7V99: Catalytic core of human telomerase holoenzyme

catalytic core of human telomerase holoenzyme. Determined by electron microscopy at 3.54 Å resolution. Released 30 Mar 2022.

Method: Electron microscopy
Resolution: 3.54 Å
Organism: Homo sapiens
Chains: 5
Atoms: 14,582
Mol. weight: 307.84 kDa
Released: 30 Mar 2022

Explore 7V99 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7V99 contains 50 α-helices and 34 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 41 helices, 30 β-strands

Element	Residues	Length	Sheet
α-helix	5-17	13
β-strand	21-23	3	1
α-helix	24-28	5
α-helix	32-41	10
α-helix	45-52	8
β-strand	54-57	4	1
α-helix	77-91	15
β-strand	95	1	2
β-strand	102-103	2	3
β-strand	106-108	3	4
β-strand	113-115	3	4
β-strand	118-119	2	3
α-helix	123-130	8
α-helix	133-139	7
α-helix	144-153	10
β-strand	157-162	6	1
β-strand	168-170	3	1
β-strand	174	1	2
α-helix	176-179	4
α-helix	323-324	2
β-strand	325-326	2	5
α-helix	346-349	4
α-helix	354-358	5
α-helix	385-388	4
α-helix	391-401	11
α-helix	405-412	8
α-helix	416-429	14
α-helix	437-453	17
α-helix	458-470	13
α-helix	475-478	4
α-helix	481-495	15
α-helix	502	1
β-strand	503-504	2	5
α-helix	505-508	4
α-helix	531-557	27
β-strand	561-564	4	6
β-strand	574-577	4	6
α-helix	578-595	18
β-strand	598-600	3	7
α-helix	603-610	8
β-strand	617-625	9	7
β-strand	630-637	8	7
α-helix	645-671	27
α-helix	683-698	16
α-helix	703-705	3
β-strand	707-713	7	8
α-helix	722-736	15
β-strand	740-750	11	1
β-strand	755-765	11	1
α-helix	773-782	10
β-strand	789-799	11	1
α-helix	802-814	13
β-strand	816-819	4	7
β-strand	824-827	4	7
α-helix	836-853	18
α-helix	857-860	4
β-strand	862-865	4	8
β-strand	870-874	5	8
α-helix	877-888	12
β-strand	900-906	7	8
β-strand	920	1	8
β-strand	928-929	2	9
β-strand	933-935	3	9
β-strand	942-944	3	9
α-helix	954-957	4
α-helix	966-981	16
α-helix	994-1017	24
α-helix	1030-1050	21
α-helix	1067-1082	16
α-helix	1091-1106	16
α-helix	1109-1118	10

Chain K: 5 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	17-21	5
α-helix	28-36	9
β-strand	42-43	2	10
α-helix	47-71	25
β-strand	77-78	2	11
α-helix	80-89	10
α-helix	91-97	7

Chain L: 4 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	38-48	11
β-strand	53-54	2	11
α-helix	56-82	27
β-strand	88-89	2	10
α-helix	91-101	11
α-helix	104-123	20

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Telomerase reverse transcriptase	A	protein	1132	Homo sapiens	O14746 (AlphaFold model)
Histone H2A type 1-B/E	K	protein	129	Homo sapiens	P04908 (AlphaFold model)
Histone H2B type 1-K	L	protein	125	Homo sapiens	O60814 (AlphaFold model)
Telomerase RNA component	R	RNA	451	Homo sapiens
Primer DNA	S	DNA	24	Homo sapiens

Sequence of entity 1 (A), FASTA

>7V99_1 Telomerase reverse transcriptase (chains A)
MPRAPRCRAVRSLLRSHYREVLPLATFVRRLGPQGWRLVQRGDPAAFRALVAQCLVCVPW
DARPPPAAPSFRQVSCLKELVARVLQRLCERGAKNVLAFGFALLDGARGGPPEAFTTSVR
SYLPNTVTDALRGSGAWGLLLRRVGDDVLVHLLARCALFVLVAPSCAYQVCGPPLYQLGA
ATQARPPPHASGPRRRLGCERAWNHSVREAGVPLGLPAPGARRRGGSASRSLPLPKRPRR
GAAPEPERTPVGQGSWAHPGRTRGPSDRGFCVVSPARPAEEATSLEGALSGTRHSHPSVG
RQHHAGPPSTSRPPRPWDTPCPPVYAETKHFLYSSGDKEQLRPSFLLSSLRPSLTGARRL
VETIFLGSRPWMPGTPRRLPRLPQRYWQMRPLFLELLGNHAQCPYGVLLKTHCPLRAAVT
PAAGVCAREKPQGSVAAPEEEDTDPRRLVQLLRQHSSPWQVYGFVRACLRRLVPPGLWGS
RHNERRFLRNTKKFISLGKHAKLSLQELTWKMSVRDCAWLRRSPGVGCVPAAEHRLREEI
LAKFLHWLMSVYVVELLRSFFYVTETTFQKNRLFFYRKSVWSKLQSIGIRQHLKRVQLRE
LSEAEVRQHREARPALLTSRLRFIPKPDGLRPIVNMDYVVGARTFRREKRAERLTSRVKA
LFSVLNYERARRPGLLGASVLGLDDIHRAWRTFVLRVRAQDPPPELYFVKVDVTGAYDTI
PQDRLTEVIASIIKPQNTYCVRRYAVVQKAAHGHVRKAFKSHVSTLTDLQPYMRQFVAHL
QETSPLRDAVVIEQSSSLNEASSGLFDVFLRFMCHHAVRIRGKSYVQCQGIPQGSILSTL
LCSLCYGDMENKLFAGIRRDGLLLRLVDDFLLVTPHLTHAKTFLRTLVRGVPEYGCVVNL
RKTVVNFPVEDEALGGTAFVQMPAHGLFPWCGLLLDTRTLEVQSDYSSYARTSIRASLTF
NRGFKAGRNMRRKLFGVLRLKCHSLFLDLQVNSLQTVCTNIYKILLLQAYRFHACVLQLP
FHQQVWKNPTFFLRVISDTASLCYSILKAKNAGMSLGAKGAAGPLPSEAVQWLCHQAFLL
KLTRHRVTYVPLLGSLRTAQTQLSRKLPGTTLTALEAAANPALPSDFKTILD

Sequence of entity 2 (K), FASTA

>7V99_2 Histone H2A type 1-B/E (chains K)
SGRGKQGGKARAKAKTRSSRAGLQFPVGRVHRLLRKGNYSERVGAGAPVYLAAVLEYLTA
EILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGRVTIAQGGVLPNIQAVLLPKKT
ESHHKAKGK

Sequence of entity 3 (L), FASTA

>7V99_3 Histone H2B type 1-K (chains L)
PEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSVYVYKVLKQVHPDTGISSKAMG
IMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTK
YTSAK

Sequence of entity 4 (R), FASTA

>7V99_4 Telomerase RNA component (chains R)
GGGUUGCGGAGGGUGGGCCUGGGAGGGGUGGUGGCCAUUUUUUGUCUAACCCUAACUGAG
AAGGGCGUAGGCGCCGUGCUUUUGCUCCCCGCGCGCUGUUUUUCUCGCUGACUUUCAGCG
GGCGGAAAAGCCUCGGCCUGCCGCCUUCCACCGUUCAUUCUAGAGCAAACAAAAAAUGUC
AGCUGCUGGCCCGUUCGCCCCUCCCGGGGACCUGCGGCGGGUCGCCUGCCCAGCCCCCGA
ACCCCGCCUGGAGGCCGCGGUCGGCCCGGGGCUUCUCCGGAGGCACCCACUGCCACCGCG
AAGAGUUGGGCUCUGUCAGCCGCGGGUCUCUCGGGGGCGAGGGCGAGGUUCAGGCCUUUC
AGGCCGCAGGAAGAGGAACGGAGCGAGUCCCCGCGCGCGGCGCGAUUCCCUGAGCUGUGG
GACGUGCACCCAGGACUCGGCUCACACAUGC

Sequence of entity 5 (S), FASTA

>7V99_5 Primer DNA (chains S)
TTTTTTTTTTTTTTTTTTTTAGGG

Primary citation

Zipper head mechanism of telomere synthesis by human telomerase. Wan, F., Ding, Y., Zhang, Y. et al. Cell Res (2021) 31:1275-1290. DOI 10.1038/s41422-021-00586-7 · PubMed

Other PDB entries of the same protein (UniProt O14746 (AlphaFold model), which also has an AlphaFold model), best resolution first:

5MEO 1.77 Å, Human Leukocyte Antigen presenting ILGKFLHRL
5MER 1.88 Å, Human Leukocyte Antigen A02 presenting ILAKFLHEL
5MEQ 2.27 Å, Human Leukocyte Antigen A02 presenting ILAKFLHTL
5UGW 2.31 Å, Structure of the human telomerase thumb domain
4B18 2.52 Å, The crystal structure of human Importin alpha 5 with TERT NLS peptide
9Q16 2.7 Å, Human telomerase catalytic core with shelterin protein TPP1, dGpNHpp and DNA primer…
5MEP 2.71 Å, Human Leukocyte Antigen A02 presenting ILGKFLHWL
4MNQ 2.74 Å, TCR-peptide specificity overrides affinity enhancing TCR-MHC interactions
2BCK 2.8 Å, Crystal Structure of HLA-A*2402 Complexed with a telomerase peptide
9Q12 2.8 Å, Human telomerase catalytic core with shelterin protein TPP1 and DNA primer ending in TTAG
5MEN 2.81 Å, Human Leukocyte Antigen A02 presenting ILAKFLHWL, in complex with cognate T-Cell Receptor
9Q10 2.9 Å, Human telomerase catalytic core with shelterin protein TPP1, dTpNHpp and DNA primer…

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