Cryo-EM structure of human ferroportin/slc40 bound to minihepcidin PR73 in nanodisc. Determined by electron microscopy at 2.7 Å resolution. Released 7 Dec 2022.
Explore 8DL7 in 3D Show helices and sheets RCSB PDB PDBe
8DL7 contains 29 α-helices and 40 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 14-20 | 7 | |
| α-helix | 24-53 | 30 | |
| α-helix | 58-83 | 26 | |
| α-helix | 88-115 | 28 | |
| α-helix | 118-122 | 5 | |
| α-helix | 127-152 | 26 | |
| α-helix | 153-159 | 7 | |
| α-helix | 160-163 | 4 | |
| α-helix | 168-197 | 30 | |
| α-helix | 201-229 | 29 | |
| α-helix | 231-233 | 3 | |
| α-helix | 285-303 | 19 | |
| α-helix | 308-316 | 9 | |
| α-helix | 326-335 | 10 | |
| α-helix | 340-366 | 27 | |
| α-helix | 370-389 | 20 | |
| α-helix | 453-482 | 30 | |
| α-helix | 486-488 | 3 | |
| α-helix | 489-513 | 25 | |
| α-helix | 520-546 | 27 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 24-25 | 2 | 1 |
| β-strand | 30-32 | 3 | 2 |
| β-strand | 39-45 | 7 | 1 |
| β-strand | 50 | 1 | 3 |
| β-strand | 53-58 | 6 | 4 |
| β-strand | 65-69 | 5 | 4 |
| β-strand | 73-74 | 2 | 4 |
| β-strand | 82-87 | 6 | 1 |
| β-strand | 88 | 1 | 3 |
| β-strand | 90-95 | 6 | 1 |
| α-helix | 100-102 | 3 | |
| β-strand | 105-110 | 6 | 4 |
| α-helix | 116 | 1 | |
| β-strand | 117-118 | 2 | 4 |
| β-strand | 123-125 | 3 | 2 |
| β-strand | 137-138 | 2 | 5 |
| α-helix | 139-141 | 3 | |
| α-helix | 142-146 | 5 | |
| β-strand | 150-159 | 10 | 5 |
| β-strand | 165-170 | 6 | 6 |
| β-strand | 174 | 1 | 6 |
| β-strand | 179-183 | 5 | 5 |
| β-strand | 193-201 | 9 | 5 |
| α-helix | 203-207 | 5 | |
| β-strand | 212-218 | 7 | 6 |
| β-strand | 221-228 | 8 | 6 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 22-25 | 4 | 7 |
| β-strand | 29-31 | 3 | 8 |
| β-strand | 36-44 | 9 | 7 |
| α-helix | 48-50 | 3 | |
| β-strand | 52-58 | 7 | 8 |
| β-strand | 64-70 | 7 | 8 |
| β-strand | 77-79 | 3 | 8 |
| α-helix | 81-83 | 3 | |
| β-strand | 87-92 | 6 | 7 |
| β-strand | 97-103 | 7 | 7 |
| α-helix | 107-109 | 3 | |
| β-strand | 111-118 | 8 | 8 |
| β-strand | 133-137 | 5 | 8 |
| β-strand | 145-146 | 2 | 9 |
| β-strand | 149 | 1 | 9 |
| β-strand | 163-170 | 8 | 9 |
| β-strand | 176-179 | 4 | 10 |
| β-strand | 188-190 | 3 | 9 |
| α-helix | 191-193 | 3 | |
| β-strand | 194-196 | 3 | 9 |
| β-strand | 199-206 | 8 | 9 |
| β-strand | 218-224 | 7 | 10 |
| β-strand | 229-235 | 7 | 10 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| Solute carrier family 40 member 1 | A | protein | 577 | Homo sapiens | Q9NP59 (AlphaFold model) |
| 11F9 light-chain | C | protein | 213 | Mus musculus | |
| 11F9 heavy-chain | D | protein | 238 | Mus musculus | |
| Minihepcidin PR73 | F | protein | 12 | synthetic construct |
>8DL7_1 Solute carrier family 40 member 1 (chains A) MTRAGDHNRQRGCCGSLADYLTSAKFLLYLGHSLSTWGDRMWHFAVSVFLVELYGNSLLL TAVYGLVVAGSVLVLGAIIGDWVDKNARLKVAQTSLVVQNVSVILCGIILMMVFLHKHEL LTMYHGWVLTSCYILIITIANIANLASTATAITIQRDWIVVVAGEDRSKLANMNATIRRI DQLTNILAPMAVGQIMTFGSPVIGCGFISGWNLVSMCVEYVLLWKVYQKTPALAVKAGLK EEETELKQLNLHKDTEPKPLEGTHLMGVKDSNIHELEHEQEPTCASQMAEPFRTFRDGWV SYYNQPVFLAGMGLAFLYMTVLGFDCITTGYAYTQGLSGSILSILMGASAITGIMGTVAF TWLRRKCGLVRTGLISGLAQLSCLILCVISVFMPGSPLDLSVSPFEDIRSRFIQGESITP TKIPEITTEIYMSNGSNSANIVPETSPESVPIISVSLLFAGVIAARIGLWSFDLTVTQLL QENVIESERGIINGVQNSMNYLLDLLHFIMVILAPNPEAFGLLVLISVSFVAMGHIMYFR FAQNTLGNKLFACGPDAKEVRKENQANTSVVENLYFQ
>8DL7_2 11F9 light-chain (chains C) DIVMTQSQKFMSTSVGDRVSITCKASQNVGTAVAWYQKKPGQSPKLLIYSASNRYSGVPD RFTGSGSGTDFTLTISNMQSEDLADYFCQQYGSYPLTFGSGTKLEIKEAEAAPTVSIFPP SSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLT LTKDEYERHNSYTCEATHKTSTSPIVKSFNRNE
>8DL7_3 11F9 heavy-chain (chains D) MKCSWVIFFLMAVVTGVNSEVQLQQSGAELVRPGALVKLSCKASGFNIKDYYMHWVKERP EQGLEWIGWIDPENGNTIYDPKFQGKASITADTSSNTAYLQLSSLTSEDTAVYYCARKRG YYGPYFDYWGQGTTLTVSSKTTAPSVYPLAPVCGDTTGSSVTLGCLVKGYFPEPVTLTWN SGSLSSGVHTFPAVLQSGLYTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKIEPA
>8DL7_4 Minihepcidin PR73 (chains F) XTHXXRCRXXXX
| ID | Name | Formula | Copies |
|---|---|---|---|
| POV | (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl… | C42 H82 N O8 P | 2 |
Structural basis of ferroportin inhibition by minihepcidin PR73. Wilbon, A.S., Shen, J., Ruchala, P. et al. PLoS Biol (2023) 21:e3001936-e3001936. DOI 10.1371/journal.pbio.3001936 · PubMed
Other PDB entries of the same protein (UniProt Q9NP59 (AlphaFold model), which also has an AlphaFold model), best resolution first:
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