Crystal structure of GSDMB in complex with Ipah7.8. Determined by X-ray diffraction at 2.7 Å resolution. Released 11 Jan 2023.
Explore 7WJQ in 3D Show helices and sheets RCSB PDB PDBe
7WJQ contains 30 α-helices and 24 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 24-35 | 12 | |
| α-helix | 44-56 | 13 | |
| β-strand | 61-63 | 3 | 1 |
| α-helix | 75-76 | 2 | |
| β-strand | 81-83 | 3 | 1 |
| α-helix | 93-96 | 4 | |
| β-strand | 101-103 | 3 | 1 |
| α-helix | 113-116 | 4 | |
| β-strand | 121-123 | 3 | 1 |
| α-helix | 134-136 | 3 | |
| β-strand | 141-143 | 3 | 1 |
| α-helix | 154-156 | 3 | |
| β-strand | 161-163 | 3 | 1 |
| α-helix | 173-175 | 3 | |
| β-strand | 181-183 | 3 | 1 |
| α-helix | 192-196 | 5 | |
| β-strand | 204-207 | 4 | 1 |
| α-helix | 218-220 | 3 | |
| β-strand | 227-230 | 4 | 1 |
| α-helix | 238-247 | 10 | |
| β-strand | 258-260 | 3 | 1 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 4-17 | 14 | |
| β-strand | 23-24 | 2 | 2 |
| α-helix | 31-33 | 3 | |
| β-strand | 38-45 | 8 | 2 |
| β-strand | 48-57 | 10 | 2 |
| α-helix | 58-61 | 4 | |
| β-strand | 62 | 1 | 3 |
| α-helix | 77-88 | 12 | |
| β-strand | 90-99 | 10 | 2 |
| α-helix | 100-101 | 2 | |
| β-strand | 104-106 | 3 | 4 |
| β-strand | 117-125 | 9 | 2 |
| α-helix | 127-131 | 5 | |
| α-helix | 137 | 1 | |
| β-strand | 138 | 1 | 3 |
| α-helix | 139 | 1 | |
| α-helix | 144-147 | 4 | |
| α-helix | 152-154 | 3 | |
| β-strand | 155-164 | 10 | 2 |
| β-strand | 168-172 | 5 | 4 |
| β-strand | 195-199 | 5 | 4 |
| α-helix | 200 | 1 | |
| β-strand | 204-211 | 8 | 2 |
| α-helix | 249-261 | 13 | |
| α-helix | 265-276 | 12 | |
| α-helix | 282-298 | 17 | |
| α-helix | 308-312 | 5 | |
| β-strand | 315 | 1 | 5 |
| β-strand | 321 | 1 | 5 |
| α-helix | 323-337 | 15 | |
| α-helix | 343-352 | 10 | |
| α-helix | 355-368 | 14 | |
| α-helix | 384-402 | 19 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| Probable E3 ubiquitin-protein ligase ipaH7.8 | A | protein | 242 | Shigella flexneri | P18014 (AlphaFold model) |
| Isoform 2 of Gasdermin-B | B | protein | 389 | Homo sapiens | Q8TAX9 (AlphaFold model) |
>7WJQ_1 Probable E3 ubiquitin-protein ligase ipaH7.8 (chains A) SNEHYLRILTEWEKNSSPGEERGIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISAL IVENNKLTSLPKLPAFLKELNADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSL FVENNRLYNLPALPEKLKFLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIR QKEYYFHFNQITTLPESFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGPQIYFSD SD
>7WJQ_2 Isoform 2 of Gasdermin-B (chains B) MFSVFEEITRIVVKEMDAGGDMIAVRSLVDADRFRCFHLVGEKRTFFGCRHYTTGLTLMD ILDTDGDKWLDELDSGLQGQKAEFQILDNVDSTGELIVRLPKEITISGSFQGFHHQKIKI SENRISQQYLATLENRKLKRELPFSFRSINTRENLYLVTETLETVKEETLKSDRQYKFWS QISQGHLSYKHKGQREVTIPPNRVLSYRVKQLVFPNKETMSASLGSEDSRNMKEKLEDME SVLKDLTEEKRKDVLNSLAKCLGKEDIRQDLEQRVSEVLISGELHMEDPDKPLLSSLFNA AGVLVEARAKAILDFLDALLELSEEQQFVAEALEKGTLPLLKDQVKSVMEQNWDELASSP PDMDYDPEARILCALYVVVSILLELAEGP
Insights into the GSDMB-mediated cellular lysis and its targeting by IpaH7.8. Yin, H., Zheng, J., He, Q. et al. Nat Commun (2023) 14:61. DOI 10.1038/s41467-022-35725-0
Other PDB entries of the same protein (UniProt P18014 (AlphaFold model), which also has an AlphaFold model), best resolution first:
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