7NJ1: Human Separase-Securin complex

CryoEM structure of the human Separase-Securin complex. Determined by electron microscopy at 2.9 Å resolution. Released 4 Aug 2021.

Method
Electron microscopy
Resolution
2.9 Å
Organism
Homo sapiens
Chains
2
Atoms
11,273
Mol. weight
259.66 kDa
Released
4 Aug 2021

Explore 7NJ1 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7NJ1 contains 76 α-helices and 18 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 73 helices, 18 β-strands

ElementResiduesLengthSheet
α-helix245-26420
α-helix269-28113
α-helix287-30317
α-helix309-32517
α-helix332-34817
α-helix357-37923
α-helix381-3855
α-helix387-41428
α-helix420-44021
α-helix446-46823
α-helix473-48917
α-helix500-51617
α-helix520-53314
α-helix543-55816
α-helix562-5665
α-helix569-5724
α-helix578-59417
α-helix600-61314
α-helix619-63618
α-helix649-66214
α-helix670-70132
α-helix729-7368
α-helix740-75819
α-helix769-78517
α-helix789-80517
α-helix809-82517
α-helix830-84415
α-helix851-87020
α-helix874-88512
α-helix888-8914
α-helix895-91218
α-helix921-9288
α-helix935-95319
α-helix976-100025
α-helix1004-102118
α-helix1024-104017
α-helix1044-106118
α-helix1148-11525
α-helix1154-117320
α-helix1178-120629
α-helix1218-123114
α-helix1233-12364
α-helix1247-125610
α-helix1263-127715
α-helix1574-158815
α-helix1595-160713
α-helix1613-162210
α-helix1626-164621
α-helix1668-167710
α-helix1689-170012
α-helix1702-17032
β-strand1706-171491
β-strand1724-173071
α-helix1735-17362
β-strand1737-174261
α-helix1751-176717
α-helix1773-179321
α-helix1794-17996
α-helix1804-18063
α-helix1815-182915
α-helix1837-18426
α-helix1846-18483
α-helix1851-186111
α-helix1867-187913
β-strand1890-189451
α-helix1904-19063
β-strand1915-191731
α-helix1921-193313
α-helix1937-19404
β-strand194312
β-strand1948-195251
α-helix1959-197113
β-strand197511
β-strand1978-197921
α-helix1982-19843
α-helix1985-199410
β-strand1997-200151
α-helix2012-20165
β-strand2023-202751
β-strand203513
α-helix2041-20422
β-strand204313
α-helix2045-20506
β-strand2056-205941
α-helix2067-208317
β-strand208814
α-helix2089-20957
α-helix2096-20983
β-strand2111-211441
β-strand211814
β-strand211912
Chain B: 3 helices, 0 β-strands
ElementResiduesLengthSheet
α-helix112-1165
α-helix122-1254
α-helix155-1573

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
SeparinAprotein2160Homo sapiensQ14674 (AlphaFold model)
SecurinBprotein202Homo sapiensO95997 (AlphaFold model)
Sequence of entity 1 (A), FASTA
>7NJ1_1 Separin (chains A)
MRSFKRVNFGTLLSSQKEAEELLPDLKEFLSNPPAGFPSSRSDAERRQACDAILRACNQQ
LTAKLACPRHLGSLLELAELACDGYLVSTPQRPPLYLERILFVLLRNAAAQGSPEVTLRL
AQPLHACLVQCSREAAPQDYEAVARGSFSLLWKGAEALLERRAAFAARLKALSFLVLLED
ESTPCEVPHFASPTACRAVAAHQLFDASGHGLNEADADFLDDLLSRHVIRALVGERGSSS
GLLSPQRALCLLELTLEHCRRFCWSRHHDKAISAVEKAHSYLRNTNLAPSLQLCQLGVKL
LQVGEEGPQAVAKLLIKASAVLSKSMEAPSPPLRALYESCQFFLSGLERGTKRRYRLDAI
LSLFAFLGGYCSLLQQLRDDGVYGGSSKQQQSFLQMYFQGLHLYTVVVYDFAQGCQIVDL
ADLTQLVDSCKSTVVWMLEALEGLSGQELTDHMGMTASYTSNLAYSFYSHKLYAEACAIS
EPLCQHLGLVKPGTYPEVPPEKLHRCFRLQVESLKKLGKQAQGCKMVILWLAALQPCSPE
HMAEPVTFWVRVKMDAARAGDKELQLKTLRDSLSGWDPETLALLLREELQAYKAVRADTG
QERFNIICDLLELSPEETPAGAWARATHLVELAQVLCYHDFTQQTNCSALDAIREALQLL
DSVRPEAQARDQLLDDKAQALLWLYICTLEAKIQEGIERDRRAQAPGNLEEFEVNDLNYE
DKLQEDRFLYSNIAFNLAADAAQSKCLDQALALWKELLTKGQAPAVRCLQQTAASLQILA
ALYQLVAKPMQALEVLLLLRIVSERLKDHSKAAGSSCHITQLLLTLGCPSYAQLHLEEAA
SSLKHLDQTTDTYLLLSLTCDLLRSQLYWTHQKVTKGVSLLLSVLRDPALQKSSKAWYLL
RVQVLQLVAAYLSLPSNNLSHSLWEQLCAQGWQTPEIALIDSHKLLRSIILLLMGSDILS
TQKAAVETSFLDYGENLVQKWQVLSEVLSCSEKLVCHLGRLGSVSEAKAFCLEALKLTTK
LQIPRQCALFLVLKGELELARNDIDLCQSDLQQVLFLLESCTEFGGVTQHLDSVKKVHLQ
KGKQQAQVPCPPQLPEEELFLRGPALELVATVAKEPGPIAPSTNSSPVLKTKPQPIPNFL
SHSPTCDCSLCASPVLTAVCLRWVLVTAGVRLAMGHQAQGLDLLQVVLKGCPEAAERLTQ
ALQASLNHKTPPSLVPSLLDEILAQAYTLLALEGLNQPSNESLQKVLQSGLKFVAARIPH
LEPWRASLLLIWALTKLGGLSCCTTQLFASSWGWQPPLIKSVPGSEPSKTQGQKRSGRGR
QKLASAPLSLNNTSQKGLEGRGLPCTPKPPDRIRQAGPHVPFTVFEEVCPTESKPEVPQA
PRVQQRVQTRLKVNFSDDSDLEDPVSAEAWLAEEPKRRGTASRGRGRARKGLSLKTDAVV
APGSAPGNPGLNGRSRRAKKVASRHCEERRPQRASDQARPGPEIMRTIPEEELTDNWRKM
SFEILRGSDGEDSASGGKTPAPGPEAASGEWELLRLDSSKKKLPSPCPDKESDKDLGPRL
QLPSAPVATGLSTLDSICDSLSVAFRGISHCPPSGLYAHLCRFLALCLGHRDPYATAFLV
TESVSITCRHQLLTHLHRQLSKAQKHRGSLEIADQLQGLSLQEMPGDVPLARIQRLFSFR
ALESGHFPQPEKESFQERLALIPSGVTVCVLALATLQPGTVGNTLLLTRLEKDSPPVSVQ
IPTGQNKLHLRSVLNEFDAIQKAQKENSSCTDKREWWTGRLALDHRMEVLIASLEKSVLG
CWKGLLLPSSEEPGPAQEASRLQELLQDCGWKYPDRTLLKIMLSGAGALTPQDIQALAYG
LCPTQPERAQELLNEAVGRLQGLTVPSNSHLVLVLDKDLQKLPWESMPSLQALPVTRLPS
FRFLLSYSIIKEYGASPVLSQGVDPRSTFYVLNPHNNLSSTEEQFRANFSSEAGWRGVVG
EVPRPEQVQEALTKHDLYIYAGHGAGARFLDGQAVLRLSCRAVALLFGCSSAALAVHGNL
EGAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWLGAGPGAPLLYYVNQARQAP
RLKYLIGAAPIAYGLPVSLRSSLAEENLYFQSWSHPQFEKGGGSGGGSGGGSWSHPQFEK
Sequence of entity 2 (B), FASTA
>7NJ1_2 Securin (chains B)
MATLIYVDKENGEPGTRVVAKDGLKLGSGPSIKALDGRSQVSTPRFGKTFDAPPALPKAT
RKALGTVNRATEKSVKTKGPLKQKQPSFSAKKMTEKTVKAKSSVPASDDAYPEIEKFFPF
NPLDFESFDLPEEHQIAHLPLSGVPLMILDEERELEKLFQLGPPSPVKMPSPPWESNLLQ
SPSSILSTLDVELPPVCCDIDI

Primary citation

Structural basis of human separase regulation by securin and CDK1-cyclin B1. Yu, J., Raia, P., Ghent, C.M. et al. Nature (2021) 596:138-142. DOI 10.1038/s41586-021-03764-0 · PubMed

Other PDB entries of the same protein (UniProt Q14674 (AlphaFold model), which also has an AlphaFold model), best resolution first:

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