7O9F: Bacillus subtilis Ffh

Bacillus subtilis Ffh in complex with ppGpp. Determined by X-ray diffraction at 2.51 Å resolution. Released 2 Feb 2022.

Method
X-ray diffraction
Resolution
2.51 Å
Organism
Bacillus subtilis (strain 168)
Chains
3
Atoms
6,928
Mol. weight
104.37 kDa
Ligands
G4P, MG
Released
2 Feb 2022

Explore 7O9F in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7O9F contains 48 α-helices and 24 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 17 helices, 8 β-strands

ElementResiduesLengthSheet
α-helix4-1916
α-helix25-4117
α-helix46-6015
α-helix63-664
α-helix71-8717
α-helix93-953
α-helix1011
β-strand102-10761
α-helix114-12916
β-strand133-13861
α-helix145-15612
β-strand160-16121
α-helix169-18315
β-strand187-19261
α-helix200-21314
β-strand217-22371
α-helix224-2263
α-helix228-24013
β-strand245-24951
α-helix251-2533
α-helix258-26710
α-helix269-2702
β-strand271-27551
β-strand283-28531
α-helix288-2958
Chain B: 16 helices, 8 β-strands
ElementResiduesLengthSheet
α-helix4-1916
α-helix25-4117
α-helix46-6621
α-helix71-8616
α-helix93-953
α-helix1011
β-strand102-10762
α-helix114-12916
β-strand133-13752
α-helix145-15612
β-strand160-16122
α-helix169-18214
β-strand187-19152
α-helix200-21314
β-strand217-22372
α-helix224-2263
α-helix228-24013
β-strand245-24952
α-helix251-2533
α-helix258-26710
α-helix269-2702
β-strand271-27552
β-strand283-28532
α-helix288-2969
Chain C: 15 helices, 8 β-strands
ElementResiduesLengthSheet
α-helix4-1815
α-helix25-4117
α-helix46-6015
α-helix71-8616
α-helix91-933
β-strand102-10763
α-helix114-12714
β-strand134-13633
α-helix145-15511
β-strand160-16123
α-helix169-18113
β-strand187-19153
α-helix200-2078
β-strand217-22373
α-helix224-2263
α-helix228-24013
β-strand245-24953
α-helix251-2533
α-helix258-26710
α-helix269-2702
β-strand271-27553
β-strand283-28533
α-helix288-2969

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Signal recognition particle proteinA, B, Cprotein310Bacillus subtilis (strain 168)P37105 (AlphaFold model)
Sequence of entity 1 (A, B, C), FASTA
>7O9F_1 Signal recognition particle protein (chains A, B, C)
MGHHHHHHMGFEGLADRLQQTISKIRGKGKVSEQDVKEMMREVRLALLEADVNFKVVKDF
VKKVSERAVGQDVMKSLTPGQQVIKVVQEELTELMGGEESKIAVAKRPPTVIMMVGLQGA
GKTTTSGKLANLLRKKHNRKPMLVAADIYRPAAIKQLETLGKQLDMPVFSLGDQVSPVEI
AKQAIEKAKEEHYDYVILDTAGRLHIDHELMDELTNVKEIANPEEIFLVVDSMTGQDAVN
VAKSFNEQLGLTGVVLTKLDGDTRGGAALSIRAVTNTPIKFAGLGEKLDALEPFHPERMA
SRILGMGDLE

Ligands and cofactors

IDNameFormulaCopies
G4PGuanosine-5',3'-tetraphosphateC10 H17 N5 O17 P43
MGMagnesium ionMg3

Primary citation

Inhibition of SRP-dependent protein secretion by the bacterial alarmone (p)ppGpp. Czech, L., Mais, C.N., Kratzat, H. et al. Nat Commun (2022) 13:1069-1069. DOI 10.1038/s41467-022-28675-0 · PubMed

Other PDB entries of the same protein (UniProt P37105 (AlphaFold model), which also has an AlphaFold model), best resolution first:

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