7UIC: Mediator-PIC Early

Mediator-PIC Early (Tail A). Determined by electron microscopy at 3.7 Å resolution. Released 15 Feb 2023.

Method
Electron microscopy
Resolution
3.7 Å
Organism
Saccharomyces cerevisiae S288C
Chains
6
Atoms
17,303
Mol. weight
575.15 kDa
Released
15 Feb 2023

Explore 7UIC in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7UIC contains 104 α-helices and 65 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain b: 2 helices, 0 β-strands

ElementResiduesLengthSheet
α-helix29-5022
α-helix67-10337
Chain c: 6 helices, 0 β-strands
ElementResiduesLengthSheet
α-helix11-188
α-helix21-233
α-helix24-5532
α-helix64-9431
α-helix96-994
α-helix101-1099
Chain e: 43 helices, 2 β-strands
ElementResiduesLengthSheet
α-helix288-30417
α-helix313-32412
α-helix335-35521
α-helix368-3758
α-helix376-3805
α-helix381-3866
α-helix393-4019
α-helix405-41814
α-helix439-44911
α-helix458-4614
β-strand479-48241
β-strand488-49141
α-helix494-50411
α-helix507-5093
α-helix521-5277
α-helix529-5313
α-helix534-55421
α-helix557-56913
α-helix574-5796
α-helix584-59714
α-helix600-6034
α-helix612-63625
α-helix641-6477
α-helix657-6637
α-helix683-69715
α-helix698-7025
α-helix707-7126
α-helix715-73521
α-helix742-7498
α-helix750-7523
α-helix757-7604
α-helix761-77616
α-helix781-79515
α-helix805-81410
α-helix816-8249
α-helix847-85812
α-helix868-8714
α-helix895-90814
α-helix917-92711
α-helix929-94618
α-helix959-97416
α-helix981-99111
α-helix1079-10824
α-helix1088-110518
α-helix1110-112920
Chain n: 6 helices, 10 β-strands
ElementResiduesLengthSheet
α-helix835-84915
β-strand856-86052
β-strand864-86852
β-strand880-88672
β-strand891-89882
α-helix903-9075
α-helix908-9136
α-helix918-94225
β-strand956-96383
β-strand966-97383
β-strand978-991143
β-strand994-100293
α-helix1016-102510
β-strand105013
β-strand1054-105743
α-helix1059-107315
Chain o: 5 helices, 2 β-strands
ElementResiduesLengthSheet
α-helix849-87527
α-helix880-89213
α-helix907-9148
α-helix920-9223
α-helix923-9319
β-strand938-93924
β-strand942-94324
Chain p: 42 helices, 51 β-strands
ElementResiduesLengthSheet
β-strand8-1035
β-strand16-1945
β-strand28-3145
β-strand33-3536
β-strand41-4336
α-helix44-463
β-strand47-4935
β-strand101-10227
β-strand105-11068
β-strand120-12568
β-strand130-13568
β-strand148-15478
β-strand159-16138
α-helix173-1742
β-strand178-17927
β-strand190-19569
β-strand197110
β-strand202-2111011
β-strand216-224911
β-strand230110
β-strand235-24399
β-strand247-25489
β-strand260-26679
α-helix273-2764
β-strand281-288812
β-strand294-301812
β-strand306-3151012
α-helix327-3293
β-strand330-3391012
α-helix340-3423
β-strand344113
β-strand350113
β-strand353-358614
β-strand371-377714
β-strand384-392914
β-strand426-4351014
β-strand439-444615
α-helix4451
β-strand450-455615
β-strand460-464515
β-strand470115
α-helix474-4774
β-strand485-488411
α-helix491-4933
β-strand497-498216
α-helix501-5033
α-helix504-5063
β-strand507-512617
β-strand518-523617
β-strand532-533217
β-strand534-535216
α-helix542-5443
α-helix545-56117
α-helix567-58317
α-helix585-60218
α-helix611-6188
α-helix623-63311
α-helix641-67535
α-helix683-72240
α-helix727-7293
α-helix731-7377
α-helix739-76123
α-helix768-7703
α-helix771-78212
α-helix788-80821
α-helix813-8164
α-helix817-8215
α-helix826-8283
α-helix829-84214
α-helix844-8463
α-helix849-8546
α-helix864-8663
β-strand868118
α-helix879-8813
β-strand88416
α-helix887-8893
α-helix892-8943
α-helix897-8993
β-strand901118
β-strand905119
β-strand912119
α-helix913-9142
α-helix915-9195
β-strand924-926320
β-strand932-933220
α-helix938-9403
α-helix941-9433
α-helix955-9595
β-strand962121
α-helix9671
β-strand968121
β-strand969-971320
α-helix972-9732

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Mediator of RNA polymerase II transcription subunit 2bprotein431Saccharomyces cerevisiae S288CQ12124 (AlphaFold model)
Mediator of RNA polymerase II transcription subunit 3cprotein397Saccharomyces cerevisiae S288CP40356 (AlphaFold model)
Mediator of RNA polymerase II transcription subunit 5eprotein1132Saccharomyces cerevisiae S288CP53114 (AlphaFold model)
Mediator of RNA polymerase II transcription subunit 14nprotein1082Saccharomyces cerevisiae S288CP19263 (AlphaFold model)
Mediator of RNA polymerase II transcription subunit 15oprotein1081Saccharomyces cerevisiae S288CP19659
Mediator of RNA polymerase II transcription subunit 16pprotein974Saccharomyces cerevisiae S288CP32259
Sequence of entity 1 (b), FASTA
>7UIC_1 Mediator of RNA polymerase II transcription subunit 2 (chains b)
MVVQNSPVSSVHTANFSERGSNTRTMTYKNKLTVCFDDILKVGAEMMMQQQLKNVQLDSY
LVNGFSQSQQKLLKEKVKLFHGILDDLETSLSQSSSYLETLTALGKEKEKEREEAEKKRA
EQENMRKVREQEELKKRQELEEASQQQQLQQNSKEKNGLGLNFSTTAPANTTDANGSKEN
YQELGSLQSSSQTQLENANAANNGAAFSPLTTTRIQSQQAQPSDVMFNDLNSMDISMFSG
LDSTGFDSTAFNATVDETKGFDDNDSGNNYNDINISSIENNINNNINSTKNGKDNNNESN
KNNNGDEKNKNNNEDNENNNNSSEKNNNNNNNNNNNNDDNGNNNNNNSGNDNNNTTNNDS
NNKNNSITTGNDNENIVNNDLPTTVVSNPGDNPPPADNGEEYLTLNDFNDLNIDWSTTGD
NGELDLSGFNI
Sequence of entity 2 (c), FASTA
>7UIC_2 Mediator of RNA polymerase II transcription subunit 3 (chains c)
MDSIIPAGVKLDDLQVILAKNENETRDKVCKQINEARDEILPLRLQFNEFIQIMANIDQE
GSKQADRMAKYLHIRDKILQLNDRFQTLSSHLEALQPLFSTVPEYLKTADNRDRSFQLLE
PLSTYNKNGNAVCSTATVVSTNHSAAASTPTTTATPHANPITHAHSLSNPNSTATMQHNP
LAGKRGPKSGSTMGTPTVHNSTAAAPIAAPKKPRKPRQTKKAKAQAQAQAQAQAQVYAQQ
STVQTPITASMAAALPNPTPSMINSVSPTNVMGTPLTNMMSPMGNAYSMGAQNQGGQVSM
SQFNGSGNGSNPNTNTNSNNTPLQSQLNLNNLTPANILNMSMNNDFQQQQQQQQQQQQPQ
PQYNMNMGMNNMNNGGKELDSLDLNNLELGGLNMDFL
Sequence of entity 3 (e), FASTA
>7UIC_3 Mediator of RNA polymerase II transcription subunit 5 (chains e)
MEKESVYNLALKCAERQLTSMEFSNLYKEFFNEKFPSLIQEEEEDTTTTANINEVKKASD
LVDTPSNNTAATADTTHLHEALDIVCSDFVKILNLEKPLILADYIVEVLLVNYNSDMIKC
FLPKLNSVRNSLLLAHFFSKSCSFFAKLSDTLIIDQVRKDLGNVIVPNILSLDMNSMNKE
LIAIVSKLLQTTLKLSPSPILLTSAGCKNGSFTLLNQLSQTNKLLFKRVSQTFEAKLHFK
DTKPFLNKDSTNEFVGSPSLTSPQYIPSPLSSTKPPGSVNSAAKYKDMKLLRYYKNIWLN
NKIINWEISNPDFLSKYSAITSSIFQESFNSVQNLDQLLTDLIETSFTCFAQFVSNKQYH
QANSNLTLLERKWVIFITKHLPLLILENSSRSPRVVTNALDNIDEKVVKAIRIYFTEKDD
NKTNNEDLFDDYPSTSLDIRHDFIKGLIMLNLQPASVINNYLREDQMIDTSILPTRDDLF
VRNLQGIQEVVHNTNSFIISSLDTLELESITESITHDSSNGLFQVLHNFESVAPTKQREI
VKAFLSIFEDAIKELNYNRIAKICALLFFNFSHSLTTILSFSSPAALMKTLIKFVDLSRN
GRNGSNGNDESSEYETINISLSFSWAILLIINLTQTYGISVVDVALKYPELSIKNSFIIN
FISNLPNVSDKYYLEESNVNDSDMLTKSHNTVQSWLCDLFVNGSITDQLIQNIETRQLAN
LIPFIVKQVLLSVEIGVLTDISSLIGGFEYFLQPLLLVGLIKTFYWLEQFLSCVKNDTIS
EDILQGIFNLLNTLFNPVTLNEDSKAFHTAVLRLNAIPLLKVLRKFRVQSQSNYGIYSSD
AQGDPNLEPLIAKLVAVLNVSPVYDVDPRIINSENDYSRKQLGYGKFLILNENPINKIMT
NQINSFWSLHSSTYYNLDYLFELIELVTPKSFLFDVLKTLEYKLATYGVPGSENKRGSLD
SEHVFDYFFYFLVLYDVKTAEEASQLIEYMENDAKKSKGDVDIKGEDLHEKNDSAEVRQE
TQPKAEATQDDDFDMLFGENDTSTQAYEEEEENEDNDGNNRTNNVPMIKAEETPSKTNKI
SILKRHSFAVLLHERKLLNDLALENGEITKTENEKFISYHDKYLCMLKTCVF
Sequence of entity 4 (n), FASTA
>7UIC_4 Mediator of RNA polymerase II transcription subunit 14 (chains n)
MTTTIGSPQMLANEERLSNEMHALKNRSEQNGQEQQGPVKNTQLHGPSATDPETTATQKE
SLEMVPKDTSAATMTSAPPPALPHVEINQVSLALVIRNLTVFTMKELAQYMKTNVHTQAN
EPNSAKKIRFLQLIIFLRTQFLKLYVLVKWTRTIKQNNFHVLIDLLNWFRTTNMNVNNCI
WALKSSLNSMTNAKLPNVDLVTALEVLSLGRPNLPTHNFKLSGVSNSMDMVDGMAKVPIG
LILQRLKDLNLTVSIKIALMNIPKPLNSYHIKNGRIYFTVPNEFEIQLSTVNRQSPLFFV
DLKLLFNTEAEQTVSAVTEATSTNGDSENNEENSSSNGNNLPLNKPRLEKLINEILLKSN
DPLLSLYNFLHKYVLTLQLYMVHREFLKLANGGKFSKSNLIHNYDSKKSTITVRYWLNGK
MDSKGKITIGIQRTTESLILKWDNQSASRAKNMPVIYNNIVSNIEGILDEIMFNHARIIR
SELLARDIFQEDEENSDVLLFQLPTTCVSMAPIQLKIDLLSGQFYFRNPTPLLSNYASKI
NRAEGPEELARILQQLKLDKIIHVLTTMFENTGWSCSRIIKIDKPIRTQVNTGGESVVKK
EDNKYAIAGNSTTNSDVSLLLQRDLFIRLPHWPLNWYLILSIISSKTSCVVEKRIGKIVS
QRGKWNLKYLDNSNVMTVKLESITYQKIMILQRTILNRIINHMLIDSLNQLEIRNKICSS
EMINEQKLPQYIIQGSNTNDNISIITLELESFLEGSKALNSILESSMFLRIDYSNSQIRL
YAKFKRNTMMIQCQIDKLYIHFVQEEPLAFYLEESFTNLGIIVQYLTKFRQKLMQLVVLT
DVVERLHKNFESENFKIIALQPNEISFKYLSNNDEDDKDCTIKISTNDDSIKNLTVQLSP
SNPQHIIQPFLDNSKMDYHFIFSYLQFTSSLFKALKVILNERGGKFHESGSQYSTMVNIG
LHNLNEYQIVYYNPQAGTKITICIELKTVLHNGRDKIQFHIHFADVAHITTKSPAYPMMH
QVRNQVFMLDTKRLGTPESVKPANASHAIRLGNGVACDPSEIEPILMEIHNILKVDSNSS
SS
Sequence of entity 5 (o), FASTA
>7UIC_5 Mediator of RNA polymerase II transcription subunit 15 (chains o)
MSAAPVQDKDTLSNAERAKNVNGLLQVLMDINTLNGGSSDTADKIRIHAKNFEAALFAKS
SSKKEYMDSMNEKVAVMRNTYNTRKNAVTAAAANNNIKPVEQHHINNLKNSGNSANNMNV
NMNLNPQMFLNQQAQARQQVAQQLRNQQQQQQQQQQQQRRQLTPQQQQLVNQMKVAPIPK
QLLQRIPNIPPNINTWQQVTALAQQKLLTPQDMEAAKEVYKIHQQLLFKARLQQQQAQAQ
AQANNNNNGLPQNGNINNNINIPQQQQMQPPNSSANNNPLQQQSSQNTVPNVLNQINQIF
SPEEQRSLLQEAIETCKNFEKTQLGSTMTEPVKQSFIRKYINQKALRKIQALRDVKNNNN
ANNNGSNLQRAQNVPMNIIQQQQQQNTNNNDTIATSATPNAAAFSQQQNASSKLYQMQQQ
QQAQAQAQAQAQAQAQAQAQAQAAQAAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQ
AHAQHQPSQQPQQAQQQPNPLHGLTPTAKDVEVIKQLSLDASKTNLRLTDVTNSLSNEEK
EKIKMKLKQGQKLFVQVSNFAPQVYIITKNENFLKEVFQLRIFVKEILEKCAEGIFVVKL
DTVDRLIIKYQKYWESMRIQILRRQAILRQQQQMANNNGNPGTTSTGNNNNIATQQNMQQ
SLQQMQHLQQLKMQQQQQQQQQQQQQQQQQQQQQQQHIYPSSTPGVANYSAMANAPGNNI
PYMNHKNTSSMDFLNSMENTPKVPVSAAATPSLNKTINGKVNGRTKSNTIPVTSIPSTNK
KLSISNAASQQPTPRSASNTAKSTPNTNPSPLKTQTKNGTPNPNNMKTVQSPMGAQPSYN
SAIIENAFRKEELLLKDLEIRKLEISSRFKHRQEIFKDSPMDLFMSTLGDCLGIKDEEML
TSCTIPKAVVDHINGSGKRKPTKAAQRARDQDSIDISIKDNKLVMKSKFNKSNRSYSIAL
SNVAAIFKGIGGNFKDLSTLVHSSSPSTSSNMDVGNPRKRKASVLEISPQDSIASVLSPD
SNIMSDSKKIKVDSPDDPFMTKSGATTSEKQEVTNEAPFLTSGTSSEQFNVWDWNNWTSA
T
Sequence of entity 6 (p), FASTA
>7UIC_6 Mediator of RNA polymerase II transcription subunit 16 (chains p)
MMLGEHLMSWSKTGIIAYSDSQSSNANICLTFLESINGINWRFHTPQKYVLHPQLHEVQY
QESSSTLSTHSTTTSVNGSTTAGVGSTPNFGGNSNKSPPQFFYNISSIHWNNWFSLPGDM
LAVCDELGNMTMLITGQRPDRATTYEKLTMVFQDNVYKIYNHVMPLKPVDKLKPMNIERK
QTRKEYNTSILEFRWLTSSKSVIVSQFCAFDSSSNTYRSRAQQVPPYGVYHPPFIKYACL
AIRKNGQIDFWYQFSNSKDHKKITLQLLDTSNQRFKDLQWLEFARITPMNDDQCMLITTY
SKLSKNISFYKLHVNWNLNATKPNVLNDPSLKIQFILSTTLDPTDDEGHVLKLENLHVVS
KSSIEKDPSPEILVLYNVCDTSKSLVKRYRLAPTQLSAEYLVILKPDLNIDRNNSTNQIF
QSRRYNLRRHSDIVLDKKVTLITSEMFDAFVSFYFEDGTIESYNQNDWKLETERLISQSQ
LGKFKNIIASPLSAGFNYGKLPLPPSVEWMKVSPSMCGVIVKQYNKKWPQFYAAVQKNYA
DPEKDSINATALAFGYVKSLHKQISAEDLTIAAKTHILRISFLDRKRAKEFITTLLKSLY
SFFNISPDAPKEIMDKIITSRPLQKIMLLQLELGSCFSQENIEEMARVILYLKNVLFAFN
GVARNFHFAIEQISNNSNQQQNPKLFQTIFSKQDLIHSLIPVAKWFVKFITYLTQEILIL
INDPTNKEYTLVHGIFGAKMSRTLILSILNEIKKVTQIVAKFPETSYPILNESSTFLKLV
LSESPVDFEKFETFLVDVNNKFIALCEQQPSQEREFSLLVKAEIPPEYAKVGDFLLQYAN
NAVISHANAAAVYFADTSGLKISNSEFFNPEIFHLLQPLEEGLIIDTDKLPIKNRTSKSF
SKLLYDDVTCDKLSVSEISDGKLKRCSRCGSVTRAGNIISSDKTIVPTSIQTKRWPTMYT
RLCICSGMLFEMDG

Primary citation

Structural basis of a transcription pre-initiation complex on a divergent promoter. Gorbea Colon, J.J., Palao 3rd, L., Chen, S.F. et al. Mol Cell (2023) 83:574. DOI 10.1016/j.molcel.2023.01.011 · PubMed

Other PDB entries of the same protein (UniProt Q12124 (AlphaFold model), which also has an AlphaFold model), best resolution first:

Browse structure collections

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