7T0L: HLA-B*27:05

HLA-B*27:05 in complex with the pan-HLA-Ia monoclonal antibody W6/32. Determined by X-ray diffraction at 3.0 Å resolution. Released 7 Dec 2022.

Method: X-ray diffraction
Resolution: 3.0 Å
Organisms: Homo sapiens, synthetic construct, Mus musculus
Chains: 10
Atoms: 12,916
Mol. weight: 182.94 kDa
Released: 7 Dec 2022

Explore 7T0L in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7T0L contains 47 α-helices and 143 β-strands across 8 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 9 helices, 19 β-strands

Element	Residues	Length	Sheet
β-strand	3-12	10	1
β-strand	21-28	8	1
β-strand	31-37	7	1
β-strand	46-47	2	1
α-helix	57-84	28
β-strand	94-103	10	1
β-strand	109-118	10	1
β-strand	121-126	6	1
β-strand	133-135	3	1
α-helix	140-149	10
α-helix	152-161	10
α-helix	163-174	12
α-helix	176-179	4
β-strand	183	1	2
α-helix	184-185	2
β-strand	186-195	10	3
β-strand	198-208	11	3
β-strand	209	1	2
β-strand	214-219	6	4
β-strand	222-223	2	4
α-helix	225-227	3
β-strand	228-230	3	3
α-helix	231-233	3
β-strand	234-235	2	3
β-strand	241-250	10	3
α-helix	253-255	3
β-strand	257-262	6	4
β-strand	270-272	3	4

Chain B: 1 helix, 12 β-strands

Element	Residues	Length	Sheet
β-strand	3	1	5
β-strand	6	1	6
β-strand	9-11	3	6
β-strand	21-30	10	6
β-strand	31	1	5
β-strand	35-41	7	7
β-strand	44-45	2	7
α-helix	46	1
β-strand	50-51	2	6
β-strand	55-56	2	6
β-strand	62-70	9	6
β-strand	78-84	7	7
β-strand	91-94	4	7

Chain D: 10 helices, 20 β-strands

Element	Residues	Length	Sheet
β-strand	3-12	10	8
β-strand	21-28	8	8
β-strand	31-37	7	8
β-strand	46-47	2	8
α-helix	57-81	25
α-helix	82-86	5
β-strand	94-103	10	8
β-strand	109-118	10	8
β-strand	122-125	4	8
β-strand	126	1	9
β-strand	133	1	9
α-helix	138-149	12
α-helix	153-159	7
α-helix	160-164	5
α-helix	165-174	10
α-helix	176-179	4
β-strand	183	1	10
β-strand	186-195	10	11
β-strand	198-208	11	11
β-strand	209	1	10
β-strand	213-219	7	12
β-strand	223	1	12
α-helix	225-227	3
β-strand	228-230	3	11
α-helix	231-233	3
β-strand	234-235	2	11
β-strand	241-250	10	11
α-helix	253-255	3
β-strand	257-263	7	12
β-strand	270	1	12

Chain E: 1 helix, 11 β-strands

Element	Residues	Length	Sheet
β-strand	3	1	13
β-strand	6-11	6	14
β-strand	21-30	10	14
β-strand	31	1	13
β-strand	35-41	7	15
β-strand	44-45	2	15
α-helix	46	1
β-strand	50-51	2	14
β-strand	55-56	2	14
β-strand	62-70	9	14
β-strand	78-84	7	15
β-strand	91-94	4	15

Chain G: 6 helices, 21 β-strands

Element	Residues	Length	Sheet
β-strand	2-6	5	16
β-strand	10-11	2	17
β-strand	15-24	10	16
β-strand	33-38	6	18
β-strand	45-50	6	18
β-strand	56-58	3	18
α-helix	60-62	3
β-strand	66-71	6	16
α-helix	72-74	3
β-strand	76-84	9	16
α-helix	86-88	3
β-strand	90-96	7	18
β-strand	108-109	2	18
β-strand	113-115	3	18
β-strand	116-117	2	17
α-helix	121-122	2
β-strand	123	1	19
β-strand	126-130	5	20
α-helix	131-133	3
β-strand	141-151	11	20
β-strand	152	1	19
β-strand	157-160	4	21
β-strand	169-176	8	20
β-strand	181-190	10	20
β-strand	200-205	6	21
β-strand	210-215	6	21
α-helix	216	1

Chain H: 5 helices, 21 β-strands

Element	Residues	Length	Sheet
β-strand	2-6	5	22
β-strand	10-11	2	23
β-strand	17-24	8	22
β-strand	33-38	6	24
β-strand	45-50	6	24
β-strand	56-58	3	24
α-helix	60-62	3
β-strand	66-71	6	22
β-strand	76-81	6	22
α-helix	86-88	3
β-strand	90-96	7	24
β-strand	108-109	2	24
β-strand	113-115	3	24
β-strand	116-117	2	23
α-helix	121-122	2
β-strand	123	1	25
β-strand	126-130	5	26
β-strand	141-151	11	26
β-strand	152	1	25
β-strand	157-160	4	27
α-helix	161-163	3
β-strand	169-176	8	26
β-strand	181-190	10	26
β-strand	201-205	5	27
α-helix	206-208	3
β-strand	210-214	5	27

Chain K: 7 helices, 21 β-strands

Element	Residues	Length	Sheet
β-strand	5-8	4	28
β-strand	11-15	5	29
β-strand	20-30	11	28
β-strand	34-39	6	29
α-helix	44-45	2
β-strand	46-50	5	29
β-strand	54-55	2	29
α-helix	56	1
β-strand	63-67	5	28
β-strand	69-76	8	28
α-helix	81-83	3
β-strand	86-91	6	29
β-strand	100	1	29
β-strand	104-109	6	29
β-strand	118-120	3	30
α-helix	121-123	3
α-helix	124-129	6
β-strand	131-133	3	31
β-strand	135-141	7	30
β-strand	147-152	6	32
β-strand	155-156	2	32
β-strand	161-165	5	30
α-helix	166-169	4
β-strand	175-181	7	30
β-strand	182-184	3	31
α-helix	185-190	6
β-strand	194-199	6	32
β-strand	209-211	3	32

Chain L: 8 helices, 18 β-strands

Element	Residues	Length	Sheet
β-strand	5-8	4	33
β-strand	11-15	5	34
β-strand	20-30	11	33
β-strand	34-39	6	34
β-strand	45-50	6	34
β-strand	54-55	2	34
α-helix	56	1
β-strand	63-76	14	33
α-helix	81-83	3
β-strand	86-91	6	34
β-strand	100	1	34
β-strand	104-109	6	34
α-helix	113-115	3
β-strand	116-120	5	35
α-helix	121-123	3
α-helix	126-129	4
β-strand	131-141	11	35
β-strand	147-152	6	36
β-strand	155-157	3	36
β-strand	161-165	5	35
α-helix	166-169	4
β-strand	175-184	10	35
α-helix	185-188	4
β-strand	194-199	6	36
α-helix	206	1
β-strand	207-211	5	36

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
MHC class I antigen	A, D	protein	276	Homo sapiens	A3F718 (AlphaFold model)
Beta-2-microglobulin	B, E	protein	99	Homo sapiens	P61769 (AlphaFold model)
PHE-ARG-TYR-ASN-GLY-LEU-ILE-HIS-ARG peptide	C, F	protein	9	synthetic construct
IgG2a heavy chain	G, H	protein	218	Mus musculus
Light chain kappa	K, L	protein	211	Mus musculus

Sequence of entity 1 (A, D), FASTA

>7T0L_1 MHC class I antigen (chains A, D)
GSHSMRYFHTSVSRPGRGEPRFITVGYVDDTLFVRFDSDAASPREEPRAPWIEQEGPEYW
DRETQISKAKAQTDREDLRTLLRYYNQSEAGSHTLQNMYGCDVGPDGRLLRGYHQNAYDG
KDYIALNEDLSSWTAADTAAQITQRKWEAARVAEQLRAYLEGECVEWLRRYLENGKETLQ
RADPPKTHVTHHPISDHEATLRCWALGFYPAEITLTWQRDGEDQTQDTELVETRPAGDRT
FQKWAAVVVPSGEEQRYTCHVQHEGLPKPLTLRWEP

Sequence of entity 2 (B, E), FASTA

>7T0L_2 Beta-2-microglobulin (chains B, E)
IQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDW
SFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM

Sequence of entity 3 (C, F), FASTA

>7T0L_3 PHE-ARG-TYR-ASN-GLY-LEU-ILE-HIS-ARG peptide (chains C, F)
FRYNGLIHR

Sequence of entity 4 (G, H), FASTA

>7T0L_4 IgG2a heavy chain (chains G, H)
VQLKQSGPGLVQPSQSLSLTCTVSGFSLTSYGVHWVRQPPGKGLEWLGVIWSGGSTDYNA
AFISRLSIRKDNSKSQVFFKMNSLQADDTAIYYCARTFTTSTSAWFAYWGQGTLVTVSAA
KTTAPSVYPLAPVCGDTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSDL
YTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKIEP

Sequence of entity 5 (K, L), FASTA

>7T0L_5 Light chain kappa (chains K, L)
SIVMTQTPKFLLVSAGDRVTITCKASQSVSNDVAWYQQKPGQSPKLLIYYASNRYTGVPD
RFTGSGYGTDFTFTISTVQAEDLAVYFCQQDYSSPPWTFGGGTKLEIRRADAAPTVSIFP
PSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTL
TLTKDEYERHNSYTCEATHKTSTSPIVKSFN

Primary citation

The Structural Basis for Recognition of Human Leukocyte Antigen Class I Molecules by the Pan-HLA Antibody W6/32. Pymm, P., Saunders, P.M., Anand, S. et al. J Immunol (2024) 213:876-885. DOI 10.4049/jimmunol.2400328 · PubMed

Other PDB entries of the same protein (UniProt A3F718 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9PBG 2.0 Å, TCR 19.2 complex with YEIH-HLA B*27:05
9PBH 2.13 Å, AS19.2 TCR complex with PSG5-HLA B*27:05
8CX4 2.2 Å, TCR-antigen complex AS8.4-YEIH-HLA*B27
7N2R 2.28 Å, AS4.3-PRPF3-HLA*B27
7N2O 2.3 Å, AS4.2-yeih-HLA*B27
7N2S 2.37 Å, AS3.1-PRPF3-HLA*B27
7N2P 2.5 Å, AS4.3-RNASEH2b-HLA*B27
7N2N 2.6 Å, TCR-antigen complex AS4.2-PRPF3-HLA*B27
7N2Q 2.7 Å, AS4.3-yeih-HLA*B27

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