7EGF: TFIID lobe A subcomplex
TFIID lobe A subcomplex. Determined by electron microscopy at 3.16 Å resolution. Released 5 May 2021.
- Method
- Electron microscopy
- Resolution
- 3.16 Å
- Organism
- Homo sapiens
- Chains
- 10
- Atoms
- 13,303
- Mol. weight
- 517.74 kDa
- Released
- 5 May 2021
Explore 7EGF in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
7EGF contains 67 α-helices and 63 β-strands across 10 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain c: 5 helices, 2 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 2-15 | 14 | |
| α-helix | 17-20 | 4 | |
| β-strand | 25-27 | 3 | 3 |
| α-helix | 28-55 | 28 | |
| α-helix | 63-73 | 11 | |
| α-helix | 77-86 | 10 | |
| β-strand | 101 | 1 | 4 |
Chain d: 9 helices, 4 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 844-848 | 5 | |
| α-helix | 850-854 | 5 | |
| α-helix | 855-857 | 3 | |
| β-strand | 865 | 1 | 5 |
| α-helix | 875-884 | 10 | |
| β-strand | 893-894 | 2 | 6 |
| α-helix | 899-921 | 23 | |
| α-helix | 923-925 | 3 | |
| β-strand | 934-936 | 3 | 7 |
| α-helix | 940-967 | 28 | |
| β-strand | 1056 | 1 | 8 |
| α-helix | 1060-1068 | 9 | |
| α-helix | 1077-1082 | 6 | |
Chain e: 17 helices, 33 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 214-227 | 14 | |
| α-helix | 230-254 | 25 | |
| α-helix | 257-267 | 11 | |
| α-helix | 277-284 | 8 | |
| α-helix | 288-291 | 4 | |
| β-strand | 307 | 1 | 9 |
| β-strand | 310 | 1 | 10 |
| α-helix | 311-321 | 11 | |
| α-helix | 330-333 | 4 | |
| β-strand | 339 | 1 | 9 |
| β-strand | 342 | 1 | 10 |
| α-helix | 376-377 | 2 | |
| α-helix | 421-423 | 3 | |
| α-helix | 429-432 | 4 | |
| α-helix | 434-448 | 15 | |
| α-helix | 458-460 | 3 | |
| β-strand | 461-466 | 6 | 11 |
| β-strand | 473-478 | 6 | 12 |
| β-strand | 484-489 | 6 | 12 |
| β-strand | 493-498 | 6 | 12 |
| α-helix | 503-504 | 2 | |
| β-strand | 506-507 | 2 | 13 |
| α-helix | 508 | 1 | |
| α-helix | 510-515 | 6 | |
| α-helix | 517 | 1 | |
| α-helix | 523-526 | 4 | |
| β-strand | 528-529 | 2 | 13 |
| β-strand | 536-540 | 5 | 12 |
| β-strand | 546-550 | 5 | 14 |
| β-strand | 558-562 | 5 | 14 |
| β-strand | 567-570 | 4 | 14 |
| β-strand | 577-581 | 5 | 14 |
| β-strand | 588-593 | 6 | 15 |
| β-strand | 600-604 | 5 | 15 |
| β-strand | 609-612 | 4 | 15 |
| β-strand | 621-622 | 2 | 15 |
| β-strand | 632-635 | 4 | 16 |
| β-strand | 641-645 | 5 | 16 |
| β-strand | 651-655 | 5 | 16 |
| β-strand | 661-665 | 5 | 16 |
| β-strand | 672-677 | 6 | 17 |
| β-strand | 683-688 | 6 | 17 |
| β-strand | 692-697 | 6 | 17 |
| β-strand | 703-707 | 5 | 17 |
| β-strand | 716-719 | 4 | 18 |
| β-strand | 725-729 | 5 | 18 |
| β-strand | 734-739 | 6 | 18 |
| β-strand | 771-774 | 4 | 18 |
| β-strand | 782-785 | 4 | 11 |
| β-strand | 791-794 | 4 | 11 |
Chain f: 7 helices, 5 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 17-26 | 10 | |
| α-helix | 33-60 | 28 | |
| α-helix | 68-75 | 8 | |
| α-helix | 80-82 | 3 | |
| β-strand | 95-97 | 3 | 19 |
| β-strand | 104-106 | 3 | 19 |
| β-strand | 111-113 | 3 | 3 |
| α-helix | 115-118 | 4 | |
| α-helix | 120-126 | 7 | |
| β-strand | 127 | 1 | 4 |
| β-strand | 131-137 | 7 | 11 |
| α-helix | 142-143 | 2 | |
Chain i: 6 helices, 2 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 19-25 | 7 | |
| α-helix | 34-60 | 27 | |
| α-helix | 68-76 | 9 | |
| α-helix | 84-88 | 5 | |
| α-helix | 89-99 | 11 | |
| α-helix | 103-106 | 4 | |
| β-strand | 121 | 1 | 14 |
| β-strand | 127-129 | 3 | 7 |
Chain j: 4 helices, 2 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 115-123 | 9 | |
| α-helix | 132-138 | 7 | |
| α-helix | 149-176 | 28 | |
| β-strand | 194-195 | 2 | 3 |
| α-helix | 197-204 | 8 | |
| β-strand | 217 | 1 | 19 |
Chain k: 4 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 117-126 | 10 | |
| α-helix | 131-142 | 12 | |
| α-helix | 148-175 | 28 | |
| α-helix | 185-196 | 12 | |
Chain l: 6 helices, 2 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 62-68 | 7 | |
| β-strand | 74 | 1 | 8 |
| α-helix | 78-105 | 28 | |
| β-strand | 110-111 | 2 | 6 |
| α-helix | 114-119 | 6 | |
| α-helix | 120-124 | 5 | |
| α-helix | 134-138 | 5 | |
| α-helix | 146-160 | 15 | |
2 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| TATA-box-binding protein | P | protein | 339 | Homo sapiens | P20226 (AlphaFold model) |
| Transcription initiation factor TFIID subunit 3 | c | protein | 929 | Homo sapiens | Q5VWG9 (AlphaFold model) |
| Transcription initiation factor TFIID subunit 4 | d | protein | 1085 | Homo sapiens | O00268 (AlphaFold model) |
| Transcription initiation factor TFIID subunit 5 | e | protein | 800 | Homo sapiens | Q15542 (AlphaFold model) |
| Transcription initiation factor TFIID subunit 6 | f | protein | 677 | Homo sapiens | P49848 |
| Transcription initiation factor TFIID subunit 9 | i | protein | 264 | Homo sapiens | Q16594 |
| Transcription initiation factor TFIID subunit 10 | j | protein | 218 | Homo sapiens | Q12962 |
| Transcription initiation factor TFIID subunit 11 | k | protein | 211 | Homo sapiens | Q15544 |
| Transcription initiation factor TFIID subunit 12 | l | protein | 161 | Homo sapiens | Q16514 |
| Transcription initiation factor TFIID subunit 13 | m | protein | 124 | Homo sapiens | Q15543 |
Sequence of entity 1 (P), FASTA
>7EGF_1 TATA-box-binding protein (chains P)
MDQNNSLPPYAQGLASPQGAMTPGIPIFSPMMPYGTGLTPQPIQNTNSLSILEEQQRQQQ
QQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQAVAAAAVQQSTSQQATQGTSGQAPQ
LFHSQTLTTAPLPGTTPLYPSPMTPMTPITPATPASESSGIVPQLQNIVSTVNLGCKLDL
KTIALRARNAEYNPKRFAAVIMRIREPRTTALIFSSGKMVCTGAKSEEQSRLAARKYARV
VQKLGFPAKFLDFKIQNMVGSCDVKFPIRLEGLVLTHQQFSSYEPELFPGLIYRMIKPRI
VLLIFVSGKVVLTGAKVRAEIYEAFENIYPILKGFRKTT
Sequence of entity 2 (c), FASTA
>7EGF_2 Transcription initiation factor TFIID subunit 3 (chains c)
MCESYSRSLLRVSVAQICQALGWDSVQLSACHLLTDVLQRYLQQLGRGCHRYSELYGRTD
PILDDVGEAFQLMGVSLHELEDYIHNIEPVTFPHQIPSFPVSKNNVLQFPQPGSKDAEER
KEYIPDYLPPIVSSQEEEEEEQVPTDGGTSAEAMQVPLEEDDELEEEEIINDENFLGKRP
LDSPEAEELPAMKRPRLLSTKGDTLDVVLLEAREPLSSINTQKIPPMLSPVHVQDSTDLA
PPSPEPPMLAPVAKSQMPTAKPLETKSFTPKTKTKTSSPGQKTKSPKTAQSPAMVGSPIR
SPKTVSKEKKSPGRSKSPKSPKSPKVTTHIPQTPVRPETPNRTPSATLSEKISKETIQVK
QIQTPPDAGKLNSENQPKKAVVADKTIEASIDAVIARACAEREPDPFEFSSGSESEGDIF
TSPKRISGPECTTPKASTSANNFTKSGSTPLPLSGGTSSSDNSWTMDASIDEVVRKAKLG
TPSNMPPNFPYISSPSVSPPTPEPLHKVYEEKTKLPSSVEVKKKLKKELKTKMKKKEKQR
DREREKDKNKDKSKEKDKVKEKEKDKETGRETKYPWKEFLKEEEADPYKFKIKEFEDVDP
KVKLKDGLVRKEKEKHKDKKKDREKGKKDKDKREKEKVKDKGREDKMKAPAPPLVLPPKE
LALPLFSPATASRVPAMLPSLLPVLPEKLFEEKEKVKEKEKKKDKKEKKKKKEKEKEKKE
KEREKEKREREKREKEKEKHKHEKIKVEPVALAPSPVIPRLTLRVGAGQDKIVISKVVPA
PEAKPAPSQNRPKTPPPAPAPAPGPMLVSPAPVPLPLLAQAAAGPALLPSPGPAASGASA
KAPVRSVVTETVSTYVIRDEWGNQIWICPGCNKPDDGSPMIGCDDCDDWYHWPCVGIMTA
PPEEMQWFCPKCANKKKDKKHKKRKHRAH
Sequence of entity 3 (d), FASTA
>7EGF_3 Transcription initiation factor TFIID subunit 4 (chains d)
MAAGSDLLDEVFFNSEVDEKVVSDLVGSLESQLAASAAHHHHLAPRTPEVRAAAAGALGN
HVVSGSPAGAAGAGPAAPAEGAPGAAPEPPPAGRARPGGGGPQRPGPPSPRRPLVPAGPA
PPAAKLRPPPEGSAGSCAPVPAAAAVAAGPEPAPAGPAKPAGPAALAARAGPGPGPGPGP
GPGPGPGKPAGPGAAQTLNGSAALLNSHHAAAPAVSLVNNGPAALLPLPKPAAPGTVIQT
PPFVGAAAPPAPAAPSPPAAPAPAAPAAAPPPPPPAPATLARPPGHPAGPPTAAPAVPPP
AAAQNGGSAGAAPAPAPAAGGPAGVSGQPGPGAAAAAPAPGVKAESPKRVVQAAPPAAQT
LAASGPASTAASMVIGPTMQGALPSPAAVPPPAPGTPTGLPKGAAGAVTQSLSRTPTATT
SGIRATLTPTVLAPRLPQPPQNPTNIQNFQLPPGMVLVRSENGQLLMIPQQALAQMQAQA
HAQPQTTMAPRPATPTSAPPVQISTVQAPGTPIIARQVTPTTIIKQVSQAQTTVQPSATL
QRSPGVQPQLVLGGAAQTASLGTATAVQTGTPQRTVPGATTTSSAATETMENVKKCKNFL
STLIKLASSGKQSTETAANVKELVQNLLDGKIEAEDFTSRLYRELNSSPQPYLVPFLKRS
LPALRQLTPDSAAFIQQSQQQPPPPTSQATTALTAVVLSSSVQRTAGKTAATVTSALQPP
VLSLTQPTQVGVGKQGQPTPLVIQQPPKPGALIRPPQVTLTQTPMVALRQPHNRIMLTTP
QQIQLNPLQPVPVVKPAVLPGTKALSAVSAQAAAAQKNKLKEPGGGSFRDDDDINDVASM
AGVNLSEESARILATNSELVGTLTRSCKDETFLLQAPLQRRILEIGKKHGITELHPDVVS
YVSHATQQRLQNLVEKISETAQQKNFSYKDDDRYEQASDVRAQLKFFEQLDQIEKQRKDE
QEREILMRAAKSRSRQEDPEQLRLKQKAKEMQQQELAQMRQRDANLTALAAIGPRKKRKV
DCPGPGSGAEGSGPGSVVPGSSGVGTPRQFTRQRITRVNLRDLIFCLENERETSHSLLLY
KAFLK
Sequence of entity 4 (e), FASTA
>7EGF_4 Transcription initiation factor TFIID subunit 5 (chains e)
MAALAEEQTEVAVKLEPEGPPTLLPPQAGDGAGEGSGGTTNNGPNGGGGNVAASSSTGGD
GGTPKPTVAVSAAAPAGAAPVPAAAPDAGAPHDRQTLLAVLQFLRQSKLREAEEALRREA
GLLEEAVAGSGAPGEVDSAGAEVTSALLSRVTASAPGPAAPDPPGTGASGATVVSGSASG
PAAPGKVGSVAVEDQPDVSAVLSAYNQQGDPTMYEEYYSGLKHFIECSLDCHRAELSQLF
YPLFVHMYLELVYNQHENEAKSFFEKFHGDQECYYQDDLRVLSSLTKKEHMKGNETMLDF
RTSKFVLRISRDSYQLLKRHLQEKQNNQIWNIVQEHLYIDIFDGMPRSKQQIDAMVGSLA
GEAKREANKSKVFFGLLKEPEIEVPLDDEDEEGENEEGKPKKKKPKKDSIGSKSKKQDPN
APPQNRIPLPELKDSDKLDKIMNMKETTKRVRLGPDCLPSICFYTFLNAYQGLTAVDVTD
DSSLIAGGFADSTVRVWSVTPKKLRSVKQASDLSLIDKESDDVLERIMDEKTASELKILY
GHSGPVYGASFSPDRNYLLSSSEDGTVRLWSLQTFTCLVGYKGHNYPVWDTQFSPYGYYF
VSGGHDRVARLWATDHYQPLRIFAGHLADVNCTRFHPNSNYVATGSADRTVRLWDVLNGN
CVRIFTGHKGPIHSLTFSPNGRFLATGATDGRVLLWDIGHGLMVGELKGHTDTVCSLRFS
RDGEILASGSMDNTVRLWDAIKAFEDLETDDFTTATGHINLPENSQELLLGTYMTKSTPV
VHLHFTRRNLVLAAGAYSPQ
Sequence of entity 5 (f), FASTA
>7EGF_5 Transcription initiation factor TFIID subunit 6 (chains f)
MAEEKKLKLSNTVLPSESMKVVAESMGIAQIQEETCQLLTDEVSYRIKEIAQDALKFMHM
GKRQKLTTSDIDYALKLKNVEPLYGFHAQEFIPFRFASGGGRELYFYEEKEVDLSDIINT
PLPRVPLDVCLKAHWLSIEGCQPAIPENPPPAPKEQQKAEATEPLKSAKPGQEEDGPLKG
KGQGATTADGKGKEKKAPPLLEGAPLRLKPRSIHELSVEQQLYYKEITEACVGSCEAKRA
EALQSIATDPGLYQMLPRFSTFISEGVRVNVVQNNLALLIYLMRMVKALMDNPTLYLEKY
VHELIPAVMTCIVSRQLCLRPDVDNHWALRDFAARLVAQICKHFSTTTNNIQSRITKTFT
KSWVDEKTPWTTRYGSIAGLAELGHDVIKTLILPRLQQEGERIRSVLDGPVLSNIDRIGA
DHVQSLLLKHCAPVLAKLRPPPDNQDAYRAEFGSLGPLLCSQVVKARAQAALQAQQVNRT
TLTITQPRPTLTLSQAPQPGPRTPGLLKVPGSIALPVQTLVSARAAAPPQPSPPPTKFIV
MSSSSSAPSTQQVLSLSTSAPGSGSTTTSPVTTTVPSVQPIVKLVSTATTAPPSTAPSGP
GSVQKYIVVSLPPTGEGKGGPTSHPSPVPPPASSPSPLSGSALCGGKQEAGDSPPPAPGT
PKANGSQPNSGSPQPAP
Sequence of entity 6 (i), FASTA
>7EGF_6 Transcription initiation factor TFIID subunit 9 (chains i)
MESGKTASPKSMPKDAQMMAQILKDMGITEYEPRVINQMLEFAFRYVTTILDDAKIYSSH
AKKATVDADDVRLAIQCRADQSFTSPPPRDFLLDIARQRNQTPLPLIKPYSGPRLPPDRY
CLTAPNYRLKSLQKKASTSAGRITVPRLSVGSVTSRPSTPTLGTPTPQTMSVSTKVGTPM
SLTGQRFTVQMPTSQSPAVKASIPATSAVQNVLINPSLIGSKNILITTNMMSSQNTANES
SNALKRKREDDDDDDDDDDDYDNL
Sequence of entity 7 (j), FASTA
>7EGF_7 Transcription initiation factor TFIID subunit 10 (chains j)
MSCSGSGADPEAAPASAASAPGPAPPVSAPAALPSSTAAENKASPAGTAGGPGAGAAAGG
TGPLAARAGEPAERRGAAPVSAGGAAPPEGAISNGVYVLPSAANGDVKPVVSSTPLVDFL
MQLEDYTPTIPDAVTGYYLNRAGFEASDPRIIRLISLAAQKFISDIANDALQHCKMKGTA
SGSSRSKSKDRKYTLTMEDLTPALSEYGINVKKPHYFT
Sequence of entity 8 (k), FASTA
>7EGF_8 Transcription initiation factor TFIID subunit 11 (chains k)
MDDAHESPSDKGGETGESDETAAVPGDPGATDTDGIPEETDGDADVDLKEAAAEEGELES
QDVSDLTTVEREDSSLLNPAAKKLKIDTKEKKEKKQKVDEDEIQKMQILVSSFSEEQLNR
YEMYRRSAFPKAAIKRLIQSITGTSVSQNVVIAMSGISKVFVGEVVEEALDVCEKWGEMP
PLQPKHMREAVRRLKSKGQIPNSKHKKIIFF
Sequence of entity 9 (l), FASTA
>7EGF_9 Transcription initiation factor TFIID subunit 12 (chains l)
MNQFGPSALINLSNFSSIKPEPASTPPQGSMANSTAVVKIPGTPGAGGRLSPENNQVLTK
KKLQDLVREVDPNEQLDEDVEEMLLQIADDFIESVVTAACQLARHRKSSTLEVKDVQLHL
ERQWNMWIPGFGSEEIRPYKKACTTEAHKQRMALIRKTTKK
Sequence of entity 10 (m), FASTA
>7EGF_10 Transcription initiation factor TFIID subunit 13 (chains m)
MADEEEDPTFEEENEEIGGGAEGGQGKRKRLFSKELRCMMYGFGDDQNPYTESVDILEDL
VIEFITEMTHKAMSIGRQGRVQVEDIVFLIRKDPRKFARVKDLLTMNEELKRARKAFDEA
NYGS
Primary citation
Structural insights into preinitiation complex assembly on core promoters. Chen, X., Qi, Y., Wu, Z. et al. Science (2021). DOI 10.1126/science.aba8490 · PubMed
Other PDB entries of the same protein (UniProt P20226 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 1CDW 1.9 Å, Human tbp core domain complexed with DNA
- 4ROC 1.9 Å, Human TFIIB-related factor 2 (Brf2) and TBP bound to U6#2 promoter
- 1NVP 2.1 Å, Human tfiia/tbp/dna complex
- 4ROE 2.2 Å, Human TFIIB-related factor 2 (Brf2) and TBP bound to RPPH1 promoter
- 7NVU 2.5 Å, RNA polymerase II core pre-initiation complex with open promoter DNA
- 1JFI 2.62 Å, Crystal Structure of the NC2-TBP-DNA Ternary Complex
- 1C9B 2.65 Å, Crystal structure of a human tbp core domain-human tfiib core domain complex bound to an…
- 4ROD 2.7 Å, Human TFIIB-related factor 2 (Brf2) and TBP bound to TRNAU1 promoter
- 5N9G 2.7 Å, TFIIIB -TBP/Brf2/DNA and SANT domain of Bdp1-
- 7NVS 2.8 Å, RNA polymerase II core pre-initiation complex with closed promoter DNA in proximal…
- 1TGH 2.9 Å, Tata binding protein (tbp)/dna complex
- 7NVT 2.9 Å, RNA polymerase II core pre-initiation complex with closed promoter DNA in distal position
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