8A5N: Human H-chain ferritin treated with SDS

X-ray structure of human H-chain ferritin treated with SDS. Determined by X-ray diffraction at 1.52 Å resolution. Released 8 Feb 2023.

Method: X-ray diffraction
Resolution: 1.52 Å
Organism: Homo sapiens
Chains: 1
Atoms: 1,920
Mol. weight: 21.87 kDa
Ligands: FE, MG
Released: 8 Feb 2023

Explore 8A5N in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8A5N contains 9 α-helices and 0 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain AAA: 9 helices, 0 β-strands

Element	Residues	Length
α-helix	4-6	3
α-helix	14-41	28
α-helix	49-76	28
α-helix	86-88	3
α-helix	96-123	28
α-helix	127-133	7
α-helix	134-138	5
α-helix	139-158	20
α-helix	164-173	10

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Ferritin heavy chain	AAA	protein	182	Homo sapiens	P02794 (AlphaFold model)

Sequence of entity 1 (AAA), FASTA

>8A5N_1 Ferritin heavy chain (chains AAA)
TTASTSQVRQNYHQDSEAAINRQINLELYASYVYLSMSYYFDRDDVALKNFAKYFLHQSH
EEREHAEKLMKLQNQRGGRIFLQDIKKPDCDDWESGLNAMECALHLEKNVNQSLLELHKL
ATDKNDPHLCDFIETHYLNEQVKAIKELGDHVTNLRKMGAPESGLAEYLFDKHTLGDSDN
ES

Ligands and cofactors

ID	Name	Formula	Copies
FE	FE (III) ion	Fe	1
MG	Magnesium ion	Mg	10

Water and common crystallization additives (GOL, CL) are not listed.

Primary citation

A new and efficient procedure to load bioactive molecules within the human heavy-chain ferritin nanocage. Lucignano, R., Stanzione, I., Ferraro, G. et al. Front Mol Biosci (2023) 10:1008985-1008985. DOI 10.3389/fmolb.2023.1008985 · PubMed

Other PDB entries of the same protein (UniProt P02794 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6B8F 1.06 Å, Contracted Human Heavy-Chain Ferritin Crystal-Hydrogel Hybrid
6B8G 1.13 Å, Twice-Contracted Human Heavy-Chain Ferritin Crystal-Hydrogel Hybrid
7A6A 1.15 Å, 1.15 A structure of human apoferritin obtained from Titan Mono- BCOR microscope
8B7O 1.17 Å, X-ray structure of Auranofin-human H-chain ferritin
6WYF 1.25 Å, Crystal structure of Human H-chain Ferritin variant 157C Delta C-star Modified with a…
6Z6U 1.25 Å, 1.25 A structure of human apoferritin obtained from Titan Mono-BCOR microscope
7RRP 1.27 Å, Apoferritin structure at 1.27 angstrom resolution determined from a 300 kV Titan Krios…
7A6B 1.33 Å, 1.33 A structure of human apoferritin obtained from Titan Mono- BCOR microscope
4Y08 1.34 Å, One minute iron loaded human H ferritin
7K3V 1.34 Å, Apoferritin structure at 1.34 angstrom resolution determined from a 300 kV Titan Krios…
7K3W 1.36 Å, Apoferritin structure at 1.36 angstrom resolution determined from a 300 kV Titan Krios…
9RGH 1.38 Å, X-ray structure of a polyoxidovanadate/human H-ferritin adduct obtained when the protein…

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