8D4O: Extracellular Adherence Protein

Crystal Structure of the Neutrophil Serine Protease Inhibitor Eap1 from S. aureus. Determined by X-ray diffraction at 1.45 Å resolution. Released 21 Dec 2022.

Method: X-ray diffraction
Resolution: 1.45 Å
Organism: Staphylococcus aureus subsp. aureus Mu50
Chains: 4
Atoms: 3,651
Mol. weight: 44.83 kDa
Released: 21 Dec 2022

Explore 8D4O in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8D4O contains 12 α-helices and 32 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chains A and C: 3 helices, 8 β-strands

Element	Residues	Length	Sheet
β-strand	48-52	5	1
β-strand	53-56	4	2
β-strand	67-71	5	1
β-strand	75-76	2	3
α-helix	78-93	16
α-helix	97-102	6
β-strand	106-112	7	2
β-strand	117-121	5	2
β-strand	132-133	2	3
α-helix	134-136	3
β-strand	137-144	8	2

Chains B and D: 3 helices, 8 β-strands

Element	Residues	Length	Sheet
β-strand	48-52	5	4
β-strand	53-56	4	2
β-strand	67-71	5	4
β-strand	75-76	2	5
α-helix	78-93	16
α-helix	97-101	5
β-strand	106-112	7	2
β-strand	117-121	5	2
β-strand	132-133	2	5
α-helix	134-136	3
β-strand	137-144	8	2

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Extracellular Adherence Protein	A, B, C, D	protein	100	Staphylococcus aureus subsp. aureus Mu50	Q99QS1 (AlphaFold model)

Sequence of entity 1 (A, B, C, D), FASTA

>8D4O_1 Extracellular Adherence Protein (chains A, B, C, D)
GSTIQIPYTITVNGTSQNILSSLTFNKNQNISYKDIENKVKSVLYFNRGISDIDLRLSKQ
AEYTVHFKNGTKRVIDLKSGIYTADLINTSDIKAISVNVD

Primary citation

Characterization of two distinct neutrophil serine protease-binding modes within a Staphylococcus aureus innate immune evasion protein family. Gido, C.D., Herdendorf, T.J., Geisbrecht, B.V. J Biol Chem (2023) 299:102969-102969. DOI 10.1016/j.jbc.2023.102969 · PubMed

Other PDB entries of the same protein (UniProt Q99QS1 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1YN3 1.35 Å, Crystal Structures of EAP Domains from Staphylococcus aureus Reveal an Unexpected…
9ASS 1.75 Å, Crystal Structure of Neutrophil Elastase Inhibited by Eap4 from S. aureus
8D4V 1.85 Å, Crystal Structure of Cathepsin G Inhibited by Eap2 from S. aureus
8D4U 1.9 Å, Crystal Structure of Neutrophil Elastase Inhibited by Eap2 from S. aureus
8D4S 1.95 Å, Crystal Structure of Cathepsin G Inhibited by Eap1 from S. aureus
9ASX 1.96 Å, BIFUNCTIONAL INHIBITION OF NEUTROPHIL ELASTASE AND CATHEPSIN G by Eap3 of S. aureus
9ATU 2.05 Å, Bifunctional Inhibition of Neutrophil Elastase by Eap4 from S. aureus
9ATK 2.11 Å, BIFUNCTIONAL INHIBITION OF NEUTROPHIL ELASTASE AND CATHEPSIN G by Eap4 of S. aureus
8D4Q 2.2 Å, Crystal Structure of Neutrophil Elastase Inhibited by Eap1 from S. aureus
8D7K 3.1 Å, Bifunctional Inhibition of Neutrophil Elastase and Cathepsin G by Eap2 from S. aureus
8D7I 3.63 Å, Bifunctional Inhibition of Neutrophil Elastase and Cathepsin G by Eap1 from S. aureus

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