7ETW: Scap/Insig complex in the present of digitonin

Cryo-EM structure of Scap/Insig complex in the present of digitonin. Determined by electron microscopy at 4.1 Å resolution. Released 23 Jun 2021.

Method
Electron microscopy
Resolution
4.1 Å
Organism
Homo sapiens
Chains
2
Atoms
4,952
Mol. weight
111.95 kDa
Ligands
AJP
Released
23 Jun 2021

Explore 7ETW in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7ETW contains 30 α-helices and 12 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 9 helices, 0 β-strands

ElementResiduesLengthSheet
α-helix19-4729
α-helix59-613
α-helix62-665
α-helix73-9422
α-helix105-12016
α-helix130-14617
α-helix152-17221
α-helix185-1906
α-helix192-21120
Chain B: 21 helices, 12 β-strands
ElementResiduesLengthSheet
α-helix12-2211
α-helix26-4015
β-strand9311
β-strand95-10172
α-helix113-13018
β-strand134-13633
β-strand139-14133
α-helix143-1453
α-helix155-1606
β-strand171-17224
α-helix174-1785
α-helix182-1854
α-helix192-1965
α-helix210-2145
β-strand232-23652
β-strand237-23824
β-strand23911
α-helix245-25814
β-strand272-27545
α-helix283-2853
α-helix286-30419
α-helix313-33523
α-helix349-3557
α-helix359-36911
α-helix377-40832
α-helix412-43221
α-helix435-44915
α-helix515-53218
α-helix535-5373
α-helix633-6386
β-strand648-65145
β-strand653-65422
β-strand658-65922

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Insulin-induced gene 2 proteinAprotein225Homo sapiensQ9Y5U4 (AlphaFold model)
Sterol regulatory element-binding protein cleavage-activating proteinBprotein735Homo sapiensQ12770 (AlphaFold model)
Sequence of entity 1 (A), FASTA
>7ETW_1 Insulin-induced gene 2 protein (chains A)
MAEGETESPGPKKSGPYISSVTSQSVNLMIRGVVLFFIGVFLALVLNLLQIQRNVTLFPP
DVIASIFSSAWWVPPCCGTASAVIGLLYPSIDRHLGEPHKFKREWSSVMRCVAVFVGINH
ASAKVDFDNNIQLSLTLAALSIGLWWTFDRSRSGFGLGVGIAFLATVVTQLLVYNGVYQY
TSPDFLYVRSWLPCIFFAGGITMGNIGRQLAMYESKVIAEKSHQE
Sequence of entity 2 (B), FASTA
>7ETW_2 Sterol regulatory element-binding protein cleavage-activating protein (chains B)
MTLTERLREKISRAFYNHGLLCASYPIPIILFTGFCILACCYPLLKLPLPGTGPVEFTTP
VKDYSPPPVDSDRKQGEPTEQPEWYVGAPVAYVQQIFVKSSVFPWHKNLLAVDVFRSPLS
RAFQLVEEIRNHVLRDSSGIRSLEELCLQVTDLLPGLRKLRNLLPEHGCLLLSPGNFWQN
DWERFHADPDIIGTIHQHEPKTLQTSATLKDLLFGVPGKYSGVSLYTRKRMVSYTITLVF
QHYHAKFLGSLRARLMLLHPSPNCSLRAESLVHVHFKEEIGVAELIPLVTTYIILFAYIY
FSTRKIDMVKSKWGLALAAVVTVLSSLLMSVGLCTLFGLTPTLNGGEIFPYLVVVIGLEN
VLVLTKSVVSTPVDLEVKLRIAQGLSSESWSIMKNMATELGIILIGYFTLVPAIQEFCLF
AVVGLVSDFFLQMLFFTTVLSIDIRRMELADLNKRLPPEACLPSAKPVGQPTRYERQLAV
RPSTPHTITLQPSSFRNLRLPKRLRVVYFLARTRLAQRLIMAGTVVWIGILVYTDPAGLR
NYLAAQVTEQSPLGEGALAPMPVPSGMLPPSHPDPAFSIFPPDAPKLPENQTSPGESPER
GGPAEVVHDSPVPEVTWGPEDEELWRKLSFRHWPTLFSYYNITLAKRYISLLPVIPVTLR
LNPREALEGRHPQDGRSAWPPPGPIPAGHWEAGPKGPGGVQAHGDVTLYKVAALGLATGI
VLVLLLLCLYRVLCP

Ligands and cofactors

IDNameFormulaCopies
AJPDigitoninC56 H92 O294

Primary citation

Structural basis for sterol sensing by Scap and Insig. Yan, R., Cao, P., Song, W. et al. Cell Rep (2021) 35:109299. DOI 10.1016/j.celrep.2021.109299

Other PDB entries of the same protein (UniProt Q9Y5U4 (AlphaFold model), which also has an AlphaFold model), best resolution first:

Browse structure collections

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