7UDT: Rigor state wild type myosin-15-F-actin complex

cryo-EM structure of the rigor state wild type myosin-15-F-actin complex (symmetry expansion and re-centering). Determined by electron microscopy at 3.17 Å resolution. Released 3 Aug 2022.

Method
Electron microscopy
Resolution
3.17 Å
Organisms
Gallus gallus, Mus musculus
Chains
5
Atoms
15,806
Mol. weight
225.58 kDa
Ligands
ADP, MG
Released
3 Aug 2022

Explore 7UDT in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7UDT contains 109 α-helices and 80 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 25 helices, 20 β-strands

ElementResiduesLengthSheet
β-strand8-1252
β-strand16-2162
β-strand29-3242
β-strand35-3843
β-strand41-4224
β-strand53-5423
α-helix56-605
β-strand65-6843
β-strand71-7225
β-strand75-7625
α-helix79-879
α-helix88-936
α-helix98-1003
β-strand103-10752
α-helix113-12513
β-strand131-13662
α-helix137-1459
β-strand150-15566
β-strand160-16676
β-strand169-17026
α-helix172-1743
β-strand176-17836
α-helix182-19211
α-helix193-1964
α-helix203-21614
α-helix223-23210
β-strand238-24147
β-strand247-25047
α-helix253-2564
α-helix258-2614
α-helix264-2674
α-helix271-2733
α-helix274-28310
α-helix290-2945
β-strand297-30046
α-helix303-3053
α-helix309-32012
β-strand329-33026
α-helix335-3373
α-helix338-34710
α-helix351-3544
β-strand357-35822
α-helix359-3657
α-helix366-3683
α-helix369-3735
Chains B and C: 25 helices, 19 β-strands
ElementResiduesLengthSheet
β-strand8-1258
β-strand16-2168
β-strand29-3248
β-strand35-3849
β-strand53-5429
α-helix56-605
β-strand65-6849
β-strand71-72210
β-strand75-76210
α-helix79-879
α-helix88-936
α-helix98-1003
β-strand103-10758
α-helix113-12513
β-strand131-13668
α-helix137-1459
β-strand150-15564
β-strand160-16674
β-strand169-17024
α-helix172-1743
β-strand176-17834
α-helix182-19211
α-helix193-1964
α-helix203-21614
α-helix223-23210
β-strand238-241411
β-strand247-250411
α-helix253-2564
α-helix258-2614
α-helix264-2674
α-helix271-2733
α-helix274-28310
α-helix290-2945
β-strand297-30044
α-helix303-3053
α-helix309-32012
β-strand329-33024
α-helix335-3373
α-helix338-34710
α-helix351-3544
β-strand357-35828
α-helix359-3657
α-helix366-3683
α-helix369-3735
Chain D: 27 helices, 20 β-strands
ElementResiduesLengthSheet
β-strand1210117
α-helix1211-12133
α-helix1219-123113
β-strand1237-1239317
β-strand1242-1246517
α-helix1257-12626
α-helix1275-128915
β-strand1293-1299717
α-helix1305-131915
α-helix1326-13327
α-helix1335-13428
β-strand1343-1344218
β-strand1352-1353218
β-strand1356-1364917
β-strand1367-13761017
β-strand1393118
α-helix1394-14029
α-helix1405-14117
α-helix1435-144814
α-helix1453-146917
β-strand1474-1478519
β-strand1483-1487519
α-helix1491-150010
α-helix1504-15129
β-strand1513-1517520
β-strand1522-1526520
α-helix1529-155830
β-strand1566-1572717
α-helix1584-161431
α-helix1628-16358
α-helix1641-16488
α-helix1656-166712
β-strand1673-1674221
β-strand1682-1687621
β-strand1690-1695621
β-strand1704122
α-helix1709-17168
α-helix1721-173313
β-strand1753122
α-helix1754-176714
β-strand1775-1780617
α-helix1793-180311
α-helix1806-18127
α-helix1823-18286
α-helix1847-18548
α-helix1872-190938
α-helix1914-19218
Chain G: 7 helices, 2 β-strands
ElementResiduesLengthSheet
α-helix28-4114
α-helix51-588
α-helix83-9210
α-helix100-10910
β-strand118-11921
α-helix120-12910
α-helix136-1438
β-strand153-15421
α-helix157-1659

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
regulatory light chainGprotein146Gallus gallusP24032 (AlphaFold model)
Actin, alpha skeletal muscleA, B, Cprotein373Gallus gallusP68139 (AlphaFold model)
Unconventional myosin-XVDprotein719Mus musculusQ9QZZ4 (AlphaFold model)
Sequence of entity 1 (G), FASTA
>7UDT_1 regulatory light chain (chains G)
VFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDEYLDAMMNEAPGPI
NFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGFIQEDYLRELLTTMGDRFTDEEVDE
LYREAPIDKKGNFNYIEFTRILKHGA
Sequence of entity 2 (A, B, C), FASTA
>7UDT_2 Actin, alpha skeletal muscle (chains A, B, C)
DETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR
GILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQI
MFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAG
RDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYEL
PDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMSGG
TTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEY
DEAGPSIVHRKCF
Sequence of entity 3 (D), FASTA
>7UDT_3 Unconventional myosin-XV (chains D)
EDGVEDMTQLEDLQETTVLANLKTRFERNLIYTYIGSILVSVNPYRMFAIYGPEQVQQYS
GRALGENPPHLFAIANLAFAKMLDAKQNQCVIISGESGSGKTEATKLILRCLAAMNQRRD
VMQQIKILEATPLLEAFGNAKTVRNDNSSRFGKFVEIFLEGGVICGAITSQYLLEKSRIV
FQAKNERNYHIFYELLAGLPAQLRQAFSLQEAETYYYLNQGGNCEIAGKSDADDFRRLLA
AMEVLGFTSEDQDSIFRILASILHLGNVYFEKHETDAQEVASVVSAREIQAVAELLQVSP
EGLQKAITFKVTETIREKIFTPLTVESAVDARDAIAKVLYALLFGWLITRVNALVSPKQD
TLSIAILDIYGFEDLSFNSFEQLCINYANENLQYLFNKIVFQEEQEEYIREQMDWREIAF
ADNQPCINLISLKPYGILRILDDQCCFPQATDHTFLQKCHYHHGANPLYSKPKMPLPEFT
IKHYAGKVTYQVHKFLDKNHDQVRQDVLDLFVHSRTRVVAHLFSSHAAQTAPPRLGKSSS
ITRLYKAHTVAAKFQQSLLDLVEKMERCNPLFVRCLKPNHKKEPGLFEPDVMMAQLRYSG
VLETVRIRKEGFPVRLPFQVFIDRYRCLVALKLNVPADGDMCVSLLSRLCTVTPDMYRVG
ISKLFLKEHLHQLLESMRERVQNRAALTLQRYLRGFFIQRHFRSLRRKIILLQSRARGF

Ligands and cofactors

IDNameFormulaCopies
ADPAdenosine-5'-diphosphateC10 H15 N5 O10 P23
MGMagnesium ionMg3

Primary citation

Structural basis for tunable control of actin dynamics by myosin-15 in mechanosensory stereocilia. Gong, R., Jiang, F., Moreland, Z.G. et al. Sci Adv (2022) 8:eabl4733-eabl4733. DOI 10.1126/sciadv.abl4733 · PubMed

Other PDB entries of the same protein (UniProt P24032 (AlphaFold model), which also has an AlphaFold model), best resolution first:

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