7RB8: ADP state wild type myosin-15-F-actin complex

cryo-EM structure of the ADP state wild type myosin-15-F-actin complex. Determined by electron microscopy at 3.63 Å resolution. Released 28 Jul 2021.

Method: Electron microscopy
Resolution: 3.63 Å
Organisms: Gallus gallus, Mus musculus
Chains: 4
Atoms: 14,226
Mol. weight: 204.88 kDa
Ligands: ADP, MG
Released: 28 Jul 2021

Explore 7RB8 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7RB8 contains 104 α-helices and 81 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 24 helices, 20 β-strands

Element	Residues	Length	Sheet
α-helix	6-7	2
β-strand	8-12	5	1
β-strand	16-21	6	1
β-strand	29-32	4	1
β-strand	35-38	4	2
β-strand	41-42	2	3
β-strand	53	1	2
α-helix	55-60	6
α-helix	62-64	3
β-strand	65-68	4	2
β-strand	71-72	2	4
β-strand	75-76	2	4
α-helix	79-87	9
α-helix	88-92	5
α-helix	98-100	3
β-strand	103-107	5	1
α-helix	113-125	13
β-strand	131-136	6	1
α-helix	137-144	8
β-strand	149-155	7	5
β-strand	160-166	7	5
β-strand	169-170	2	5
α-helix	172-174	3
β-strand	176-178	3	5
α-helix	182-193	12
α-helix	194-196	3
α-helix	203-216	14
α-helix	223-230	8
β-strand	238-241	4	6
β-strand	247-250	4	6
α-helix	253-256	4
α-helix	258-261	4
α-helix	274-282	9
α-helix	287-295	9
β-strand	297-300	4	5
α-helix	302-304	3
α-helix	309-320	12
α-helix	326-327	2
β-strand	329-330	2	5
α-helix	338-348	11
α-helix	350-355	6
β-strand	357-358	2	1
α-helix	359-365	7
α-helix	367-371	5

Chains B and C: 24 helices, 19 β-strands

Element	Residues	Length	Sheet
α-helix	6-7	2
β-strand	8-12	5	14
β-strand	16-21	6	14
β-strand	29-32	4	14
β-strand	35-38	4	15
β-strand	53	1	15
α-helix	55-60	6
α-helix	62-64	3
β-strand	65-68	4	15
β-strand	71-72	2	16
β-strand	75-76	2	16
α-helix	79-87	9
α-helix	88-92	5
α-helix	98-100	3
β-strand	103-107	5	14
α-helix	113-125	13
β-strand	131-136	6	14
α-helix	137-144	8
β-strand	149-155	7	3
β-strand	160-166	7	3
β-strand	169-170	2	3
α-helix	172-174	3
β-strand	176-178	3	3
α-helix	182-193	12
α-helix	194-196	3
α-helix	203-216	14
α-helix	223-230	8
β-strand	238-241	4	17
β-strand	247-250	4	17
α-helix	253-256	4
α-helix	258-261	4
α-helix	274-282	9
α-helix	287-295	9
β-strand	297-300	4	3
α-helix	302-304	3
α-helix	309-320	12
α-helix	326-327	2
β-strand	329-330	2	3
α-helix	338-348	11
α-helix	350-355	6
β-strand	357-358	2	14
α-helix	359-365	7
α-helix	367-371	5

Chain D: 32 helices, 23 β-strands

Element	Residues	Length	Sheet
α-helix	1211-1213	3
α-helix	1219-1231	13
β-strand	1237-1239	3	7
β-strand	1242-1246	5	7
α-helix	1258-1262	5
α-helix	1265-1267	3
α-helix	1272-1273	2
α-helix	1277-1289	13
β-strand	1293-1299	7	7
α-helix	1305-1317	13
α-helix	1332-1334	3
α-helix	1335-1342	8
β-strand	1343-1346	4	8
β-strand	1349-1353	5	8
β-strand	1358-1364	7	7
β-strand	1367-1374	8	7
α-helix	1381-1384	4
β-strand	1393	1	8
α-helix	1394-1401	8
α-helix	1405-1410	6
α-helix	1420-1423	4
α-helix	1436-1448	13
α-helix	1453-1470	18
β-strand	1474	1	9
β-strand	1478	1	10
β-strand	1483	1	10
β-strand	1487	1	9
α-helix	1490-1498	9
α-helix	1504-1512	9
β-strand	1513-1517	5	11
β-strand	1522-1526	5	11
α-helix	1529-1558	30
β-strand	1566-1572	7	7
β-strand	1582	1	12
α-helix	1584-1615	32
α-helix	1617-1619	3
α-helix	1628-1635	8
α-helix	1641-1650	10
α-helix	1656-1666	11
β-strand	1673-1674	2	12
β-strand	1682-1687	6	12
β-strand	1690-1695	6	12
α-helix	1700-1702	3
β-strand	1704	1	13
α-helix	1709-1716	8
α-helix	1721-1733	13
β-strand	1753	1	13
α-helix	1754-1771	18
β-strand	1773-1774	2	7
β-strand	1777-1780	4	7
α-helix	1793-1803	11
α-helix	1807-1812	6
α-helix	1822-1828	7
α-helix	1847-1853	7
α-helix	1872-1885	14

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Actin, alpha skeletal muscle, intermediate form	A, B, C	protein	373	Gallus gallus	P68139 (AlphaFold model)
Isoform 3 of Unconventional myosin-XV	D	protein	685	Mus musculus	Q9QZZ4 (AlphaFold model)

Sequence of entity 1 (A, B, C), FASTA

>7RB8_1 Actin, alpha skeletal muscle, intermediate form (chains A, B, C)
DETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR
GILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQI
MFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAG
RDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYEL
PDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMSGG
TTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEY
DEAGPSIVHRKCF

Sequence of entity 2 (D), FASTA

>7RB8_2 Isoform 3 of Unconventional myosin-XV (chains D)
EDGVEDMTQLEDLQETTVLANLKTRFERNLIYTYIGSILVSVNPYRMFAIYGPEQVQQYS
GRALGENPPHLFAIANLAFAKMLDAKQNQCVIISGESGSGKTEATKLILRCLAAMNQRRD
VMQQIKILEATPLLEAFGNAKTVRNDNSSRFGKFVEIFLEGGVICGAITSQYLLEKSRIV
FQAKNERNYHIFYELLAGLPAQLRQAFSLQEAETYYYLNQGGNCEIAGKSDADDFRRLLA
AMEVLGFTSEDQDSIFRILASILHLGNVYFEKHETDAQEVASVVSAREIQAVAELLQVSP
EGLQKAITFKVTETIREKIFTPLTVESAVDARDAIAKVLYALLFGWLITRVNALVSPKQD
TLSIAILDIYGFEDLSFNSFEQLCINYANENLQYLFNKIVFQEEQEEYIREQMDWREIAF
ADNQPCINLISLKPYGILRILDDQCCFPQATDHTFLQKCHYHHGANPLYSKPKMPLPEFT
IKHYAGKVTYQVHKFLDKNHDQVRQDVLDLFVHSRTRVVAHLFSSHAAQTAPPRLGKSSS
ITRLYKAHTVAAKFQQSLLDLVEKMERCNPLFVRCLKPNHKKEPGLFEPDVMMAQLRYSG
VLETVRIRKEGFPVRLPFQVFIDRYRCLVALKLNVPADGDMCVSLLSRLCTVTPDMYRVG
ISKLFLKEHLHQLLESMRERVQNRA

Ligands and cofactors

ID	Name	Formula	Copies
ADP	Adenosine-5'-diphosphate	C10 H15 N5 O10 P2	4
MG	Magnesium ion	Mg	4

Primary citation

Structural basis for tunable control of actin dynamics by myosin-15 in mechanosensory stereocilia. Gong, R., Jiang, F., Moreland, Z.G. et al. Sci Adv (2022) 8:eabl4733-eabl4733. DOI 10.1126/sciadv.abl4733 · PubMed

Other PDB entries of the same protein (UniProt P68139 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7W4Z 1.15 Å, Crystal structure of fragmin domain-1 in complex with actin (AMPPNP-form)
7W50 1.15 Å, Crystal structure of fragmin domain-1 in complex with actin (ADP-Pi-form)
7W51 1.2 Å, Crystal structure of fragmin domain-1 in complex with actin (ADP-form)
9L2N 1.7 Å, Crystal structure of Cytochalasin D bound to a filamentous conformation actin
7W52 2.0 Å, Crystal structure of fragmin domain-1 (15-160) in complex with actin
1MDU 2.2 Å, Crystal structure of the chicken actin trimer complexed with human gelsolin segment 1…
8D13 2.43 Å, Helical ADP-F-actin
8D14 2.51 Å, Helical ADP-Pi-F-actin
7R94 2.6 Å, T-Plastin-F-actin complex
8C4E 2.6 Å, F-actin decorated by SipA426-685
9JW0 2.69 Å, Structure of the N-terminal 3 domains (V1-V3) villin bound to actin
7YNE 2.7 Å, Crystal structure of fragmin domain-1 (1-160) in complex with G-form actin

Browse structure collections

About this viewer

MolViewer shows 7RB8 directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.