Structure of C9orf72:SMCR8:WDR41 in complex with ARF1. Determined by electron microscopy at 3.94 Å resolution. Released 23 Jun 2021.
Explore 7MGE in 3D Show helices and sheets RCSB PDB PDBe
7MGE contains 47 α-helices and 71 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 35-39 | 5 | 1 |
| β-strand | 56 | 1 | 2 |
| β-strand | 66 | 1 | 3 |
| β-strand | 70 | 1 | 2 |
| β-strand | 80 | 1 | 3 |
| β-strand | 87-91 | 5 | 4 |
| β-strand | 107-110 | 4 | 4 |
| β-strand | 115-117 | 3 | 4 |
| β-strand | 128-130 | 3 | 4 |
| β-strand | 140-142 | 3 | 5 |
| β-strand | 147-149 | 3 | 5 |
| β-strand | 156-158 | 3 | 5 |
| β-strand | 164-167 | 4 | 5 |
| β-strand | 180-182 | 3 | 6 |
| β-strand | 186-188 | 3 | 6 |
| β-strand | 189-191 | 3 | 7 |
| β-strand | 194-201 | 8 | 7 |
| β-strand | 211-218 | 8 | 7 |
| β-strand | 225 | 1 | 8 |
| β-strand | 230-231 | 2 | 9 |
| β-strand | 235-239 | 5 | 9 |
| β-strand | 240 | 1 | 8 |
| β-strand | 245-249 | 5 | 9 |
| β-strand | 258-259 | 2 | 9 |
| β-strand | 289-291 | 3 | 10 |
| β-strand | 296-300 | 5 | 10 |
| β-strand | 303-307 | 5 | 10 |
| β-strand | 314-318 | 5 | 10 |
| β-strand | 326-332 | 7 | 11 |
| β-strand | 336-341 | 6 | 11 |
| β-strand | 345-350 | 6 | 11 |
| β-strand | 396-402 | 7 | 11 |
| β-strand | 411-413 | 3 | 1 |
| β-strand | 419-421 | 3 | 1 |
| β-strand | 428-432 | 5 | 1 |
| α-helix | 435-442 | 8 | |
| α-helix | 446-452 | 7 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 59 | 1 | 12 |
| α-helix | 99-104 | 6 | |
| β-strand | 119 | 1 | 12 |
| β-strand | 120 | 1 | 13 |
| β-strand | 131-138 | 8 | 12 |
| β-strand | 152-158 | 7 | 12 |
| α-helix | 162-208 | 47 | |
| α-helix | 209-213 | 5 | |
| α-helix | 214-218 | 5 | |
| α-helix | 226-251 | 26 | |
| α-helix | 331-343 | 13 | |
| α-helix | 349-362 | 14 | |
| α-helix | 368-370 | 3 | |
| α-helix | 708-713 | 6 | |
| α-helix | 721-729 | 9 | |
| β-strand | 732-736 | 5 | 14 |
| α-helix | 740-749 | 10 | |
| α-helix | 750-753 | 4 | |
| β-strand | 764 | 1 | 15 |
| α-helix | 775-777 | 3 | |
| β-strand | 782 | 1 | 15 |
| β-strand | 784-785 | 2 | 14 |
| α-helix | 797-800 | 4 | |
| α-helix | 801-803 | 3 | |
| β-strand | 807-811 | 5 | 14 |
| β-strand | 816 | 1 | 14 |
| α-helix | 826-830 | 5 | |
| α-helix | 841-862 | 22 | |
| α-helix | 865-868 | 4 | |
| α-helix | 877-890 | 14 | |
| α-helix | 896-899 | 4 | |
| α-helix | 900-914 | 15 | |
| β-strand | 918 | 1 | 16 |
| β-strand | 922 | 1 | 16 |
| β-strand | 926 | 1 | 1 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 15-17 | 3 | 17 |
| β-strand | 27-29 | 3 | 17 |
| β-strand | 45-47 | 3 | 17 |
| α-helix | 56-67 | 12 | |
| β-strand | 81-85 | 5 | 17 |
| β-strand | 86 | 1 | 13 |
| β-strand | 92-98 | 7 | 17 |
| β-strand | 99-100 | 2 | 18 |
| β-strand | 109-110 | 2 | 18 |
| β-strand | 113-117 | 5 | 17 |
| α-helix | 125-127 | 3 | |
| α-helix | 128-147 | 20 | |
| α-helix | 174-178 | 5 | |
| α-helix | 181-189 | 9 | |
| α-helix | 190-192 | 3 | |
| α-helix | 214-229 | 16 | |
| β-strand | 234-237 | 4 | 19 |
| α-helix | 242-251 | 10 | |
| α-helix | 252-254 | 3 | |
| α-helix | 257-260 | 4 | |
| β-strand | 263-264 | 2 | 19 |
| β-strand | 280-282 | 3 | 19 |
| α-helix | 295-299 | 5 | |
| β-strand | 307-309 | 3 | 19 |
| β-strand | 316 | 1 | 19 |
| α-helix | 376-384 | 9 | |
| α-helix | 393-418 | 26 | |
| α-helix | 431-436 | 6 | |
| α-helix | 442-453 | 12 | |
| α-helix | 459-464 | 6 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 17-20 | 4 | 20 |
| β-strand | 23-24 | 2 | 20 |
| α-helix | 30-38 | 9 | |
| α-helix | 46-48 | 3 | |
| β-strand | 52-58 | 7 | 20 |
| β-strand | 61-67 | 7 | 20 |
| α-helix | 75-81 | 7 | |
| β-strand | 87-92 | 6 | 20 |
| α-helix | 100-111 | 12 | |
| α-helix | 114-116 | 3 | |
| β-strand | 120-125 | 6 | 20 |
| α-helix | 136-143 | 8 | |
| α-helix | 145-147 | 3 | |
| β-strand | 159 | 1 | 21 |
| β-strand | 164 | 1 | 21 |
| α-helix | 166-177 | 12 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| WD repeat-containing protein 41 | A | protein | 459 | Homo sapiens | Q9HAD4 (AlphaFold model) |
| Guanine nucleotide exchange protein SMCR8 | B | protein | 937 | Homo sapiens | Q8TEV9 (AlphaFold model) |
| Guanine nucleotide exchange C9orf72 | C | protein | 481 | Homo sapiens | Q96LT7 (AlphaFold model) |
| ADP-ribosylation factor 1 | E | protein | 166 | Homo sapiens | P84077 (AlphaFold model) |
>7MGE_1 WD repeat-containing protein 41 (chains A) MLRWLIGGGREPQGLAEKSPLQTIGEEQTQNPYTELLVLKAHHDIVRFLVQLDDYRFASA GDDGIVVVWNAQTGEKLLELNGHTQKITAIITFPSLESCEEKNQLILTASADRTVIVWDG DTTRQVQRISCFQSTVKCLTVLQRLDVWLSGGNDLCVWNRKLDLLCKTSHLSDTGISALV EIPKNCVVAAVGKELIIFRLVAPTEGSLEWDILEVKRLLDHQDNILSLINVNDLSFVTGS HVGELIIWDALDWTMQAYERNFWDPSPQLDTQQEIKLCQKSNDISIHHFTCDEENVFAAV GRGLYVYSLQMKRVIACQKTAHDSNVLHVARLPNRQLISCSEDGSVRIWELREKQQLAAE PVPTGFFNMWGFGRVSKQASQPVKKQQENATSCSLELIGDLIGHSSSVEMFLYFEDHGLV TCSADHLIILWKNGERESGLRSLRLFQKLEENGDLYLAV
>7MGE_2 Guanine nucleotide exchange protein SMCR8 (chains B) MISAPDVVAFTKEEEYEEEPYNEPALPEEYSVPLFPFASQGANPWSKLSGAKFSRDFILI SEFSEQVGPQPLLTIPNDTKVFGTFDLNYFSLRIMSVDYQASFVGHPPGSAYPKLNFVED SKVVLGDSKEGAFAYVHHLTLYDLEARGFVRPFCMAYISADQHKIMQQFQELSAEFSRAS ECLKTGNRKAFAGELEKKLKDLDYTRTVLHTETEIQKKANDKGFYSSQAIEKANELASVE KSIIEHQDLLKQIRSYPHRKLKGHDLCPGEMEHIQDQASQASTTSNPDESADTDLYTCRP AYTPKLIKAKSTKCFDKKLKTLEELCDTEYFTQTLAQLSHIEHMFRGDLCYLLTSQIDRA LLKQQHITNFLFEDFVEVDDRMVEKQESIPSKPSQDRPPSSSLEECPIPKVLISVGSYKS SVESVLIKMEQELGDEEYKEVEVTELSSFDPQENLDYLDMDMKGSISSGESIEVLGTEKS TSVLSKSDSQASLTVPLSPQVVRSKAVSHRTISEDSIEVLSTCPSEALIPDDFKASYPSA INEEESYPDGNEGAIRFQASISPPELGETEEGSIENTPSQIDSSCCIGKESDGQLVLPST PAHTHSDEDGVVSSPPQRHRQKDQGFRVDFSVENANPSSRDNSCEGFPAYELDPSHLLAS RDISKTSLDNYSDTTSYVSSVASTSSDRIPSAYPAGLSSDRHKKRAGQNALKFIRQYPFA HPAIYSLLSGRTLVVLGEDEAIVRKLVTALAIFVPSYGCYAKPVKHWASSPLHIMDFQKW KLIGLQRVASPAGAGTLHALSRYSRYTSILDLDNKTLRCPLYRGTLVPRLADHRTQIKRG STYYLHVQSMLTQLCSKAFLYTFCHHLHLPTHDKETEELVASRQMSFLKLTLGLVNEDVR VVQYLAELLKLHYMQESPGTSHPMLRFDYVPSFLYKI
>7MGE_3 Guanine nucleotide exchange C9orf72 (chains C) MSTLCPPPSPAVAKTEIALSGKSPLLAATFAYWDNILGPRVRHIWAPKTEQVLLSDGEIT FLANHTLNGEILRNAESGAIDVKFFVLSEKGVIIVSLIFDGNWNGDRSTYGLSIILPQTE LSFYLPLHRVCVDRLTHIIRKGRIWMHKERQENVQKIILEGTERMEDQGQSIIPMLTGEV IPVMELLSSMKSHSVPEEIDIADTVLNDDDIGDSCHEGFLLNAISSHLQTCGCSVVVGSS AEKVNKIVRTLCLFLTPAERKCSRLCEAESSFKYESGLFVQGLLKDSTGSFVLPFRQVMY APYPTTHIDVDVNTVKQMPPCHEHIYNQRRYMRSELTAFWRATSEEDMAQDTIIYTDESF TPDLNIFQDVLHRDTLVKAFLDQVFQLKPGLSLRSTFLAQFLLVLHRKALTLIKYIEDDT QKGKKPFKSLRNLKIDLDLTAEGDLNIIMALAEKIKPGLHSFIFGRPFYTSVQERDVLMT F
>7MGE_4 ADP-ribosylation factor 1 (chains E) ATEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKI RPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAM NAAEITDKLGLHSLRHRNWYIQATCATSGDGLYEGLDWLSNQLRNQ
| ID | Name | Formula | Copies |
|---|---|---|---|
| GDP | Guanosine-5'-diphosphate | C10 H15 N5 O11 P2 | 1 |
| MG | Magnesium ion | Mg | 1 |
| BEF | Beryllium trifluoride ion | Be F3 | 1 |
Structural basis for the ARF GAP activity and specificity of the C9orf72 complex. Su, M.Y., Fromm, S.A., Remis, J. et al. Nat Commun (2021) 12:3786-3786. DOI 10.1038/s41467-021-24081-0 · PubMed
Other PDB entries of the same protein (UniProt Q9HAD4 (AlphaFold model), which also has an AlphaFold model), best resolution first:
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