7WVX: Human formyl peptide receptor 2

Cryo-EM structure of the human formyl peptide receptor 2 in complex with fhumanin and Gi2. Determined by electron microscopy at 2.8 Å resolution. Released 13 Apr 2022.

Method: Electron microscopy
Resolution: 2.8 Å
Organism: Homo sapiens
Chains: 5
Atoms: 7,070
Mol. weight: 146.54 kDa
Released: 13 Apr 2022

Explore 7WVX in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7WVX contains 28 α-helices and 38 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 10 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	6-30	25
β-strand	33-39	7	3
α-helix	46-51	6
β-strand	186-192	7	3
β-strand	195-201	7	3
α-helix	209-212	4
α-helix	213-216	4
β-strand	221-227	7	3
α-helix	228-230	3
α-helix	243-255	13
α-helix	258-260	3
β-strand	264-270	7	3
α-helix	272-279	8
α-helix	297-311	15
β-strand	320-324	5	3
α-helix	332-352	21

Chain B: 2 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	4-25	22
α-helix	30-34	5
β-strand	47-51	5	4
β-strand	58-63	6	5
β-strand	69-74	6	5
β-strand	78-83	6	5
β-strand	88-94	7	5
β-strand	100-105	6	6
β-strand	111-116	6	6
β-strand	121-125	5	6
β-strand	135-139	5	6
β-strand	149-151	3	7
β-strand	156-159	4	7
β-strand	166-170	5	7
β-strand	175-180	6	7
β-strand	187-192	6	8
β-strand	198-203	6	8
β-strand	208-212	5	8
β-strand	218-222	5	8
β-strand	229-234	6	9
β-strand	240-245	6	9
β-strand	250-254	5	9
β-strand	259-264	6	9
β-strand	276-278	3	10
β-strand	284-287	4	10
β-strand	294-298	5	10
β-strand	304-308	5	10
β-strand	317-320	4	4
β-strand	327-330	4	4
β-strand	336-339	4	4

Chain C: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	11-24	14
α-helix	30-43	14
α-helix	45-47	3

Chain R: 13 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	22-54	33
α-helix	59-85	27
α-helix	95-128	34
α-helix	130-136	7
α-helix	139-162	24
β-strand	164	1	1
β-strand	167-168	2	2
β-strand	174-175	2	2
β-strand	178	1	1
α-helix	181-184	4
α-helix	187-204	18
α-helix	205-211	7
α-helix	212-231	20
α-helix	238-266	29
α-helix	268-273	6
α-helix	279-302	24
α-helix	307-316	10

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Humanin	L	protein	24	Homo sapiens	Q8IVG9 (AlphaFold model)
Soluble cytochrome b562,N-formyl peptide receptor 2	R	protein	513	Homo sapiens	P0ABE7 (AlphaFold model), P25090 (AlphaFold model)
Guanine nucleotide-binding protein G(i) subunit alpha-2	A	protein	355	Homo sapiens	P04899 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	351	Homo sapiens	P62873
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	C	protein	71	Homo sapiens	P59768

Sequence of entity 1 (L), FASTA

>7WVX_1 Humanin (chains L)
MAPRGFSCLLLLTSEIDLPVKRRA

Sequence of entity 2 (R), FASTA

>7WVX_2 Soluble cytochrome b562,N-formyl peptide receptor 2 (chains R)
GAPADLEDNWETLNDNLKVIEKADNAAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPE
MKDFRHGFDILVGQIDDALKLANEGKVKEAQAAAEQLKTTRNAYIQKYLGSGSENLYFQS
ETNFSTPLNEYEEVSYESAGYTVLRILPLVVLGVTFVLGVLGNGLVIWVAGFRMTRTVTT
ICYLNLALADFSFTATLPFLIVSMAMGEKWPFGWFLCKLIHIVVDINLFGSVFLIGFIAL
DRCICVLHPVWAQNHRTVSLAMKVIVGPWILALVLTLPVFLFLTTVTIPNGDTYCTFNFA
SWGGTPEERLKVAITMLTARGIIRFVIGFLLPMSIVAICYGLIAAKIHKKGMIKSSRPLR
VLTAVVASFFICWFPFQLVALLGTVWLKEMLFYGKYKIIDILVNPTSSLAFFNSCLNPML
YVFVGQDFRERLIHSLPTSLERALSEDSAPTNDTAANSASPPAETEFLEVLFQGPGSWSH
PQFEKGSGAGASAGSWSHPQFEKGSDYKDDDDK

Sequence of entity 3 (A), FASTA

>7WVX_3 Guanine nucleotide-binding protein G(i) subunit alpha-2 (chains A)
MGCTVSAEDKAAAERSKMIDKNLREDGEKAAREVKLLLLGAGESGKNTIVKQMKIIHEDG
YSEEECRQYRAVVYSNTIQSIMAIVKAMGNLQIDFADPSRADDARQLFALSCTAEEQGVL
PDDLSGVIRRLWADHGVQACFGRSREYQLNDSAAYYLNDLERIAQSDYIPTQQDVLRTRV
KTTGIVETHFTFKDLHFKMFDVGAQRSERKKWIHCFEGVTAIIFCVALSAYDLVLAEDEE
MNRMHASMKLFDSICNNKWFTDTSIILFLNKKDLFEEKITHSPLTICFPEYTGANKYDEA
ASYIQSKFEDLNKRKDTKEIYTHFTCSTDTKNVQFVFDAVTDVIIKNNLKDCGLF

Sequence of entity 4 (B), FASTA

>7WVX_4 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRR
TLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSG
NYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALW
DIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDI
NAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGY
DDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 5 (C), FASTA

>7WVX_5 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains C)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Primary citation

Structural basis of FPR2 in recognition of A beta 42 and neuroprotection by humanin. Zhu, Y., Lin, X., Zong, X. et al. Nat Commun (2022) 13:1775-1775. DOI 10.1038/s41467-022-29361-x · PubMed

Other PDB entries of the same protein (UniProt Q8IVG9 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1Y32 NMR structure of humanin in 30% TFE solution
2GD3 NMR structure of S14G-humanin in 30% TFE solution
5GIW Solution NMR structure of Humanin containing a D-isomerized serine residue

Browse structure collections

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