7DFC: Crystal of Arrestin2-V2Rpp-3-Fab30 complex

Crystal of Arrestin2-V2Rpp-3-Fab30 complex. Determined by X-ray diffraction at 2.49 Å resolution. Released 28 Jul 2021.

Method: X-ray diffraction
Resolution: 2.49 Å
Organisms: Bos taurus, synthetic construct, Mus musculus
Chains: 4
Atoms: 5,978
Mol. weight: 102.83 kDa
Released: 28 Jul 2021

Explore 7DFC in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7DFC contains 25 α-helices and 70 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 15 helices, 26 β-strands

Element	Residues	Length	Sheet
β-strand	7-12	6	1
β-strand	18-22	5	1
β-strand	26-28	3	2
β-strand	29	1	3
β-strand	34	1	3
β-strand	37-43	7	1
β-strand	53-63	11	4
β-strand	73-87	15	4
α-helix	90	1
α-helix	96-98	3
α-helix	99-108	10
β-strand	112-117	6	1
α-helix	118-120	3
α-helix	124-126	3
β-strand	127-129	3	4
α-helix	130-131	2
α-helix	139-140	2
β-strand	141-150	10	4
β-strand	163-168	6	4
β-strand	169-171	3	2
β-strand	183-188	6	5
α-helix	195-196	2
β-strand	197-203	7	5
β-strand	207-209	3	6
β-strand	214-222	9	5
β-strand	228-243	16	7
β-strand	245-258	14	7
β-strand	262	1	7
β-strand	266-274	9	5
α-helix	279-281	3
β-strand	288-290	3	4
α-helix	291-292	2
α-helix	299	1
β-strand	300	1	4
α-helix	301-304	4
β-strand	316-329	14	7
α-helix	333-335	3
β-strand	343-349	7	7
β-strand	350-352	3	6
α-helix	353-355	3
α-helix	359-361	3

Chain H: 4 helices, 21 β-strands

Element	Residues	Length	Sheet
β-strand	18-21	4	13
β-strand	26-27	2	14
β-strand	32-40	9	13
α-helix	44-46	3
β-strand	47-54	8	15
β-strand	61-67	7	15
β-strand	72-75	4	15
β-strand	83-88	6	13
β-strand	93-99	7	13
β-strand	107-115	9	15
β-strand	122-125	4	15
β-strand	129-131	3	15
β-strand	132-133	2	14
β-strand	142-146	5	16
α-helix	150-152	3
β-strand	153-154	2	16
β-strand	157-167	11	16
β-strand	173-176	4	17
β-strand	185-187	3	16
α-helix	188-190	3
β-strand	191-192	2	16
β-strand	198-207	10	16
β-strand	217-222	6	17
α-helix	223-225	3
β-strand	227-232	6	17

Chain L: 5 helices, 21 β-strands

Element	Residues	Length	Sheet
β-strand	17-19	3	8
β-strand	23-25	3	9
β-strand	32-38	7	8
β-strand	46-51	6	9
β-strand	58-62	5	9
β-strand	66-67	2	9
α-helix	68	1
β-strand	75-80	6	8
β-strand	83-88	6	8
β-strand	98-103	6	9
α-helix	109	1
β-strand	110-111	2	9
β-strand	116-118	3	9
β-strand	124	1	10
β-strand	127-131	5	11
α-helix	132-134	3
α-helix	135-138	4
β-strand	142-152	11	11
β-strand	153	1	10
β-strand	158-163	6	12
β-strand	167	1	12
β-strand	172-176	5	11
β-strand	186-195	10	11
α-helix	196-201	6
β-strand	205-211	7	12
β-strand	214-222	9	12

Chain V: 1 helix, 2 β-strands

Element	Residues	Length	Sheet
β-strand	349-351	3	4
α-helix	358-360	3
β-strand	361-364	4	1

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Beta-arrestin-1	A	protein	426	Bos taurus	P17870 (AlphaFold model)
V2Rpp-3	V	protein	22	synthetic construct	P30518 (AlphaFold model)
FAB30 light chain	L	protein	227	Mus musculus
FAB30 heavy chain	H	protein	249	Mus musculus

Sequence of entity 1 (A), FASTA

>7DFC_1 Beta-arrestin-1 (chains A)
MGDKGTRVFKKASPNGKLTVYLGKRDFVDHIDLVEPVDGVVLVDPEYLKERRVYVTLTCA
FRYGREDLDVLGLTFRKDLFVANVQSFPPAPEDKKPLTRLQERLIKKLGEHAYPFTFEIP
PNLPCSVTLQPGPEDTGKACGVDYEVKAFCAENLEEKIHKRNSVRLVIRKVQYAPERPGP
QPTAETTRQFLMSDKPLHLEASLDKEIYYHGEPISVNVHVTNNTNKTVKKIKISVRQYAD
ICLFNTAQYKCPVAMEEADDTVAPSSTFCKVYTLTPFLANNREKRGLALDGKLKHEDTNL
ASSTLLREGANREILGIIVSYKVKVKLVVSRGGLLGDLASSDVAVELPFTLMHPKPKEEP
PHREVPEHETPVDTNLIELDTNDDDIVFEDFARQRLKGMKDDKEEEEDGTGSPRLNDRLE
HHHHHH

Sequence of entity 2 (V), FASTA

>7DFC_2 V2Rpp-3 (chains V)
RTPPSLGPQDESCTTASSSLRK

Sequence of entity 3 (L), FASTA

>7DFC_3 FAB30 LIGHT CHAIN (chains L)
MFVFSIATNAYASDIQMTQSPSSLSASVGDRVTITCRASQSVSSAVAWYQQKPGKAPKLL
IYSASSLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQYKYVPVTFGQGTKVEIK
RTVAAPSVFIFPPSDSQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQD
SKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC

Sequence of entity 4 (H), FASTA

>7DFC_4 FAB30 HEAVY CHAIN (chains H)
MFVFSIATNAYAEISEVQLVESGGGLVQPGGSLRLSCAASGFNVYSSSIHWVRQAPGKGL
EWVASISSYYGYTYYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARSRQFWYS
GLDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGA
LTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDK
THHHHHHHH

Primary citation

Structural studies of phosphorylation-dependent interactions between the V2R receptor and arrestin-2. He, Q.T., Xiao, P., Huang, S.M. et al. Nat Commun (2021) 12:2396-2396. DOI 10.1038/s41467-021-22731-x · PubMed

Other PDB entries of the same protein (UniProt P17870 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1G4M 1.9 Å, Crystal structure of bovine beta-arrestin 1
1G4R 2.2 Å, Crystal structure of bovine beta-arrestin 1
3GC3 2.2 Å, Crystal Structure of Arrestin2S and Clathrin
7DFA 2.54 Å, Crystal of Arrestin2-V2Rpp-4-Fab30 complex
22GM 2.7 Å, C5a bound C5aR1 in complex with beta-arrestin1
9WSV 2.8 Å, Cryo-EM structure of DAMGO-muOR-arrestin-1-Fab30 complex
9WSX 2.8 Å, Cryo-EM structure of endomorphin-1-muOR-arrestin2-Fab30 complex
22GK 2.84 Å, C5a bound C5aR2 in complex with beta-arrestin1
1JSY 2.9 Å, Crystal structure of bovine arrestin-2
1ZSH 2.9 Å, Crystal structure of bovine arrestin-2 in complex with inositol hexakisphosphate (IP6)
22CR 2.9 Å, C5a-desArg bound C5aR2 in complex with beta-arrestin1
2WTR 2.9 Å, Full length Arrestin2

Browse structure collections

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