Cryo-EM structure of hDisp1NNN-ShhN. Determined by electron microscopy at 4.07 Å resolution. Released 8 Dec 2021.
Explore 7E2I in 3D Show helices and sheets RCSB PDB PDBe
7E2I contains 40 α-helices and 17 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 182-185 | 4 | |
| α-helix | 190-209 | 20 | |
| α-helix | 212-214 | 3 | |
| α-helix | 231-242 | 12 | |
| β-strand | 300-307 | 8 | 3 |
| α-helix | 316-326 | 11 | |
| α-helix | 327-331 | 5 | |
| α-helix | 354-362 | 9 | |
| α-helix | 373-387 | 15 | |
| α-helix | 413-414 | 2 | |
| α-helix | 424-429 | 6 | |
| β-strand | 451-459 | 9 | 3 |
| α-helix | 464-471 | 8 | |
| β-strand | 482-488 | 7 | 3 |
| α-helix | 493-502 | 10 | |
| α-helix | 505-521 | 17 | |
| α-helix | 525-544 | 20 | |
| α-helix | 545-549 | 5 | |
| α-helix | 559-586 | 28 | |
| α-helix | 593-622 | 30 | |
| α-helix | 628-658 | 31 | |
| α-helix | 688-704 | 17 | |
| α-helix | 705-709 | 5 | |
| α-helix | 710-717 | 8 | |
| α-helix | 720-737 | 18 | |
| α-helix | 749-751 | 3 | |
| β-strand | 755 | 1 | 4 |
| α-helix | 760-764 | 5 | |
| α-helix | 769-771 | 3 | |
| α-helix | 773-778 | 6 | |
| α-helix | 785-787 | 3 | |
| β-strand | 788-789 | 2 | 5 |
| α-helix | 821-834 | 14 | |
| α-helix | 852-859 | 8 | |
| α-helix | 884-901 | 18 | |
| β-strand | 913-914 | 2 | 6 |
| β-strand | 920-922 | 3 | 6 |
| β-strand | 925-926 | 2 | 5 |
| α-helix | 937-952 | 16 | |
| α-helix | 972-1003 | 32 | |
| α-helix | 1006-1029 | 24 | |
| α-helix | 1037-1058 | 22 | |
| α-helix | 1069-1097 | 29 | |
| β-strand | 1102 | 1 | 4 |
| α-helix | 1103-1132 | 30 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 47 | 1 | 1 |
| α-helix | 72-75 | 4 | |
| β-strand | 76-78 | 3 | 2 |
| β-strand | 85-86 | 2 | 1 |
| β-strand | 97-99 | 3 | 2 |
| α-helix | 100-116 | 17 | |
| β-strand | 123-126 | 4 | 1 |
| α-helix | 139-142 | 4 | |
| β-strand | 145-149 | 5 | 1 |
| α-helix | 156-167 | 12 | |
| β-strand | 172-174 | 3 | 1 |
| β-strand | 181-184 | 4 | 1 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| Sonic hedgehog protein | G | protein | 462 | Homo sapiens | Q15465 (AlphaFold model) |
| Protein dispatched homolog 1 | D | protein | 1524 | Homo sapiens | Q96F81 (AlphaFold model) |
>7E2I_1 Sonic hedgehog protein (chains G) MLLLARCLLLVLVSSLLVCSGLACGPGRGFGKRRHPKKLTPLAYKQFIPNVAEKTLGASG RYEGKISRNSERFKELTPNYNPDIIFKDEENTGADRLMTQRCKDKLNALAISVMNQWPGV KLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRSKYGMLARLAVEAGFDWVYYESKAH IHCSVKAENSVAAKSGGCFPGSATVHLEQGGTKLVKDLSPGDRVLAADDQGRLLYSDFLT FLDRDDGAKKVFYVIETREPRERLLLTAAHLLFVAPHNDSATGEPEASSGSGPPSGGALG PRALFASRVRPGQRVYVVAERDGDRRLLPAAVHSVTLSEEAAGAYAPLTAQGTILINRVL ASCYAVIEEHSWAHRAFAPFRLAHALLAALAPARTDRGGDSGGGDRGGGGGRVALTAPGA ADAPGAGATAGIHWYSQLLYQIGTWLLDSEALHPLGMAVKSS
>7E2I_2 Protein dispatched homolog 1 (chains D) MAMSNGNNDFVVLSNSSIATSAANPSPLTPCDGDHAAQQLTPKEATRTKVSPNGCLQLNG TVKSSFLPLDNQRMPQMLPQCCHPCPYHHPLTSHSSHQECHPEAGPAAPSALASCCMQPH SEYSASLCPNHSPVYQTTCCLQPSPSFCLHHPWPDHFQHQPVQQHIANIRPSRPFKLPKS YAALIADWPVVVLGMCTMFIVVCALVGVLVPELPDFSDPLLGFEPRGTAIGQRLVTWNNM VKNTGYKATLANYPFKYADEQAKSHRDDRWSDDHYEREKREVDWNFHKDSFFCDVPSDRY SRVVFTSSGGETLWNLPAIKSMCNVDNSRIRSHPQFGDLCQRTTAASCCPSWTLGNYIAI LNNRSSCQKIVERDVSHTLKLLRTCAKHYQNGTLGPDCWDMAARRKDQLKCTNVPRKCTK YNAVYQILHYLVDKDFMTPKTADYATPALKYSMLFSPTEKGESMMNIYLDNFENWNSSDG VTTITGIEFGIKHSLFQDYLLMDTVYPAIAIVIVLLVMCVYTKSMFITLMTMFAIISSLI VSYFLYRVVFHFEFFPFMNLTALIILVGIGANNAFVLCDVWNYTKFDKPHAETSETVSIT LQHAALSMFVTSFTTAAAFYANYVSNITAIRCFGVYAGTAILVNYVLMVTWLPAVVVLHE RYLLNIFTCFKKPQQQIYDNKSCWTVACQKCHKVLFAISEASRIFFEKVLPCIVIKFRYL WLFWFLALTVGGAYIVCINPKMKLPSLELSEFQVFRSSHPFERYDAEYKKLFMFERVHHG EELHMPITVIWGVSPEDNGNPLNPKSKGKLTLDSSFNIASPASQAWILHFCQKLRNQTFF YQTDEQDFTSCFIETFKQWMENQDCDEPALYPCCSHWSFPYKQEIFELCIKRAIMELERS TGYHLDSKTPGPRFDINDTIRAVVLEFQSTYLFTLAYEKMHQFYKEVDSWISSELSSAPE GLSNGWFVSNLEFYDLQDSLSDGTLIAMGLSVAVAFSVMLLTTWNIIISLYAIISIAGTI FVTVGSLVLLGWELNVLESVTISVAVGLSVNFAVHYGVAYRLAPDPDREGKVIFSLSRVG SAMAMAALTTFVAGAMMMPSTVLAYTQLGTFMMLIMCISWAFATFFFQCMCRCLGPQGTC GQIPLPKKLQCSAFSHALSTSPSDKGQSKTHTINAYHLDPRGPKSELEHEFYELEPLASH SCTAPEKTTYEETHICSEFFNSQAKNLGMPVHAAYNSELSKSTESDAGSALLQPPLEQHT VCHFFSLNQRCSCPDAYKHLNYGPHSCQQMGDCLCHQCSPTTSSFVQIQNGVAPLKATHQ AVEGFVHPITHIHHCPCLQGRVKPAGMQNSLPRNFFLHPVQHIQAQEKIGKTNVHSLQRS IEEHLPKMAEPSSFVCRSTGSLLKTCCDPENKQRELCKNRDVSNLESSGGTENKAGGKVE LSLSQTDASVNSEHFNQNEPKVLFNHLMGEAGCRSCPNNSQSCGRIVRVKCNSVDCQMPN MEANVPAVLTHSELSGESLLIKTL
| ID | Name | Formula | Copies |
|---|---|---|---|
| NAG | 2-acetamido-2-deoxy-beta-D-glucopyranose | C8 H15 N O6 | 4 |
| Y01 | Cholesterol hemisuccinate | C31 H50 O4 | 7 |
| ZN | Zinc ion | Zn | 1 |
Structural insights into proteolytic activation of the human Dispatched1 transporter for Hedgehog morphogen release. Li, W., Wang, L., Wierbowski, B.M. et al. Nat Commun (2021) 12:6966-6966. DOI 10.1038/s41467-021-27257-w · PubMed
Other PDB entries of the same protein (UniProt Q15465 (AlphaFold model), which also has an AlphaFold model), best resolution first:
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