7DSX: Calcineurin B homologous protein 1

Structure of a human NHE1-CHP1 complex under pH 7.5, bound by cariporide. Determined by electron microscopy at 3.5 Å resolution. Released 23 Jun 2021.

Method
Electron microscopy
Resolution
3.5 Å
Organism
Homo sapiens
Chains
4
Atoms
11,106
Mol. weight
162.01 kDa
Ligands
HG0, PGT
Released
23 Jun 2021

Explore 7DSX in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7DSX contains 84 α-helices and 8 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 29 helices, 2 β-strands

ElementResiduesLengthSheet
α-helix100-12122
α-helix125-1284
α-helix133-14614
α-helix166-17510
α-helix179-1846
α-helix186-1894
α-helix194-1974
α-helix200-21314
α-helix224-23411
β-strand23714
α-helix241-2466
α-helix253-26614
α-helix267-2693
α-helix289-29911
α-helix300-3045
α-helix305-3117
α-helix327-3293
α-helix330-3334
α-helix352-3587
α-helix364-3674
α-helix374-40027
α-helix411-43626
α-helix446-4549
β-strand45814
α-helix460-4634
α-helix478-48912
α-helix490-4945
α-helix499-5068
α-helix519-53820
α-helix543-55614
α-helix572-59019
Chain B: 29 helices, 2 β-strands
ElementResiduesLengthSheet
α-helix100-12122
α-helix125-1284
α-helix133-14614
α-helix166-17510
α-helix179-1846
α-helix186-1894
α-helix194-1974
α-helix200-21314
α-helix223-23412
β-strand23712
α-helix241-2466
α-helix253-26614
α-helix267-2693
α-helix289-29911
α-helix300-3045
α-helix305-3117
α-helix327-3293
α-helix330-3334
α-helix352-3587
α-helix364-3674
α-helix374-40027
α-helix411-43626
α-helix446-4549
β-strand45812
α-helix460-4634
α-helix478-48912
α-helix490-4945
α-helix499-5068
α-helix519-53820
α-helix543-55614
α-helix572-59019
Chains C and D: 13 helices, 2 β-strands
ElementResiduesLengthSheet
α-helix15-217
α-helix25-3915
β-strand4613
α-helix48-525
α-helix63-708
β-strand7813
α-helix80-878
α-helix88-903
α-helix92-932
α-helix95-995
α-helix111-12212
α-helix132-14211
α-helix149-16315
α-helix174-1807
α-helix185-1884

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Calcineurin B homologous protein 1C, Dprotein185Homo sapiensQ99653 (AlphaFold model)
Sodium/hydrogen exchanger 1A, Bprotein509Homo sapiensP19634 (AlphaFold model)
Sequence of entity 1 (C, D), FASTA
>7DSX_1 Calcineurin B homologous protein 1 (chains C, D)
DEELEEIKKETGFSHSQITRLYSRFTSLDKGENGTLSREDFQRIPELAINPLGDRIINAF
FPEGEDQVNFRGFMRTLAHFRPIEDNEKSKDVNGPEPLNSRSNKLHFAFRLYDLDKDEKI
SRDELLQVLRMMVGVNISDEQLGSIADRTIQEADQDGDSAASFTEFVKVLEKVDVEQKMS
IRFLH
Sequence of entity 2 (A, B), FASTA
>7DSX_2 Sodium/hydrogen exchanger 1 (chains A, B)
PRKAFPVLGIDYTHVRTPFEISLWILLACLMKIGFHVIPTISSIVPESCLLIVVGLLVGG
LIKGVGETPPFLQSDVFFLFLLPPIILDAGYFLPLRQFTENLGTILIFAVVGTLWNAFFL
GGLMYAVCLVGGEQINNIGLLDNLLFGSIISAVDPVAVLAVFEEIHINELLHILVFGESL
LNDAVTVVLYHLFEEFANYEHVGIVDIFLGFLSFFVVALGGVLVGVVYGVIAAFTSRFTS
HIRVIEPLFVFLYSYMAYLSAELFHLSGIMALIASGVVMRPYVEANISHKSHTTIKYFLK
MWSSVSETLIFIFLGVSTVAGSHHWNWTFVISTLLFCLIARVLGVLGLTWFINKFRIVKL
TPKDQFIIAYGGLRGAIAFSLGYLLDKKHFPMCDLFLTAIITVIFFTVFVQGMTIRPLVD
LLAVKKKQETKRSINEEIHTQFLDHLLTGIEDICGHYGHHHWKDKLNRFNKKYVKKCLIA
GERSKEPQLIAFYHKMEMKQAIELVESGG

Ligands and cofactors

IDNameFormulaCopies
HG0N-[bis(azanyl)methylidene]-3-methylsulfonyl-4-propan-2-yl-benzamideC12 H17 N3 O3 S2
PGT(1S)-2-{[{[(2R)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(palmitoylo…C40 H79 O10 P6

Primary citation

Structure and mechanism of the human NHE1-CHP1 complex. Dong, Y., Gao, Y., Ilie, A. et al. Nat Commun (2021) 12:3474-3474. DOI 10.1038/s41467-021-23496-z · PubMed

Other PDB entries of the same protein (UniProt Q99653 (AlphaFold model), which also has an AlphaFold model), best resolution first:

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