7XOV: PDB entry 7XOV
Structural insights into human brain gut peptide cholecystokinin receptors. Determined by electron microscopy at 3.0 Å resolution. Released 20 Jul 2022.
- Method
- Electron microscopy
- Resolution
- 3.0 Å
- Organisms
- Homo sapiens, synthetic construct
- Chains
- 5
- Atoms
- 8,218
- Mol. weight
- 151.55 kDa
- Ligands
- IA1
- Released
- 20 Jul 2022
Explore 7XOV in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
7XOV contains 38 α-helices and 50 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain A: 10 helices, 7 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 13-37 | 25 | |
| β-strand | 40-47 | 8 | 1 |
| α-helix | 53-59 | 7 | |
| β-strand | 208-214 | 7 | 1 |
| β-strand | 217-223 | 7 | 1 |
| α-helix | 235-238 | 4 | |
| β-strand | 243-249 | 7 | 1 |
| α-helix | 265-277 | 13 | |
| β-strand | 286-292 | 7 | 1 |
| α-helix | 294-302 | 9 | |
| α-helix | 313-317 | 5 | |
| α-helix | 326-327 | 2 | |
| α-helix | 332-349 | 18 | |
| β-strand | 359-363 | 5 | 1 |
| α-helix | 374-388 | 15 | |
| α-helix | 389-391 | 3 | |
| β-strand | 393 | 1 | 2 |
Chain B: 4 helices, 28 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 4-23 | 20 | |
| α-helix | 31-34 | 4 | |
| α-helix | 38-39 | 2 | |
| β-strand | 47-51 | 5 | 3 |
| β-strand | 58-63 | 6 | 4 |
| β-strand | 69-74 | 6 | 4 |
| β-strand | 78-83 | 6 | 4 |
| β-strand | 89-94 | 6 | 4 |
| β-strand | 100-105 | 6 | 5 |
| β-strand | 111-116 | 6 | 5 |
| β-strand | 120-125 | 6 | 5 |
| β-strand | 134-140 | 7 | 5 |
| β-strand | 146-152 | 7 | 6 |
| β-strand | 156-161 | 6 | 6 |
| β-strand | 166-170 | 5 | 6 |
| β-strand | 175-180 | 6 | 6 |
| β-strand | 187-192 | 6 | 7 |
| β-strand | 198-203 | 6 | 7 |
| β-strand | 207-212 | 6 | 7 |
| β-strand | 217-223 | 7 | 7 |
| β-strand | 229-234 | 6 | 8 |
| β-strand | 240-245 | 6 | 8 |
| β-strand | 250-254 | 5 | 8 |
| β-strand | 259-264 | 6 | 8 |
| α-helix | 271-272 | 2 | |
| β-strand | 273-278 | 6 | 9 |
| β-strand | 284-289 | 6 | 9 |
| β-strand | 293-298 | 6 | 9 |
| β-strand | 303-309 | 7 | 9 |
| β-strand | 315-320 | 6 | 3 |
| β-strand | 327-331 | 5 | 3 |
| β-strand | 336-339 | 4 | 3 |
Chain G: 6 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 9-22 | 14 | |
| α-helix | 27-29 | 3 | |
| α-helix | 30-43 | 14 | |
| α-helix | 45-47 | 3 | |
| α-helix | 53-55 | 3 | |
| α-helix | 56-58 | 3 | |
Chain N: 2 helices, 10 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 3-7 | 5 | 10 |
| β-strand | 10-11 | 2 | 11 |
| β-strand | 18-25 | 8 | 10 |
| β-strand | 34-39 | 6 | 11 |
| β-strand | 45-51 | 7 | 11 |
| β-strand | 57-60 | 4 | 11 |
| α-helix | 62-64 | 3 | |
| β-strand | 68-73 | 6 | 10 |
| β-strand | 78-83 | 6 | 10 |
| α-helix | 88-90 | 3 | |
| β-strand | 92-98 | 7 | 11 |
| β-strand | 120-125 | 6 | 11 |
Chain R: 16 helices, 5 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 44-68 | 25 | |
| α-helix | 75-94 | 20 | |
| α-helix | 95-99 | 5 | |
| α-helix | 100-104 | 5 | |
| α-helix | 111-144 | 34 | |
| α-helix | 146-152 | 7 | |
| α-helix | 155-172 | 18 | |
| β-strand | 180-182 | 3 | 12 |
| β-strand | 185 | 1 | 13 |
| β-strand | 192 | 1 | 13 |
| β-strand | 195-197 | 3 | 12 |
| α-helix | 205-213 | 9 | |
| α-helix | 214-219 | 6 | |
| α-helix | 220-243 | 24 | |
| α-helix | 302-338 | 37 | |
| α-helix | 340-349 | 10 | |
| α-helix | 352-360 | 9 | |
| α-helix | 362-367 | 6 | |
| α-helix | 368-372 | 5 | |
| β-strand | 374 | 1 | 2 |
| α-helix | 379-384 | 6 | |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Isoform Gnas-2 of Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | A | protein | 380 | Homo sapiens | P63092 (AlphaFold model) |
| Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | B | protein | 338 | Homo sapiens | P62873 (AlphaFold model) |
| Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | G | protein | 71 | Homo sapiens | P59768 (AlphaFold model) |
| Nanobody 35 | N | protein | 126 | synthetic construct | |
| Cholecystokinin receptor type A | R | protein | 428 | Homo sapiens | P32238 (AlphaFold model) |
Sequence of entity 1 (A), FASTA
>7XOV_1 Isoform Gnas-2 of Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKNTIVKQM
RILHVNGFNGDSEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSV
MNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSD
QDLLRCRVLTSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFNDVTAIIFVVASSSYN
MVIREDNQTNRLQAALKLFDSIWNNKWLRDTSVILFLNKQDLLAEKVLAGKSKIEDYFPE
FARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRR
VFNDCRDIIQRMHLRQYELL
Sequence of entity 2 (B), FASTA
>7XOV_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
ELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAMH
WGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNICS
IYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTFTG
HTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNAFA
TGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALKA
DRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN
Sequence of entity 3 (G), FASTA
>7XOV_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL
Sequence of entity 4 (N), FASTA
>7XOV_4 Nanobody 35 (chains N)
QVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISY
TGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQ
GTQVTV
Sequence of entity 5 (R), FASTA
>7XOV_5 Cholecystokinin receptor type A (chains R)
MDVVDSLLVNGSNITPPCELGLENETLFCLDQPRPSKEWQPAVQILLYSLIFLLSVLGNT
LVITVLIRNKRMRTVTNIFLLSLAVSDLMLCLFCMPFNLIPNLLKDFIFGSAVCKTTTYF
MGTSVSVSTFNLVAISLERYGAICKPLQSRVWQTKSHALKVIAATWCLSFTIMTPYPIYS
NLVPFTKNNNQTANMCRFLLPNDVMQQSWHTFLLLILFLIPGIVMMVAYGLISLELYQGI
KFEASQKKSAKERKPSTTSSGKYEDSDGCYLQKTRPPRKLELRQLSTGSSSRANRIRSNS
SAANLMAKKRVIRMLIVIVVLFFLCWMPIFSANAWRAYDTASAERRLSGTPISFILLLSY
TSSCVNPIIYCFMNKRFRLGFMATFPCCPNPGPPGARGEVGEEEEGGTTGASLSRFSYSH
MSASVPPQ
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| IA1 | 2-[2-[[4-(4-chloranyl-2,5-dimethoxy-phenyl)-5-(2-cyclohexylethyl)-1,3-thiazol-2… | C32 H36 Cl N3 O5 S | 1 |
Primary citation
Structural insights into human brain-gut peptide cholecystokinin receptors. Ding, Y., Zhang, H., Liao, Y.Y. et al. Cell Discov (2022) 8:55-55. DOI 10.1038/s41421-022-00420-3 · PubMed
Other PDB entries of the same protein (UniProt P63092 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 7BPH 1.57 Å, Crystal structure of GppNHp-bound GNAS in complex with the cyclic peptide inhibitor GN13
- 6AU6 1.7 Å, Crystal structure of GDP-bound human GNAS R201C mutant
- 8F0K 1.9 Å, Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385
- 7E5E 1.95 Å, Crystal structure of GDP-bound GNAS in complex with the cyclic peptide inhibitor GD20
- 7MBX 1.95 Å, Human Cholecystokinin 1 receptor (CCK1R) Gs complex
- 8F0J 2.0 Å, Calcitonin Receptor in complex with Gs and Pramlintide analogue peptide San45
- 8F2B 2.0 Å, Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
- 9XXT 2.0 Å, Cryo-EM structure of lysophosphatidylserine (18:0)-bound GPR174-Gs complex
- 6X18 2.1 Å, GLP-1 peptide hormone bound to Glucagon-Like peptide-1 (GLP-1) Receptor
- 6X19 2.1 Å, Non peptide agonist CHU-128, bound to Glucagon-Like peptide-1 (GLP-1) Receptor
- 9NTU 2.1 Å, Cryo-EM structure of BETP-GLP-1(9-36)-GLP-1R-Gs complex
- 7RTB 2.14 Å, Peptide-19 bound to the Glucagon-Like Peptide-1 Receptor (GLP-1R)
Browse structure collections
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