Structure of a glucose isomerase crystal grown in an aqueous glycerol solution without any precipitants. Determined by X-ray diffraction at 0.99 Å resolution. Released 8 Dec 2021.
Explore 7DMM in 3D Show helices and sheets RCSB PDB PDBe
7DMM contains 22 α-helices and 18 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 7-9 | 3 | |
| β-strand | 11-14 | 4 | 1 |
| α-helix | 15-18 | 4 | |
| β-strand | 24 | 1 | 2 |
| β-strand | 27 | 1 | 2 |
| α-helix | 32-35 | 4 | |
| α-helix | 36-45 | 10 | |
| β-strand | 50-54 | 5 | 1 |
| α-helix | 55-58 | 4 | |
| α-helix | 65-82 | 18 | |
| β-strand | 85-90 | 6 | 1 |
| α-helix | 97-99 | 3 | |
| α-helix | 109-128 | 20 | |
| β-strand | 133-136 | 4 | 1 |
| β-strand | 142-143 | 2 | 3 |
| α-helix | 151-172 | 22 | |
| β-strand | 177-180 | 4 | 1 |
| β-strand | 190-191 | 2 | 3 |
| α-helix | 196-203 | 8 | |
| α-helix | 209-211 | 3 | |
| β-strand | 212-214 | 3 | 1 |
| β-strand | 217 | 1 | 1 |
| α-helix | 218-222 | 5 | |
| α-helix | 228-237 | 10 | |
| β-strand | 241 | 1 | 1 |
| β-strand | 245-246 | 2 | 1 |
| β-strand | 248 | 1 | 4 |
| β-strand | 258 | 1 | 4 |
| α-helix | 259 | 1 | |
| β-strand | 260 | 1 | 5 |
| β-strand | 262 | 1 | 5 |
| α-helix | 265-278 | 14 | |
| β-strand | 284-286 | 3 | 1 |
| α-helix | 296-322 | 27 | |
| α-helix | 324-332 | 9 | |
| α-helix | 335-338 | 4 | |
| α-helix | 347-352 | 6 | |
| α-helix | 354-356 | 3 | |
| α-helix | 362-367 | 6 | |
| α-helix | 372-384 | 13 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| Xylose isomerase | A | protein | 386 | Streptomyces rubiginosus | P24300 (AlphaFold model) |
>7DMM_1 Xylose isomerase (chains A) NYQPTPEDRFTFGLWTVGWQGRDPFGDATRRALDPVESVQRLAELGAHGVTFHDDDLIPF GSSDSEREEHVKRFRQALDDTGMKVPMATTNLFTHPVFKDGGFTANDRDVRRYALRKTIR NIDLAVELGAETYVAWGGREGAESGGAKDVRDALDRMKEAFDLLGEYVTSQGYDIRFAIE PKPNEPRGDILLPTVGHALAFIERLERPELYGVNPEVGHEQMAGLNFPHGIAQALWAGKL FHIDLNGQNGIKYDQDLRFGAGDLRAAFWLVDLLESAGYSGPRHFDFKPPRTEDFDGVWA SAAGCMRNYLILKERAAAFRADPEVQEALRASRLDELARPTAADGLQALLDDRSAFEEFD VDAAAARGMAFERLDQLAMDHLLGAR
Water and common crystallization additives (GOL) are not listed.
Extraordinarily fast growth of high-quality glucose isomerase crystals simply by concentration in a precipitant-free solution with a cryoprotectant. Suzuki, Y., Ikemitsu, N., Maita, N. To be published.
Other PDB entries of the same protein (UniProt P24300 (AlphaFold model), which also has an AlphaFold model), best resolution first:
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