7TIT: Cardiac actin

Cardiac thin filament decorated with regulatory M-domain of cardiac myosin binding protein C. Determined by electron microscopy at 8.0 Å resolution. Released 23 Nov 2022.

Method: Electron microscopy
Resolution: 8.0 Å
Organisms: Sus scrofa, Homo sapiens
Chains: 16
Atoms: 22,272
Mol. weight: 445.83 kDa
Released: 23 Nov 2022

Explore 7TIT in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7TIT contains 142 α-helices and 114 β-strands across 16 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chains A, B, C, D, E and F: 20 helices, 19 β-strands

Element	Residues	Length	Sheet
β-strand	10-11	2	1
β-strand	16-19	4	1
β-strand	29-32	4	1
α-helix	54-57	4
α-helix	58-61	4
β-strand	62	1	2
β-strand	65	1	2
β-strand	72	1	3
β-strand	75	1	3
α-helix	80-88	9
α-helix	89-93	5
α-helix	98-100	3
β-strand	103-107	5	1
α-helix	113-127	15
β-strand	131-136	6	1
α-helix	137-145	9
β-strand	149	1	4
β-strand	152-154	3	5
β-strand	161-162	2	5
β-strand	166	1	4
β-strand	176-177	2	5
α-helix	182-195	14
α-helix	203-215	13
α-helix	224-232	9
β-strand	238-241	4	6
β-strand	247-250	4	6
α-helix	253-256	4
α-helix	258-262	5
α-helix	274-284	11
α-helix	289-296	8
β-strand	297-300	4	5
α-helix	302-304	3
α-helix	309-320	12
β-strand	329-330	2	5
α-helix	335-348	14
α-helix	350-355	6
β-strand	357-358	2	1
α-helix	359-365	7
α-helix	368-370	3

Chains G, H, I, J, K and L: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	321-326	6
α-helix	333-339	7
α-helix	345-356	12

Chains M, N, O and P: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	63-195	133

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
cardiac actin	A, B, C, D, E, F	protein	375	Sus scrofa	P68137 (AlphaFold model)
Myosin-binding protein C, cardiac-type	G, H, I, J, K, L	protein	220	Homo sapiens	Q14896 (AlphaFold model)
tropomyosin model	M, N, O, P	protein	135	Sus scrofa

Sequence of entity 1 (A, B, C, D, E, F), FASTA

>7TIT_1 cardiac actin (chains A, B, C, D, E, F)
DDEETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQS
KRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMT
QIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDL
AGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSY
ELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVLS
GGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQ
EYDEAGPSIVHRKCF

Sequence of entity 2 (G, H, I, J, K, L), FASTA

>7TIT_2 Myosin-binding protein C, cardiac-type (chains G, H, I, J, K, L)
DDPIGLFVMRPQDGEVTVGGSITFSARVAGASLLKPPVVKWFKGKWVDLSSKVGQHLQLH
DSYDRASKVYLFELHITDAQPAFTGSYRCEVSTKDKFDCSNFNLTVHEAMGTGDLDLLSA
FRRTSLAGGGRRISDSHEDTGILDFSSLLKKRDSFRTPRDSKLEAPAEEDVWEILRQAPP
SEYERIAFQYGVTDLRGMLKRLKGMRRDEKKSTAFQKKLE

Sequence of entity 3 (M, N, O, P), FASTA

>7TIT_3 tropomyosin model (chains M, N, O, P)
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXX

Primary citation

Cryo-Electron Microscopy Reveals Cardiac Myosin Binding Protein-C M-Domain Interactions with the Thin Filament. Risi, C.M., Villanueva, E., Belknap, B. et al. J Mol Biol (2022) 434:167879-167879. DOI 10.1016/j.jmb.2022.167879 · PubMed

Other PDB entries of the same protein (UniProt P68137 (AlphaFold model), which also has an AlphaFold model), best resolution first:

4CBW 2.5 Å, Crystal structure of Plasmodium berghei actin I with D-loop from muscle actin
8EFH 3.3 Å, Helical reconstruction of the human cardiac actin-tropomyosin-myosin complex in complex…
8DD0 3.5 Å, The structure of the native cardiac thin filament junction region
7KO5 7.8 Å, Structure of cardiac native thin filament at pCa=5.8 having upper and lower troponins in…
7KOR 7.8 Å, Structure of cardiac native thin filament at pCa=5.8 having upper troponin in Ca2+ bound…
5NOL 8.0 Å, Ca2+-induced Movement of Tropomyosin on Native Cardiac Thin Filaments - "Closed" state
7KO4 8.0 Å, Structure of cardiac native thin filament at pCa=5.8 having upper and lower troponins in…
7TIJ 8.0 Å, Cardiac F-actin decorated with regulatory M-domain of cardiac myosin binding protein C
7TJ7 8.0 Å, Cardiac thin filament decorated with C1 Ig-domain and regulatory M-domain of cardiac…
7KON 8.1 Å, Structure of upper Tn Ca2+ free (rotated) and lower Tn Ca2+ bound cardiac native thin…
7KO7 8.3 Å, Structure of the native cardiac thin filament at pCa=5.8 having upper Tn in Ca2+ free…
5NOG 11.0 Å, Ca2+-induced Movement of Tropomyosin on Native Cardiac Thin Filaments - "Blocked" state

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