Overall structure of Erastin-bound xCT-4F2hc complex. Determined by electron microscopy at 3.4 Å resolution. Released 6 Apr 2022.
Explore 7EPZ in 3D Show helices and sheets RCSB PDB PDBe
7EPZ contains 38 α-helices and 22 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 179-206 | 28 | |
| α-helix | 212-214 | 3 | |
| β-strand | 225-228 | 4 | 1 |
| α-helix | 232-235 | 4 | |
| α-helix | 244-247 | 4 | |
| α-helix | 249-255 | 7 | |
| β-strand | 261-262 | 2 | 1 |
| β-strand | 266-267 | 2 | 2 |
| β-strand | 277-281 | 5 | 2 |
| α-helix | 282 | 1 | |
| α-helix | 288-300 | 13 | |
| β-strand | 306-308 | 3 | 1 |
| α-helix | 325-341 | 17 | |
| β-strand | 345-349 | 5 | 1 |
| α-helix | 351-353 | 3 | |
| α-helix | 357-371 | 15 | |
| β-strand | 376-380 | 5 | 1 |
| α-helix | 386-392 | 7 | |
| β-strand | 400-402 | 3 | 1 |
| α-helix | 413-427 | 15 | |
| β-strand | 432-434 | 3 | 3 |
| α-helix | 451-459 | 9 | |
| β-strand | 463-465 | 3 | 3 |
| β-strand | 466-468 | 3 | 1 |
| α-helix | 488-490 | 3 | |
| α-helix | 520-533 | 14 | |
| β-strand | 541-544 | 4 | 4 |
| β-strand | 551-557 | 7 | 4 |
| β-strand | 563-569 | 7 | 4 |
| β-strand | 575-576 | 2 | 5 |
| β-strand | 593-594 | 2 | 6 |
| β-strand | 599 | 1 | 4 |
| β-strand | 610-611 | 2 | 6 |
| β-strand | 616-617 | 2 | 5 |
| β-strand | 622-627 | 6 | 4 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 46-57 | 12 | |
| α-helix | 61-71 | 11 | |
| α-helix | 76-104 | 29 | |
| α-helix | 111-114 | 4 | |
| α-helix | 122-129 | 8 | |
| α-helix | 130-134 | 5 | |
| α-helix | 135-148 | 14 | |
| α-helix | 152-154 | 3 | |
| α-helix | 162-181 | 20 | |
| α-helix | 184-206 | 23 | |
| α-helix | 235-243 | 9 | |
| α-helix | 244-246 | 3 | |
| α-helix | 249-251 | 3 | |
| α-helix | 265-289 | 25 | |
| α-helix | 303-308 | 6 | |
| α-helix | 319-332 | 14 | |
| α-helix | 339-346 | 8 | |
| β-strand | 358-359 | 2 | 7 |
| α-helix | 366-381 | 16 | |
| α-helix | 385-412 | 28 | |
| α-helix | 426-441 | 16 | |
| α-helix | 447-458 | 12 | |
| α-helix | 461-463 | 3 | |
| α-helix | 478-492 | 15 | |
| β-strand | 494-495 | 2 | 7 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| 4F2 cell-surface antigen heavy chain | A | protein | 647 | Homo sapiens | P08195 (AlphaFold model) |
| Cystine/glutamate transporter | B | protein | 521 | Homo sapiens | Q9UPY5 (AlphaFold model) |
>7EPZ_1 4F2 cell-surface antigen heavy chain (chains A) MAHHHHHHHHHHSGRELQPPEASIAVVSIPRQLPGSHSEAGVQGLSAGDDSETGSDCVTQ AGLQLLASSDPPALASKNAEVTVETGFHHVSQADIEFLTSIDPTASASGSAGITGTMSQD TEVDMKEVELNELEPEKQPMNAASGAAMSLAGAEKNGLVKIKVAEDEAEAAAAAKFTGLS KEELLKVAGSPGWVRTRWALLLLFWLGWLGMLAGAVVIIVRAPRCRELPAQKWWHTGALY RIGDLQAFQGHGAGNLAGLKGRLDYLSSLKVKGLVLGPIHKNQKDDVAQTDLLQIDPNFG SKEDFDSLLQSAKKKSIRVILDLTPNYRGENSWFSTQVDTVATKVKDALEFWLQAGVDGF QVRDIENLKDASSFLAEWQNITKGFSEDRLLIAGTNSSDLQQILSLLESNKDLLLTSSYL SDSGSTGEHTKSLVTQYLNATGNRWCSWSLSQARLLTSFLPAQLLRLYQLMLFTLPGTPV FSYGDEIGLDAAALPGQPMEAPVMLWDESSFPDIPGAVSANMTVKGQSEDPGSLLSLFRR LSDQRSKERSLLHGDFHAFSAGPGLFSYIRHWDQNERFLVVLNFGDVGLSAGLQASDLPA SASLPAKADLLLSTQPGREEGSPLELERLKLEPHEGLLLRFPYAALE
>7EPZ_2 Cystine/glutamate transporter (chains B) MADYKDDDDKSGPDEVDASGRVRKPVVSTISKGGYLQGNVNGRLPSLGNKEPPGQEKVQL KRKVTLLRGVSIIIGTIIGAGIFISPKGVLQNTGSVGMSLTIWTVCGVLSLFGALSYAEL GTTIKKSGGHYTYILEVFGPLPAFVRVWVELLIIRPAATAVISLAFGRYILEPFFIQCEI PELAIKLITAVGITVVMVLNSMSVSWSARIQIFLTFCKLTAILIIIVPGVMQLIKGQTQN FKDAFSGRDSSITRLPLAFYYGMYAYAGWFYLNFVTEEVENPEKTIPLAICISMAIVTIG YVLTNVAYFTTINAEELLLSNAVAVTFSERLLGNFSLAVPIFVALSCFGSMNGGVFAVSR LFYVASREGHLPEILSMIHVRKHTPLPAVIVLHPLTMIMLFSGDLDSLLNFLSFARWLFI GLAVAGLIYLRYKCPDMHRPFKVPLFIPALFSFTCLFMVALSLYSDPFSTGIGFVITLTG VPAYYLFIIWDKKPRWFRIMSEKITRTLQIILEVVPEEDKL
| ID | Name | Formula | Copies |
|---|---|---|---|
| PX8 | 1,2-distearoyl-sn-glycero-3-phosphate | C39 H76 O8 P | 2 |
| J9O | 2-[(1S)-1-[4-[2-(4-chloranylphenoxy)ethanoyl]piperazin-1-yl]ethyl]-3-(2-ethoxyp… | C30 H31 Cl N4 O4 | 1 |
The structure of erastin-bound xCT-4F2hc complex reveals molecular mechanisms underlying erastin-induced ferroptosis. Yan, R., Xie, E., Li, Y. et al. Cell Res (2022) 32:687-690. DOI 10.1038/s41422-022-00642-w · PubMed
Other PDB entries of the same protein (UniProt P08195 (AlphaFold model), which also has an AlphaFold model), best resolution first:
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