7EIB: Type 1 bradykinin receptor

Cryo-EM structure of the type 1 bradykinin receptor in complex with the des-Arg10-kallidin and an Gq protein. Determined by electron microscopy at 3.0 Å resolution. Released 13 Oct 2021.

Method: Electron microscopy
Resolution: 3.0 Å
Organisms: Homo sapiens, Rattus norvegicus, Bos taurus
Chains: 5
Atoms: 7,204
Mol. weight: 161.02 kDa
Released: 13 Oct 2021

Explore 7EIB in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7EIB contains 33 α-helices and 36 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 15 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	31-63	33
α-helix	71-88	18
α-helix	90-97	8
α-helix	107-140	34
α-helix	144-147	4
α-helix	150-167	18
α-helix	170-175	6
β-strand	176-181	6	1
β-strand	186-191	6	1
α-helix	197-203	7
α-helix	204-212	9
α-helix	213-238	26
α-helix	247-278	32
α-helix	284-301	18
α-helix	303-309	7
α-helix	310-314	5
α-helix	317-324	8

Chain B: 10 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	7-32	26
β-strand	34-40	7	2
α-helix	46-56	11
β-strand	184-191	8	2
β-strand	194-201	8	2
α-helix	211-217	7
β-strand	220-226	7	2
α-helix	230-245	16
α-helix	247-249	3
β-strand	253-259	7	2
α-helix	261-270	10
α-helix	275-277	3
α-helix	280-283	4
α-helix	299-319	21
β-strand	326-330	5	2
α-helix	338-357	20

Chain C: 4 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	4-25	22
α-helix	30-33	4
α-helix	34-36	3
β-strand	47-51	5	3
β-strand	58-63	6	4
β-strand	69-74	6	4
β-strand	78-83	6	4
β-strand	89-94	6	4
β-strand	100-105	6	5
β-strand	111-116	6	5
β-strand	121-125	5	5
β-strand	134-139	6	5
β-strand	146-153	8	6
β-strand	156-161	6	6
β-strand	166-170	5	6
β-strand	175-180	6	6
β-strand	187-192	6	7
β-strand	198-203	6	7
β-strand	208-212	5	7
β-strand	218-222	5	7
β-strand	229-234	6	8
β-strand	240-245	6	8
β-strand	249-254	6	8
β-strand	259-264	6	8
α-helix	272	1
β-strand	273-278	6	9
β-strand	284-289	6	9
β-strand	294-298	5	9
β-strand	303-307	5	9
β-strand	315-320	6	3
β-strand	327-331	5	3
β-strand	336-339	4	3

Chain D: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	2-3	2

Chain E: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	12-23	12
α-helix	30-43	14
α-helix	53-55	3

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
B1 bradykinin receptor	A	protein	613	Homo sapiens	P46663 (AlphaFold model)
Lys-arg-pro-pro-gly-phe-ser-pro-phe	D	protein	9	Homo sapiens	P01042 (AlphaFold model)
G subunit q (Gi1-Gq chimeric)	B	protein	361	Homo sapiens
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	C	protein	377	Rattus norvegicus	P54311 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	E	protein	71	Bos taurus	P63212 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>7EIB_1 B1 bradykinin receptor (chains A)
ADLEDNWETLNDNLKVIEKADNAAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKD
FRHGFDILVGQIDDALKLANEGKVKEAQAAAEQLKTTRNAYIQKYLASSWPPLELQSSNQ
SQLFPQNATACDNAPEAWDLLHRVLPTFIISICFFGLLGNLFVLLVFLLPRRQLNVAEIY
LANLAASDLVFVLGLPFWAENIWNQFNWPFGALLCRVINGVIKANLFISIWLVVAISQDR
YRVLVHPMASRRQQRRRQARVTCVLIWVVGGLLSIPTFLLRSIQAVPDLNITACILLLPH
EAWHFARIVELNILGFLLPLAAIVFFNYHILASLRTREEVSRTRCGGRKDSKTTALILTL
VVAFLVCWAPYHFFAFLEFLFQVQAVRGCFWEDFIDLGLQLANFFAFTNSSLNPVIYVFV
GRLFRTKVWELYKQCTPKSLAPISSSHRKEIFQLFVFTLEDFVGDWEQTAAYNLDQVLEQ
GGVSSLLQNLAVSVTPIQRIVRSGENALKIDIHVIIPYEGLSADQMAQIEEVFKVVYPVD
DHHFKVILPYGTLVIDGVTPNMLNYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLIT
PDGSMLFRVTINS

Sequence of entity 2 (D), FASTA

>7EIB_2 LYS-ARG-PRO-PRO-GLY-PHE-SER-PRO-PHE (chains D)
KRPPGFSPF

Sequence of entity 3 (B), FASTA

>7EIB_3 G subunit q (Gi1-Gq chimeric) (chains B)
MGCTLSAEDKAAVERSKMIEKQLQKDKQVYRRTLRLLLLGADNSGKSTIVKQMRIYHVNG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQEALNDF
KSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPR
VTRAKYFIRKEFVDISTASGDGRHICYPHFTCSVDTENARRIFNDCKDIILQMNLREYNL
V

Sequence of entity 4 (C), FASTA

>7EIB_4 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains C)
MHHHHHHGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRR
TLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSG
NYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALW
DIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDI
NAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGY
DDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWNGSSGGGGSG
GGGSSGVSGWRLFKKIS

Sequence of entity 5 (E), FASTA

>7EIB_5 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains E)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Primary citation

Molecular basis for kinin selectivity and activation of the human bradykinin receptors. Yin, Y.L., Ye, C., Zhou, F. et al. Nat Struct Mol Biol (2021) 28:755-761. DOI 10.1038/s41594-021-00645-y · PubMed

Other PDB entries of the same protein (UniProt P46663 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9LFA 3.5 Å, Cryo-EM structure of human bradykinin receptor B1R bound to antagonist ELN441958
9LFC 3.6 Å, Cryo-EM structure of human bradykinin receptor B1R bound to antagonist R715

Browse structure collections

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