7RX0: Kinesin-like protein KIF7

Complex of AMPPNP-Kif7 and Gli2 Zinc-Finger domain bound to microtubules. Determined by electron microscopy at 3.89 Å resolution. Released 15 Jun 2022.

Method: Electron microscopy
Resolution: 3.89 Å
Organisms: Homo sapiens, Sus scrofa
Chains: 4
Atoms: 9,673
Mol. weight: 168.98 kDa
Ligands: ANP, GTP, G2P, MG
Released: 15 Jun 2022

Explore 7RX0 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7RX0 contains 64 α-helices and 53 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 23 helices, 17 β-strands

Element	Residues	Length	Sheet
β-strand	3-9	7	6
α-helix	11-27	17
β-strand	35	1	7
β-strand	53-55	3	8
β-strand	60	1	7
β-strand	61-63	3	8
β-strand	65-69	5	6
α-helix	72-80	9
α-helix	82-86	5
β-strand	92-94	3	6
α-helix	103	1
α-helix	104-109	6
α-helix	111-113	3
α-helix	115-128	14
β-strand	132-140	9	6
α-helix	148-160	13
β-strand	165-172	8	6
α-helix	173-174	2
α-helix	183-197	15
β-strand	200-201	2	6
β-strand	204-205	2	6
α-helix	206-212	7
α-helix	213-217	5
α-helix	224-243	20
β-strand	246-248	3	9
α-helix	252-258	7
β-strand	269-273	5	9
α-helix	278-281	4
α-helix	288-294	7
α-helix	298-300	3
β-strand	312-321	10	9
α-helix	325-338	14
β-strand	343	1	9
β-strand	351-356	6	9
α-helix	359-363	5
β-strand	373-381	9	9
α-helix	382-384	3
α-helix	385-401	17
α-helix	405-409	5
α-helix	415-436	22

Chain B: 25 helices, 17 β-strands

Element	Residues	Length	Sheet
β-strand	3-9	7	10
α-helix	10-27	18
β-strand	30	1	11
β-strand	36	1	11
α-helix	42-47	4
α-helix	49-51	3
β-strand	53-56	4	12
β-strand	60-63	4	12
β-strand	65-69	5	10
α-helix	72-80	9
α-helix	89-91	3
β-strand	92-94	3	10
α-helix	103	1
α-helix	104-109	6
α-helix	111-128	18
β-strand	132-140	9	10
α-helix	145-160	16
β-strand	165-171	7	10
α-helix	172-173	2
α-helix	183-197	15
β-strand	201-204	4	10
α-helix	206-211	6
α-helix	212-216	5
α-helix	224-240	17
β-strand	246	1	13
α-helix	252-259	8
β-strand	267-268	2	10
β-strand	269-273	5	13
α-helix	288-296	9
α-helix	298-300	3
α-helix	307-309	3
β-strand	312-320	9	13
α-helix	325-338	14
α-helix	340-342	3
β-strand	343	1	13
β-strand	351-356	6	13
α-helix	359-360	2
β-strand	374-381	8	13
α-helix	382-384	3
α-helix	385-399	15
α-helix	405-411	7
α-helix	415-436	22

Chain C: 12 helices, 15 β-strands

Element	Residues	Length	Sheet
α-helix	14-16	3
β-strand	17	1	1
β-strand	20-22	3	1
α-helix	23-25	3
α-helix	27-30	4
β-strand	38-41	4	2
β-strand	46-49	4	2
β-strand	55-56	2	2
β-strand	60	1	1
α-helix	67-70	4
α-helix	71-75	5
α-helix	76-81	6
β-strand	88-94	7	1
α-helix	100-103	4
α-helix	119-131	13
β-strand	141-149	9	1
β-strand	152-155	4	1
β-strand	167-170	4	3
β-strand	176-179	4	3
β-strand	184-185	2	1
α-helix	189-200	12
β-strand	218-225	8	1
β-strand	246-252	7	1
α-helix	267-287	21
α-helix	305-311	7
β-strand	319-326	8	1
α-helix	333-344	12

Chain G: 4 helices, 4 β-strands

Element	Residues	Length	Sheet
β-strand	2	1	4
β-strand	5	1	5
β-strand	8	1	5
β-strand	11	1	4
α-helix	15-22	8
α-helix	23-27	5
α-helix	51-61	11
α-helix	81-91	11

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Kinesin-like protein KIF7	C	protein	544	Homo sapiens	Q2M1P5 (AlphaFold model)
Zinc finger protein GLI2	G	protein	92	Homo sapiens
Tubulin alpha-1A chain	A	protein	451	Sus scrofa	P02550 (AlphaFold model)
Tubulin beta chain	B	protein	445	Sus scrofa	P02554 (AlphaFold model)

Sequence of entity 1 (C), FASTA

>7RX0_1 Kinesin-like protein KIF7 (chains C)
GMGLEAQRLPGAEEAPVRVALRVRPLLPKELLHGHQSCLQVEPGLGRVTLGRDRHFGFHV
VLAEDAGQEAVYQACVQPLLEAFFEGFNATVFAYGQTGSGKTYTMGEASVASLLEDEQGI
VPRAMAEAFKLIDENDLLDCLVHVSYLEVYKEEFRDLLEVGTASRDIQLREDERGNVVLC
GVKEVDVEGLDEVLSLLEMGNAARHTGATHLNHLSSRSHTVFTVTLEQRGRAPSRLPRPA
PGQLLVSKFHFVDLAGSERVLKTGSTGERLKESIQINSSLLALGNVISALGDPQRRGSHI
PYRDSKITRILKDSLGGNAKTVMIACVSPSSSDFDETLNTLNYASRAQNIRNRATVNWRP
EAERPPEETASGARGPPRHRSETRIIHRGRRAPGPATASAAAAMRLGAECARYRACTDAA
YSLLRELQAEPGLPGAAARKVRDWLCAVEGERSALSSASGPDSGIESASVEDQAAQGAGG
RKEDEGAQQLLTLQNQVARLEEENRDFLAALEDAMEQYKLQSDRLREQQEEMVELRLRLE
LVRP

Sequence of entity 2 (G), FASTA

>7RX0_2 Zinc finger protein GLI2 (chains G)
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

Sequence of entity 3 (A), FASTA

>7RX0_3 Tubulin alpha-1A chain (chains A)
MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGK
HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLD
RIRKLADQCTGLQGFSVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTA
VVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITA
SLRFDGALNVDLTEFQTNLVPYPRAHFPLATYAPVISAEKAYHEQLSVAEITNACFEPAN
QMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYEPP
TVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
AREDMAALEKDYEEVGVDSVEGEGEEEGEEY

Sequence of entity 4 (B), FASTA

>7RX0_4 Tubulin beta chain (chains B)
MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEAAGNKYV
PRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV
RKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVV
EPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCL
RFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMM
AACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRG
LKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS
EYQQYQDATADEQGEFEEEGEEDEA

Ligands and cofactors

ID	Name	Formula	Copies
ANP	Phosphoaminophosphonic acid-adenylate ester	C10 H17 N6 O12 P3	1
GTP	Guanosine-5'-triphosphate	C10 H16 N5 O14 P3	1
G2P	Phosphomethylphosphonic acid guanylate ester	C11 H18 N5 O13 P3	1
MG	Magnesium ion	Mg	1

Primary citation

Cytoskeletal regulation of a transcription factor by DNA mimicry via coiled-coil interactions. Haque, F., Freniere, C., Ye, Q. et al. Nat Cell Biol (2022) 24:1088-1098. DOI 10.1038/s41556-022-00935-7 · PubMed

Other PDB entries of the same protein (UniProt Q2M1P5 (AlphaFold model), which also has an AlphaFold model), best resolution first:

4A14 1.6 Å, Human KIF7, a kinesin involved in hedgehog signalling
2XT3 1.88 Å, Human KIF7, a kinesin involved in hedgehog signalling
6MLQ 4.2 Å, Cryo-EM structure of microtubule-bound Kif7 in the ADP state
6MLR 4.2 Å, Cryo-EM structure of microtubule-bound Kif7 in the AMPPNP state

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