7XP6: Class T GPCR in active state

Cryo-EM structure of a class T GPCR in active state. Determined by electron microscopy at 3.01 Å resolution. Released 12 Oct 2022.

Method: Electron microscopy
Resolution: 3.01 Å
Organisms: Homo sapiens, Acetivibrio thermocellus
Chains: 5
Atoms: 8,336
Mol. weight: 184.9 kDa
Ligands: SY9
Released: 12 Oct 2022

Explore 7XP6 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7XP6 contains 29 α-helices and 46 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 9 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	6-35	30
β-strand	37-43	7	1
α-helix	49-60	12
β-strand	72-79	8	1
β-strand	82-89	8	1
α-helix	100-103	4
β-strand	108-114	7	1
α-helix	118-132	15
β-strand	141-147	7	1
α-helix	149-158	10
α-helix	163-166	4
α-helix	168-170	3
α-helix	187-206	20
β-strand	214-218	5	1
α-helix	223-246	24

Chain B: 3 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	4-25	22
α-helix	30-34	5
α-helix	38-39	2
β-strand	45-51	7	2
β-strand	58-63	6	3
β-strand	69-74	6	3
β-strand	78-83	6	3
β-strand	88-94	7	3
β-strand	100-105	6	4
β-strand	111-116	6	4
β-strand	121-125	5	4
β-strand	134-139	6	4
β-strand	146-151	6	5
β-strand	156-161	6	5
β-strand	166-170	5	5
β-strand	175-180	6	5
β-strand	187-192	6	6
β-strand	198-203	6	6
β-strand	208-212	5	6
β-strand	217-222	6	6
β-strand	229-234	6	7
β-strand	240-245	6	7
β-strand	250-254	5	7
β-strand	259-264	6	7
β-strand	276-278	3	8
β-strand	284-287	4	8
β-strand	294-298	5	8
β-strand	304-308	5	8
β-strand	315-320	6	2
β-strand	327-331	5	2
β-strand	336-341	6	2

Chain G: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	7-23	17
α-helix	30-43	14
α-helix	53-55	3

Chain N: 3 helices, 12 β-strands

Element	Residues	Length	Sheet
β-strand	4-7	4	9
β-strand	12-13	2	10
β-strand	19-26	8	9
α-helix	30-32	3
β-strand	35-40	6	11
β-strand	46-52	7	11
β-strand	59-61	3	11
α-helix	63-65	3
β-strand	69-74	6	9
β-strand	79-84	6	9
α-helix	89-91	3
β-strand	93-99	7	11
β-strand	119	1	11
β-strand	123-125	3	11
β-strand	126-127	2	10

Chain R: 11 helices, 0 β-strands

Element	Residues	Length
α-helix	4-38	35
α-helix	43-70	28
α-helix	77-110	34
α-helix	116-123	8
α-helix	125-153	29
α-helix	174-180	7
α-helix	181-185	5
α-helix	186-212	27
α-helix	219-253	35
α-helix	259-282	24
α-helix	284-300	17

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(t) subunit alpha-3	A	protein	264	Homo sapiens	P63092 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	366	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Homo sapiens	P59768 (AlphaFold model)
Nanobody 35	N	protein	139	Homo sapiens
Endoglucanase H,Taste receptor type 2 member 46,Endoglucanase H,Taste receptor type 2 member…	R	protein	802	Acetivibrio thermocellus, Homo sapiens	P16218 (AlphaFold model), P59540

Sequence of entity 1 (A), FASTA

>7XP6_1 Guanine nucleotide-binding protein G(t) subunit alpha-3 (chains A)
MDYKDDDDKENLYFQSNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAD
NSGKSTIVKQMRILHGGSGGSGGTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFND
VTAIIFVVDSSDYNRLQEALNDFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIED
YFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTQ
NVKFVFDAVTDIIIKENLKDCGLF

Sequence of entity 2 (B), FASTA

>7XP6_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYA
MHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNI
CSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTF
TGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNA
FATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDAL
KADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWNGSSGGGGSGGGGSSGVSGWR
LFKKIS

Sequence of entity 3 (G), FASTA

>7XP6_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 4 (N), FASTA

>7XP6_4 Nanobody 35 (chains N)
MQVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASIS
YTGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRG
QGTQVTVSSHHHHHHEPEA

Sequence of entity 5 (R), FASTA

>7XP6_5 Endoglucanase H,Taste receptor type 2 member 46,Endoglucanase H,Taste receptor type 2 member 46,Bitter taste receptor T2R46 (chains R)
MKTIIALSYIFCLVFADYKDDDDAHHHHHHHHHHENLYFQSGRAMASNYNSGLKIGAWVG
TQPSESAIKSFQELQGRKLDIVHQFINWSTDFSWVRPYADAVYNNGSILMITWEPWEYNT
VDIKNGKADAYITRMAQDMKAYGKEIWLRPLHAANGDWYPWAIGYSSRVNTNETYIAAFR
HIVDIFRANGATNVKWVFNVNCDNVGNGTSYLGHYPGDNYVDYTSIDGYNWGTTQSWGSQ
WQSFDQVFSRAYQALASINKPIIIAEFASAEIGGNKARWITEAYNSIRTSYNKVIAAVWF
HENKETDWRINSSPEALAAYREAIGAITFLPIIFSILIVVTFVIGNFANGFIALVNSIEW
FKRQKISFADQILTALAVSRVGLLWVLVLNWYATELNPAFNSIEVRITAYNVWAVINHFS
NWLATSLSIFYLLKIANFSNLIFLHLKRRVKSVVLVILLGPLLFLVCHLFVINMNQIIWT
KEYEGNMTWKIKLRSAMYLSNTTVTILANLVPFTLTLISFLLLICSLCKHLKKMQLHGKG
SQDPSMKVHIKALQTVTSFLLLCAIYFLSIIMSVWSFESLENKPVFMFCEAIAFSYPSTH
PFILIWGNKKLKQTFLSVLWHVRYWVKGEKPSSSGSGSSGSGSSVFTLEDFVGDWEQTAA
YNLDQVLEQGGVSSLLQNLAVSVTPIQRIVRSGENALKIDIHVIIPYEGLSADQMAQIEE
VFKVVYPVDDHHFKVILPYGTLVIDGVTPNMLNYFGRPYEGIAVFDGKKITVTGTLWNGN
KIIDERLITPDGSMLFRVTINS

Ligands and cofactors

ID	Name	Formula	Copies
SY9	Strychnine	C21 H22 N2 O2	1

Primary citation

Structural basis for strychnine activation of human bitter taste receptor TAS2R46. Xu, W., Wu, L., Liu, S. et al. Science (2022) 377:1298-1304. DOI 10.1126/science.abo1633 · PubMed

Other PDB entries of the same protein (UniProt P63092 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7BPH 1.57 Å, Crystal structure of GppNHp-bound GNAS in complex with the cyclic peptide inhibitor GN13
6AU6 1.7 Å, Crystal structure of GDP-bound human GNAS R201C mutant
8F0K 1.9 Å, Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385
7E5E 1.95 Å, Crystal structure of GDP-bound GNAS in complex with the cyclic peptide inhibitor GD20
7MBX 1.95 Å, Human Cholecystokinin 1 receptor (CCK1R) Gs complex
8F0J 2.0 Å, Calcitonin Receptor in complex with Gs and Pramlintide analogue peptide San45
8F2B 2.0 Å, Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
9XXT 2.0 Å, Cryo-EM structure of lysophosphatidylserine (18:0)-bound GPR174-Gs complex
6X18 2.1 Å, GLP-1 peptide hormone bound to Glucagon-Like peptide-1 (GLP-1) Receptor
6X19 2.1 Å, Non peptide agonist CHU-128, bound to Glucagon-Like peptide-1 (GLP-1) Receptor
9NTU 2.1 Å, Cryo-EM structure of BETP-GLP-1(9-36)-GLP-1R-Gs complex
7RTB 2.14 Å, Peptide-19 bound to the Glucagon-Like Peptide-1 Receptor (GLP-1R)

Browse structure collections

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