7MM4: HCV NS3/4A protease

Crystal structure of HCV NS3/4A protease in complex with NR01-115. Determined by X-ray diffraction at 1.89 Å resolution. Released 16 Mar 2022.

Method: X-ray diffraction
Resolution: 1.89 Å
Organism: Hepacivirus C
Chains: 1
Atoms: 1,705
Mol. weight: 22.87 kDa
Ligands: ZKA, ZN
Released: 16 Mar 2022

Explore 7MM4 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7MM4 contains 10 α-helices and 22 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 10 helices, 22 β-strands

Element	Residues	Length	Sheet
β-strand	992	1	1
β-strand	993-999	7	2
β-strand	1006-1009	4	2
α-helix	1013-1022	10
β-strand	1024	1	3
β-strand	1031	1	4
β-strand	1033-1037	5	2
β-strand	1042-1048	7	2
β-strand	1051-1055	5	2
α-helix	1056-1059	4
β-strand	1064	1	1
β-strand	1066	1	3
β-strand	1071	1	1
β-strand	1075-1077	3	2
α-helix	1078-1080	3
β-strand	1082-1086	5	2
α-helix	1087-1088	2
β-strand	1091	1	4
α-helix	1093	1
β-strand	1094	1	2
α-helix	1095	1
β-strand	1096	1	5
α-helix	1097	1
β-strand	1103-1107	5	5
β-strand	1113-1118	6	5
β-strand	1123-1131	9	5
α-helix	1132-1134	3
α-helix	1141	1
β-strand	1142-1144	3	5
β-strand	1150-1160	11	5
β-strand	1163-1171	9	5
α-helix	1172-1178	7

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
NS3 protease	A	protein	200	Hepacivirus C	P26664 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>7MM4_1 NS3 protease (chains A)
HMASMKKKGSVVIVGRINLSGDTAYAQQTRGEEGCQETSQTGRDKNQVEGEVQIVSTATQ
TFLATSINGVLWTVYHGAGTRTIASPKGPVTQMYTNVDKDLVGWQAPQGSRSLTPCTCGS
SDLYLVTRHADVIPVRRRGDSRGSLLSPRPISYLKGSSGGPLLCPAGHAVGIFRAAVSTR
GVAKAVDFIPVESLETTMRS

Ligands and cofactors

ID	Name	Formula	Copies
ZKA	(1-methylcyclopropyl)methyl {(2R,4S,6S,12Z,13aS,14aR,16aS)-2-[(7-methoxy-3-meth…	C38 H50 N6 O9 S	1
ZN	Zinc ion	Zn	1

Water and common crystallization additives (SO4, EDO) are not listed.

Primary citation

Deciphering the Molecular Mechanism of HCV Protease Inhibitor Fluorination as a General Approach to Avoid Drug Resistance. Zephyr, J., Nageswara Rao, D., Vo, S.V. et al. J Mol Biol (2022) 434:167503-167503. DOI 10.1016/j.jmb.2022.167503 · PubMed

Other PDB entries of the same protein (UniProt P26664 (AlphaFold model), which also has an AlphaFold model), best resolution first:

3RC4 1.5 Å, Molecular mechanisms of viral and host-cell substrate recognition by HCV NS3/4A protease
3HKW 1.55 Å, HCV NS5B genotype 1a in complex with 1,5 benzodiazepine inhibitor 6
7MME 1.56 Å, Crystal structure of HCV NS3/4A D168A protease in complex with JZ01-15
3RC5 1.6 Å, Molecular mechanisms of viral and host-cell substrate recognition by HCV NS3/4A protease
7MMA 1.65 Å, Crystal structure of HCV NS3/4A protease in complex with NR01-145
7MML 1.7 Å, Crystal structure of HCV NS3/4A D168A protease in complex with NR01-145
6P6L 1.73 Å, HCV NS3/4A protease domain of genotype 1a in complex with glecaprevir
6P6R 1.75 Å, HCV NS3/4A protease domain of genotype 1a3a chimera in complex with glecaprevir
7MM3 1.78 Å, Crystal structure of HCV NS3/4A protease in complex with NR01-127
5VOJ 1.8 Å, Crystal structure of HCV NS3/4A protease in complex with JZ01-15, an analogue of…
3QGI 1.8 Å, Crystal structure of the hepatitis C virus NS5B RNA-dependent RNA polymerase genotype 1a…
5VP9 1.86 Å, Crystal structure of HCV NS3/4A protease in complex with AM-07, an analogue of 5172-mcP1P3

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