAQKQIRRIAALERAAVHAKN
CQTTTGNGKPVECSQPESSFKMSFKRETKVLKTLSVIMGVFVCCWLPFFILNCILPFCGS
GETQPFCIDSNTFDVFVWFGWANSSLNPIIYAFNADFRKAFSTLLGCYRLCPATNNAIET
VSINNNGAAMFSSHHEPRGSISKECNLVYLIPHAVGSSEDLKKEEAAGIARPLEKLSPAL
SVILDYDTDVSLEKIQPITQNGQHPT
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| G3U | 6-[4-[3-[bis(fluoranyl)methoxy]pyridin-2-yl]oxy-2-methyl-phenyl]-1,5-dimethyl-p… | C19 H17 F2 N3 O4 | 1 |
| CLR | Cholesterol | C27 H46 O | 1 |
Primary citation
Ligand recognition and allosteric regulation of DRD1-Gs signaling complexes. Xiao, P., Yan, W., Gou, L. et al. Cell (2021) 184:943-956.e18. DOI 10.1016/j.cell.2021.01.028 · PubMed
Other PDB entries of the same protein (UniProt P63092 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 7BPH 1.57 Å, Crystal structure of GppNHp-bound GNAS in complex with the cyclic peptide inhibitor GN13
- 6AU6 1.7 Å, Crystal structure of GDP-bound human GNAS R201C mutant
- 8F0K 1.9 Å, Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385
- 7E5E 1.95 Å, Crystal structure of GDP-bound GNAS in complex with the cyclic peptide inhibitor GD20
- 7MBX 1.95 Å, Human Cholecystokinin 1 receptor (CCK1R) Gs complex
- 8F0J 2.0 Å, Calcitonin Receptor in complex with Gs and Pramlintide analogue peptide San45
- 8F2B 2.0 Å, Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
- 9XXT 2.0 Å, Cryo-EM structure of lysophosphatidylserine (18:0)-bound GPR174-Gs complex
- 6X18 2.1 Å, GLP-1 peptide hormone bound to Glucagon-Like peptide-1 (GLP-1) Receptor
- 6X19 2.1 Å, Non peptide agonist CHU-128, bound to Glucagon-Like peptide-1 (GLP-1) Receptor
- 9NTU 2.1 Å, Cryo-EM structure of BETP-GLP-1(9-36)-GLP-1R-Gs complex
- 7RTB 2.14 Å, Peptide-19 bound to the Glucagon-Like Peptide-1 Receptor (GLP-1R)
Browse structure collections
About this viewer
MolViewer shows 7CKY directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.