7EGH: TFIID lobe C subcomplex
TFIID lobe C subcomplex. Determined by electron microscopy at 3.04 Å resolution. Released 12 May 2021.
- Method
- Electron microscopy
- Resolution
- 3.04 Å
- Organism
- Homo sapiens
- Chains
- 8
- Atoms
- 20,543
- Mol. weight
- 621.38 kDa
- Released
- 12 May 2021
Explore 7EGH in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
7EGH contains 117 α-helices and 65 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain A: 34 helices, 17 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 347-353 | 7 | |
| α-helix | 399-402 | 4 | |
| α-helix | 410-413 | 4 | |
| α-helix | 469-471 | 3 | |
| α-helix | 473-477 | 5 | |
| α-helix | 480-483 | 4 | |
| α-helix | 497-500 | 4 | |
| α-helix | 570-573 | 4 | |
| α-helix | 593-596 | 4 | |
| α-helix | 609-613 | 5 | |
| α-helix | 621-623 | 3 | |
| β-strand | 634-638 | 5 | 1 |
| α-helix | 641-656 | 16 | |
| α-helix | 668-671 | 4 | |
| β-strand | 679-683 | 5 | 1 |
| α-helix | 692-693 | 2 | |
| β-strand | 697-704 | 8 | 1 |
| α-helix | 705-707 | 3 | |
| α-helix | 714-716 | 3 | |
| β-strand | 721-723 | 3 | 1 |
| α-helix | 734-735 | 2 | |
| β-strand | 739-744 | 6 | 1 |
| β-strand | 748-756 | 9 | 1 |
| α-helix | 757-758 | 2 | |
| β-strand | 761-766 | 6 | 1 |
| β-strand | 771-775 | 5 | 1 |
| β-strand | 778-782 | 5 | 1 |
| β-strand | 786-787 | 2 | 1 |
| α-helix | 790-793 | 4 | |
| α-helix | 797-816 | 20 | |
| β-strand | 824-825 | 2 | 2 |
| α-helix | 827-833 | 7 | |
| α-helix | 839-847 | 9 | |
| β-strand | 850-853 | 4 | 2 |
| β-strand | 861-864 | 4 | 2 |
| α-helix | 865 | 1 | |
| α-helix | 873-879 | 7 | |
| α-helix | 882-900 | 19 | |
| α-helix | 925-928 | 4 | |
| α-helix | 931-943 | 13 | |
| β-strand | 947-948 | 2 | 3 |
| β-strand | 967-968 | 2 | 3 |
| α-helix | 993-997 | 5 | |
| α-helix | 999-1010 | 12 | |
| α-helix | 1014-1018 | 5 | |
| α-helix | 1022-1034 | 13 | |
| α-helix | 1056-1078 | 23 | |
| β-strand | 1165-1170 | 6 | 4 |
| β-strand | 1180-1185 | 6 | 4 |
| α-helix | 1188-1203 | 16 | |
| α-helix | 1217-1241 | 25 | |
Chain B: 44 helices, 39 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 26-39 | 14 | 5 |
| β-strand | 44-55 | 12 | 5 |
| β-strand | 63-65 | 3 | 6 |
| β-strand | 66 | 1 | 7 |
| β-strand | 71-77 | 7 | 5 |
| β-strand | 80-81 | 2 | 5 |
| β-strand | 83-86 | 4 | 6 |
| α-helix | 103-116 | 14 | |
| β-strand | 126-129 | 4 | 6 |
| α-helix | 130-131 | 2 | |
| α-helix | 132-134 | 3 | |
| α-helix | 135-138 | 4 | |
| β-strand | 144-152 | 9 | 5 |
| β-strand | 159-162 | 4 | 8 |
| α-helix | 170-173 | 4 | |
| β-strand | 176-179 | 4 | 8 |
| α-helix | 186-188 | 3 | |
| β-strand | 192 | 1 | 7 |
| β-strand | 200-209 | 10 | 5 |
| β-strand | 213-216 | 4 | 8 |
| β-strand | 219-225 | 7 | 5 |
| α-helix | 226 | 1 | |
| β-strand | 231-242 | 12 | 5 |
| α-helix | 244-246 | 3 | |
| β-strand | 249-252 | 4 | 8 |
| α-helix | 254 | 1 | |
| β-strand | 255-258 | 4 | 9 |
| β-strand | 265-269 | 5 | 9 |
| α-helix | 274-280 | 7 | |
| α-helix | 284-294 | 11 | |
| α-helix | 298-299 | 2 | |
| β-strand | 304-309 | 6 | 9 |
| β-strand | 316-319 | 4 | 9 |
| β-strand | 322-326 | 5 | 9 |
| α-helix | 327-329 | 3 | |
| α-helix | 339-352 | 14 | |
| α-helix | 353-358 | 6 | |
| β-strand | 360-361 | 2 | 10 |
| α-helix | 367-408 | 42 | |
| α-helix | 438-440 | 3 | |
| α-helix | 443-463 | 21 | |
| α-helix | 466-483 | 18 | |
| α-helix | 493-495 | 3 | |
| β-strand | 497-498 | 2 | 10 |
| α-helix | 500-511 | 12 | |
| α-helix | 516-522 | 7 | |
| β-strand | 528 | 1 | 11 |
| β-strand | 529-538 | 10 | 12 |
| β-strand | 543-550 | 8 | 12 |
| β-strand | 557 | 1 | 11 |
| β-strand | 563-570 | 8 | 13 |
| β-strand | 573-580 | 8 | 13 |
| β-strand | 587-591 | 5 | 12 |
| α-helix | 596-599 | 4 | |
| α-helix | 603 | 1 | |
| β-strand | 604-605 | 2 | 14 |
| β-strand | 611-612 | 2 | 14 |
| β-strand | 625-630 | 6 | 13 |
| β-strand | 638-643 | 6 | 12 |
| α-helix | 646-655 | 10 | |
| α-helix | 659-668 | 10 | |
| α-helix | 669-671 | 3 | |
| α-helix | 675-685 | 11 | |
| β-strand | 688 | 1 | 15 |
| β-strand | 690 | 1 | 15 |
| α-helix | 692-708 | 17 | |
| α-helix | 717 | 1 | |
| α-helix | 720-727 | 8 | |
| β-strand | 728 | 1 | 16 |
| β-strand | 736 | 1 | 16 |
| α-helix | 737-739 | 3 | |
| α-helix | 746-757 | 12 | |
| α-helix | 769-780 | 12 | |
| α-helix | 792-804 | 13 | |
| α-helix | 807-809 | 3 | |
| α-helix | 824-842 | 19 | |
| α-helix | 849-863 | 15 | |
| α-helix | 872-877 | 6 | |
| α-helix | 884-900 | 17 | |
| α-helix | 904-915 | 12 | |
| α-helix | 920-932 | 13 | |
| α-helix | 947-958 | 12 | |
| α-helix | 967-979 | 13 | |
Chain f: 16 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 215-217 | 3 | |
| α-helix | 218-230 | 13 | |
| α-helix | 236-247 | 12 | |
| α-helix | 256-268 | 13 | |
| α-helix | 276-290 | 15 | |
| α-helix | 301-313 | 13 | |
| α-helix | 326-340 | 15 | |
| α-helix | 351-363 | 13 | |
| α-helix | 370-383 | 14 | |
| α-helix | 385-387 | 3 | |
| α-helix | 388-392 | 5 | |
| α-helix | 393-397 | 5 | |
| α-helix | 398-403 | 6 | |
| α-helix | 416-437 | 22 | |
| α-helix | 446-449 | 4 | |
| α-helix | 455-472 | 18 | |
Chain F: 16 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 209-212 | 4 | |
| α-helix | 218-230 | 13 | |
| α-helix | 236-248 | 13 | |
| α-helix | 250-252 | 3 | |
| α-helix | 256-269 | 14 | |
| α-helix | 276-290 | 15 | |
| α-helix | 301-313 | 13 | |
| α-helix | 324-343 | 20 | |
| α-helix | 352-363 | 12 | |
| α-helix | 370-381 | 12 | |
| α-helix | 385-390 | 6 | |
| α-helix | 393-395 | 3 | |
| α-helix | 396-408 | 13 | |
| α-helix | 416-437 | 22 | |
| α-helix | 447-451 | 5 | |
| α-helix | 456-472 | 17 | |
Chain G: 4 helices, 7 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 14-19 | 6 | 1 |
| α-helix | 22-34 | 13 | |
| α-helix | 38-42 | 5 | |
| β-strand | 43-47 | 5 | 1 |
| β-strand | 54-58 | 5 | 1 |
| β-strand | 61-79 | 19 | 1 |
| β-strand | 84-97 | 14 | 1 |
| β-strand | 131 | 1 | 1 |
| α-helix | 143-147 | 5 | |
| α-helix | 162-175 | 14 | |
| β-strand | 179-183 | 5 | 4 |
Chain H: 3 helices, 2 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 172-196 | 25 | |
| β-strand | 200-201 | 2 | 17 |
| β-strand | 213-214 | 2 | 17 |
| α-helix | 215-216 | 2 | |
| α-helix | 222-227 | 6 | |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Transcription initiation factor TFIID subunit 1 | A | protein | 1872 | Homo sapiens | P21675 (AlphaFold model) |
| Transcription initiation factor TFIID subunit 2 | B | protein | 1199 | Homo sapiens | Q6P1X5 (AlphaFold model) |
| Transcription initiation factor TFIID subunit 6 | F, f | protein | 677 | Homo sapiens | P49848 (AlphaFold model) |
| Transcription initiation factor TFIID subunit 7 | G | protein | 349 | Homo sapiens | Q15545 (AlphaFold model) |
| Transcription initiation factor TFIID subunit 8 | H | protein | 310 | Homo sapiens | Q7Z7C8 |
| DNA (45-mer) | X | DNA | 72 | Homo sapiens | |
| DNA (45-mer) | Y | DNA | 94 | Homo sapiens | |
Sequence of entity 1 (A), FASTA
>7EGH_1 Transcription initiation factor TFIID subunit 1 (chains A)
MGPGCDLLLRTAATITAAAIMSDTDSDEDSAGGGPFSLAGFLFGNINGAGQLEGESVLDD
ECKKHLAGLGALGLGSLITELTANEELTGTDGALVNDEGWVRSTEDAVDYSDINEVAEDE
SRRYQQTMGSLQPLCHSDYDEDDYDADCEDIDCKLMPPPPPPPGPMKKDKDQDSITGEKV
DFSSSSDSESEMGPQEATQAESEDGKLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLR
FLRLFGPGKNVPSVWRSARRKRKKKHRELIQEEQIQEVECSVESEVSQKSLWNYDYAPPP
PPEQCLSDDEITMMAPVESKFSQSTGDIDKVTDTKPRVAEWRYGPARLWYDMLGVPEDGS
GFDYGFKLRKTEHEPVIKSRMIEEFRKLEENNGTDLLADENFLMVTQLHWEDDIIWDGED
VKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDKPWYSIFPIDNEDLVYGRW
EDNIIWDAQAMPRLLEPPVLTLDPNDENLILEIPDEKEEATSNSPSKESKKESSLKKSRI
LLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQ
PFFPTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGG
EMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYG
ETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYYIRELVDIF
VVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSES
SIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYYSMIAAEQRLKD
AGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCG
EGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKL
SRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSST
EVLSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMLLAAGSAA
SGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKPAVIDAYVRIRTT
KDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDL
KLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEG
TKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTD
PMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYP
SREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLD
DDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIVNPMDLETIRKNISKH
KYQSRESFLDDVNLILANSVKYNGPESQYTKTAQEIVNVCYQTLTEYDEHLTQLEKDICT
AKEAALEEAELESLDPMTPGPYTPQPPDLYDTNTSLSMSRDASVFQDESNMSVLDIPSAT
PEKQVTQEGEDGDGDLADEEEGTVQQPQASVLYEDLLMSEGEDDEEDAGSDEEGDNPFSA
IQLSESGSDSDVGSGGIRPKQPRMLQENTRMDMENEESMMSYEGDGGEASHGLEDSNISY
GSYEEPDPKSNTQDTSFSSIGGYEVSEEEEDEEEEEQRSGPSVLSQVHLSEDEEDSEDFH
SIAGDSDLDSDE
Sequence of entity 2 (B), FASTA
>7EGH_2 Transcription initiation factor TFIID subunit 2 (chains B)
MPLTGVEPARMNRKKGDKGFESPRPYKLTHQVVCINNINFQRKSVVGFVELTIFPTVANL
NRIKLNSKQCRIYRVRINDLEAAFIYNDPTLEVCHSESKQRNLNYFSNAYAAAVSAVDPD
AGNGELCIKVPSELWKHVDELKVLKIHINFSLDQPKGGLHFVVPSVEGSMAERGAHVFSC
GYQNSTRFWFPCVDSYSELCTWKLEFTVDAAMVAVSNGDLVETVYTHDMRKKTFHYMLTI
PTAASNISLAIGPFEILVDPYMHEVTHFCLPQLLPLLKHTTSYLHEVFEFYEEILTCRYP
YSCFKTVFIDEAYVEVAAYASMSIFSTNLLHSAMIIDETPLTRRCLAQSLAQQFFGCFIS
RMSWSDEWVLKGISGYIYGLWMKKTFGVNEYRHWIKEELDKIVAYELKTGGVLLHPIFGG
GKEKDNPASHLHFSIKHPHTLSWEYYSMFQCKAHLVMRLIENRISMEFMLQVFNKLLSLA
STASSQKFQSHMWSQMLVSTSGFLKSISNVSGKDIQPLIKQWVDQSGVVKFYGSFAFNRK
RNVLELEIKQDYTSPGTQKYVGPLKVTVQELDGSFNHTLQIEENSLKHDIPCHSKSRRNK
KKKIPLMNGEEVDMDLSAMDADSPLLWIRIDPDMSVLRKVEFEQADFMWQYQLRYERDVV
AQQESILALEKFPTPASRLALTDILEQEQCFYRVRMSACFCLAKIANSMVSTWTGPPAMK
SLFTRMFCCKSCPNIVKTNNFMSFQSYFLQKTMPVAMALLRDVHNLCPKEVLTFILDLIK
YNDNRKNKFSDNYYRAEMIDALANSVTPAVSVNNEVRTLDNLNPDVRLILEEITRFLNME
KLLPSYRHTITVSCLRAIRVLQKNGHVPSDPALFKSYAEYGHFVDIRIAALEAVVDYTKV
DRSYEELQWLLNMIQNDPVPYVRHKILNMLTKNPPFTKNMESPLCNEALVDQLWKLMNSG
TSHDWRLRCGAVDLYFTLFGLSRPSCLPLPELGLVLNLKEKKAVLNPTIIPESVAGNQEA
ANNPSSHPQLVGFQNPFSSSQDEEEIDMDTVHDSQAFISHHLNMLERPSTPGLSKYRPAS
SRSALIPQHSAGCDSTPTTKPQWSLELARKGTGKEQAPLEMSMHPAASAPLSVFTKESTA
SKHSDHHHHHHHEHKKKKKKHKHKHKHKHKHDSKEKDKEPFTFSSPASGRSIRSPSLSD
Sequence of entity 3 (F, f), FASTA
>7EGH_3 Transcription initiation factor TFIID subunit 6 (chains F, f)
MAEEKKLKLSNTVLPSESMKVVAESMGIAQIQEETCQLLTDEVSYRIKEIAQDALKFMHM
GKRQKLTTSDIDYALKLKNVEPLYGFHAQEFIPFRFASGGGRELYFYEEKEVDLSDIINT
PLPRVPLDVCLKAHWLSIEGCQPAIPENPPPAPKEQQKAEATEPLKSAKPGQEEDGPLKG
KGQGATTADGKGKEKKAPPLLEGAPLRLKPRSIHELSVEQQLYYKEITEACVGSCEAKRA
EALQSIATDPGLYQMLPRFSTFISEGVRVNVVQNNLALLIYLMRMVKALMDNPTLYLEKY
VHELIPAVMTCIVSRQLCLRPDVDNHWALRDFAARLVAQICKHFSTTTNNIQSRITKTFT
KSWVDEKTPWTTRYGSIAGLAELGHDVIKTLILPRLQQEGERIRSVLDGPVLSNIDRIGA
DHVQSLLLKHCAPVLAKLRPPPDNQDAYRAEFGSLGPLLCSQVVKARAQAALQAQQVNRT
TLTITQPRPTLTLSQAPQPGPRTPGLLKVPGSIALPVQTLVSARAAAPPQPSPPPTKFIV
MSSSSSAPSTQQVLSLSTSAPGSGSTTTSPVTTTVPSVQPIVKLVSTATTAPPSTAPSGP
GSVQKYIVVSLPPTGEGKGGPTSHPSPVPPPASSPSPLSGSALCGGKQEAGDSPPPAPGT
PKANGSQPNSGSPQPAP
Sequence of entity 4 (G), FASTA
>7EGH_4 Transcription initiation factor TFIID subunit 7 (chains G)
MSKSKDDAPHELESQFILRLPPEYASTVRRAVQSGHVNLKDRLTIELHPDGRHGIVRVDR
VPLASKLVDLPCVMESLKTIDKKTFYKTADICQMLVSTVDGDLYPPVEEPVASTDPKASK
KKDKDKEKKFIWNHGITLPLKNVRKRRFRKTAKKKYIESPDVEKEVKRLLSTDAEAVSTR
WEIIAEDETKEAENQGLDISSPGMSGHRQGHDSLEHDELREIFNDLSSSSEDEDETQHQD
EEDINIIDTEEDLERQLQDKLNESDEQHQENEGTNQLVMGIQKQIDNMKGKLQETQDRAK
RQEDLIMKVENLALKNRFQAVLDELKQKEDREKEQLSSLQEELESLLEK
Sequence of entity 5 (H), FASTA
>7EGH_5 Transcription initiation factor TFIID subunit 8 (chains H)
MADAAATAGAGGSGTRSGSKQSTNPADNYHLARRRTLQVVVSSLLTEAGFESAEKASVET
LTEMLQSYISEIGRSAKSYCEHTARTQPTLSDIVVTLVEMGFNVDTLPAYAKRSQRMVIT
APPVTNQPVTPKALTAGQNRPHPPHIPSHFPEFPDPHTYIKTPTYREPVSDYQVLREKAA
SQRRDVERALTRFMAKTGETQSLFKDDVSTFPLIAARPFTIPYLTALLPSELEMQQMEET
DSSEQDEQTDTENLALHISMEDSGAEKENTSVLQQNPSLSGSRNGEENIIDNPYLRPVKK
PKIRRKKSLS
Sequence of entity 6 (X), FASTA
>7EGH_6 DNA (45-MER) (chains X)
CTATAAAAGGGGGTGGGGGCGCGTTCGTCCTCAGTCGCGATCGAACACTCGAGCCGAGCA
GACGTGCCTACG
Sequence of entity 7 (Y), FASTA
>7EGH_7 DNA (45-MER) (chains Y)
ATCCATGGTCCGTAGGCACGTCTGCTCGGCTCGAGTGTTCGATCGCGACTGAGGACGAAC
GCGCCCCCACCCCCTTTTATAGGCGCCCTTCGAT
Primary citation
Structural insights into preinitiation complex assembly on core promoters. Chen, X., Qi, Y., Wu, Z. et al. Science (2021) 372. DOI 10.1126/science.aba8490 · PubMed
Other PDB entries of the same protein (UniProt P21675 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 5I29 1.21 Å, TAF1(2) bound to a pyrrolopyridone compound
- 7LB1 1.35 Å, Crystal structure of the tandem bromodomain (BD1, BD2) of human TAF1 bound to ZS1-585
- 4YYM 1.5 Å, Crystal structure of TAF1 BD2 Bromodomain bound to a butyryllysine peptide
- 5I1Q 1.5 Å, Second bromodomain of TAF1 bound to a pyrrolopyridone compound
- 7K27 1.5 Å, Crystal structure of the tandem bromodomain (BD1, BD2) of human TAF1 bound to ATR…
- 7JJG 1.6 Å, Crystal structure of the second bromodomain (BD2) of human TAF1 bound to ATR kinase…
- 7K03 1.6 Å, Crystal structure of the tandem bromodomain (BD1 and BD2) of human TAF1 bound to ATR…
- 7T36 1.65 Å, Crystal structure of the tandem bromodomain (BD1 and BD2) of human TAF1 bound to ZS1-322
- 7JSP 1.7 Å, Crystal structure of the second bromodomain (BD2) of human TAF1 bound to ATR kinase…
- 7K3O 1.7 Å, Crystal structure of the unliganded second bromodomain (BD2) of human TAF1
- 7K42 1.7 Å, Crystal structure of the second bromodomain (BD2) of human TAF1 bound to dioxane
- 7LB2 1.7 Å, Crystal structure of the tandem bromodomain (BD1, BD2) of human TAF1 bound to ZS1-589
Browse structure collections
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