7W3Z: Human Gastrin Releasing Peptide Receptor

Cryo-EM Structure of Human Gastrin Releasing Peptide Receptor in complex with the agonist Gastrin Releasing Peptide and Gq heterotrimers. Determined by electron microscopy at 3.0 Å resolution. Released 22 Feb 2023.

Method: Electron microscopy
Resolution: 3.0 Å
Organisms: Escherichia coli, Homo sapiens
Chains: 6
Atoms: 10,221
Mol. weight: 225.53 kDa
Released: 22 Feb 2023

Explore 7W3Z in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7W3Z contains 43 α-helices and 66 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 13 helices, 3 β-strands

Element	Residues	Length	Sheet
α-helix	38-68	31
α-helix	74-88	15
α-helix	89-93	5
α-helix	94-102	9
α-helix	109-143	35
α-helix	153-178	26
β-strand	180-186	7	1
β-strand	192-197	6	1
α-helix	206-215	10
α-helix	216-220	5
α-helix	221-242	22
α-helix	250-252	3
α-helix	253-289	37
β-strand	293-294	2	1
α-helix	298-325	28
α-helix	327-336	10

Chain B: 20 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	13-37	25
β-strand	39-44	6	2
α-helix	48-52	5
α-helix	55-63	9
α-helix	69-97	29
α-helix	104-115	12
α-helix	126-137	12
α-helix	139-146	8
α-helix	148-150	3
α-helix	157-162	6
α-helix	164-168	5
α-helix	176-180	5
β-strand	190-196	7	2
β-strand	199-205	7	2
α-helix	213-215	3
α-helix	217-220	4
β-strand	225-231	7	2
α-helix	232-234	3
β-strand	238	1	3
β-strand	246	1	3
α-helix	247-259	13
α-helix	262-264	3
β-strand	268-274	7	2
α-helix	276-285	10
α-helix	288-291	4
α-helix	302-314	13
β-strand	324-328	5	2
α-helix	336-355	20

Chain C: 4 helices, 29 β-strands

Element	Residues	Length	Sheet
α-helix	4-24	21
α-helix	30-33	4
β-strand	43	1	4
β-strand	47-52	6	5
β-strand	58-63	6	6
β-strand	69-74	6	6
β-strand	78-83	6	6
β-strand	89-94	6	6
α-helix	95	1
β-strand	100-105	6	7
β-strand	111-116	6	7
β-strand	121-125	5	7
β-strand	134-139	6	7
β-strand	146-151	6	8
β-strand	156-161	6	8
β-strand	166-170	5	8
β-strand	175-180	6	8
α-helix	186	1
β-strand	187-192	6	9
β-strand	198-203	6	9
β-strand	208-212	5	9
β-strand	218-222	5	9
β-strand	229-234	6	10
β-strand	240-245	6	10
β-strand	250-254	5	10
β-strand	260-264	5	10
β-strand	273-278	6	4
β-strand	284-289	6	4
β-strand	294-298	5	4
β-strand	303-308	6	4
β-strand	318-320	3	5
β-strand	327-329	3	5
β-strand	335-339	5	5

Chain D: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	9-23	15
α-helix	30-43	14
α-helix	53-55	3

Chain H: 3 helices, 26 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	11
β-strand	11-12	2	12
β-strand	18-25	8	11
α-helix	29-31	3
β-strand	33-39	7	13
β-strand	45-51	7	13
β-strand	58-60	3	13
β-strand	65	1	11
β-strand	68-73	6	11
β-strand	78-83	6	11
β-strand	92-99	8	13
β-strand	110-111	2	13
β-strand	115-117	3	13
β-strand	118-119	2	12
β-strand	140-141	2	14
β-strand	147-148	2	15
β-strand	155-161	7	14
β-strand	166	1	16
β-strand	172	1	16
β-strand	174-179	6	15
β-strand	185-190	6	15
β-strand	194-195	2	15
α-helix	196	1
β-strand	203-207	5	14
β-strand	211-216	6	14
α-helix	221-223	3
β-strand	225-231	7	15
β-strand	239	1	15
β-strand	243-246	4	15

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Maltodextrin-binding protein,Gastrin-releasing peptide receptor	A	protein	897	Escherichia coli, Homo sapiens	P0AEX9 (AlphaFold model), P30550 (AlphaFold model)
Gastrin Releasing Peptide PRGNHWAVGHLM(NH2)	L	protein	13	Homo sapiens	P07492 (AlphaFold model)
Guanine nucleotide-binding protein G(q) subunit alpha	B	protein	353	Homo sapiens	P50148 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	C	protein	380	Homo sapiens	P62873
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	D	protein	81	Homo sapiens	P59768
ScFv16	H	protein	303	Homo sapiens

Sequence of entity 1 (A), FASTA

>7W3Z_1 Maltodextrin-binding protein,Gastrin-releasing peptide receptor (chains A)
MKTIIALSYIFCLVFADYKDDDDKHHHHHHHHHHENLYFQGKIEEGKLVIWINGDKGYNG
LAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEI
TPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKEL
KAKGKSALMFNLQEPYFTWPLIAADGGYAFKYAAGKYDIKDVGVDNAGAKAGLTFLVDLI
KNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSAVNYGVTVLPTFKGQPSKPFV
GVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAA
TMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTHSADLPVNDDWSH
PGILYVIPAVYGVIILIGLIGNITLIKIFCTVKSMRNVPNLFISSLALGDLLLLITCAPV
DASRYLADRWLFGRIGCKLIPFIQLTSVGVSVFTLTALSADRYKAIVRPMDIQASHALMK
ACLKAAFIWIISMLLAIPEAVFSDLHPFHEESTNQTFISCAPYPHSNELHPKIHSMASFL
VFYVIPLSIISVYYYFIAKNLIQSAYNLPVEGNIHVKKQIESRKRLAKTVLVFVGLFAFC
WLPNHVIYLYRSYHYSEVDTSMLHFVTSICARLLAFTNSCVNPFALYLLSKSFRKQFNTQ
LLCCQGSSGGGGSGGGGSSGFTLEDFVGDWEQTAAYNLDQVLEQGGVSSLLQNLAVSVTP
IQRIVRSGENALKIDIHVIIPYEGLSADQMAQIEEVFKVVYPVDDHHFKVILPYGTLVID
GVTPNMLNYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLITPDGSMLFRVTINS

Sequence of entity 2 (L), FASTA

>7W3Z_2 Gastrin Releasing Peptide PRGNHWAVGHLM(NH2) (chains L)
PRGNHWAVGHLMX

Sequence of entity 3 (B), FASTA

>7W3Z_3 Guanine nucleotide-binding protein G(q) subunit alpha (chains B)
MGCTLSAEDKAAVERSKMIDRNLREDGEKARRELKLLLLGTGESGKSTFIKQMRIIHGSG
YSDEDKRGFTKLVYQNIFTAMQAMIRAMDTLKIPYKYEHNKAHAQLVREVDVEKVSAFEN
PYVDAIKSLWNDPGIQECYDRRREYQLSDSTKYYLNDLDRVADPAYLPTQQDVLRVQVPT
TGIIEYPFDLQSVIFRMVDVGGLRSERRKWIHCFENVTSIMFLVALSEYDQVLVESDNEN
RMEESKALFRTIITYPWFQNSSVILFLNKKDLLEEKIMYSHLVDYFPEYDGPQRDAQAAR
EFILKMFVDLNPDSDKIIYSHFTCATDTENIRFVFAAVKDTILQLNLKEYNLV

Sequence of entity 4 (C), FASTA

>7W3Z_4 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains C)
MHHHHHHHHHHGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMR
TRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYA
PSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTC
ALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHE
SDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLL
AGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWNGSSGGG
GSGGGGSSGVSGWRLFKKIS

Sequence of entity 5 (D), FASTA

>7W3Z_5 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains D)
MHHHHHHHHHHASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPL
LTPVPASENPFREKKFFCAIL

Sequence of entity 6 (H), FASTA

>7W3Z_6 ScFv16 (chains H)
MLLVNQSHQGFNKEHTSKMVSAIVLYVLLAAAAHSAFADVQLVESGGGLVQPGGSRKLSC
SASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYYADTVKGRFTISRDDPKNTLFLQ
MTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVSSGGGGSGGGGSGGGGSDIVMTQ
ATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQRPGQSPQLLIYRMSNLASGVPDR
FSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFGAGTKLELKENLYFQGHHHHHHH
HHH

Primary citation

Structures of human gastrin-releasing peptide receptors bound to antagonist and agonist for cancer and itch therapy. Peng, S., Zhan, Y., Zhang, D. et al. Proc Natl Acad Sci U S A (2023) 120:e2216230120-e2216230120. DOI 10.1073/pnas.2216230120 · PubMed

Other PDB entries of the same protein (UniProt P0AEX9 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8C8F 1.15 Å, Crystal structure of the E. coli maltodextrin-binding protein
4EXK 1.28 Å, A chimera protein containing MBP fused to the C-terminal domain of the uncharacterized…
3Q27 1.3 Å, Cyrstal structure of human alpha-synuclein (32-57) fused to maltose binding protein (MBP)
7KD4 1.31 Å, Structure of the C-terminal domain of the Menangle virus phosphoprotein (residues 329…
5M13 1.37 Å, Synthetic nanobody in complex with MBP
5HZ7 1.43 Å, High-resolution crystal structure of the minor DNA-binding pilin ComP from Neisseria…
5H7Q 1.45 Å, Crystal structure of human MNDA PYD domain with MBP tag
6XDS 1.47 Å, Crystal structure of MBP-TREM2 Ig domain fusion with fragment,…
4IRL 1.47 Å, X-ray structure of the CARD domain of zebrafish GBP-NLRP1 like protein
8SVY 1.47 Å, MBP-Mcl1 in complex with ligand 10
3MP6 1.48 Å, Complex Structure of Sgf29 and dimethylated H3K4
9CLC 1.48 Å, Crystal structure of maltose binding protein (Apo), mutant Trp10 to 4-Cyanotryptophan

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