7TYR: Basal state of the Artemis:DNA-PKcs complex

Cryo-EM structure of the basal state of the Artemis:DNA-PKcs complex (see COMPND 13/14). Determined by electron microscopy at 3.33 Å resolution. Released 20 Jul 2022.

Method
Electron microscopy
Resolution
3.33 Å
Organism
Homo sapiens
Chains
2
Atoms
31,610
Mol. weight
550.17 kDa
Released
20 Jul 2022

Explore 7TYR in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7TYR contains 243 α-helices and 31 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 242 helices, 31 β-strands

ElementResiduesLengthSheet
α-helix4-1815
α-helix32-4312
α-helix51-588
α-helix65-717
α-helix72-743
α-helix82-9514
α-helix101-11515
α-helix124-13512
α-helix149-16214
α-helix168-18013
α-helix193-20816
α-helix217-23317
α-helix246-25611
α-helix267-27711
α-helix279-2813
α-helix287-2893
α-helix290-30112
α-helix307-32923
α-helix336-35015
α-helix356-36813
α-helix371-3766
α-helix380-39516
α-helix410-42213
α-helix432-44413
α-helix446-4483
α-helix451-46919
α-helix473-49119
α-helix494-4974
α-helix520-5223
α-helix524-5274
α-helix528-5358
α-helix538-54710
α-helix555-5584
α-helix560-57819
β-strand583-58421
α-helix597-6004
α-helix602-6043
β-strand613-61421
α-helix617-63317
α-helix637-6393
α-helix643-6453
α-helix646-65914
α-helix664-68017
α-helix689-6913
α-helix698-71720
α-helix718-7203
α-helix723-73412
α-helix738-7436
α-helix749-75911
α-helix763-77917
α-helix782-7854
α-helix786-7883
α-helix789-80113
α-helix802-8043
α-helix848-86215
α-helix864-8674
α-helix868-8714
α-helix877-8793
β-strand88412
β-strand893-89533
β-strand904-90633
α-helix911-9199
α-helix924-94623
α-helix948-9492
α-helix955-9573
α-helix959-97214
α-helix979-99517
α-helix1004-101613
α-helix1024-104320
α-helix1056-106712
α-helix1072-109120
α-helix1093-10964
α-helix1101-111313
α-helix1114-11163
α-helix1123-114018
α-helix1142-11454
β-strand114914
α-helix1158-11592
β-strand116314
α-helix1165-117410
α-helix1175-11773
α-helix1181-119414
α-helix1195-11973
α-helix1204-122522
α-helix1234-12374
α-helix1250-127223
α-helix1278-12825
α-helix1290-12967
α-helix1297-13015
α-helix1306-13094
α-helix1324-135128
α-helix1355-13595
α-helix1360-13623
α-helix1365-137713
α-helix1379-13824
α-helix1389-140719
α-helix1412-142211
α-helix1425-14317
α-helix1442-146120
α-helix1464-14674
α-helix1478-149013
α-helix1500-15023
α-helix1505-152117
α-helix1525-15328
β-strand153715
β-strand1540-154126
β-strand1550-155126
β-strand155415
α-helix1555-15628
α-helix1564-157310
α-helix1575-158410
α-helix1590-160516
α-helix1614-162310
α-helix1626-16294
α-helix1630-16334
α-helix1639-165618
α-helix1668-168013
α-helix1686-16949
α-helix1696-16994
α-helix1705-17084
α-helix1709-172113
α-helix1734-175320
α-helix1756-176611
α-helix1775-178713
α-helix1791-180515
α-helix1813-18186
α-helix1819-18246
α-helix1825-18295
α-helix1833-185119
α-helix1860-188223
α-helix1886-18894
α-helix1912-192312
α-helix1935-195319
α-helix1971-19755
β-strand202317
α-helix2035-204713
β-strand205317
α-helix2094-210512
α-helix21111
α-helix2124-213411
α-helix2140-215112
α-helix2154-21574
α-helix2165-21728
α-helix2184-219411
α-helix2206-221914
α-helix2228-224417
α-helix2253-22608
α-helix2270-228112
α-helix2297-23004
α-helix2301-23055
α-helix2317-233216
α-helix2339-235315
α-helix2357-236812
α-helix2372-23776
α-helix2379-23857
α-helix2392-240211
α-helix2411-24177
α-helix2420-24256
α-helix2431-244212
α-helix2448-245912
α-helix2467-248418
α-helix2493-250816
α-helix2509-25113
α-helix2515-252612
α-helix2537-25448
α-helix2548-25503
α-helix2554-256310
α-helix2566-25683
β-strand257518
α-helix2589-25902
β-strand278618
α-helix2788-279811
α-helix2802-282221
α-helix2826-284520
α-helix2852-286413
α-helix2867-28704
α-helix2873-288311
α-helix2886-289914
α-helix2911-29122
α-helix2916-29172
α-helix2918-293316
α-helix2936-29427
α-helix2943-29475
α-helix2951-296111
α-helix2965-297713
α-helix2985-29873
α-helix2988-300417
α-helix3008-301710
α-helix3027-30315
α-helix3034-30396
α-helix3041-305313
α-helix3060-307011
α-helix3073-30819
α-helix3083-309210
α-helix3096-311520
α-helix3122-314524
α-helix3148-31503
α-helix3155-316612
α-helix3176-319621
α-helix3206-32105
α-helix3213-32208
α-helix3227-324822
α-helix3252-326615
α-helix3270-328819
α-helix3293-330412
α-helix3310-33167
α-helix3320-334223
α-helix3347-33504
α-helix3353-336311
α-helix3372-339322
α-helix3406-342924
α-helix3431-34322
α-helix3437-34415
α-helix3442-345514
α-helix3459-34635
α-helix3465-347410
α-helix3476-348611
α-helix3487-34893
α-helix3492-34943
α-helix3499-35035
α-helix3509-35113
α-helix3512-352413
α-helix3530-35378
α-helix3546-356217
α-helix3567-357610
α-helix3583-359513
α-helix3607-36137
α-helix3614-36185
α-helix3627-364317
α-helix3647-36537
α-helix3657-36604
α-helix3661-367212
β-strand367919
α-helix3680-36834
α-helix3685-36884
β-strand3700110
α-helix37171
β-strand3718110
β-strand3719-3722411
β-strand372519
β-strand3727-3728211
β-strand3736-3742711
β-strand3747-3754811
α-helix3759-377618
α-helix3779-37824
β-strand3793-3797511
β-strand3800-3804511
α-helix3805-38062
β-strand3809-3811312
α-helix3812-38198
α-helix3822-38298
α-helix3835-384612
α-helix3853-38608
α-helix3864-387512
α-helix3882-38909
β-strand389112
α-helix3894-391825
α-helix3925-39273
β-strand3928-3931412
β-strand3937-3939312
α-helix3948-39514
α-helix3965-39706
α-helix3979-399214
α-helix3996-400611
α-helix4013-402210
α-helix4040-405112
β-strand4054113
α-helix4056-406712
α-helix4074-40829
β-strand4096113
α-helix4100-411112
α-helix4114-41174
α-helix4122-41243
Chain C: 1 helix, 0 β-strands
ElementResiduesLengthSheet
α-helix398-4025

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
DNA-dependent protein kinase catalytic subunitAprotein4128Homo sapiensP78527
Protein artemisCprotein707Homo sapiensQ96SD1 (AlphaFold model)
Sequence of entity 1 (A), FASTA
>7TYR_1 DNA-dependent protein kinase catalytic subunit (chains A)
MAGSGAGVRCSLLRLQETLSAADRCGAALAGHQLIRGLGQECVLSSSPAVLALQTSLVFS
RDFGLLVFVRKSLNSIEFRECREEILKFLCIFLEKMGQKIAPYSVEIKNTCTSVYTKDRA
AKCKIPALDLLIKLLQTFRSSRLMDEFKIGELFSKFYGELALKKKIPDTVLEKVYELLGL
LGEVHPSEMINNAENLFRAFLGELKTQMTSAVREPKLPVLAGCLKGLSSLLCNFTKSMEE
DPQTSREIFNFVLKAIRPQIDLKRYAVPSAGLRLFALHASQFSTCLLDNYVSLFEVLLKW
CAHTNVELKKAALSALESFLKQVSNMVAKNAEMHKNKLQYFMEQFYGIIRNVDSNNKELS
IAIRGYGLFAGPCKVINAKDVDFMYVELIQRCKQMFLTQTDTGDDRVYQMPSFLQSVASV
LLYLDTVPEVYTPVLEHLVVMQIDSFPQYSPKMQLVCCRAIVKVFLALAAKGPVLRNCIS
TVVHQGLIRICSKPVVLPKGPESESEDHRASGEVRTGKWKVPTYKDYVDLFRHLLSSDQM
MDSILADEAFFSVNSSSESLNHLLYDEFVKSVLKIVEKLDLTLEIQTVGEQENGDEAPGV
WMIPTSDPAANLHPAKPKDFSAFINLVEFCREILPEKQAEFFEPWVYSFSYELILQSTRL
PLISGFYKLLSITVRNAKKIKYFEGVSPKSLKHSPEDPEKYSCFALFVKFGKEVAVKMKQ
YKDELLASCLTFLLSLPHNIIELDVRAYVPALQMAFKLGLSYTPLAEVGLNALEEWSIYI
DRHVMQPYYKDILPCLDGYLKTSALSDETKNNWEVSALSRAAQKGFNKVVLKHLKKTKNL
SSNEAISLEEIRIRVVQMLGSLGGQINKNLLTVTSSDEMMKSYVAWDREKRLSFAVPFRE
MKPVIFLDVFLPRVTELALTASDRQTKVAACELLHSMVMFMLGKATQMPEGGQGAPPMYQ
LYKRTFPVLLRLACDVDQVTRQLYEPLVMQLIHWFTNNKKFESQDTVALLEAILDGIVDP
VDSTLRDFCGRCIREFLKWSIKQITPQQQEKSPVNTKSLFKRLYSLALHPNAFKRLGASL
AFNNIYREFREEESLVEQFVFEALVIYMESLALAHADEKSLGTIQQCCDAIDHLCRIIEK
KHVSLNKAKKRRLPRGFPPSASLCLLDLVKWLLAHCGRPQTECRHKSIELFYKFVPLLPG
NRSPNLWLKDVLKEEGVSFLINTFEGGGCGQPSGILAQPTLLYLRGPFSLQATLCWLDLL
LAALECYNTFIGERTVGALQVLGTEAQSSLLKAVAFFLESIAMHDIIAAEKCFGTGAAGN
RTSPQEGERYNYSKCTVVVRIMEFTTTLLNTSPEGWKLLKKDLCNTHLMRVLVQTLCEPA
SIGFNIGDVQVMAHLPDVCVNLMKALKMSPYKDILETHLREKITAQSIEELCAVNLYGPD
AQVDRSRLAAVVSACKQLHRAGLLHNILPSQSTDLHHSVGTELLSLVYKGIAPGDERQCL
PSLDLSCKQLASGLLELAFAFGGLCERLVSLLLNPAVLSTASLGSSQGSVIHFSHGEYFY
SLFSETINTELLKNLDLAVLELMQSSVDNTKMVSAVLNGMLDQSFRERANQKHQGLKLAT
TILQHWKKCDSWWAKDSPLETKMAVLALLAKILQIDSSVSFNTSHGSFPEVFTTYISLLA
DTKLDLHLKGQAVTLLPFFTSLTGGSLEELRRVLEQLIVAHFPMQSREFPPGTPRFNNYV
DCMKKFLDALELSQSPMLLELMTEVLCREQQHVMEELFQSSFRRIARRGSCVTQVGLLES
VYEMFRKDDPRLSFTRQSFVDRSLLTLLWHCSLDALREFFSTIVVDAIDVLKSRFTKLNE
STFDTQITKKMGYYKILDVMYSRLPKDDVHAKESKINQVFHGSCITEGNELTKTLIKLCY
DAFTENMAGENQLLERRRLYHCAAYNCAISVICCVFNELKFYQGFLFSEKPEKNLLIFEN
LIDLKRRYNFPVEVEVPMERKKKYIEIRKEAREAANGDSDGPSYMSSLSYLADSTLSEEM
SQFDFSTGVQSYSYSSQDPRPATGRFRRREQRDPTVHDDVLELEMDELNRHECMAPLTAL
VKHMHRSLGPPQGEEDSVPRDLPSWMKFLHGKLGNPIVPLNIRLFLAKLVINTEEVFRPY
AKHWLSPLLQLAASENNGGEGIHYMVVEIVATILSWTGLATPTGVPKDEVLANRLLNFLM
KHVFHPKRAVFRHNLEIIKTLVECWKDCLSIPYRLIFEKFSGKDPNSKDNSVGIQLLGIV
MANDLPPYDPQCGIQSSEYFQALVNNMSFVRYKEVYAAAAEVLGLILRYVMERKNILEES
LCELVAKQLKQHQNTMEDKFIVCLNKVTKSFPPLADRFMNAVFFLLPKFHGVLKTLCLEV
VLCRVEGMTELYFQLKSKDFVQVMRHRDDERQKVCLDIIYKMMPKLKPVELRELLNPVVE
FVSHPSTTCREQMYNILMWIHDNYRDPESETDNDSQEIFKLAKDVLIQGLIDENPGLQLI
IRNFWSHETRLPSNTLDRLLALNSLYSPKIEVHFLSLATNFLLEMTSMSPDYPNPMFEHP
LSECEFQEYTIDSDWRFRSTVLTPMFVETQASQGTLQTRTQEGSLSARWPVAGQIRATQQ
QHDFTLTQTADGRSSFDWLTGSSTDPLVDHTSPSSDSLLFAHKRSERLQRAPLKSVGPDF
GKKRLGLPGDEVDNKVKGAAGRTDLLRLRRRFMRDQEKLSLMYARKGVAEQKREKEIKSE
LKMKQDAQVVLYRSYRHGDLPDIQIKHSSLITPLQAVAQRDPIIAKQLFSSLFSGILKEM
DKFKTLSEKNNITQKLLQDFNRFLNTTFSFFPPFVSCIQDISCQHAALLSLDPAAVSAGC
LASLQQPVGIRLLEEALLRLLPAELPAKRVRGKARLPPDVLRWVELAKLYRSIGEYDVLR
GIFTSEIGTKQITQSALLAEARSDYSEAAKQYDEALNKQDWVDGEPTEAEKDFWELASLD
CYNHLAEWKSLEYCSTASIDSENPPDLNKIWSEPFYQETYLPYMIRSKLKLLLQGEADQS
LLTFIDKAMHGELQKAILELHYSQELSLLYLLQDDVDRAKYYIQNGIQSFMQNYSSIDVL
LHQSRLTKLQSVQALTEIQEFISFISKQGNLSSQVPLKRLLNTWTNRYPDAKMDPMNIWD
DIITNRCFFLSKIEEKLTPLPEDNSMNVDQDGDPSDRMEVQEQEEDISSLIRSCKFSMKM
KMIDSARKQNNFSLAMKLLKELHKESKTRDDWLVSWVQSYCRLSHCRSRSQGCSEQVLTV
LKTVSLLDENNVSSYLSKNILAFRDQNILLGTTYRIIANALSSEPACLAEIEEDKARRIL
ELSGSSSEDSEKVIAGLYQRAFQHLSEAVQAAEEEAQPPSWSCGPAAGVIDAYMTLADFC
DQQLRKEEENASVIDSAELQAYPALVVEKMLKALKLNSNEARLKFPRLLQIIERYPEETL
SLMTKEISSVPCWQFISWISHMVALLDKDQAVAVQHSVEEITDNYPQAIVYPFIISSESY
SFKDTSTGHKNKEFVARIKSKLDQGGVIQDFINALDQLSNPELLFKDWSNDVRAELAKTP
VNKKNIEKMYERMYAALGDPKAPGLGAFRRKFIQTFGKEFDKHFGKGGSKLLRMKLSDFN
DITNMLLLKMNKDSKPPGNLKECSPWMSDFKVEFLRNELEIPGQYDGRGKPLPEYHVRIA
GFDERVTVMASLRRPKRIIIRGHDEREHPFLVKGGEDLRQDQRVEQLFQVMNGILAQDSA
CSQRALQLRTYSVVPMTSRLGLIEWLENTVTLKDLLLNTMSQEEKAAYLSDPRAPPCEYK
DWLTKMSGKHDVGAYMLMYKGANRTETVTSFRKRESKVPADLLKRAFVRMSTSPEAFLAL
RSHFASSHALICISHWILGIGDRHLNNFMVAMETGGVIGIDFGHAFGSATQFLPVPELMP
FRLTRQFINLMLPMKETGLMYSIMVHALRAFRSDPGLLTNTMDVFVKEPSFDWKNFEQKM
LKKGGSWIQEINVAEKNWYPRQKICYAKRKLAGANPAVITCDELLLGHEKAPAFRDYVAV
ARGSKDHNIRAQEPESGLSEETQVKCLMDQATDPNILGRTWEGWEPWM
Sequence of entity 2 (C), FASTA
>7TYR_2 Protein artemis (chains C)
MSSFEGQMAEYPTISIDRFDRENLRARAYFLSHCHKDHMKGLRAPTLKRRLECSLKVYLY
CSPVTKELLLTSPKYRFWKKRIISIEIETPTQISLVDEASGEKEEIVVTLLPAGHCPGSV
MFLFQGNNGTVLYTGDFRLAQGEAARMELLHSGGRVKDIQSVYLDTTFCDPRFYQIPSRE
ECLSGVLELVRSWITRSPYHVVWLNCKAAYGYEYLFTNLSEELGVQVHVNKLDMFRNMPE
ILHHLTTDRNTQIHACRHPKAEEYFQWSKLPCGITSRNRIPLHIISIKPSTMWFGERSRK
TNVIVRTGESSYRACFSFHSSYSEIKDFLSYLCPVNAYPNVIPVGTTMDKVVEILKPLCR
SSQSTEPKYKPLGKLKRARTVHRDSEEEDDYLFDDPLPIPLRHKVPYPETFHPEVFSMTA
VSEKQPEKLRQTPGCCRAECMQSSRFTNFVDCEESNSESEEEVGIPASLQGDLGSVLHLQ
KADGDVPQWEVFFKRNDEITDESLENFPSSTVAGGSQSPKLFSDSDGESTHISSQNSSQS
THITEQGSQGWDSQSDTVLLSSQERNSGDITSLDKADYRPTIKENIPASLMEQNVICPKD
TYSDLKSRDKDVTIVPSTGEPTTLSSETHIPEEKSLLNLSTNADSQSSSDFEVPSTPEAE
LPKREHLQYLYEKLATGESIAVKKRKCSLLDTENLYFQGHHHHHHHH

Primary citation

Structural analysis of the basal state of the Artemis:DNA-PKcs complex. Watanabe, G., Lieber, M.R., Williams, D.R. Nucleic Acids Res (2022) 50:7697-7720. DOI 10.1093/nar/gkac564 · PubMed

Other PDB entries of the same protein (UniProt P78527), best resolution first:

Browse structure collections

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