7RSI: The cryo-EM map of KIF18A

The cryo-EM map of KIF18A bound to KIFBP. Determined by electron microscopy at 4.9 Å resolution. Released 8 Sept 2021.

Method
Electron microscopy
Resolution
4.9 Å
Organism
Homo sapiens
Chains
2
Atoms
5,486
Mol. weight
111.93 kDa
Ligands
MG, ADP
Released
8 Sept 2021

Explore 7RSI in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7RSI contains 37 α-helices and 21 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain B: 20 helices, 0 β-strands

ElementResiduesLengthSheet
α-helix6-2116
α-helix39-5012
α-helix87-10418
α-helix113-12513
α-helix137-15115
α-helix155-17218
α-helix202-22019
α-helix224-24017
α-helix246-26217
α-helix266-27914
α-helix302-3054
α-helix307-32519
α-helix326-3283
α-helix347-36317
α-helix369-38113
α-helix390-41021
α-helix415-43319
α-helix441-45212
α-helix456-4594
α-helix465-48521
Chain C: 17 helices, 21 β-strands
ElementResiduesLengthSheet
β-strand13-1861
α-helix19-213
α-helix23-275
α-helix30-312
β-strand3212
β-strand34-3633
β-strand41-4443
β-strand72-7543
β-strand78-8031
α-helix86-916
α-helix94-1018
β-strand106-11381
α-helix119-1235
β-strand12514
β-strand13014
α-helix132-14615
β-strand154-163101
β-strand166-16941
β-strand17611
β-strand178-18145
β-strand187-19045
β-strand19511
α-helix200-21213
α-helix2151
β-strand21616
β-strand22416
β-strand228-237101
α-helix250-2512
β-strand252-25871
α-helix259-2613
α-helix276-2783
α-helix281-29212
α-helix311-3155
α-helix317-3193
β-strand324-33291
β-strand33512
α-helix336-3383
α-helix339-35214

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
KIF-binding proteinBprotein621Homo sapiensQ96EK5 (AlphaFold model)
Kinesin-like protein KIF18ACprotein353Homo sapiensQ8NI77 (AlphaFold model)
Sequence of entity 1 (B), FASTA
>7RSI_1 KIF-binding protein (chains B)
MANVPWAEVCEKFQAALALSRVELHKNPEKEPYKSKYSARALLEEVKALLGPAPEDEDER
PEAEDGPGAGDHALGLPAEVVEPEGPVAQRAVRLAVIEFHLGVNHIDTEELSAGEEHLVK
CLRLLRRYRLSHDCISLCIQAQNNLGILWSEREEIETAQAYLESSEALYNQYMKEVGSPP
LDPTERFLPEEEKLTEQERSKRFEKVYTHNLYYLAQVYQHLEMFEKAAHYCHSTLKRQLE
HNAYHPIEWAINAATLSQFYINKLCFMEARHCLSAANVIFGQTGKISATEDTPEAEGEVP
ELYHQRKGEIARCWIKYCLTLMQNAQLSMQDNIGELDLDKQSELRALRKKELDEEESIRK
KAVQFGTGELCDAISAVEEKVSYLRPLDFEEARELFLLGQHYVFEAKEFFQIDGYVTDHI
EVVQDHSALFKVLAFFETDMERRCKMHKRRIAMLEPLTVDLNPQYYLLVNRQIQFEIAHA
YYDMMDLKVAIADRLRDPDSHIVKKINNLNKSALKYYQLFLDSLRDPNKVFPEHIGEDVL
RPAMLAKFRVARLYGKIITADPKKELENLATSLEHYKFIVDYCEKHPEAAQEIEVELELS
KEMVSLLPTKMERFRTKMALT
Sequence of entity 2 (C), FASTA
>7RSI_2 Kinesin-like protein KIF18A (chains C)
MSVTEEDLCHHMKVVVRVRPENTKEKAAGFHKVVHVVDKHILVFDPKQEEVSFFHGKKTT
NQNVIKKQNKDLKFVFDAVFDETSTQSEVFEHTTKPILRSFLNGYNCTVLAYGATGAGKT
HTMLGSADEPGVMYLTMLHLYKCMDEIKEEKICSTAVSYLEVYNEQIRDLLVNSGPLAVR
EDTQKGVVVHGLTLHQPKSSEEILHLLDNGNKNRTQHPTDMNATSSRSHAVFQIYLRQQD
KTASINQNVRIAKMSLIDLAGSERASTSGAKGTRFVEGTNINRSLLALGNVINALADSKR
KNQHIPYRNSKLTRLLKDSLGGNCQTIMIAAVSPSSVFYDDTYNTLKYANRAK

Ligands and cofactors

IDNameFormulaCopies
MGMagnesium ionMg1
ADPAdenosine-5'-diphosphateC10 H15 N5 O10 P21

Primary citation

Kinesin-binding protein remodels the kinesin motor to prevent microtubule binding. Solon, A.L., Tan, Z., Schutt, K.L. et al. Sci Adv (2021) 7:eabj9812-eabj9812. DOI 10.1126/sciadv.abj9812 · PubMed

Other PDB entries of the same protein (UniProt Q96EK5 (AlphaFold model), which also has an AlphaFold model), best resolution first:

Browse structure collections

About this viewer

MolViewer shows 7RSI directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.