7OLE: TELO2-TTI1-TTI2-RUVBL1-RUVBL2 complex

Name: PDB 7OLE: Cryo-EM structure of the TELO2-TTI1-TTI2-RUVBL1-RUVBL2 complex
Published: 2021-07-07
License: https://creativecommons.org/publicdomain/zero/1.0/
Keywords: chaperone

Cryo-EM structure of the TELO2-TTI1-TTI2-RUVBL1-RUVBL2 complex. Determined by electron microscopy at 3.41 Å resolution. Released 7 Jul 2021.

Method: Electron microscopy
Resolution: 3.41 Å
Organism: Homo sapiens
Chains: 9
Atoms: 26,995
Mol. weight: 632.6 kDa
Ligands: ADP
Released: 7 Jul 2021

Explore 7OLE in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7OLE contains 193 α-helices and 125 β-strands across 9 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 18 helices, 14 β-strands

Element	Residues	Length	Sheet
β-strand	108	1	1
α-helix	141-143	3
α-helix	146-149	4
β-strand	165	1	2
α-helix	183-186	4
α-helix	207-213	7
β-strand	222-229	8	3
β-strand	257-259	3	4
β-strand	266-268	3	4
α-helix	276-279	4
β-strand	327-334	8	3
α-helix	338-344	7
α-helix	372-386	15
β-strand	395-396	2	5
α-helix	407-412	6
α-helix	413-416	4
β-strand	423-424	2	5
β-strand	427	1	1
β-strand	434-435	2	6
β-strand	443-444	2	6
α-helix	445-447	3
β-strand	452	1	2
α-helix	475-478	4
β-strand	483-484	2	7
α-helix	486-498	13
α-helix	501-504	4
α-helix	505-507	3
α-helix	508-517	10
β-strand	522-523	2	7
α-helix	524	1
α-helix	525-531	7
α-helix	538-549	12

Chain B: 19 helices, 23 β-strands

Element	Residues	Length	Sheet
α-helix	24-26	3
β-strand	33	1	8
β-strand	39	1	8
β-strand	42-43	2	9
β-strand	46-47	2	9
α-helix	50-64	15
β-strand	72-76	5	10
α-helix	83-94	12
β-strand	100-102	3	10
α-helix	105-108	4
α-helix	115-126	12
β-strand	128-129	2	11
β-strand	132-138	7	12
β-strand	141	1	13
β-strand	143	1	14
β-strand	161-163	3	15
β-strand	164	1	14
β-strand	170-172	3	15
α-helix	176-181	6
β-strand	192	1	13
α-helix	224-226	3
β-strand	233-239	7	12
β-strand	242-243	2	11
α-helix	244-250	7
α-helix	270-283	14
β-strand	291-292	2	11
β-strand	295-297	3	10
α-helix	301-303	3
α-helix	306-317	12
β-strand	323-325	3	10
β-strand	328	1	10
β-strand	332-334	3	16
α-helix	335	1
β-strand	341-343	3	16
α-helix	348-351	4
β-strand	354-358	5	10
α-helix	361-363	3
α-helix	364-371	8
α-helix	372-375	4
α-helix	385-396	12
α-helix	412-415	4
α-helix	436-451	16

Chain C: 19 helices, 22 β-strands

Element	Residues	Length	Sheet
α-helix	43-55	13
α-helix	77-86	10
β-strand	93-97	5	17
α-helix	98-101	4
α-helix	108-118	11
β-strand	121-124	4	18
β-strand	127-130	4	19
β-strand	131-133	3	20
β-strand	140	1	21
β-strand	157-162	6	21
β-strand	167-172	6	21
α-helix	180-182	3
β-strand	190-193	4	20
β-strand	194	1	22
β-strand	199	1	22
β-strand	202-204	3	20
β-strand	230-233	4	19
β-strand	236-239	4	18
α-helix	240-248	9
α-helix	273-289	17
β-strand	292-295	4	18
β-strand	298-302	5	17
α-helix	304-306	3
α-helix	309-314	6
α-helix	317-320	4
α-helix	324-325	2
β-strand	326-330	5	17
β-strand	335-337	3	23
α-helix	338	1
β-strand	345-347	3	23
β-strand	360	1	17
α-helix	368-382	15
β-strand	386	1	24
α-helix	388-400	13
α-helix	403-406	4
α-helix	407-409	3
α-helix	410-420	11
β-strand	425	1	24
α-helix	427-433	7
β-strand	437-438	2	10
α-helix	440-450	11

Chain D: 21 helices, 17 β-strands

Element	Residues	Length	Sheet
β-strand	33	1	25
β-strand	39	1	25
α-helix	43-45	3
α-helix	50-64	15
β-strand	72-76	5	26
α-helix	84-94	11
β-strand	100-104	5	26
α-helix	105-108	4
α-helix	115-124	10
β-strand	127-128	2	27
β-strand	137	1	28
β-strand	159	1	29
β-strand	172	1	29
α-helix	178-184	7
β-strand	207	1	28
α-helix	244-250	7
α-helix	270-286	17
β-strand	292-293	2	27
β-strand	295-299	5	26
α-helix	301-303	3
α-helix	306-315	10
β-strand	323-328	6	26
β-strand	332-334	3	30
α-helix	335	1
β-strand	341-343	3	30
α-helix	348-352	5
β-strand	354-357	4	26
α-helix	360-362	3
α-helix	364-378	15
β-strand	382	1	31
α-helix	386-389	4
α-helix	391-394	4
α-helix	399-416	18
β-strand	421	1	31
α-helix	423-426	4
α-helix	436-437	2
α-helix	438-442	5
α-helix	443-450	8

Chain E: 20 helices, 22 β-strands

Element	Residues	Length	Sheet
α-helix	12-14	3
α-helix	17-20	4
β-strand	35-36	2	32
β-strand	39-40	2	32
α-helix	43-57	15
β-strand	65-69	5	33
α-helix	76-87	12
β-strand	93	1	33
β-strand	96-97	2	33
α-helix	98-101	4
α-helix	108-120	13
β-strand	122-130	9	34
β-strand	139	1	35
β-strand	157-159	3	35
β-strand	162-163	2	36
β-strand	166-167	2	36
β-strand	170-172	3	35
α-helix	181-183	3
β-strand	213	1	37
β-strand	215-216	2	37
β-strand	230-237	8	34
α-helix	240-246	7
α-helix	273-288	16
β-strand	292-294	3	34
β-strand	298-302	5	33
α-helix	304-306	3
β-strand	308	1	38
α-helix	309-319	11
α-helix	324-325	2
β-strand	326-331	6	33
β-strand	335-337	3	39
α-helix	338	1
β-strand	339	1	38
β-strand	345-347	3	39
α-helix	352-356	5
β-strand	358-362	5	33
α-helix	368-382	15
α-helix	388-400	13
α-helix	403-406	4
α-helix	410-419	10
α-helix	427-436	10
α-helix	440-450	11

Chain F: 15 helices, 27 β-strands

Element	Residues	Length	Sheet
β-strand	43	1	40
β-strand	46	1	40
α-helix	50-64	15
β-strand	72-76	5	41
α-helix	83-94	12
β-strand	100-104	5	41
α-helix	105-108	4
α-helix	115-125	11
β-strand	127-132	6	42
β-strand	135-136	2	43
β-strand	140	1	44
β-strand	141-142	2	45
β-strand	160-164	5	45
β-strand	170-173	4	45
α-helix	179-184	6
β-strand	192	1	45
β-strand	193-194	2	46
β-strand	212-213	2	46
β-strand	230	1	44
β-strand	235-236	2	43
β-strand	239-243	5	42
α-helix	244-250	7
α-helix	270-286	17
β-strand	290-293	4	42
β-strand	295-299	5	41
α-helix	301-303	3
α-helix	321-322	2
β-strand	323-328	6	41
β-strand	332-334	3	47
β-strand	336	1	48
β-strand	338	1	48
β-strand	341-343	3	47
α-helix	348-351	4
β-strand	354-358	5	41
α-helix	364-377	14
β-strand	382	1	49
α-helix	384-396	13
α-helix	400-415	16
β-strand	421	1	49
α-helix	423-432	10
β-strand	434	1	33
α-helix	436-451	16

Chain H: 37 helices, 0 β-strands

Element	Residues	Length
α-helix	40-54	15
α-helix	64-81	18
α-helix	88-110	23
α-helix	114-134	21
α-helix	140-155	16
α-helix	157-160	4
α-helix	162-175	14
α-helix	180-190	11
α-helix	193-196	4
α-helix	199-218	20
α-helix	222-238	17
α-helix	241-244	4
α-helix	246-255	10
α-helix	264-276	13
α-helix	282-292	11
α-helix	297-308	12
α-helix	314-332	19
α-helix	340-358	19
α-helix	363-375	13
α-helix	380-389	10
α-helix	392-399	8
α-helix	402-413	12
α-helix	417-428	12
α-helix	435-449	15
α-helix	454-471	18
α-helix	472-474	3
α-helix	477-494	18
α-helix	498-515	18
α-helix	521-539	19
α-helix	542-553	12
α-helix	561-571	11
α-helix	583-597	15
α-helix	602-617	16
α-helix	622-635	14
α-helix	638-646	9
α-helix	651-659	9
α-helix	670-681	12

Chain J: 23 helices, 0 β-strands

Element	Residues	Length
α-helix	45-59	15
α-helix	71-82	12
α-helix	88-100	13
α-helix	102-121	20
α-helix	127-146	20
α-helix	156-170	15
α-helix	175-190	16
α-helix	195-210	16
α-helix	214-230	17
α-helix	234-238	5
α-helix	244-262	19
α-helix	266-279	14
α-helix	288-303	16
α-helix	307-328	22
α-helix	332-348	17
α-helix	353-375	23
α-helix	378-394	17
α-helix	397-419	23
α-helix	425-444	20
α-helix	447-450	4
α-helix	458-474	17
α-helix	480-493	14
α-helix	497-507	11

1 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
RuvB-like 1	A, C, E	protein	456	Homo sapiens	Q9Y265 (AlphaFold model)
RuvB-like 2	B, D, F	protein	463	Homo sapiens	Q9Y230 (AlphaFold model)
TELO2-interacting protein 1 homolog,TELO2-interacting protein 1 homolog,TTI1	H	protein	1733	Homo sapiens	O43156 (AlphaFold model)
TELO2-interacting protein 2,TELO2-interacting protein 2,TTI2	J	protein	964	Homo sapiens	Q6NXR4 (AlphaFold model)
Telomere length regulation protein TEL2 homolog	K	protein	491	Homo sapiens	Q9Y4R8

Sequence of entity 1 (A, C, E), FASTA

>7OLE_1 RuvB-like 1 (chains A, C, E)
MKIEEVKSTTKTQRIASHSHVKGLGLDESGLAKQAASGLVGQENAREACGVIVELIKSKK
MAGRAVLLAGPPGTGKTALALAIAQELGSKVPFCPMVGSEVYSTEIKKTEVLMENFRRAI
GLRIKETKEVYEGEVTELTPCETENPMGGYGKTISHVIIGLKTAKGTKQLKLDPSIFESL
QKERVEAGDVIYIEANSGAVKRQGRCDTYATEFDLEAEEYVPLPKGDVHKKKEIIQDVTL
HDLDVANARPQGGQDILSMMGQLMKPKKTEITDKLRGEINKVVNKYIDQGIAELVPGVLF
VDEVHMLDIECFTYLHRALESSIAPIVIFASNRGNCVIRGTEDITSPHGIPLDLLDRVMI
IRTMLYTPQEMKQIIKIRAQTEGINISEEALNHLGEIGTKTTLRYSVQLLTPANLLAKIN
GKDSIEKEHVEEISELFYDAKSSAKILADQQDKYMK

Sequence of entity 2 (B, D, F), FASTA

>7OLE_2 RuvB-like 2 (chains B, D, F)
MATVTATTKVPEIRDVTRIERIGAHSHIRGLGLDDALEPRQASQGMVGQLAARRAAGVVL
EMIREGKIAGRAVLIAGQPGTGKTAIAMGMAQALGPDTPFTAIAGSEIFSLEMSKTEALT
QAFRRSIGVRIKEETEIIEGEVVEIQIDRPATGTGSKVGKLTLKTTEMETIYDLGTKMIE
SLTKDKVQAGDVITIDKATGKISKLGRSFTRARDYDAMGSQTKFVQCPDGELQKRKEVVH
TVSLHEIDVINSRTQGFLALFSGDTGEIKSEVREQINAKVAEWREEGKAEIIPGVLFIDE
VHMLDIESFSFLNRALESDMAPVLIMATNRGITRIRGTSYQSPHGIPIDLLDRLLIVSTT
PYSEKDTKQILRIRCEEEDVEMSEDAYTVLTRIGLETSLRYAIQLITAASLVCRKRKGTE
VQVDDIKRVYSLFLDESRSTQYMKEYQDAFLFNELKGETMDTS

Sequence of entity 3 (H), FASTA

>7OLE_3 TELO2-interacting protein 1 homolog,TELO2-interacting protein 1 homolog,TTI1 (chains H)
MAVFDTPEEAFGVLRPVCVQLTKTQTVENVEHLQTRLQAVSDSALQELQQYILFPLRFTL
KTPGPKRERLIQSVVECLTFVLSSTCVKEQELLQELFSELSACLYSPSSQKPAAVSEELK
LAVIQGLSTLMHSAYGDIILTFYEPSILPRLGFAVSLLLGLAEQEKSKQIKIAALKCLQV
LLLQCDCQDHPRSLDELEQKQLGDLFASFLPGISTALTRLITGDFKQGHSIVVSSLKIFY
KTVSFIMADEQLKRISKVQAKPAVEHRVAELMVYREADWVKKTGDKLTILIKKIIECVSV
HPHWKVRLELVELVEDLLLKCSQSLVECAGPLLKALVGLVNDESPEIQAQCNKVLRHFAD
QKVVVGNKALADILSESLHSLATSLPRLMNSQDDQGKFSTLSLLLGYLKLLGPKINFVLN
SVAHLQRLSKALIQVLELDVADIKIVEERRWNSDDLNASPKTSATQPWNRIQRRYFRFFT
DERIFMLLRQVCQLLGYYGNLYLLVDHFMELYHQSVVYRKQAAMILNELVTGAAGLEVED
LHEKHIKTNPEELREIVTSILEEYTSQENWYLVTCLETEEMGEELMMEHPGLQAITSGEH
TCQVTSFLAFSKPSPTICSMNSNIWQICIQLEGIGQFAYALGKDFCLLLMSALYPVLEKA
GDQTLLISQVATSTMMDVCRACGYDSLQHLINQNSDYLVNGISLNLRHLALHPHTPKVLE
VMLRNSDANLLPLVADVVQDVLATLDQFYDKRAASFVSVLHALMAALAQWFPDTGNLGHL
QEQSLGEEGSHLNQRPAALEKSTTTAEDIEQFLLNYLKEKDVADGNVSDFDNEEEEQSVP
PKVDENDTRPDVEPPLPLQIQIAMDVMERCIHLLSDKNLQIRLKVLDVLDLCVVVLQSHK
NQLLPLAHQAWPSLVHRLTRDAPLAVLRAFKVLRTLGSKCGDFLRSRFCKDVLPKLAGSL
VTQAPISARAGPVYSHTLAFKLQLAVLQGLGPLCERLDLGEGDLNKVADACLIYLSVKQP
VKLQEAARSVFLHLMKVDPDSTWFLLNELYCPVQFTPPHPSLHPVQLHGASGQQNPYTTN
VLQLLKELQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXGXGXXXXXXXXXXXXXXXXXXXXXXXXXGXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

Sequence of entity 4 (J), FASTA

>7OLE_4 TELO2-interacting protein 2,TELO2-interacting protein 2,TTI2 (chains J)
MELDSALEAPSQEDSNLSEELSHSAFGQAFSKILHCLARPEARRGNVKDAVLKDLGDLIE
ATEFDRLFEGTGARLRGMPETLGQVAKALEKYAAPSKEEEGGGDGHSEAAEKAAQVGLLF
LKLLGKVETAKNSLVGPAWQTGLHHLAGPVYIFAITHSLEQPWTTPRSREVAREVLTSLL
QVTECGSVAGFLHGENEDEKGRLSVILGLLKPDLYKESWKNNPAIKHVFSWTLQQVTRPW
LSQHLERVLPASLVISDDYQTENKILGVHCLHHIVLNVPAADLLQYNRAQVLYHAISNHL
YTPEHHLIQAVLLCLLDLFPILEKTLHWKGDGARPTTHCDEVLRLILTHMEPEHRLLLRR
TYARNLPAFVNRLGILTVRHLKRLERVIIGYLEVYDGPEEEARLKILETLKLLMQHTWPR
VSCRLVVLLKALLKLICDVARDPNLTPESVKSALLQEATDCLILLDRCSQGRVKGLLAKI
PQSCEDRKVVNYIRKVQQVSEGAPYNGTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXGXGXXXXXXXXXXXXXXXXXXXXXXXXXGXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXGXXGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXX

Sequence of entity 5 (K), FASTA

>7OLE_5 Telomere length regulation protein TEL2 homolog (chains K)
MEPAPSEVRLAVREAIHALSSSEDGGHIFCTLESLKRYLGEMEPPALPREXXXXXXXXXX
KEEFASAHFSPVLRCLASRLSPAWLELLPHGRLEXXXXXXXXELWASFFLEGPADQAFLV
LMETIEGAAGPSFRLMKMARLLARFLREGRLAVLMEAQCRQQTQPGFILLRETLLGKVVX
XXXXXXXXXXXXXXXXXALPDHLGNRLQQENLAEFFPQNYFRLLGEEVVRVLQAVVDSLQ
GGLDSSVSFVSQVLGKACVHGRQQEILGVLVPRLAALTQGSYLHQRVCWRLVEQVPDRAM
EAVLTGLVEAALGPEVLSRLLGNLVVKNKKAQFVMTQKLLFLQSRLTTPMLQSLLGHLAM
DSQRRPLLLQVLKELLETWGSSSAIRHTPLPQQRHVSKAVLICLAQLGEPELRDSRDELL
ASMMAGVKCRLDSSLPPVRRLGMIVAEVVSARIHPEGPPLKFQYEEDELSLELLALASPQ
PAGDGASEAGT

Ligands and cofactors

ID	Name	Formula	Copies
ADP	Adenosine-5'-diphosphate	C10 H15 N5 O10 P2	5

Primary citation

Structure of the TELO2-TTI1-TTI2 complex and its function in TOR recruitment to the R2TP chaperone. Pal, M., Munoz-Hernandez, H., Bjorklund, D. et al. Cell Rep (2021) 36:109317-109317. DOI 10.1016/j.celrep.2021.109317 · PubMed

Other PDB entries of the same protein (UniProt Q9Y265 (AlphaFold model), which also has an AlphaFold model), best resolution first:

2C9O 2.2 Å, 3D Structure of the human RuvB-like helicase RuvBL1
8QR1 2.4 Å, Cryo-EM structure of the human Tip60 complex
9EMA 2.4 Å, RUVBL1/2 in complex with ATP and CB-6644 inhibitor
6K0R 2.5 Å, Ruvbl1-Ruvbl2 with truncated domain II in complex with phosphorylated Cordycepin
9C57 2.75 Å, Reconstituted P400 Subcomplex of the human TIP60 complex
2XSZ 3.0 Å, The dodecameric human RuvBL1:RuvBL2 complex with truncated domains II
9CAE 3.07 Å, Cryo-EM structure of the reconstituted RuvBL lobe of the human TIP60 complex (composite…
7ZI4 3.2 Å, Cryo-EM structure of the human INO80 complex bound to a WT nucleosome
8X15 3.2 Å, Structure of nucleosome-bound SRCAP-C in the apo state
8X19 3.2 Å, Structure of nucleosome-bound SRCAP-C in the ADP-BeFx-bound state
8X1C 3.2 Å, Structure of nucleosome-bound SRCAP-C in the ADP-bound state
8XVT 3.2 Å, The core subcomplex of human NuA4/TIP60 complex

Browse structure collections

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