7XMT: Somatostatin receptor type 4

CryoEM structure of somatostatin receptor 4 (SSTR4) with Gi1 and J-2156. Determined by electron microscopy at 2.8 Å resolution. Released 3 Aug 2022.

Method: Electron microscopy
Resolution: 2.8 Å
Organisms: Homo sapiens, Mus musculus
Chains: 5
Atoms: 8,137
Mol. weight: 159.74 kDa
Ligands: I8B
Released: 3 Aug 2022

Explore 7XMT in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7XMT contains 33 α-helices and 70 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 9 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	9-32	24
β-strand	34-38	5	2
α-helix	183-184	2
β-strand	185-191	7	2
β-strand	194-200	7	2
α-helix	208-210	3
β-strand	220-226	7	2
α-helix	227-229	3
β-strand	233	1	3
α-helix	234	1
β-strand	241	1	3
α-helix	242-254	13
β-strand	263-269	7	2
α-helix	271-278	8
α-helix	296-307	12
β-strand	319-324	6	2
α-helix	332-351	20

Chain B: 2 helices, 29 β-strands

Element	Residues	Length	Sheet
β-strand	47-51	5	15
β-strand	58-62	5	16
β-strand	69-74	6	16
β-strand	78-83	6	16
β-strand	89-94	6	16
β-strand	100-105	6	17
β-strand	111-116	6	17
β-strand	123-125	3	17
α-helix	133	1
β-strand	134-137	4	17
α-helix	145-146	2
β-strand	149-151	3	18
β-strand	157-160	4	18
β-strand	166-169	4	18
β-strand	176-180	5	18
β-strand	187	1	19
β-strand	198-202	5	20
β-strand	203	1	19
β-strand	208-212	5	20
β-strand	221-222	2	20
β-strand	229-234	6	21
β-strand	240-245	6	21
β-strand	249-254	6	21
β-strand	262-263	2	21
β-strand	273-277	5	22
β-strand	284-289	6	22
β-strand	293-298	6	22
β-strand	304-309	6	22
β-strand	315-320	6	15
β-strand	327-331	5	15
β-strand	336-339	4	15

Chain C: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	31-34	4
α-helix	35-37	3
α-helix	38-41	4

Chain R: 15 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	47-71	25
α-helix	72-76	5
α-helix	81-96	16
α-helix	100-108	9
α-helix	119-149	31
α-helix	151-154	4
α-helix	160-178	19
α-helix	180-183	4
β-strand	185	1	1
β-strand	200	1	1
α-helix	205-216	12
α-helix	217-221	5
α-helix	222-244	23
α-helix	252-283	32
α-helix	294-306	13
α-helix	308-312	5
α-helix	316-319	4

Chain S: 4 helices, 31 β-strands

Element	Residues	Length	Sheet
β-strand	3-6	4	4
β-strand	12	1	5
β-strand	17	1	6
β-strand	20-25	6	4
α-helix	29-31	3
β-strand	34-38	5	7
β-strand	46-51	6	7
β-strand	58-60	3	7
β-strand	68-73	6	4
β-strand	78-83	6	4
β-strand	84	1	6
α-helix	88-90	3
β-strand	92	1	8
β-strand	95-97	3	7
β-strand	111	1	7
β-strand	117	1	8
β-strand	119	1	5
β-strand	140-141	2	9
β-strand	146-148	3	10
β-strand	155-159	5	11
β-strand	160-161	2	9
β-strand	166	1	12
β-strand	172	1	12
β-strand	175-179	5	13
β-strand	186-187	2	13
β-strand	189-190	2	14
β-strand	194-195	2	14
α-helix	196	1
β-strand	203-208	6	11
β-strand	211-216	6	11
β-strand	226-230	5	13
α-helix	237	1
β-strand	238	1	7
β-strand	243	1	13
β-strand	244-246	3	10

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Somatostatin receptor type 4	R	protein	375	Homo sapiens	P31391 (AlphaFold model)
Guanine nucleotide-binding protein G(i) subunit alpha-1	A	protein	354	Homo sapiens	P63096 (AlphaFold model)
single chain variable fragment of antibody	S	protein	292	Mus musculus
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	C	protein	71	Homo sapiens	P59768 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	351	Homo sapiens	P62873 (AlphaFold model)

Sequence of entity 1 (R), FASTA

>7XMT_1 Somatostatin receptor type 4 (chains R)
DYKDDDDGAPSAPSTLPPGGEEGLGTAWPSAANASSAPAEAEEAVAGPGDARAAGMVAIQ
CIYALVCLVGLVGNALVIFVILRYAKMKTATNIYLLNLAVADELFMLSVPFVASSAALRH
WPFGSVLCRAVLSVDGLNMFTSVFCLTVLSVDRYVAVVHPLRAATYRRPSVAKLINLGVW
LASLLVTLPIAIFADTRPARGGQAVACNLQWPHPAWSAVFVVYTFLLGFLLPVLAIGLCY
LLIVGKMRAVALRAGWQQRRRSEKKITRLVLMFVVVFVLCWMPFYVVQLLNLFVTSLDAT
VNHVSLILSYANSCANPILYGFLSDNFRRFFQRVLCLEFLEVLFQGPWSHPQFEKGGGSG
GGSGGSAWSHPQFEK

Sequence of entity 2 (A), FASTA

>7XMT_2 Guanine nucleotide-binding protein G(i) subunit alpha-1 (chains A)
MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKCTIVKQMKIIHEAG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TTGIVETHFTFKDLHFKMFDVTAQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEM
NRMHASMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAA
AYIQCQFEDLNKRKDTKEIYTHFTCSTDTKNVQFVFDAVTDVIIKNNLKDCGLF

Sequence of entity 3 (S), FASTA

>7XMT_3 single chain variable fragment of antibody (chains S)
MKTIIALSYIFCLVFADYKDDDDGAPSEPDVQLVESGGGLVQPGGSRKLSCSASGFAFSS
FGMHWVRQAPEKGLEWVAYISSGSGTIYYADTVKGRFTISRDDPKNTLFLQMTSLRSEDT
AMYYCVRSIYYYGSSPFDFWGQGTTLTVSSGGGGSGGGGSGGGGSDIVMTQATSSVPVTP
GESVSISCRSSKSLLHSNGNTYLYWFLQRPGQSPQLLIYRMSNLASGVPDRFSGSGSGTA
FTLTISRLEAEDVGVYYCMQHLEYPLTFGAGTKLELEFLEVLFQGPHHHHHH

Sequence of entity 4 (C), FASTA

>7XMT_4 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains C)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 5 (B), FASTA

>7XMT_5 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRR
TLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSG
NYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALW
DIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDI
NAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGY
DDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Ligands and cofactors

ID	Name	Formula	Copies
I8B	(2~{S})-2-[[(2~{S})-4-azanyl-2-[(4-methylnaphthalen-1-yl)sulfonylamino]butanoyl…	C24 H28 N4 O4 S	1

Primary citation

Structural insights into ligand recognition and selectivity of somatostatin receptors. Zhao, W., Han, S., Qiu, N. et al. Cell Res (2022) 32:761-772. DOI 10.1038/s41422-022-00679-x · PubMed

Other PDB entries of the same protein (UniProt P31391 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7XMS 2.9 Å, CryoEM structure of somatostatin receptor 4 (SSTR4) in complex with Gi1 and its…

Browse structure collections

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