7UIO: Mediator-PIC Early
Mediator-PIC Early (Composite Model). Determined by electron microscopy at 3.3 Å resolution. Released 1 Mar 2023.
- Method
- Electron microscopy
- Resolution
- 3.3 Å
- Organism
- Saccharomyces cerevisiae S288C
- Chains
- 80
- Atoms
- 164,116
- Mol. weight
- 3456.49 kDa
- Ligands
- ASP, ALA, THR, ZN
- Released
- 1 Mar 2023
Explore 7UIO in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
7UIO contains 916 α-helices and 804 β-strands across 78 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chains Aa and Ba: 12 helices, 22 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 7-21 | 15 | |
| α-helix | 28-37 | 10 | |
| β-strand | 41-46 | 6 | 118 |
| β-strand | 51-57 | 7 | 118 |
| β-strand | 61-68 | 8 | 118 |
| β-strand | 73-81 | 9 | 118 |
| β-strand | 91 | 1 | 119 |
| β-strand | 102 | 1 | 119 |
| α-helix | 103-109 | 7 | |
| α-helix | 115-130 | 16 | |
| α-helix | 164-181 | 18 | |
| β-strand | 187-190 | 4 | 120 |
| β-strand | 198-203 | 6 | 120 |
| β-strand | 209-219 | 11 | 120 |
| β-strand | 227-229 | 3 | 121 |
| β-strand | 232-234 | 3 | 122 |
| β-strand | 239-241 | 3 | 122 |
| β-strand | 248-250 | 3 | 121 |
| β-strand | 252-258 | 7 | 120 |
| β-strand | 272-274 | 3 | 123 |
| α-helix | 280-282 | 3 | |
| β-strand | 283-286 | 4 | 120 |
| α-helix | 298-304 | 7 | |
| β-strand | 312-316 | 5 | 124 |
| β-strand | 322-324 | 3 | 123 |
| β-strand | 325-329 | 5 | 120 |
| α-helix | 332-336 | 5 | |
| α-helix | 337-347 | 11 | |
| α-helix | 348-352 | 5 | |
| α-helix | 353-360 | 8 | |
| β-strand | 528-532 | 5 | 124 |
| β-strand | 536-539 | 4 | 124 |
| β-strand | 543-546 | 4 | 124 |
| α-helix | 551-565 | 15 | |
Chains AA and BA: 82 helices, 71 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 10 | 1 | |
| β-strand | 11 | 1 | 175 |
| β-strand | 14-18 | 5 | 176 |
| α-helix | 24-30 | 7 | |
| β-strand | 34 | 1 | 177 |
| β-strand | 41 | 1 | 178 |
| β-strand | 48 | 1 | 178 |
| α-helix | 49 | 1 | |
| β-strand | 82-91 | 10 | 177 |
| α-helix | 96-104 | 9 | |
| β-strand | 106 | 1 | 179 |
| β-strand | 113 | 1 | 179 |
| α-helix | 120-126 | 7 | |
| α-helix | 131-142 | 12 | |
| β-strand | 147 | 1 | 180 |
| β-strand | 151-153 | 3 | 181 |
| β-strand | 161-163 | 3 | 181 |
| β-strand | 170 | 1 | 180 |
| β-strand | 173-177 | 5 | 182 |
| β-strand | 180-185 | 6 | 182 |
| α-helix | 189-191 | 3 | |
| β-strand | 198-201 | 4 | 182 |
| α-helix | 202-203 | 2 | |
| α-helix | 204-212 | 9 | |
| α-helix | 216-222 | 7 | |
| α-helix | 232-234 | 3 | |
| β-strand | 235-241 | 7 | 177 |
| α-helix | 248-249 | 2 | |
| β-strand | 250-253 | 4 | 183 |
| β-strand | 256-258 | 3 | 183 |
| α-helix | 261-280 | 20 | |
| α-helix | 286-304 | 19 | |
| α-helix | 312-314 | 3 | |
| β-strand | 315 | 1 | 184 |
| α-helix | 316 | 1 | |
| α-helix | 320 | 1 | |
| β-strand | 321 | 1 | 184 |
| α-helix | 322 | 1 | |
| α-helix | 326-329 | 4 | |
| α-helix | 335 | 1 | |
| α-helix | 336-340 | 5 | |
| β-strand | 343-345 | 3 | 185 |
| β-strand | 348-352 | 5 | 186 |
| β-strand | 355 | 1 | 187 |
| β-strand | 363-367 | 5 | 186 |
| α-helix | 368-373 | 6 | |
| β-strand | 375-379 | 5 | 188 |
| α-helix | 380 | 1 | |
| α-helix | 385-394 | 10 | |
| β-strand | 402-406 | 5 | 188 |
| β-strand | 412-414 | 3 | 188 |
| α-helix | 424-426 | 3 | |
| β-strand | 431-435 | 5 | 188 |
| α-helix | 436 | 1 | |
| β-strand | 441-445 | 5 | 186 |
| α-helix | 452-454 | 3 | |
| β-strand | 455-463 | 9 | 186 |
| β-strand | 469 | 1 | 186 |
| β-strand | 470 | 1 | 187 |
| β-strand | 486-490 | 5 | 186 |
| α-helix | 495-503 | 9 | |
| β-strand | 506 | 1 | 186 |
| α-helix | 507-509 | 3 | |
| β-strand | 512-513 | 2 | 189 |
| β-strand | 518-519 | 2 | 189 |
| α-helix | 525-534 | 10 | |
| β-strand | 540-541 | 2 | 190 |
| α-helix | 543-552 | 10 | |
| α-helix | 559-563 | 5 | |
| β-strand | 565-567 | 3 | 21 |
| β-strand | 571 | 1 | 21 |
| β-strand | 572-573 | 2 | 190 |
| α-helix | 574-578 | 5 | |
| β-strand | 588-590 | 3 | 191 |
| β-strand | 605-608 | 4 | 191 |
| β-strand | 611-614 | 4 | 191 |
| α-helix | 619-622 | 4 | |
| α-helix | 629-636 | 8 | |
| α-helix | 639-660 | 22 | |
| α-helix | 666-668 | 3 | |
| α-helix | 673-697 | 25 | |
| α-helix | 702-704 | 3 | |
| α-helix | 710-736 | 27 | |
| α-helix | 742-749 | 8 | |
| α-helix | 755-758 | 4 | |
| α-helix | 759-763 | 5 | |
| β-strand | 766-767 | 2 | 192 |
| β-strand | 770 | 1 | 193 |
| β-strand | 773 | 1 | 193 |
| α-helix | 794-796 | 3 | |
| β-strand | 799-800 | 2 | 192 |
| α-helix | 810-845 | 36 | |
| β-strand | 849-850 | 2 | 194 |
| β-strand | 856-857 | 2 | 194 |
| β-strand | 863-865 | 3 | 194 |
| α-helix | 868-870 | 3 | |
| β-strand | 873 | 1 | 195 |
| β-strand | 878-882 | 5 | 196 |
| α-helix | 890-897 | 8 | |
| α-helix | 904-906 | 3 | |
| α-helix | 907-909 | 3 | |
| β-strand | 913 | 1 | 197 |
| α-helix | 916-919 | 4 | |
| α-helix | 924-946 | 23 | |
| β-strand | 953-957 | 5 | 196 |
| α-helix | 960-970 | 11 | |
| β-strand | 979 | 1 | 197 |
| α-helix | 983-995 | 13 | |
| α-helix | 1005-1025 | 21 | |
| α-helix | 1028-1029 | 2 | |
| α-helix | 1030-1035 | 6 | |
| α-helix | 1039-1054 | 16 | |
| β-strand | 1057 | 1 | 195 |
| α-helix | 1058-1059 | 2 | |
| α-helix | 1064-1078 | 15 | |
| α-helix | 1083-1088 | 6 | |
| α-helix | 1090-1092 | 3 | |
| α-helix | 1098-1106 | 9 | |
| β-strand | 1116-1119 | 4 | 198 |
| β-strand | 1120 | 1 | 199 |
| α-helix | 1128-1138 | 11 | |
| β-strand | 1141-1142 | 2 | 200 |
| α-helix | 1143-1145 | 3 | |
| β-strand | 1147-1154 | 8 | 201 |
| α-helix | 1164-1174 | 11 | |
| α-helix | 1179-1184 | 6 | |
| α-helix | 1185-1187 | 3 | |
| β-strand | 1191-1197 | 7 | 201 |
| α-helix | 1199-1204 | 6 | |
| α-helix | 1209-1210 | 2 | |
| α-helix | 1211-1215 | 5 | |
| α-helix | 1216-1221 | 6 | |
| β-strand | 1224-1228 | 5 | 201 |
| β-strand | 1236-1242 | 7 | 201 |
| α-helix | 1250-1254 | 5 | |
| α-helix | 1256-1269 | 14 | |
| β-strand | 1272-1274 | 3 | 200 |
| β-strand | 1281-1292 | 12 | 198 |
| β-strand | 1298-1309 | 12 | 198 |
| α-helix | 1313-1316 | 4 | |
| β-strand | 1322 | 1 | 199 |
| β-strand | 1328-1329 | 2 | 198 |
| α-helix | 1332-1338 | 7 | |
| α-helix | 1341-1357 | 17 | |
| α-helix | 1365-1375 | 11 | |
| α-helix | 1386-1389 | 4 | |
| α-helix | 1396-1400 | 5 | |
| α-helix | 1405-1415 | 11 | |
| β-strand | 1418-1419 | 2 | 176 |
| α-helix | 1424-1429 | 6 | |
| β-strand | 1437 | 1 | 202 |
| β-strand | 1441-1445 | 5 | 12 |
| α-helix | 1447-1452 | 6 | |
Chains Ab and Bb: 2 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 29-50 | 22 | |
| α-helix | 67-103 | 37 | |
Chains AB and BB: 52 helices, 72 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 27-40 | 14 | |
| α-helix | 45-53 | 9 | |
| α-helix | 54-58 | 5 | |
| α-helix | 59-65 | 7 | |
| β-strand | 69-73 | 5 | 205 |
| α-helix | 80-82 | 3 | |
| β-strand | 84-97 | 14 | 205 |
| α-helix | 98-100 | 3 | |
| β-strand | 101-103 | 3 | 206 |
| β-strand | 109-111 | 3 | 206 |
| α-helix | 114-120 | 7 | |
| β-strand | 125-140 | 16 | 205 |
| β-strand | 150-156 | 7 | 205 |
| α-helix | 161-163 | 3 | |
| β-strand | 164-171 | 8 | 205 |
| β-strand | 173 | 1 | 207 |
| α-helix | 180-183 | 4 | |
| α-helix | 186-191 | 6 | |
| β-strand | 203-205 | 3 | 207 |
| β-strand | 208-212 | 5 | 207 |
| β-strand | 214-218 | 5 | 208 |
| β-strand | 224-227 | 4 | 209 |
| β-strand | 234-241 | 8 | 209 |
| β-strand | 253-259 | 7 | 209 |
| β-strand | 268-272 | 5 | 209 |
| β-strand | 280-281 | 2 | 209 |
| α-helix | 282-289 | 8 | |
| α-helix | 294-301 | 8 | |
| α-helix | 309-321 | 13 | |
| α-helix | 327-336 | 10 | |
| α-helix | 345-359 | 15 | |
| α-helix | 371-389 | 19 | |
| α-helix | 395-397 | 3 | |
| α-helix | 401-403 | 3 | |
| β-strand | 404-407 | 4 | 208 |
| α-helix | 409-437 | 29 | |
| α-helix | 441-443 | 3 | |
| α-helix | 444-447 | 4 | |
| α-helix | 451-463 | 13 | |
| β-strand | 466 | 1 | 210 |
| β-strand | 476 | 1 | 210 |
| β-strand | 480-482 | 3 | 207 |
| α-helix | 483-484 | 2 | |
| α-helix | 488-494 | 7 | |
| β-strand | 497-499 | 3 | 208 |
| α-helix | 516-518 | 3 | |
| β-strand | 522 | 1 | 211 |
| β-strand | 536-538 | 3 | 208 |
| β-strand | 539 | 1 | 211 |
| β-strand | 544-545 | 2 | 212 |
| α-helix | 552-560 | 9 | |
| α-helix | 563 | 1 | |
| β-strand | 564-565 | 2 | 213 |
| α-helix | 566-568 | 3 | |
| α-helix | 577 | 1 | |
| β-strand | 578-582 | 5 | 213 |
| β-strand | 585-590 | 6 | 213 |
| α-helix | 593-605 | 13 | |
| β-strand | 614-618 | 5 | 213 |
| β-strand | 623-627 | 5 | 213 |
| β-strand | 633-638 | 6 | 212 |
| β-strand | 640-643 | 4 | 214 |
| β-strand | 648-651 | 4 | 214 |
| α-helix | 655-667 | 13 | |
| α-helix | 681-686 | 6 | |
| β-strand | 690-694 | 5 | 212 |
| α-helix | 697-700 | 4 | |
| β-strand | 703-704 | 2 | 215 |
| α-helix | 707-710 | 4 | |
| β-strand | 740-741 | 2 | 215 |
| α-helix | 745-748 | 4 | |
| α-helix | 759-761 | 3 | |
| α-helix | 764-773 | 10 | |
| β-strand | 792-796 | 5 | 53 |
| β-strand | 800 | 1 | 216 |
| β-strand | 804-805 | 2 | 217 |
| α-helix | 809-812 | 4 | |
| β-strand | 821-827 | 7 | 218 |
| β-strand | 838-842 | 5 | 218 |
| α-helix | 843-847 | 5 | |
| β-strand | 853-862 | 10 | 53 |
| β-strand | 873-874 | 2 | 219 |
| β-strand | 882-883 | 2 | 219 |
| α-helix | 890-892 | 3 | |
| β-strand | 898 | 1 | 220 |
| β-strand | 904-905 | 2 | 53 |
| β-strand | 910 | 1 | 221 |
| β-strand | 912 | 1 | 220 |
| β-strand | 914-917 | 4 | 219 |
| β-strand | 934-936 | 3 | 219 |
| β-strand | 940 | 1 | 221 |
| α-helix | 941-942 | 2 | |
| β-strand | 947-956 | 10 | 53 |
| β-strand | 962-972 | 11 | 53 |
| β-strand | 980-982 | 3 | 218 |
| β-strand | 987-994 | 8 | 218 |
| β-strand | 1001-1002 | 2 | 222 |
| β-strand | 1010-1012 | 3 | 218 |
| α-helix | 1014-1016 | 3 | |
| α-helix | 1023-1038 | 16 | |
| α-helix | 1041 | 1 | |
| β-strand | 1042-1043 | 2 | 217 |
| α-helix | 1052-1060 | 9 | |
| β-strand | 1069-1070 | 2 | 218 |
| β-strand | 1072-1073 | 2 | 222 |
| β-strand | 1080 | 1 | 222 |
| β-strand | 1085-1094 | 10 | 218 |
| α-helix | 1099-1101 | 3 | |
| β-strand | 1104-1106 | 3 | 186 |
| α-helix | 1118-1119 | 2 | |
| β-strand | 1128-1130 | 3 | 185 |
| α-helix | 1132-1141 | 10 | |
| β-strand | 1143 | 1 | 202 |
| α-helix | 1144-1148 | 5 | |
| α-helix | 1149-1153 | 5 | |
| β-strand | 1158-1163 | 6 | 175 |
| β-strand | 1172-1174 | 3 | 223 |
| β-strand | 1179-1181 | 3 | 223 |
| β-strand | 1182 | 1 | 224 |
| β-strand | 1187 | 1 | 224 |
| β-strand | 1191-1196 | 6 | 175 |
| α-helix | 1198-1208 | 11 | |
| β-strand | 1215-1218 | 4 | 176 |
| α-helix | 1219-1222 | 4 | |
Chain Ac: 6 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 11-18 | 8 | |
| α-helix | 21-23 | 3 | |
| α-helix | 24-55 | 32 | |
| α-helix | 64-94 | 31 | |
| α-helix | 96-99 | 4 | |
| α-helix | 101-109 | 9 | |
Chains AC and BC: 12 helices, 12 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 7-13 | 7 | 226 |
| β-strand | 17-23 | 7 | 226 |
| α-helix | 27-39 | 13 | |
| β-strand | 43-54 | 12 | 227 |
| α-helix | 60-67 | 8 | |
| β-strand | 72 | 1 | 228 |
| α-helix | 77-79 | 3 | |
| β-strand | 97-104 | 8 | 227 |
| β-strand | 111-114 | 4 | 229 |
| β-strand | 119-120 | 2 | 227 |
| α-helix | 131 | 1 | |
| β-strand | 132 | 1 | 228 |
| α-helix | 133 | 1 | |
| β-strand | 143-147 | 5 | 229 |
| β-strand | 152-162 | 11 | 227 |
| α-helix | 168-170 | 3 | |
| β-strand | 173-179 | 7 | 226 |
| α-helix | 197-200 | 4 | |
| α-helix | 202-204 | 3 | |
| α-helix | 205-209 | 5 | |
| α-helix | 211-214 | 4 | |
| α-helix | 218-219 | 2 | |
| β-strand | 228-234 | 7 | 226 |
| α-helix | 240-270 | 31 | |
Chains Ad and Bd: 8 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 32-39 | 8 | |
| α-helix | 41-61 | 21 | |
| α-helix | 67-88 | 22 | |
| α-helix | 92-127 | 36 | |
| α-helix | 132-147 | 16 | |
| α-helix | 150-164 | 15 | |
| α-helix | 185-190 | 6 | |
| α-helix | 192-198 | 7 | |
Chains AD and BD: 10 helices, 3 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 36-38 | 3 | 1 |
| β-strand | 44-46 | 3 | 1 |
| β-strand | 49 | 1 | 2 |
| α-helix | 52-77 | 26 | |
| α-helix | 111-114 | 4 | |
| α-helix | 119-133 | 15 | |
| α-helix | 139-150 | 12 | |
| α-helix | 157-168 | 12 | |
| α-helix | 174-182 | 9 | |
| α-helix | 188-194 | 7 | |
| α-helix | 196-198 | 3 | |
| α-helix | 204-217 | 14 | |
| α-helix | 219-220 | 2 | |
34 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| DNA (38-mer) | A | DNA | 38 | Saccharomyces cerevisiae S288C | |
| DNA (37-mer) | B | DNA | 37 | Saccharomyces cerevisiae S288C | |
| Mediator of RNA polymerase II transcription subunit 15 | Ao, Bo | protein | 1081 | Saccharomyces cerevisiae S288C | P19659 (AlphaFold model) |
| Mediator of RNA polymerase II transcription subunit 2 | Ab, Bb | protein | 431 | Saccharomyces cerevisiae S288C | Q12124 (AlphaFold model) |
| Mediator of RNA polymerase II transcription subunit 3 | Ac, Bc | protein | 397 | Saccharomyces cerevisiae S288C | P40356 (AlphaFold model) |
| Mediator of RNA polymerase II transcription subunit 14 | An, Bn | protein | 1082 | Saccharomyces cerevisiae S288C | P19263 (AlphaFold model) |
| Mediator of RNA polymerase II transcription subunit 5 | Ae, Be | protein | 1132 | Saccharomyces cerevisiae S288C | P53114 |
| Mediator of RNA polymerase II transcription subunit 16 | Ap, Bp | protein | 974 | Saccharomyces cerevisiae S288C | P32259 |
| Regulatory protein GAL4 | GA, GB | protein | 147 | Saccharomyces cerevisiae S288C | P04386 |
| Mediator of RNA polymerase II transcription subunit 20 | At, Bt | protein | 210 | Saccharomyces cerevisiae S288C | P34162 |
| Mediator of RNA polymerase II transcription subunit 7 | Ag, Bg | protein | 222 | Saccharomyces cerevisiae S288C | Q08278 |
| Mediator of RNA polymerase II transcription subunit 8 | Ah, Bh | protein | 223 | Saccharomyces cerevisiae S288C | P38304 |
29 more molecules are not listed.
Sequence of entity 1 (A), FASTA
>7UIO_1 DNA (38-MER) (chains A)
ACCGGAGGACAGTCCTCCCGACTGACTGACGTCGTACG
Sequence of entity 2 (B), FASTA
>7UIO_2 DNA (37-MER) (chains B)
CGTACGACGTCAGTCAGTCGGGAGGACTGTCCTCCGG
Sequence of entity 3 (Ao, Bo), FASTA
>7UIO_3 Mediator of RNA polymerase II transcription subunit 15 (chains Ao, Bo)
MSAAPVQDKDTLSNAERAKNVNGLLQVLMDINTLNGGSSDTADKIRIHAKNFEAALFAKS
SSKKEYMDSMNEKVAVMRNTYNTRKNAVTAAAANNNIKPVEQHHINNLKNSGNSANNMNV
NMNLNPQMFLNQQAQARQQVAQQLRNQQQQQQQQQQQQRRQLTPQQQQLVNQMKVAPIPK
QLLQRIPNIPPNINTWQQVTALAQQKLLTPQDMEAAKEVYKIHQQLLFKARLQQQQAQAQ
AQANNNNNGLPQNGNINNNINIPQQQQMQPPNSSANNNPLQQQSSQNTVPNVLNQINQIF
SPEEQRSLLQEAIETCKNFEKTQLGSTMTEPVKQSFIRKYINQKALRKIQALRDVKNNNN
ANNNGSNLQRAQNVPMNIIQQQQQQNTNNNDTIATSATPNAAAFSQQQNASSKLYQMQQQ
QQAQAQAQAQAQAQAQAQAQAQAAQAAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQ
AHAQHQPSQQPQQAQQQPNPLHGLTPTAKDVEVIKQLSLDASKTNLRLTDVTNSLSNEEK
EKIKMKLKQGQKLFVQVSNFAPQVYIITKNENFLKEVFQLRIFVKEILEKCAEGIFVVKL
DTVDRLIIKYQKYWESMRIQILRRQAILRQQQQMANNNGNPGTTSTGNNNNIATQQNMQQ
SLQQMQHLQQLKMQQQQQQQQQQQQQQQQQQQQQQQHIYPSSTPGVANYSAMANAPGNNI
PYMNHKNTSSMDFLNSMENTPKVPVSAAATPSLNKTINGKVNGRTKSNTIPVTSIPSTNK
KLSISNAASQQPTPRSASNTAKSTPNTNPSPLKTQTKNGTPNPNNMKTVQSPMGAQPSYN
SAIIENAFRKEELLLKDLEIRKLEISSRFKHRQEIFKDSPMDLFMSTLGDCLGIKDEEML
TSCTIPKAVVDHINGSGKRKPTKAAQRARDQDSIDISIKDNKLVMKSKFNKSNRSYSIAL
SNVAAIFKGIGGNFKDLSTLVHSSSPSTSSNMDVGNPRKRKASVLEISPQDSIASVLSPD
SNIMSDSKKIKVDSPDDPFMTKSGATTSEKQEVTNEAPFLTSGTSSEQFNVWDWNNWTSA
T
Sequence of entity 4 (Ab, Bb), FASTA
>7UIO_4 Mediator of RNA polymerase II transcription subunit 2 (chains Ab, Bb)
MVVQNSPVSSVHTANFSERGSNTRTMTYKNKLTVCFDDILKVGAEMMMQQQLKNVQLDSY
LVNGFSQSQQKLLKEKVKLFHGILDDLETSLSQSSSYLETLTALGKEKEKEREEAEKKRA
EQENMRKVREQEELKKRQELEEASQQQQLQQNSKEKNGLGLNFSTTAPANTTDANGSKEN
YQELGSLQSSSQTQLENANAANNGAAFSPLTTTRIQSQQAQPSDVMFNDLNSMDISMFSG
LDSTGFDSTAFNATVDETKGFDDNDSGNNYNDINISSIENNINNNINSTKNGKDNNNESN
KNNNGDEKNKNNNEDNENNNNSSEKNNNNNNNNNNNNDDNGNNNNNNSGNDNNNTTNNDS
NNKNNSITTGNDNENIVNNDLPTTVVSNPGDNPPPADNGEEYLTLNDFNDLNIDWSTTGD
NGELDLSGFNI
Sequence of entity 5 (Ac, Bc), FASTA
>7UIO_5 Mediator of RNA polymerase II transcription subunit 3 (chains Ac, Bc)
MDSIIPAGVKLDDLQVILAKNENETRDKVCKQINEARDEILPLRLQFNEFIQIMANIDQE
GSKQADRMAKYLHIRDKILQLNDRFQTLSSHLEALQPLFSTVPEYLKTADNRDRSFQLLE
PLSTYNKNGNAVCSTATVVSTNHSAAASTPTTTATPHANPITHAHSLSNPNSTATMQHNP
LAGKRGPKSGSTMGTPTVHNSTAAAPIAAPKKPRKPRQTKKAKAQAQAQAQAQAQVYAQQ
STVQTPITASMAAALPNPTPSMINSVSPTNVMGTPLTNMMSPMGNAYSMGAQNQGGQVSM
SQFNGSGNGSNPNTNTNSNNTPLQSQLNLNNLTPANILNMSMNNDFQQQQQQQQQQQQPQ
PQYNMNMGMNNMNNGGKELDSLDLNNLELGGLNMDFL
Sequence of entity 6 (An, Bn), FASTA
>7UIO_6 Mediator of RNA polymerase II transcription subunit 14 (chains An, Bn)
MTTTIGSPQMLANEERLSNEMHALKNRSEQNGQEQQGPVKNTQLHGPSATDPETTATQKE
SLEMVPKDTSAATMTSAPPPALPHVEINQVSLALVIRNLTVFTMKELAQYMKTNVHTQAN
EPNSAKKIRFLQLIIFLRTQFLKLYVLVKWTRTIKQNNFHVLIDLLNWFRTTNMNVNNCI
WALKSSLNSMTNAKLPNVDLVTALEVLSLGRPNLPTHNFKLSGVSNSMDMVDGMAKVPIG
LILQRLKDLNLTVSIKIALMNIPKPLNSYHIKNGRIYFTVPNEFEIQLSTVNRQSPLFFV
DLKLLFNTEAEQTVSAVTEATSTNGDSENNEENSSSNGNNLPLNKPRLEKLINEILLKSN
DPLLSLYNFLHKYVLTLQLYMVHREFLKLANGGKFSKSNLIHNYDSKKSTITVRYWLNGK
MDSKGKITIGIQRTTESLILKWDNQSASRAKNMPVIYNNIVSNIEGILDEIMFNHARIIR
SELLARDIFQEDEENSDVLLFQLPTTCVSMAPIQLKIDLLSGQFYFRNPTPLLSNYASKI
NRAEGPEELARILQQLKLDKIIHVLTTMFENTGWSCSRIIKIDKPIRTQVNTGGESVVKK
EDNKYAIAGNSTTNSDVSLLLQRDLFIRLPHWPLNWYLILSIISSKTSCVVEKRIGKIVS
QRGKWNLKYLDNSNVMTVKLESITYQKIMILQRTILNRIINHMLIDSLNQLEIRNKICSS
EMINEQKLPQYIIQGSNTNDNISIITLELESFLEGSKALNSILESSMFLRIDYSNSQIRL
YAKFKRNTMMIQCQIDKLYIHFVQEEPLAFYLEESFTNLGIIVQYLTKFRQKLMQLVVLT
DVVERLHKNFESENFKIIALQPNEISFKYLSNNDEDDKDCTIKISTNDDSIKNLTVQLSP
SNPQHIIQPFLDNSKMDYHFIFSYLQFTSSLFKALKVILNERGGKFHESGSQYSTMVNIG
LHNLNEYQIVYYNPQAGTKITICIELKTVLHNGRDKIQFHIHFADVAHITTKSPAYPMMH
QVRNQVFMLDTKRLGTPESVKPANASHAIRLGNGVACDPSEIEPILMEIHNILKVDSNSS
SS
Sequence of entity 7 (Ae, Be), FASTA
>7UIO_7 Mediator of RNA polymerase II transcription subunit 5 (chains Ae, Be)
MEKESVYNLALKCAERQLTSMEFSNLYKEFFNEKFPSLIQEEEEDTTTTANINEVKKASD
LVDTPSNNTAATADTTHLHEALDIVCSDFVKILNLEKPLILADYIVEVLLVNYNSDMIKC
FLPKLNSVRNSLLLAHFFSKSCSFFAKLSDTLIIDQVRKDLGNVIVPNILSLDMNSMNKE
LIAIVSKLLQTTLKLSPSPILLTSAGCKNGSFTLLNQLSQTNKLLFKRVSQTFEAKLHFK
DTKPFLNKDSTNEFVGSPSLTSPQYIPSPLSSTKPPGSVNSAAKYKDMKLLRYYKNIWLN
NKIINWEISNPDFLSKYSAITSSIFQESFNSVQNLDQLLTDLIETSFTCFAQFVSNKQYH
QANSNLTLLERKWVIFITKHLPLLILENSSRSPRVVTNALDNIDEKVVKAIRIYFTEKDD
NKTNNEDLFDDYPSTSLDIRHDFIKGLIMLNLQPASVINNYLREDQMIDTSILPTRDDLF
VRNLQGIQEVVHNTNSFIISSLDTLELESITESITHDSSNGLFQVLHNFESVAPTKQREI
VKAFLSIFEDAIKELNYNRIAKICALLFFNFSHSLTTILSFSSPAALMKTLIKFVDLSRN
GRNGSNGNDESSEYETINISLSFSWAILLIINLTQTYGISVVDVALKYPELSIKNSFIIN
FISNLPNVSDKYYLEESNVNDSDMLTKSHNTVQSWLCDLFVNGSITDQLIQNIETRQLAN
LIPFIVKQVLLSVEIGVLTDISSLIGGFEYFLQPLLLVGLIKTFYWLEQFLSCVKNDTIS
EDILQGIFNLLNTLFNPVTLNEDSKAFHTAVLRLNAIPLLKVLRKFRVQSQSNYGIYSSD
AQGDPNLEPLIAKLVAVLNVSPVYDVDPRIINSENDYSRKQLGYGKFLILNENPINKIMT
NQINSFWSLHSSTYYNLDYLFELIELVTPKSFLFDVLKTLEYKLATYGVPGSENKRGSLD
SEHVFDYFFYFLVLYDVKTAEEASQLIEYMENDAKKSKGDVDIKGEDLHEKNDSAEVRQE
TQPKAEATQDDDFDMLFGENDTSTQAYEEEEENEDNDGNNRTNNVPMIKAEETPSKTNKI
SILKRHSFAVLLHERKLLNDLALENGEITKTENEKFISYHDKYLCMLKTCVF
Sequence of entity 8 (Ap, Bp), FASTA
>7UIO_8 Mediator of RNA polymerase II transcription subunit 16 (chains Ap, Bp)
MMLGEHLMSWSKTGIIAYSDSQSSNANICLTFLESINGINWRFHTPQKYVLHPQLHEVQY
QESSSTLSTHSTTTSVNGSTTAGVGSTPNFGGNSNKSPPQFFYNISSIHWNNWFSLPGDM
LAVCDELGNMTMLITGQRPDRATTYEKLTMVFQDNVYKIYNHVMPLKPVDKLKPMNIERK
QTRKEYNTSILEFRWLTSSKSVIVSQFCAFDSSSNTYRSRAQQVPPYGVYHPPFIKYACL
AIRKNGQIDFWYQFSNSKDHKKITLQLLDTSNQRFKDLQWLEFARITPMNDDQCMLITTY
SKLSKNISFYKLHVNWNLNATKPNVLNDPSLKIQFILSTTLDPTDDEGHVLKLENLHVVS
KSSIEKDPSPEILVLYNVCDTSKSLVKRYRLAPTQLSAEYLVILKPDLNIDRNNSTNQIF
QSRRYNLRRHSDIVLDKKVTLITSEMFDAFVSFYFEDGTIESYNQNDWKLETERLISQSQ
LGKFKNIIASPLSAGFNYGKLPLPPSVEWMKVSPSMCGVIVKQYNKKWPQFYAAVQKNYA
DPEKDSINATALAFGYVKSLHKQISAEDLTIAAKTHILRISFLDRKRAKEFITTLLKSLY
SFFNISPDAPKEIMDKIITSRPLQKIMLLQLELGSCFSQENIEEMARVILYLKNVLFAFN
GVARNFHFAIEQISNNSNQQQNPKLFQTIFSKQDLIHSLIPVAKWFVKFITYLTQEILIL
INDPTNKEYTLVHGIFGAKMSRTLILSILNEIKKVTQIVAKFPETSYPILNESSTFLKLV
LSESPVDFEKFETFLVDVNNKFIALCEQQPSQEREFSLLVKAEIPPEYAKVGDFLLQYAN
NAVISHANAAAVYFADTSGLKISNSEFFNPEIFHLLQPLEEGLIIDTDKLPIKNRTSKSF
SKLLYDDVTCDKLSVSEISDGKLKRCSRCGSVTRAGNIISSDKTIVPTSIQTKRWPTMYT
RLCICSGMLFEMDG
Sequence of entity 9 (GA, GB), FASTA
>7UIO_9 Regulatory protein GAL4 (chains GA, GB)
MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKRSPLTRAHLTEVESR
LERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNKDAVTDRLASVETDMPL
TLRQHRISATSSSEESSNKGQRQLTVS
Sequence of entity 10 (At, Bt), FASTA
>7UIO_10 Mediator of RNA polymerase II transcription subunit 20 (chains At, Bt)
MGKSAVIFVERATPATLTELKDALSNSILSVRDPWSIDFRTYRCSIKNLPADVSKLMYSI
TFHHHGRQTVLIKDNSAMVTTAAAADIPPALVFNGSSTGVPESIDTILSSKLSNIWMQRQ
LIKGDAGETLILDGLTVRLVNLFSSTGFKGLLIELQADEAGEFETKIAGIEGHLAEIRAK
EYKTSSDSLGPDTSNEICDLAYQYVRALEL
Sequence of entity 11 (Ag, Bg), FASTA
>7UIO_11 Mediator of RNA polymerase II transcription subunit 7 (chains Ag, Bg)
MSNDPGNEVSSLYPPPPPYVKFFTQSNLEKLPKYKEKKAASAKQTAPNNSNGGSEEEITC
ALDYLIPPPMPKNQQYRAFGSIWQVKDQLPDLESMGLTQLYKKSTENESTNYQYKIQELR
KLLKSLLLNYLELIGVLSINPDMYERKVENIRTILVNIHHLLNEYRPHQSRESLIMLLEE
QLEYKRGEIREIEQVCKQVHDKLTSIQDTLRTGSQSPPSSSQ
Sequence of entity 12 (Ah, Bh), FASTA
>7UIO_12 Mediator of RNA polymerase II transcription subunit 8 (chains Ah, Bh)
MSQSTASLVPEGNQGSLQEDVSFDFNGVPGQALDAVRMRLAQLTHSLRRIRDEMSKAELP
QWYTLQSQLNVTLSQLVSVTSTLQHFQETLDSTVVYPLPKFPTTSHESLVTTLLRKKNIP
EVDEWMKYVRETSGVTTALLKDEEIEKLLQQDREITNWARTTFRNEYGKHDFKNEESLSE
EHASLLVRDSKPSKPFNVDDVLKFTFTGEKPIITGSTSTSSSN
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| ASP | Aspartic acid | C4 H7 N O4 | 1 |
| ALA | Alanine | C3 H7 N O2 | 1 |
| THR | Threonine | C4 H9 N O3 | 1 |
| ZN | Zinc ion | Zn | 4 |
Primary citation
Structural basis of a transcription pre-initiation complex on a divergent promoter. Gorbea Colon, J.J., Palao 3rd, L., Chen, S.F. et al. Mol Cell (2023) 83:574. DOI 10.1016/j.molcel.2023.01.011 · PubMed
Other PDB entries of the same protein (UniProt P19659 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 7UIC 3.7 Å, Mediator-PIC Early (Tail A)
- 7UIL 4.3 Å, Mediator-PIC Early (Tail A/B Dimer)
- 7UIK 7.7 Å, Mediator-PIC Early (Tail A + Upstream DNA & Activator)
- 2K0N Solution Structure of Yeast Gal11p kix domain
- 2LPB Structure of the complex of the central activation domain of Gcn4 bound to the mediator…
- 6ALY Solution structure of yeast Med15 ABD2 residues 277-368
- 8ZZ3 Molecular architecture of the yeast Mediator complex
Browse structure collections
About this viewer
MolViewer shows 7UIO directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.