7O3H: Cytochrome b-c1 complex subunit 1, mitochondrial
Murine CIII2 focus-refined from supercomplex CICIII2. Determined by electron microscopy at 2.6 Å resolution. Released 13 Oct 2021.
- Method
- Electron microscopy
- Resolution
- 2.6 Å
- Organism
- Mus musculus
- Chains
- 21
- Atoms
- 33,700
- Mol. weight
- 496.42 kDa
- Ligands
- PC1, FES, HEC, HEM
- Released
- 13 Oct 2021
Explore 7O3H in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
7O3H contains 210 α-helices and 118 β-strands across 21 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain A: 22 helices, 14 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 4-9 | 6 | |
| α-helix | 12-13 | 2 | |
| β-strand | 15-18 | 4 | 1 |
| β-strand | 24-29 | 6 | 1 |
| β-strand | 34-41 | 8 | 1 |
| α-helix | 45-47 | 3 | |
| α-helix | 55-62 | 8 | |
| β-strand | 67 | 1 | 2 |
| α-helix | 73-81 | 9 | |
| β-strand | 85-90 | 6 | 1 |
| β-strand | 95-102 | 8 | 1 |
| α-helix | 106-118 | 13 | |
| β-strand | 120 | 1 | 2 |
| α-helix | 124-141 | 18 | |
| α-helix | 145-157 | 13 | |
| α-helix | 171-176 | 6 | |
| α-helix | 179-189 | 11 | |
| α-helix | 192-194 | 3 | |
| β-strand | 195-201 | 7 | 1 |
| α-helix | 205-215 | 11 | |
| β-strand | 239-244 | 6 | 3 |
| β-strand | 251-258 | 8 | 3 |
| α-helix | 266-277 | 12 | |
| β-strand | 280-281 | 2 | 3 |
| α-helix | 287-289 | 3 | |
| α-helix | 293-301 | 9 | |
| β-strand | 306-314 | 9 | 3 |
| β-strand | 317-326 | 10 | 3 |
| α-helix | 328-330 | 3 | |
| α-helix | 331-347 | 17 | |
| α-helix | 351-368 | 18 | |
| α-helix | 372-385 | 14 | |
| α-helix | 392-400 | 9 | |
| α-helix | 404-414 | 11 | |
| β-strand | 421-426 | 6 | 3 |
| α-helix | 434-440 | 7 | |
Chain B: 18 helices, 17 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 25-28 | 4 | 4 |
| β-strand | 34-38 | 5 | 4 |
| β-strand | 44-51 | 8 | 4 |
| α-helix | 55-57 | 3 | |
| α-helix | 65-71 | 7 | |
| β-strand | 77 | 1 | 5 |
| β-strand | 80 | 1 | 5 |
| α-helix | 82-91 | 10 | |
| β-strand | 95-100 | 6 | 4 |
| β-strand | 105-112 | 8 | 4 |
| α-helix | 116-128 | 13 | |
| β-strand | 130 | 1 | 5 |
| α-helix | 134-151 | 18 | |
| α-helix | 155-167 | 13 | |
| β-strand | 168 | 1 | 6 |
| α-helix | 188-198 | 11 | |
| α-helix | 201-203 | 3 | |
| β-strand | 204-209 | 6 | 4 |
| α-helix | 213-223 | 11 | |
| α-helix | 232-237 | 6 | |
| β-strand | 239 | 1 | 6 |
| β-strand | 242-247 | 6 | 7 |
| β-strand | 252-261 | 10 | 7 |
| α-helix | 267-279 | 13 | |
| β-strand | 285 | 1 | 1 |
| α-helix | 294-300 | 7 | |
| β-strand | 308-316 | 9 | 7 |
| β-strand | 319-329 | 11 | 7 |
| α-helix | 333-348 | 16 | |
| α-helix | 354-372 | 19 | |
| α-helix | 375-388 | 14 | |
| α-helix | 392-394 | 3 | |
| α-helix | 395-403 | 9 | |
| α-helix | 407-419 | 13 | |
| β-strand | 422-428 | 7 | 7 |
Chain C: 21 helices, 4 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 11-18 | 8 | |
| β-strand | 22-24 | 3 | 8 |
| α-helix | 29-32 | 4 | |
| α-helix | 33-52 | 20 | |
| α-helix | 62-71 | 10 | |
| α-helix | 76-103 | 28 | |
| α-helix | 106-108 | 3 | |
| α-helix | 110-132 | 23 | |
| β-strand | 136 | 1 | 9 |
| α-helix | 137-150 | 14 | |
| α-helix | 157-165 | 9 | |
| α-helix | 172-201 | 30 | |
| α-helix | 213-215 | 3 | |
| β-strand | 217-219 | 3 | 8 |
| α-helix | 223-243 | 21 | |
| α-helix | 253-256 | 4 | |
| β-strand | 258 | 1 | 9 |
| α-helix | 263-265 | 3 | |
| α-helix | 272-281 | 10 | |
| α-helix | 287-303 | 17 | |
| α-helix | 304-306 | 3 | |
| α-helix | 319-339 | 21 | |
| α-helix | 347-360 | 14 | |
| α-helix | 361-365 | 5 | |
| α-helix | 366-376 | 11 | |
Chain D: 11 helices, 9 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 6-10 | 5 | |
| α-helix | 23-35 | 13 | |
| α-helix | 37-39 | 3 | |
| β-strand | 47 | 1 | 10 |
| α-helix | 48-51 | 4 | |
| β-strand | 52 | 1 | 11 |
| β-strand | 56 | 1 | 11 |
| α-helix | 58-66 | 9 | |
| β-strand | 69-72 | 4 | 12 |
| β-strand | 81-84 | 4 | 12 |
| β-strand | 90 | 1 | 10 |
| α-helix | 91-93 | 3 | |
| α-helix | 98-104 | 7 | |
| α-helix | 110-112 | 3 | |
| α-helix | 124-131 | 8 | |
| β-strand | 148-149 | 2 | 13 |
| β-strand | 157-158 | 2 | 13 |
| α-helix | 179-194 | 16 | |
| α-helix | 198-228 | 31 | |
| β-strand | 234-237 | 4 | 3 |
Chain E: 7 helices, 10 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 2-4 | 3 | |
| β-strand | 14 | 1 | 14 |
| α-helix | 16-18 | 3 | |
| α-helix | 29-61 | 33 | |
| α-helix | 66-72 | 7 | |
| α-helix | 74-76 | 3 | |
| β-strand | 86-91 | 6 | 15 |
| β-strand | 94-100 | 7 | 15 |
| α-helix | 103-110 | 8 | |
| β-strand | 132-136 | 5 | 15 |
| α-helix | 146 | 1 | |
| β-strand | 147-148 | 2 | 16 |
| β-strand | 156-157 | 2 | 16 |
| β-strand | 164-165 | 2 | 16 |
| β-strand | 171-173 | 3 | 16 |
| β-strand | 185-187 | 3 | 17 |
| β-strand | 193-195 | 3 | 17 |
Chain F: 7 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 11-24 | 14 | |
| α-helix | 34-36 | 3 | |
| α-helix | 41-48 | 8 | |
| α-helix | 52-70 | 19 | |
| α-helix | 77-79 | 3 | |
| α-helix | 81-82 | 2 | |
| α-helix | 91-109 | 19 | |
Chain G: 2 helices, 2 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 13-18 | 6 | 3 |
| β-strand | 23 | 1 | 14 |
| α-helix | 29-69 | 41 | |
| α-helix | 72-73 | 2 | |
Chain H: 4 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 14-23 | 10 | |
| α-helix | 26-43 | 18 | |
| α-helix | 54-70 | 17 | |
| α-helix | 72-74 | 3 | |
13 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Cytochrome b-c1 complex subunit 1, mitochondrial | A, L | protein | 446 | Mus musculus | Q9CZ13 (AlphaFold model) |
| Cytochrome b-c1 complex subunit 2, mitochondrial | B, M | protein | 439 | Mus musculus | Q9DB77 (AlphaFold model) |
| Cytochrome b | C, N | protein | 381 | Mus musculus | P00158 (AlphaFold model) |
| Cytochrome c1, heme protein, mitochondrial | D, O | protein | 241 | Mus musculus | Q9D0M3 (AlphaFold model) |
| Cytochrome b-c1 complex subunit Rieske, mitochondrial | E, P | protein | 196 | Mus musculus | Q9CR68 |
| Cytochrome b-c1 complex subunit 7 | F, Q | protein | 110 | Mus musculus | Q9D855 |
| Cytochrome b-c1 complex subunit 8 | G, R | protein | 81 | Mus musculus | Q9CQ69 |
| Cytochrome b-c1 complex subunit 6, mitochondrial | H, S | protein | 76 | Mus musculus | P99028 |
| Cytochrome b-c1 complex subunit 9 | J, U | protein | 63 | Mus musculus | Q8R1I1 |
| Cytochrome b-c1 complex subunit 10 | K, V | protein | 56 | Mus musculus | Q9CPX8 |
| Cytochrome b-c1 complex subunit 9 | T | protein | 78 | Mus musculus | Q9CR68 |
Sequence of entity 1 (A, L), FASTA
>7O3H_1 Cytochrome b-c1 complex subunit 1, mitochondrial (chains A, L)
TATFAQALQSVPETQVSILDNGLRVASEQSSHATCTVGVWIDAGSRYETEKNNGAGYFLE
HLAFKGTKNRPGNALEKEVESIGAHLNAYSTREHTAYLIKALSKDLPKVVELLADIVQNS
SLEDSQIEKERDVILREMQENDASMQNVVFDYLHATAFQGTPLAQAVEGPSENVRRLSRT
DLTDYLNRHYKAPRMVLAAAGGVEHQQLLDLAQKHLSSVSRVYEEDAVPGLTPCRFTGSE
IRHRDDALPLAHVAIAVEGPGWANPDNVTLQVANAIIGHYDCTYGGGVHLSSPLASVAVA
NKLCQSFQTFNISYSDTGLLGAHFVCDAMSIDDMVFFLQGQWMRLCTSATESEVTRGKNI
LRNALVSHLDGTTPVCEDIGRSLLTYGRRIPLAEWESRIQEVDAQMLRDICSKYFYDQCP
AVAGYGPIEQLPDYNRIRSGMFWLRF
Sequence of entity 2 (B, M), FASTA
>7O3H_2 Cytochrome b-c1 complex subunit 2, mitochondrial (chains B, M)
SLKVAPKVKTSAAPGGVPLQPQDLEFTKLPNGLVIASLENYAPLSRIGLFVKAGSRYEDS
NNLGTSHLLRLASSLTTKGASSFKITRGIEAVGGKLSVTATRENMAYTVEGIRSDIEILM
EFLLNVTTAPEFRRWEVAALRSQLKIDKAVAFQNSQTRIIENLHDVAYKNALANPLYCPD
YRMGKITSEELHYFVQNHFTSARMALVGLGVSHSVLKQVAEQFLNMRGGLGLAGAKAKYR
GGEIREQNGDNLVHAAIVAESAAIGNAEANAFSVLQHLLGAGPHIKRGNNTTSLLSQSVA
KGSHQPFDVSAFNASYSDSGLFGIYTISQAAAAGEVINAAYNQVKAVAQGNLSSADVQAA
KNKLKAGYLMSVETSEGFLSEIGSQALAAGSYMPPSTVLQQIDSVADADVVKAAKKFVSG
KKSMAASGNLGHTPFLDEL
Sequence of entity 3 (C, N), FASTA
>7O3H_3 Cytochrome b (chains C, N)
MTNMRKTHPLFKIINHSFIDLPAPSNISSWWNFGSLLGVCLMVQIITGLFLAMHYTSDTM
TAFSSVTHICRDVNYGWLIRYMHANGASMFFICLFLHVGRGLYYGSYTFMETWNIGVLLL
FAVMATAFMGYVLPWGQMSFWGATVITNLLSAIPYIGTTLVEWIWGGFSVDKATLTRFFA
FHFILPFIIAALAIVHLLFLHETGSNNPTGLNSDADKIPFHPYYTIKDILGILIMFLILM
TLVLFFPDMLGDPDNYMPANPLNTPPHIKPEWYFLFAYAILRSIPNKLGGVLALILSILI
LALMPFLHTSKQRSLMFRPITQILYWILVANLLILTWIGGQPVEHPFIIIGQLASISYFS
IILILMPISGIIEDKMLKLYP
Sequence of entity 4 (D, O), FASTA
>7O3H_4 Cytochrome c1, heme protein, mitochondrial (chains D, O)
SDLELHPPSYPWSHRGLLSSLDHTSIRRGFQVYKQVCSSCHSMDYVAYRHLVGVCYTEEE
AKALAEEVEVQDGPNDDGEMFMRPGKLSDYFPKPYPNPEAARAANNGALPPDLSYIVRAR
HGGEDYVFSLLTGYCEPPTGVSLREGLYFNPYFPGQAIGMAPPIYTEVLEYDDGTPATMS
QVAKDVATFLRWASEPEHDHRKRMGLKMLLMMGLLLPLTYAMKRHKWSVLKSRKLAYRPP
K
Sequence of entity 5 (E, P), FASTA
>7O3H_5 Cytochrome b-c1 complex subunit Rieske, mitochondrial (chains E, P)
SHTDVKVPDFSDYRRAEVLDSTKSSKESSEARKGFSYLVTATTTVGVAYAAKNVVSQFVS
SMSASADVLAMSKIEIKLSDIPEGKNMAFKWRGKPLFVRHRTKKEIDQEAAVEVSQLRDP
QHDLDRVKKPEWVILIGVCTHLGCVPIANAGDFGGYYCPCHGSHYDASGRIRKGPAPLNL
EVPAYEFTSDDVVVVG
Sequence of entity 6 (F, Q), FASTA
>7O3H_6 Cytochrome b-c1 complex subunit 7 (chains F, Q)
AGRSAVSASSKWLDGFRKWYYNAAGFNKLGLMRDDTLHETEDVKEAIRRLPEDLYNDRMF
RIKRALDLTMRHQILPKDQWTKYEEDKFYLEPYLKEVIRERKEREEWAKK
Sequence of entity 7 (G, R), FASTA
>7O3H_7 Cytochrome b-c1 complex subunit 8 (chains G, R)
GREFGNLARIRHVISYSLSPFEQRAFPSYFSKGIPNVLRRTRERILRVAPPFVVVYLIYT
WGNQEFEQSKRKNPAMYENDK
Sequence of entity 8 (H, S), FASTA
>7O3H_8 Cytochrome b-c1 complex subunit 6, mitochondrial (chains H, S)
GDPKEEEEEELVDPLTTVREHCEQLEKCVKARERLELCDNRVSSRSQTEEDCTEELFDFL
HARDHCVAHKLFKNLK
Sequence of entity 9 (J, U), FASTA
>7O3H_9 Cytochrome b-c1 complex subunit 9 (chains J, U)
SSPTIPSRLYSLLFRRTSTFALTIAVGALFFERAFDQGADAIYEHINEGKLWKHIKHKYE
NKE
Sequence of entity 10 (K, V), FASTA
>7O3H_10 Cytochrome b-c1 complex subunit 10 (chains K, V)
MLSRFLGPRYRELARNWIPTAGMWGTVGAVGLVWATDWRLILDWVPYINGKFKKDD
Sequence of entity 11 (T), FASTA
>7O3H_11 Cytochrome b-c1 complex subunit 9 (chains T)
MLSVAARSGPFAPVLSATSRGVAGALRPLLQGAVPAASEPPVLDVKRPFLCRESLSGQAA
ARPLVATVGLNVPASVRF
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| PC1 | 1,2-diacyl-sn-glycero-3-phosphocholine | C44 H88 N O8 P | 2 |
| FES | FE2/S2 (inorganic) cluster | Fe2 S2 | 2 |
| HEC | Heme C | C34 H36 Fe N4 O4 | 2 |
| HEM | Protoporphyrin IX containing FE | C34 H32 Fe N4 O4 | 4 |
| CDL | Cardiolipin | C81 H156 O17 P2 | 6 |
| 3PE | 1,2-Distearoyl-sn-glycerophosphoethanolamine | C41 H82 N O8 P | 8 |
Primary citation
Structure and assembly of the mammalian mitochondrial supercomplex CIII 2 CIV. Vercellino, I., Sazanov, L.A. Nature (2021) 598:364-367. DOI 10.1038/s41586-021-03927-z · PubMed
Other PDB entries of the same protein (UniProt Q9CZ13 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 7O37 3.2 Å, Murine supercomplex CIII2CIV in the assembled locked conformation
- 7O3C 3.3 Å, Murine supercomplex CIII2CIV in the mature unlocked conformation
- 8PW6 3.3 Å, C respirasome from murine liver
- 8IAR 3.4 Å, Respiratory complex CIII2, focus-refined of type I, Wild type mouse under thermoneutral…
- 8IB7 3.4 Å, Respiratory complex CIII2, focus-refined of type IA, Wild type mouse under cold…
- 8PW7 3.5 Å, A respirasome from murine liver
- 7O3E 3.6 Å, Murine supercomplex CIII2CIV in the intermediate locked conformation
- 8IBC 3.6 Å, Respiratory complex CIII2, focus-refined of type IB, Wild type mouse under cold…
- 8PW5 3.6 Å, CS respirasome from murine liver
- 8UCA 3.7 Å, Formation of I2+III2 supercomplex rescues respiratory chain defects
- 8IBG 3.8 Å, Respiratory complex CIII2, focus-refined of type II, Wild type mouse under cold…
- 8IC5 4.1 Å, Respiratory complex CIII2, focus-refined of type I, PERK -/- mouse under cold temperature
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