Structure of CRL7FBXW8 reveals coupling with CUL1-RBX1/ROC1 for multi-cullin-RING E3-catalyzed ubiquitin ligation. Determined by electron microscopy at 2.8 Å resolution. Released 24 Aug 2022.
Explore 7Z8B in 3D Show helices and sheets RCSB PDB PDBe
7Z8B contains 89 α-helices and 77 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 12-15 | 4 | 1 |
| β-strand | 18-30 | 13 | 1 |
| β-strand | 36-45 | 10 | 1 |
| β-strand | 63-69 | 7 | 1 |
| α-helix | 71-77 | 7 | |
| α-helix | 79-81 | 3 | |
| α-helix | 116-119 | 4 | |
| α-helix | 142-148 | 7 | |
| α-helix | 153-157 | 5 | |
| α-helix | 162-170 | 9 | |
| α-helix | 176-186 | 11 | |
| α-helix | 198-204 | 7 | |
| α-helix | 216-228 | 13 | |
| α-helix | 247-268 | 22 | |
| α-helix | 288-310 | 23 | |
| β-strand | 465-466 | 2 | 2 |
| α-helix | 467-469 | 3 | |
| β-strand | 488 | 1 | 3 |
| α-helix | 491-501 | 11 | |
| α-helix | 506-519 | 14 | |
| α-helix | 526-529 | 4 | |
| β-strand | 536 | 1 | 3 |
| α-helix | 537-546 | 10 | |
| α-helix | 553-561 | 9 | |
| α-helix | 620-625 | 6 | |
| α-helix | 633-640 | 8 | |
| α-helix | 652-661 | 10 | |
| α-helix | 664-671 | 8 | |
| α-helix | 683-693 | 11 | |
| α-helix | 699-701 | 3 | |
| α-helix | 704-706 | 3 | |
| α-helix | 711-714 | 4 | |
| α-helix | 723-738 | 16 | |
| α-helix | 741-749 | 9 | |
| α-helix | 751-755 | 5 | |
| α-helix | 756-760 | 5 | |
| α-helix | 769-810 | 42 | |
| α-helix | 813-815 | 3 | |
| α-helix | 820-828 | 9 | |
| β-strand | 841-846 | 6 | 4 |
| α-helix | 850-856 | 7 | |
| β-strand | 865-866 | 2 | 5 |
| β-strand | 874-880 | 7 | 4 |
| α-helix | 881 | 1 | |
| β-strand | 888-892 | 5 | 6 |
| β-strand | 902-910 | 9 | 4 |
| β-strand | 918-924 | 7 | 4 |
| α-helix | 925-926 | 2 | |
| β-strand | 931-935 | 5 | 6 |
| β-strand | 944-952 | 9 | 4 |
| α-helix | 953-955 | 3 | |
| β-strand | 960-961 | 2 | 5 |
| β-strand | 963-967 | 5 | 6 |
| α-helix | 969-971 | 3 | |
| α-helix | 977-1002 | 26 | |
| α-helix | 1004-1009 | 6 | |
| α-helix | 1010-1028 | 19 | |
| α-helix | 1032-1055 | 24 | |
| α-helix | 1064-1074 | 11 | |
| α-helix | 1087-1100 | 14 | |
| α-helix | 1128-1131 | 4 | |
| α-helix | 1136-1137 | 2 | |
| α-helix | 1139-1161 | 23 | |
| α-helix | 1168-1187 | 20 | |
| α-helix | 1191-1205 | 15 | |
| α-helix | 1210-1229 | 20 | |
| α-helix | 1239-1252 | 14 | |
| α-helix | 1253-1255 | 3 | |
| α-helix | 1261-1276 | 16 | |
| α-helix | 1284-1292 | 9 | |
| α-helix | 1293-1295 | 3 | |
| α-helix | 1300-1324 | 25 | |
| β-strand | 1388-1394 | 7 | 7 |
| α-helix | 1419-1432 | 14 | |
| β-strand | 1448-1456 | 9 | 7 |
| β-strand | 1457-1458 | 2 | 8 |
| β-strand | 1461-1462 | 2 | 8 |
| β-strand | 1464 | 1 | 9 |
| β-strand | 1465-1466 | 2 | 7 |
| α-helix | 1467-1477 | 11 | |
| β-strand | 1482-1483 | 2 | 10 |
| α-helix | 1484-1491 | 8 | |
| α-helix | 1498-1502 | 5 | |
| α-helix | 1504-1506 | 3 | |
| β-strand | 1513 | 1 | 11 |
| β-strand | 1524-1525 | 2 | 10 |
| β-strand | 1526 | 1 | 11 |
| β-strand | 1542 | 1 | 9 |
| α-helix | 1545-1547 | 3 | |
| α-helix | 1555-1576 | 22 | |
| β-strand | 1583-1584 | 2 | 12 |
| α-helix | 1585-1597 | 13 | |
| α-helix | 1604-1609 | 6 | |
| α-helix | 1618-1631 | 14 | |
| β-strand | 1634-1636 | 3 | 12 |
| β-strand | 1643-1646 | 4 | 12 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 122-129 | 8 | |
| α-helix | 133-136 | 4 | |
| α-helix | 139-141 | 3 | |
| α-helix | 144-150 | 7 | |
| α-helix | 153-163 | 11 | |
| α-helix | 177-196 | 20 | |
| β-strand | 201-204 | 4 | 2 |
| α-helix | 205-208 | 4 | |
| β-strand | 213-218 | 6 | 13 |
| β-strand | 222-227 | 6 | 13 |
| β-strand | 232-236 | 5 | 13 |
| β-strand | 239 | 1 | 14 |
| α-helix | 245 | 1 | |
| β-strand | 246 | 1 | 13 |
| α-helix | 247-248 | 2 | |
| α-helix | 260-261 | 2 | |
| β-strand | 262-267 | 6 | 15 |
| β-strand | 271-276 | 6 | 15 |
| β-strand | 280-285 | 6 | 15 |
| β-strand | 294-297 | 4 | 15 |
| β-strand | 304-307 | 4 | 16 |
| β-strand | 314-317 | 4 | 16 |
| β-strand | 321-326 | 6 | 16 |
| β-strand | 334-340 | 7 | 16 |
| β-strand | 347-350 | 4 | 17 |
| β-strand | 359-364 | 6 | 17 |
| β-strand | 367-372 | 6 | 17 |
| β-strand | 377-382 | 6 | 17 |
| α-helix | 387 | 1 | |
| β-strand | 388-393 | 6 | 18 |
| β-strand | 397-402 | 6 | 18 |
| β-strand | 405 | 1 | 19 |
| β-strand | 410 | 1 | 19 |
| β-strand | 413-418 | 6 | 18 |
| β-strand | 424-428 | 5 | 18 |
| β-strand | 435-439 | 5 | 20 |
| α-helix | 444 | 1 | |
| β-strand | 447-452 | 6 | 20 |
| β-strand | 456-461 | 6 | 20 |
| β-strand | 467-472 | 6 | 20 |
| β-strand | 478-483 | 6 | 21 |
| β-strand | 488-492 | 5 | 21 |
| β-strand | 496-501 | 6 | 21 |
| β-strand | 506-512 | 7 | 21 |
| β-strand | 517-523 | 7 | 2 |
| β-strand | 526-531 | 6 | 2 |
| β-strand | 555-560 | 6 | 2 |
| α-helix | 563-565 | 3 | |
| β-strand | 567 | 1 | 14 |
| α-helix | 580-592 | 13 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 24-32 | 9 | 7 |
| β-strand | 41 | 1 | 22 |
| β-strand | 48 | 1 | 22 |
| α-helix | 54-58 | 5 | |
| β-strand | 70-72 | 3 | 23 |
| β-strand | 73 | 1 | 24 |
| β-strand | 78-80 | 3 | 23 |
| α-helix | 81-90 | 10 | |
| β-strand | 103 | 1 | 24 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 4-7 | 4 | 25 |
| β-strand | 13-16 | 4 | 25 |
| α-helix | 18-21 | 4 | |
| α-helix | 25-32 | 8 | |
| β-strand | 45-46 | 2 | 25 |
| α-helix | 52-64 | 13 | |
| α-helix | 91-93 | 3 | |
| α-helix | 97-110 | 14 | |
| α-helix | 113-127 | 15 | |
| α-helix | 132-138 | 7 | |
| α-helix | 147-156 | 10 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| Cullin-7 | C | protein | 1700 | Homo sapiens | Q14999 (AlphaFold model) |
| F-box/WD repeat-containing protein 8 | F | protein | 600 | Homo sapiens | Q8N3Y1 (AlphaFold model) |
| E3 ubiquitin-protein ligase RBX1 | R | protein | 108 | Homo sapiens | P62877 (AlphaFold model) |
| S-phase kinase-associated protein 1 | S | protein | 163 | Homo sapiens | P63208 (AlphaFold model) |
>7Z8B_1 Cullin-7 (chains C) GPMVGELRYREFRVPLGPGLHAYPDELIRQRVGHDGHPEYQIRWLILRRGDEGDGGSGQV DCKAEHILLWMSKDEIYANCHKMLGEDGQVIGPSQESAGEVGALDKSVLEEMETDVKSLI QRALRQLEECVGTIPPAPLLHTVHVLSAYASIEPLTGVFKDPRVLDLLMHMLSSPDYQIR WSAGRMIQALSSHDAGTRTQILLSLSQQEAIEKHLDFDSRCALLALFAQATLSEHPMSFE GIQLPQVPGRVLFSLVKRYLHVTSLLDQLNDSAAEPGAQNTSAPEELSGERGQLELEFSM AMGTLISELVQAMRWDQASDRPRSSARSPGSIFQPQLADVSPGLPAAQAQPSFRRSRRFR PRSEFASGNTYALYVRDTLQPGMRVRMLDDYEEISAGDEGEFRQSNNGVPPVQVFWESTG RTYWVHWHMLEILGFEEDIEDMVEADEYQGAVASRVLGRALPAWRWRPMTELYAVPYVLP EDEDTEECEHLTLAEWWELLFFIKKLDGPDHQEVLQILQENLDGEILDDEILAELAVPIE LAQDLLLTLPQRLNDSALRDLINCHVYKKYGPEALAGNQAYPSLLEAQEDVLLLDAQAQA KDSEDAAKVEAKEPPSQSPNTPLQRLVEGYGPAGKILLDLEQALSSEGTQENKVKPLLLQ LQRQPQPFLALMQSLDTPETNRTLHLTVLRILKQLVDFPEALLLPWHEAVDACMACLRSP NTDREVLQELIFFLHRLTSVSRDYAVVLNQLGARDAISKALEKHLGKLELAQELRDMVFK CEKHAHLYRKLITNILGGCIQMVLGQIEDHRRTHRPINIPFFDVFLRYLCQGSSVEVKED KCWEKVEVSSNPHRASKLTDHNPKTYWESNGSAGSHYITLHMRRGILIRQLTLLVASEDS SYMPARVVVCGGDSTSSLHTELNSVNVMPSASRVILLENLTRFWPIIQIRIKRCQQGGID TRIRGLEILGPKPTFWPVFREQLCRHTRLFYMVRAQAWSQDMAEDRRSLLHLSSRLNGAL RQEQNFADRFLPDDEAAQALGKTCWEALVSPVVQNITSPDEDGISPLGWLLDQYLECQEA VFNPQSRGPAFFSRVRRLTHLLVHVEPCEAPPPVVATPRPKGRNRSHDWSSLATRGLPSS IMRNLTRCWRAVVEKQVNNFLTSSWRDDDFVPRYCEHFNILQNSSSELFGPRAAFLLALQ NGCAGALLKLPFLKAAHVSEQFARHIDQQIQGSRIGGAQEMERLAQLQQCLQAVLIFSGL EIATTFEHYYQHYMADRLLGVVSSWLEGAVLEQIGPCFPNRLPQQMLQSLSTSKELQRQF HVYQLQQLDQELLKLEDTEKKIQVGLGASGKEHKSEKEEEAGAAAVVDVAEGEEEEEENE DLYYEGAMPEVSVLVLSRHSWPVASICHTLNPRTCLPSYLRGTLNRYSNFYNKSQSHPAL ERGSQRRLQWTWLGWAELQFGNQTLHVSTVQMWLLLYLNDLKAVSVESLLAFSGLSADML NQAIGPLTSSRGPLDLHEQKDIPGGVLKIRDGSKEPRSRWDIVRLIPPQTYLQAEGEDGQ NLEKRRNLLNCLIVRILKAHGDEGLHIDQLVCLVLEAWQKGPCPPRGLVSSLGKGSACSS TDVLSCILHLLGKGTLRRHDDRPQVLSYAVPVTVMEPHTESLNPGSSGPNPPLTFHTLQI RSRGVPYASCTATQSFSTFR
>7Z8B_2 F-box/WD repeat-containing protein 8 (chains F) GPMDDYSLDEFRRRWQEELAQAQAPKKRRRPEAAERRARRPEVGSGRGEQASGDPALAQR LLEGAGRPPAARATRAEGQDVASRSRSPLAREGAGGGEQLVDQLIRDLNEMNDVPFFDIQ LPYELAINIFQYLDRKELGRCAQVSKTWKVIAEDEVLWYRLCQQEGHLPDSSISDYSCWK LIFQECRAKEHMLRTNWKNRKGAVSELEHVPDTVLCDVHSHDGVVIAGYTSGDVRVWDTR TWDYVAPFLESEDEEDEPGMQPNVSFVRINSSLAVAAYEDGFLNIWDLRTGKYPVHRFEH DARIQALALSQDDATVATASAFDVVMLSPNEEGYWQIAAEFEVPKLVQYLEIVPETRRYP VAVAAAGDLMYLLKAEDSARTLLYAHGPPVTCLDVSANQVAFGVQGLGWVYEGSKILVYS LEAGRRLLKLGNVLRDFTCVNLSDSPPNLMVSGNMDGRVRIHDLRSGNIALSLSAHQLRV SAVQMDDWKIVSGGEEGLVSVWDYRMNQKLWEVYSGHPVQHISFSSHSLITANVPYQTVM RNADLDSFTTHRRHRGLIRAYEFAVDQLAFQSPLPVCRSSCDAMATHYYDLALAFPYNHV
>7Z8B_3 E3 ubiquitin-protein ligase RBX1 (chains R) MAAAMDVDTPSGTNSGAGKKRFEVKKWNAVALWAWDIVVDNCAICRNHIMDLCIECQANQ ASATSEECTVAWGVCNHAFHFHCISRWLKTRQVCPLDNREWEFQKYGH
>7Z8B_4 S-phase kinase-associated protein 1 (chains S) MPSIKLQSSDGEIFEVDVEIAKQSVTIKTMLEDLGMDDEGDDDPVPLPNVNAAILKKVIQ WCTHHKDDPPPPEDDENKEKRTDDIPVWDQEFLKVDQGTLFELILAANYLDIKGLLDVTC KTVANMIKGKTPEEIRKTFNIKNDFTEEEEAQVRKENQWCEEK
| ID | Name | Formula | Copies |
|---|---|---|---|
| ZN | Zinc ion | Zn | 3 |
Structure of CRL7 FBXW8 reveals coupling with CUL1-RBX1/ROC1 for multi-cullin-RING E3-catalyzed ubiquitin ligation. Hopf, L.V.M., Baek, K., Klugel, M. et al. Nat Struct Mol Biol (2022) 29:854-862. DOI 10.1038/s41594-022-00815-6 · PubMed
Other PDB entries of the same protein (UniProt Q14999 (AlphaFold model), which also has an AlphaFold model), best resolution first:
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