7UVL: IgA1 Protease with IgA1 substrate

IgA1 Protease with IgA1 substrate. Determined by electron microscopy at 3.56 Å resolution. Released 23 Nov 2022.

Method: Electron microscopy
Resolution: 3.56 Å
Organisms: Gemella haemolysans, Homo sapiens
Chains: 5
Atoms: 16,508
Mol. weight: 240.95 kDa
Released: 23 Nov 2022

Explore 7UVL in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7UVL contains 78 α-helices and 153 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 11 helices, 17 β-strands

Element	Residues	Length	Sheet
β-strand	245-249	5	22
α-helix	250-252	3
α-helix	253	1
α-helix	254-258	5
β-strand	264-271	8	22
β-strand	278-281	4	23
β-strand	290-291	2	22
β-strand	295-296	2	22
α-helix	298-300	3
β-strand	302-308	7	22
α-helix	312-317	6
α-helix	319-320	2
β-strand	321-327	7	23
β-strand	330-338	9	23
β-strand	348-352	5	24
α-helix	353-355	3
α-helix	356-361	6
β-strand	365-374	10	24
β-strand	379-385	7	25
β-strand	388-389	2	25
α-helix	390-391	2
α-helix	392-394	3
β-strand	395-397	3	24
β-strand	402	1	24
β-strand	411-419	9	24
α-helix	421-426	6
β-strand	430-436	7	25
β-strand	443-448	6	25

Chain B: 11 helices, 17 β-strands

Element	Residues	Length	Sheet
β-strand	245-249	5	18
α-helix	250-252	3
α-helix	253-257	5
β-strand	264-271	8	18
β-strand	278-281	4	19
β-strand	290-291	2	18
β-strand	295-296	2	18
α-helix	298-300	3
β-strand	302-308	7	18
α-helix	312-316	5
α-helix	319-320	2
β-strand	321-327	7	19
β-strand	330-338	9	19
β-strand	348-352	5	20
α-helix	353-355	3
α-helix	356-361	6
β-strand	365-374	10	20
β-strand	379-385	7	21
β-strand	388-389	2	21
α-helix	390-391	2
α-helix	392-394	3
β-strand	395-397	3	20
α-helix	398-400	3
β-strand	401-402	2	20
β-strand	411-419	9	20
α-helix	421-426	6
β-strand	430-436	7	21
β-strand	443-448	6	21

Chain H: 7 helices, 21 β-strands

Element	Residues	Length	Sheet
β-strand	5-7	3	35
β-strand	12	1	36
β-strand	18-23	6	35
β-strand	33-39	7	37
β-strand	45-51	7	37
β-strand	60-62	3	37
β-strand	70	1	35
β-strand	73-75	3	35
β-strand	80-85	6	35
α-helix	90-92	3
β-strand	94-101	8	37
α-helix	108-110	3
β-strand	113-115	3	37
β-strand	117	1	36
α-helix	121-122	2
β-strand	126-128	3	38
α-helix	135-137	3
β-strand	140-150	11	38
β-strand	158-159	2	39
β-strand	170-171	2	38
β-strand	175-176	2	38
β-strand	183-192	10	38
α-helix	193-197	5
β-strand	202-204	3	40
β-strand	205-206	2	39
β-strand	216-218	3	40
α-helix	223-225	3
α-helix	228-231	4

Chain L: 5 helices, 23 β-strands

Element	Residues	Length	Sheet
β-strand	4-6	3	26
β-strand	10-13	4	27
β-strand	19-21	3	28
β-strand	23-25	3	26
β-strand	38-40	3	29
β-strand	41-42	2	30
β-strand	50-51	2	30
α-helix	59-60	2
β-strand	67	1	28
β-strand	78-80	3	28
β-strand	93-95	3	29
β-strand	103	1	29
β-strand	108-111	4	27
α-helix	117-119	3
β-strand	120-123	4	31
α-helix	124-126	3
α-helix	127-131	5
β-strand	134-144	11	31
β-strand	149-152	4	32
β-strand	153-154	2	33
β-strand	155	1	34
β-strand	159-160	2	33
β-strand	165-168	4	31
β-strand	178-187	10	31
α-helix	188-193	6
β-strand	196-198	3	34
β-strand	200-203	4	32
β-strand	213-215	3	34

Chain P: 44 helices, 75 β-strands

Element	Residues	Length	Sheet
β-strand	923	1	1
β-strand	928-931	4	2
β-strand	934-935	2	2
β-strand	942-943	2	2
β-strand	957-963	7	2
β-strand	969-972	4	2
β-strand	973-979	7	3
β-strand	986-991	6	3
β-strand	996	1	1
β-strand	998-1000	3	4
β-strand	1003-1005	3	4
β-strand	1009-1012	4	3
α-helix	1026-1035	10
β-strand	1040-1042	3	5
β-strand	1047-1048	2	5
α-helix	1053-1055	3
β-strand	1062	1	6
β-strand	1070-1072	3	5
β-strand	1078	1	7
β-strand	1081-1082	2	5
β-strand	1084	1	8
β-strand	1089	1	6
β-strand	1092-1093	2	6
β-strand	1096-1098	3	5
β-strand	1101-1102	2	5
β-strand	1105	1	8
β-strand	1108-1109	2	5
β-strand	1112-1114	3	9
β-strand	1117-1122	6	6
β-strand	1125-1127	3	5
β-strand	1130-1137	8	5
β-strand	1140-1142	3	9
β-strand	1145-1148	4	6
β-strand	1152-1164	13	5
β-strand	1173-1175	3	9
β-strand	1178-1182	5	6
β-strand	1187-1199	13	5
β-strand	1207-1209	3	9
β-strand	1212-1216	5	6
β-strand	1220-1222	3	5
β-strand	1225-1226	2	5
β-strand	1230-1232	3	5
β-strand	1238-1240	3	9
β-strand	1243-1245	3	6
β-strand	1259-1261	3	5
β-strand	1263-1265	3	5
β-strand	1270	1	5
β-strand	1273	1	9
β-strand	1276	1	6
β-strand	1288-1290	3	5
α-helix	1327-1329	3
α-helix	1333-1335	3
β-strand	1337	1	7
α-helix	1363-1372	10
α-helix	1378-1386	9
α-helix	1393-1395	3
β-strand	1398-1402	5	10
α-helix	1413-1416	4
β-strand	1425-1428	4	10
β-strand	1433-1434	2	10
β-strand	1439-1440	2	11
β-strand	1450-1452	3	11
β-strand	1457-1459	3	11
α-helix	1469-1480	12
α-helix	1487-1492	6
α-helix	1507-1515	9
α-helix	1517-1528	12
β-strand	1530-1531	2	2
α-helix	1538-1549	12
α-helix	1551-1564	14
β-strand	1568-1569	2	12
β-strand	1572-1573	2	12
α-helix	1574-1579	6
α-helix	1582-1584	3
α-helix	1592-1600	9
α-helix	1604-1607	4
α-helix	1609-1611	3
α-helix	1612-1615	4
α-helix	1616-1620	5
α-helix	1629-1639	11
α-helix	1646-1653	8
β-strand	1657-1659	3	13
α-helix	1666-1673	8
α-helix	1683-1688	6
α-helix	1695-1698	4
β-strand	1710-1714	5	13
β-strand	1718-1723	6	13
α-helix	1724-1727	4
α-helix	1736-1763	28
α-helix	1769-1773	5
β-strand	1776-1779	4	13
β-strand	1784	1	14
β-strand	1793	1	14
α-helix	1802-1803	2
α-helix	1804-1808	5
β-strand	1823-1824	2	13
β-strand	1829-1830	2	13
α-helix	1839-1852	14
β-strand	1888	1	15
α-helix	1911-1914	4
α-helix	1918-1944	27
α-helix	1948-1954	7
β-strand	1955-1961	7	16
α-helix	1963-1965	3
β-strand	1974-1978	5	16
α-helix	1982-1987	6
α-helix	1991-1997	7
β-strand	1999-2001	3	16
β-strand	2010-2011	2	16
β-strand	2020	1	15
β-strand	2025	1	16
α-helix	2039-2051	13
α-helix	2067-2073	7
β-strand	2077-2079	3	17
β-strand	2082-2084	3	17
α-helix	2089-2096	8
α-helix	2104-2121	18
α-helix	2145-2159	15
α-helix	2174-2189	16

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
LPXTG-motif cell wall anchor domain protein	P	protein	1295	Gemella haemolysans	C5NYF3
Immunoglobulin alpha-1 heavy constant	A, B	protein	209	Homo sapiens	P01876 (AlphaFold model)
Immunoglobulin alpha-1 light chain	L	protein	219	Homo sapiens
Immunoglobulin alpha-1 heavy chain	H	protein	232	Homo sapiens

Sequence of entity 1 (P), FASTA

>7UVL_1 LPXTG-motif cell wall anchor domain protein (chains P)
DEKKDDIKEEKQVDKKLELRNISNVELYTLENNKYRHVSSLSSVPTNPEAYFMKVKSENF
KDVMLPVKSIESARKDNQDVYKIVGQANDLIQHENNITLENYTYYLPKTVNSENGVYTSF
KNLVDAMNINPYGTFRLGATMDAREVELSDGQESYINKEFSGKLIGENKGKYYAIYNLKK
PLFKALSHATIQDLSIKEANVSSKEDAATIAKEAKNDTTIANVHSSGVIAGERSIGGLIS
QVTDSTISNSSFTGRITNTYDTTATYQIGGLVGKLSGVGALIEKSISSIDMATNANTGDQ
VVGGVAGVVDKKATIRNSYVEGNLNNVKPFGKVGGVVGNLWDRETSEVSNSGNLTNVLSD
VNVTNGNAIAGYDFNGIKATNTYSNKNNKVVKVVQVDDEVLSKDSEEQRGTVLENNIVLE
KKIELVPKKNTKIEDFNFSSRYETDYKNLKDADVSRLRVYKNIEKLLPFYNRETIVKYGN
LVDANNTLYTKDLVSVVPMKDKEVISDINKNKTSINKLLLHYSDNTSQTLDIKYLQDFSK
VAEYEIANTKLIYTPNTLLHSYNNIVKAVLNDLKSVQYDSDAVRKVLDISSNIKLTELYL
DEQFTKTKANIEDSLSKLLSADAVIAENSNSIIDNYVIEKIKNNKEALLLGLTYLERWYN
FKYDNTSAKDLVLYHLDFFGKSNSSALDNVIELGKSGFNNLLAKNNVITYNVLLSKNYGT
EGLFKALEGYRKVFLPNVSNNDWFKTQSKAYIVEEKSTIPEVSSKQSKQGTEHSIGVYDR
LTSPSWKYQSMVLPLLTLPEEKMIFMIANISTIGFGAYDRYRSSEYPKGDKLNRFVEENA
QAAAKRFRDHYDYWYKILDKENKEKLFRSVLVYDAFRFGNDTNKETQEANFETNNPVIKN
FFGPAGNNVVHNKHGAYATGDAFYYMAYRMLDKSGAVTYTHAMTHNSDREIYLGGYGRRS
GLGPEFYAKGLLQAPDHSYDPTITINSVLKYDDSENSTRLQIADPTQRFTNVEDLHNYMH
NMFDLIYTLEILEGRAVAKLDYNEKNDLLRKIENIYKKDPDGNSVYATNAVRRLTSDEIK
NLTSFDKLIENDVITRRGYIDQGEYERNGYHTINLFSPIYSALSSKIGTPGDLMGRRMAF
ELLAAKGYKEGMVPYISNQYEKEAKDRGSKIRSYGKEIGLVTDDLVLEKVFNKKYGSWVE
FKKDMYKERVEQFSKLNRVSFFDPNGPWGRQKNVTVNNISVLEKMIETAVREDAEDFTAQ
VYPDTNSRVLKLKKAIFKAYLDQTKDFRTSIFGGK

Sequence of entity 2 (A, B), FASTA

>7UVL_2 Immunoglobulin alpha-1 heavy constant (chains A, B)
CHPRLSLHRPALEDLLLGSEANLTCTLTGLRDASGVTFTWTPSSGKSAVQGPPERDLCGC
YSVSSVLPGCAEPWNHGKTFTCTAAYPESKTPLTATLSKSGNTFRPEVHLLPPPSEELAL
NELVTLTCLARGFSPKDVLVRWLQGSQELPREKYLTWASRQEPSQGTTTFAVTSILRVAA
EDWKKGDTFSCMVGHEALPLAFTQKTIDR

Sequence of entity 3 (L), FASTA

>7UVL_3 Immunoglobulin alpha-1 light chain (chains L)
DIVMTQSPLSLSVTPGEPASISCRSSQSLLRRDGHNDLEWYLQKPGQSPQPLIYLGSTRA
SGVPDRFSGSGSGTDFTLKIIRVEAEDAGTYYCMQNKQTPLTFGQGTRLEIKRTVAAPSV
FIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSL
SSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC

Sequence of entity 4 (H), FASTA

>7UVL_4 Immunoglobulin alpha-1 heavy chain (chains H)
EVQLVESGGGLVQPGGSLKLSCAASGFTLSGSNVHWVRQASGKGLEWVGRIKRNAESDAT
AYAASMRGRLTISRDDSKNTAFLQMNSLKSDDTAMYYCVIRGDVYNRQWGQGTLVTVSSA
SPTSPKVFPLSLCSTQPDGNVVIACLVQGFFPQEPLSVTWSESGQGVTARNFPPSQDASG
DLYTTSSQLTLPATQCLAGKSVTCHVKHYTNPSQDVTVPCPVPSTPPTPSPS

Primary citation

A substrate-induced gating mechanism is conserved among Gram-positive IgA1 metalloproteases. Redzic, J.S., Rahkola, J., Tran, N. et al. Commun Biol (2022) 5:1190-1190. DOI 10.1038/s42003-022-04173-3 · PubMed

Other PDB entries of the same protein (UniProt C5NYF3), best resolution first:

9ECT 1.75 Å, Crystal Structure of the Gemella haemolysans Immunoglobulin A1 Protease Trypsin-Like…
7UVK 3.28 Å, G. haemolysans IgA1 protease

Browse structure collections

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