7QXS: Human telomerase-DNA-TPP1-POT1 complex

Cryo-EM structure of human telomerase-DNA-TPP1-POT1 complex (with POT1 side chains). Determined by electron microscopy at 3.9 Å resolution. Released 2 Mar 2022.

Method: Electron microscopy
Resolution: 3.9 Å
Organism: Homo sapiens
Chains: 7
Atoms: 17,820
Mol. weight: 434.92 kDa
Released: 2 Mar 2022

Explore 7QXS in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7QXS contains 73 α-helices and 71 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 47 helices, 42 β-strands

Element	Residues	Length	Sheet
α-helix	8-17	10
β-strand	20-23	4	1
α-helix	24-31	8
α-helix	33	1
α-helix	45-52	8
β-strand	54-58	5	1
α-helix	77-90	14
α-helix	96-98	3
β-strand	101-103	3	2
β-strand	119-121	3	2
α-helix	126-132	7
α-helix	135-144	10
α-helix	146-154	9
β-strand	157-161	5	1
β-strand	167-169	3	1
β-strand	325-327	3	3
α-helix	328-331	4
β-strand	332	1	4
α-helix	346-349	4
α-helix	354-365	12
α-helix	380-383	4
α-helix	384-401	18
α-helix	405-412	8
α-helix	445-452	8
α-helix	458-470	13
α-helix	475-478	4
α-helix	481-496	16
β-strand	502-504	3	3
α-helix	505-508	4
α-helix	518-520	3
α-helix	531-550	20
α-helix	552-560	9
β-strand	561-564	4	4
β-strand	569	1	5
β-strand	571	1	5
β-strand	574-577	4	4
α-helix	578-595	18
β-strand	599-600	2	6
α-helix	601-602	2
α-helix	603-611	9
α-helix	615-616	2
β-strand	617-622	6	6
β-strand	625-626	2	7
β-strand	629-630	2	7
β-strand	633-636	4	6
α-helix	654-671	18
α-helix	673-676	4
α-helix	683-698	16
β-strand	709-712	4	8
β-strand	713	1	9
α-helix	722-733	12
β-strand	738-741	4	10
β-strand	742-749	8	1
β-strand	755-760	6	1
β-strand	763-765	3	10
α-helix	766-768	3
α-helix	773-783	11
β-strand	790-796	7	1
β-strand	800-801	2	10
α-helix	802-814	13
β-strand	816-820	5	6
β-strand	823-826	4	6
α-helix	836-853	18
α-helix	855-858	4
β-strand	862-865	4	8
β-strand	869-873	5	8
α-helix	877-889	13
β-strand	891	1	11
β-strand	896	1	11
β-strand	898	1	9
β-strand	904-905	2	8
β-strand	920-921	2	8
β-strand	928-930	3	12
β-strand	933-935	3	12
β-strand	942-944	3	12
β-strand	953	1	13
α-helix	966-976	11
α-helix	978-981	4
α-helix	984-987	4
α-helix	994-1017	24
α-helix	1030-1050	21
β-strand	1058	1	14
β-strand	1062	1	14
α-helix	1067-1082	16
α-helix	1086-1089	4
α-helix	1093-1106	14
α-helix	1110-1118	9
α-helix	1125-1128	4
β-strand	1131	1	13

Chain L: 5 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	19-22	4
α-helix	28-37	10
β-strand	43-44	2	15
α-helix	47-72	26
β-strand	79	1	16
α-helix	81-89	9
α-helix	93-97	5

Chain M: 4 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	39-49	11
β-strand	55	1	16
α-helix	57-85	29
β-strand	89-90	2	15
α-helix	92-102	11
α-helix	106-123	18

Chain O: 5 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	99-103	5
β-strand	113-123	11	17
β-strand	142-147	6	17
β-strand	152-157	6	17
α-helix	159-164	6
α-helix	170-172	3
β-strand	180-187	8	17
β-strand	190	1	18
β-strand	203	1	18
β-strand	204-215	12	17
β-strand	223	1	17
α-helix	224-226	3
α-helix	228-237	10

Chain P: 12 helices, 17 β-strands

Element	Residues	Length	Sheet
β-strand	11	1	19
β-strand	21-32	12	20
α-helix	33-35	3
β-strand	36-37	2	20
β-strand	43-50	8	20
β-strand	56-63	8	20
α-helix	66-68	3
β-strand	78-89	12	20
β-strand	92-105	14	20
β-strand	117	1	20
α-helix	153-155	3
β-strand	161-173	13	21
β-strand	178-184	7	21
α-helix	192	1
β-strand	193	1	22
α-helix	194	1
β-strand	204-205	2	21
α-helix	207-213	7
α-helix	214-216	3
β-strand	218-223	6	21
α-helix	226-231	6
α-helix	233-234	2
β-strand	238-247	10	21
β-strand	261	1	22
β-strand	262-265	4	21
α-helix	270-272	3
β-strand	274-278	5	21
α-helix	279	1
α-helix	283-298	16

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Telomerase reverse transcriptase	A	protein	1132	Homo sapiens	O14746 (AlphaFold model)
RNA (256-mer)	B	RNA	451	Homo sapiens
Histone H2A	L	protein	130	Homo sapiens	B2R5B3 (AlphaFold model)
Histone H2B	M	protein	166	Homo sapiens	B4DR52 (AlphaFold model)
Telomeric DNA	N	DNA	30	Homo sapiens
Adrenocortical dysplasia homolog (Mouse), isoform CRA_a	O	protein	458	Homo sapiens	Q96AP0 (AlphaFold model)
Protection of telomeres protein 1	P	protein	634	Homo sapiens	Q9NUX5

Sequence of entity 1 (A), FASTA

>7QXS_1 Telomerase reverse transcriptase (chains A)
MPRAPRCRAVRSLLRSHYREVLPLATFVRRLGPQGWRLVQRGDPAAFRALVAQCLVCVPW
DARPPPAAPSFRQVSCLKELVARVLQRLCERGAKNVLAFGFALLDGARGGPPEAFTTSVR
SYLPNTVTDALRGSGAWGLLLRRVGDDVLVHLLARCALFVLVAPSCAYQVCGPPLYQLGA
ATQARPPPHASGPRRRLGCERAWNHSVREAGVPLGLPAPGARRRGGSASRSLPLPKRPRR
GAAPEPERTPVGQGSWAHPGRTRGPSDRGFCVVSPARPAEEATSLEGALSGTRHSHPSVG
RQHHAGPPSTSRPPRPWDTPCPPVYAETKHFLYSSGDKEQLRPSFLLSSLRPSLTGARRL
VETIFLGSRPWMPGTPRRLPRLPQRYWQMRPLFLELLGNHAQCPYGVLLKTHCPLRAAVT
PAAGVCAREKPQGSVAAPEEEDTDPRRLVQLLRQHSSPWQVYGFVRACLRRLVPPGLWGS
RHNERRFLRNTKKFISLGKHAKLSLQELTWKMSVRDCAWLRRSPGVGCVPAAEHRLREEI
LAKFLHWLMSVYVVELLRSFFYVTETTFQKNRLFFYRKSVWSKLQSIGIRQHLKRVQLRE
LSEAEVRQHREARPALLTSRLRFIPKPDGLRPIVNMDYVVGARTFRREKRAERLTSRVKA
LFSVLNYERARRPGLLGASVLGLDDIHRAWRTFVLRVRAQDPPPELYFVKVDVTGAYDTI
PQDRLTEVIASIIKPQNTYCVRRYAVVQKAAHGHVRKAFKSHVSTLTDLQPYMRQFVAHL
QETSPLRDAVVIEQSSSLNEASSGLFDVFLRFMCHHAVRIRGKSYVQCQGIPQGSILSTL
LCSLCYGDMENKLFAGIRRDGLLLRLVDDFLLVTPHLTHAKTFLRTLVRGVPEYGCVVNL
RKTVVNFPVEDEALGGTAFVQMPAHGLFPWCGLLLDTRTLEVQSDYSSYARTSIRASLTF
NRGFKAGRNMRRKLFGVLRLKCHSLFLDLQVNSLQTVCTNIYKILLLQAYRFHACVLQLP
FHQQVWKNPTFFLRVISDTASLCYSILKAKNAGMSLGAKGAAGPLPSEAVQWLCHQAFLL
KLTRHRVTYVPLLGSLRTAQTQLSRKLPGTTLTALEAAANPALPSDFKTILD

Sequence of entity 2 (B), FASTA

>7QXS_2 RNA (256-MER) (chains B)
GGGUUGCGGAGGGUGGGCCUGGGAGGGGUGGUGGCCAUUUUUUGUCUAACCCUAACUGAG
AAGGGCGUAGGCGCCGUGCUUUUGCUCCCCGCGCGCUGUUUUUCUCGCUGACUUUCAGCG
GGCGGAAAAGCCUCGGCCUGCCGCCUUCCACCGUUCAUUCUAGAGCAAACAAAAAAUGUC
AGCUGCUGGCCCGUUCGCCCCUCCCGGGGACCUGCGGCGGGUCGCCUGCCCAGCCCCCGA
ACCCCGCCUGGAGGCCGCGGUCGGCCCGGGGCUUCUCCGGAGGCACCCACUGCCACCGCG
AAGAGUUGGGCUCUGUCAGCCGCGGGUCUCUCGGGGGCGAGGGCGAGGUUCAGGCCUUUC
AGGCCGCAGGAAGAGGAACGGAGCGAGUCCCCGCGCGCGGCGCGAUUCCCUGAGCUGUGG
GACGUGCACCCAGGACUCGGCUCACACAUGC

Sequence of entity 3 (L), FASTA

>7QXS_3 Histone H2A (chains L)
MSGRGKQGGKARAKAKTRSSRAGLQFPVGRVRRLLRKGNYAERVGAGAPVYLAAVLEYLT
AEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGKVTIAQGGVLPNIQAVLLPKK
TESHHKAKGK

Sequence of entity 4 (M), FASTA

>7QXS_4 Histone H2B (chains M)
MPDPAKSAPAPKKGSKKAVTKVQKKDGKKRKRSRKESYSVYVYKVLKQVHPDTGISSKAM
GIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVT
KYTSSNPRNLSPTKPGGSEDRQPPPSQLSAIPPFCLVLRAGIAGQV

Sequence of entity 5 (N), FASTA

>7QXS_5 Telomeric DNA (chains N)
TTAGGGTTAGGGTTAGGGTTAGGGTTAGGG

Sequence of entity 6 (O), FASTA

>7QXS_6 Adrenocortical dysplasia homolog (Mouse), isoform CRA_a (chains O)
MAGSGRLVLRPWIRELILGSETPSSPRAGQLLEVLQDAEAAVAGPSHAPDTSDVGATLLV
SDGTHSVRCLVTREALDTSDWEEKEFGFRGTEGRLLLLQDCGVHVQVAEGGAPAEFYLQV
DRFSLLPTEQPRLRVPGCNQDLDVQKKLYDCLEEHLSESTSSNAGLSLSQLLDEMREDQE
HQGALVCLAESCLTLEGPCTAPPVTHWAASRCKATGEAVYTVPSSMLCISENDQLILSSL
GPCQRTQGPELPPPDPALQDLSLTLIASPPSSPSSSGTPALPGHMSSEESGTSISLLPAL
SLAAPDPGQRSSSQPSPAICSAPATLTPRSPHASRTPSSPLQSCTPSLSPRSHVPSPHQA
LVTRPQKPSLEFKEFVGLPCKNRPPFPRTGATRGAQEPCSVWEPPKRHRDGSAFQYEYEP
PCTSLCARVQAVRLPPQLMAWALHFLMDAQPGSEPTPM

Sequence of entity 7 (P), FASTA

>7QXS_7 Protection of telomeres protein 1 (chains P)
MSLVPATNYIYTPLNQLKGGTIVNVYGVVKFFKPPYLSKGTDYCSVVTIVDQTNVKLTCL
LFSGNYEALPIIYKNGDIVRFHRLKIQVYKKETQGITSSGFASLTFEGTLGAPIIPRTSS
KYFNFTTEDHKMVEALRVWASTHMSPSWTLLKLCDVQPMQYFDLTCQLLGKAEVDGASFL
LKVWDGTRTPFPSWRVLIQDLVLEGDLSHIHRLQNLTIDILVYDNHVHVARSLKVGSFLR
IYSLHTKLQSMNSENQTMLSLEFHLHGGTSYGRGIRVLPESNSDVDQLKKDLESANLTAN
QHSDVICQSEPDDSFPSSGSVSLYEVERCQQLSATILTDHQYLERTPLCAILKQKAPQQY
RIRAKLRSYKPRRLFQSVKLHCPKCHLLQEVPHEGDLDIIFQDGATKTPDVKLQNTSLYD
SKIWTTKNQKGRKVAVHFVKNNGILPLSNECLLLIEGGTLSEICKLSNKFNSVIPVRSGH
EDLELLDLSAPFLIQGTIHHYGCKQCSSLRSIQNLNSLVDKTSWIPSSVAEALGIVPLQY
VFVMTFTLDDGTGVLEAYLMDSDKFFQIPASEVLMDDDLQKSVDMIMDMFCPPGIKIDAY
PWLECFIKSYNVTNGTDNQICYQIFDTTVAEDVI

Primary citation

Structural basis of human telomerase recruitment by TPP1-POT1. Sekne, Z., Ghanim, G.E., van Roon, A.M. et al. Science (2022) 375:1173-1176. DOI 10.1126/science.abn6840 · PubMed

Other PDB entries of the same protein (UniProt O14746 (AlphaFold model), which also has an AlphaFold model), best resolution first:

5MEO 1.77 Å, Human Leukocyte Antigen presenting ILGKFLHRL
5MER 1.88 Å, Human Leukocyte Antigen A02 presenting ILAKFLHEL
5MEQ 2.27 Å, Human Leukocyte Antigen A02 presenting ILAKFLHTL
5UGW 2.31 Å, Structure of the human telomerase thumb domain
4B18 2.52 Å, The crystal structure of human Importin alpha 5 with TERT NLS peptide
9Q16 2.7 Å, Human telomerase catalytic core with shelterin protein TPP1, dGpNHpp and DNA primer…
5MEP 2.71 Å, Human Leukocyte Antigen A02 presenting ILGKFLHWL
4MNQ 2.74 Å, TCR-peptide specificity overrides affinity enhancing TCR-MHC interactions
2BCK 2.8 Å, Crystal Structure of HLA-A*2402 Complexed with a telomerase peptide
9Q12 2.8 Å, Human telomerase catalytic core with shelterin protein TPP1 and DNA primer ending in TTAG
5MEN 2.81 Å, Human Leukocyte Antigen A02 presenting ILAKFLHWL, in complex with cognate T-Cell Receptor
9Q10 2.9 Å, Human telomerase catalytic core with shelterin protein TPP1, dTpNHpp and DNA primer…

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