7UC9: Alcohol dehydrogenase E chain

Horse liver alcohol dehydrogenase with NAD and trifluoroethanol at 45 K. Determined by X-ray diffraction at 1.1 Å resolution. Released 30 Mar 2022.

Method
X-ray diffraction
Resolution
1.1 Å
Organism
Equus caballus
Chains
2
Atoms
6,983
Mol. weight
81.97 kDa
Ligands
ZN, NAJ, ETF, MRD
Released
30 Mar 2022

Explore 7UC9 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7UC9 contains 44 α-helices and 46 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 21 helices, 22 β-strands

ElementResiduesLengthSheet
β-strand7-1481
β-strand22-2871
α-helix29-313
β-strand35-44102
α-helix47-548
β-strand63-6421
β-strand69-7682
β-strand88-9142
α-helix101-1044
β-strand130-13231
β-strand135-13841
β-strand14711
β-strand149-15352
α-helix154-1563
β-strand157-15932
α-helix166-1694
α-helix170-1734
α-helix175-1817
α-helix182-1876
β-strand194-19853
α-helix202-21312
β-strand218-22253
α-helix226-2283
α-helix229-2357
β-strand239-24133
α-helix243-2453
α-helix250-2578
β-strand26214
β-strand264-26743
α-helix272-28110
β-strand28214
β-strand288-29143
α-helix299-3002
β-strand301-30335
α-helix306-3094
β-strand313-31643
α-helix319-3213
α-helix324-33613
α-helix343-3453
β-strand346-35162
α-helix352-3543
α-helix355-3639
β-strand369-37352
Chain B: 23 helices, 24 β-strands
ElementResiduesLengthSheet
α-helix61
β-strand7-1376
β-strand1417
β-strand22-2876
α-helix29-313
β-strand35-44108
α-helix47-548
β-strand6317
β-strand68-7698
β-strand88-9148
α-helix101-1044
β-strand130-13236
β-strand135-13736
β-strand13817
β-strand14716
β-strand149-15358
α-helix154-1563
β-strand157-15938
α-helix166-1694
α-helix170-1734
α-helix175-1817
α-helix182-1876
α-helix189-1902
β-strand194-19853
α-helix202-21312
β-strand218-22253
α-helix226-2283
α-helix229-2357
β-strand239-24133
α-helix243-2453
α-helix250-2578
β-strand26219
β-strand264-26743
α-helix272-28110
β-strand28219
β-strand288-29143
α-helix299-3002
β-strand301-30335
α-helix306-3094
β-strand313-31643
α-helix319-3213
α-helix324-33613
α-helix343-3453
β-strand346-35168
α-helix352-3543
α-helix355-3639
β-strand369-37358

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Alcohol dehydrogenase E chainA, Bprotein374Equus caballusP00327 (AlphaFold model)
Sequence of entity 1 (A, B), FASTA
>7UC9_1 Alcohol dehydrogenase E chain (chains A, B)
STAGKVIKCKAAVLWEEKKPFSIEEVEVAPPKAHEVRIKMVATGICRSDDHVVSGTLVTP
LPVIAGHEAAGIVESIGEGVTTVRPGDKVIPLFTPQCGKCRVCKHPEGNFCLKNDLSMPR
GTMQDGTSRFTCRGKPIHHFLGTSTFSQYTVVDEISVAKIDAASPLEKVCLIGCGFSTGY
GSAVKVAKVTQGSTCAVFGLGGVGLSVIMGCKAAGAARIIGVDINKDKFAKAKEVGATEC
VNPQDYKKPIQEVLTEMSNGGVDFSFEVIGRLDTMVTALSCCQEAYGVSVIVGVPPDSQN
LSMNPMLLLSGRTWKGAIFGGFKSKDSVPKLVADFMAKKFALDPLITHVLPFEKINEGFD
LLRSGESIRTILTF

Ligands and cofactors

IDNameFormulaCopies
ZNZinc ionZn4
NAJNicotinamide-adenine-dinucleotide (acidic form)C21 H27 N7 O14 P22
ETFTrifluoroethanolC2 H3 F3 O2
MRD(4R)-2-methylpentane-2,4-diolC6 H14 O24

Primary citation

Dependence of crystallographic atomic displacement factors on temperature (25-150 K) for complexes of horse liver alcohol dehydrogenases. Plapp, B.V., Gakhar, L., Subramanian, R. Acta Crystallogr D Biol Crystallogr (2022) D78:1221-1234. DOI 10.1107/S2059798322008361

Other PDB entries of the same protein (UniProt P00327 (AlphaFold model), which also has an AlphaFold model), best resolution first:

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