8EAS: Yeast VO

Yeast VO in complex with Vma12-22p. Determined by electron microscopy at 2.6 Å resolution. Released 2 Nov 2022.

Method
Electron microscopy
Resolution
2.6 Å
Organism
Saccharomyces cerevisiae
Chains
18
Atoms
24,802
Mol. weight
404.46 kDa
Released
2 Nov 2022

Explore 8EAS in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8EAS contains 148 α-helices and 36 β-strands across 18 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain a: 44 helices, 22 β-strands

ElementResiduesLengthSheet
β-strand1015
α-helix11-133
β-strand14-2296
α-helix23-253
α-helix26-3611
β-strand3917
β-strand40-4126
α-helix56-7823
α-helix105-14541
α-helix150-1523
β-strand186-19278
α-helix193-1953
α-helix196-20611
β-strand213-21758
β-strand222-22439
β-strand229-23139
β-strand233-23978
α-helix243-25513
β-strand259-26138
α-helix268-2703
α-helix271-32454
β-strand327-32936
β-strand334-34296
α-helix343-3453
α-helix346-35914
β-strand368-37036
α-helix376-3783
β-strand379110
α-helix388-39710
α-helix399-4013
β-strand40515
α-helix4061
α-helix408-42215
α-helix426-44116
α-helix443-4464
α-helix454-4607
α-helix462-47918
β-strand482111
β-strand485111
β-strand494-495212
β-strand506-507213
β-strand509-510212
α-helix520-5223
α-helix528-56134
α-helix565-5673
α-helix568-5725
α-helix573-5797
α-helix580-5845
α-helix585-59511
α-helix598-6014
α-helix603-6075
α-helix608-61710
α-helix630-65728
α-helix710-73223
α-helix734-75017
α-helix751-7555
α-helix756-7583
α-helix763-78018
α-helix781-7899
α-helix790-79910
α-helix800-8056
α-helix806-8094
α-helix814-8152
β-strand816110
β-strand82017
α-helix827-8326
Chain A: 4 helices, 2 β-strands
ElementResiduesLengthSheet
α-helix16-4934
α-helix62-643
β-strand74-8071
β-strand83-8971
α-helix132-1365
α-helix144-17633
Chain b: 2 helices, 0 β-strands
ElementResiduesLengthSheet
α-helix221-24525
α-helix256-2572
Chain B: 6 helices, 3 β-strands
ElementResiduesLengthSheet
β-strand2-542
α-helix8-1710
α-helix25-3713
β-strand39-4022
α-helix42-5211
β-strand70-7342
α-helix74-763
α-helix85-10622
α-helix125-15026
Chain c: 11 helices, 0 β-strands
ElementResiduesLengthSheet
α-helix17-3822
α-helix42-443
α-helix47-537
α-helix56-8934
α-helix96-12227
α-helix126-1294
α-helix130-1323
α-helix136-17641
α-helix178-1803
α-helix181-20525
α-helix210-2123
Chain d: 17 helices, 0 β-strands
ElementResiduesLengthSheet
α-helix2-2221
α-helix27-348
α-helix39-468
α-helix65-8521
α-helix89-11527
α-helix120-1256
α-helix137-1404
α-helix145-1484
α-helix149-1535
α-helix159-1624
α-helix174-19926
α-helix202-22726
α-helix235-2395
α-helix251-2566
α-helix262-2698
α-helix284-29916
α-helix307-33125
Chain e: 2 helices, 2 β-strands
ElementResiduesLengthSheet
α-helix4-2421
α-helix30-5425
β-strand63-64213
β-strand67114
Chain f: 3 helices, 1 β-strand
ElementResiduesLengthSheet
α-helix8-3225
α-helix36-394
β-strand41114
α-helix49-7527

9 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Vacuolar ATPase assembly protein VMA22Aprotein181Saccharomyces cerevisiaeP38784 (AlphaFold model)
V-type proton ATPase assembly factor Vma12pBprotein147Saccharomyces cerevisiaeP32341 (AlphaFold model)
V-type proton ATPase subunit FFprotein118Saccharomyces cerevisiaeP39111 (AlphaFold model)
V-type proton ATPase subunit a, vacuolar isoformaprotein840Saccharomyces cerevisiaeP32563 (AlphaFold model)
V0 assembly protein 1bprotein265Saccharomyces cerevisiaeP53262
V-type proton ATPase subunit c''cprotein213Saccharomyces cerevisiaeP23968
V-type proton ATPase subunit ddprotein345Saccharomyces cerevisiaeP32366
V-type proton ATPase subunit eeprotein73Saccharomyces cerevisiaeQ3E7B6
Yeast V-ATPase subunit ffprotein85Saccharomyces cerevisiaeP0C5R9
V-type proton ATPase subunit cg, h, i, j, k, l, m, nprotein160Saccharomyces cerevisiaeP25515
V-type proton ATPase subunit c'oprotein164Saccharomyces cerevisiaeP32842
Sequence of entity 1 (A), FASTA
>8EAS_1 Vacuolar ATPase assembly protein VMA22 (chains A)
MSETRMAQNMDTTDEQYLRLIELLSNYDSTLEQLQKGFQDGYIQLSRSNYYNKDSLRGNY
GEDYWDETYIGQLMATVEEKNSKVVVEIVKRKAQDKQEKKEEEDNKLTQRKKGTKPEKQK
TQSHKLKQDYDPILMFGGVLSVPSSLRQSQTSFKGCIPLIAQLINYKNEILTLVETLSEQ
E
Sequence of entity 2 (B), FASTA
>8EAS_2 V-type proton ATPase assembly factor Vma12p (chains B)
MFEIKLNDRITEFLRKFKNSAKSNEGIDEDIDLFLKRHAIPMQSLLFYVKEYRKDSDLQC
SIKELLKPLEFEFKPKAVRGLHYSEDFKKKLEFLKYQEQELEYQSMVKXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXX
Sequence of entity 3 (F), FASTA
>8EAS_3 V-type proton ATPase subunit F (chains F)
MAEKRTLIAVIADEDTTTGLLLAGIGQITPETQEKNFFVYQEGKTTKEEITDKFNHFTEE
RDDIAILLINQHIAENIRARVDSFTNAFPAILEIPSKDHPYDPEKDSVLKRVRKLFGE
Sequence of entity 4 (a), FASTA
>8EAS_4 V-type proton ATPase subunit a, vacuolar isoform (chains a)
MAEKEEAIFRSAEMALVQFYIPQEISRDSAYTLGQLGLVQFRDLNSKVRAFQRTFVNEIR
RLDNVERQYRYFYSLLKKHDIKLYEGDTDKYLDGSGELYVPPSGSVIDDYVRNASYLEER
LIQMEDATDQIEVQKNDLEQYRFILQSGDEFFLKGDNTDSTSYMDEDMIDANGENIAAAI
GASVNYVTGVIARDKVATLEQILWRVLRGNLFFKTVEIEQPVYDVKTREYKHKNAFIVFS
HGDLIIKRIRKIAESLDANLYDVDSSNEGRSQQLAKVNKNLSDLYTVLKTTSTTLESELY
AIAKELDSWFQDVTREKAIFEILNKSNYDTNRKILIAEGWIPRDELATLQARLGEMIARL
GIDVPSIIQVLDTNHTPPTFHRTNKFTAGFQSICDCYGIAQYREINAGLPTIVTFPFMFA
IMFGDMGHGFLMTLAALSLVLNEKKINKMKRGEIFDMAFTGRYIILLMGVFSMYTGFLYN
DIFSKTMTIFKSGWKWPDHWKKGESITATSVGTYPIGLDWAWHGTENALLFSNSYKMKLS
ILMGFIHMTYSYFFSLANHLYFNSMIDIIGNFIPGLLFMQGIFGYLSVCIVYKWAVDWVK
DGKPAPGLLNMLINMFLSPGTIDDELYPHQAKVQVFLLLMALVCIPWLLLVKPLHFKFTH
KKKSHEPLPSTEADASSEDLEAQQLISAMDADDAEEEEVGSGSHGEDFGDIMIHQVIHTI
EFCLNCVSHTASYLRLWALSLAHAQLSSVLWTMTIQIAFGFRGFVGVFMTVALFAMWFAL
TCAVLVLMEGTSAMLHSLRLHWVESMSKFFVGEGLPYEPFAFEYKDMEVAVASASSSASS
Sequence of entity 5 (b), FASTA
>8EAS_5 V0 assembly protein 1 (chains b)
MVFGQLYALFIFTLSCCISKTVQADSSKESSSFISFDKESNWDTISTISSTADVISSVDS
AIAVFEFDNFSLLDNLMIDEEYPFFNRFFANDVSLTVHDDSPLNISQSLSPIMEQFTVDE
LPESASDLLYEYSLDDKSIVLFKFTSDAYDLKKLDEFIDSCLSFLEDKSGDNLTVVINSL
GWAFEDEDGDDEYATEETLSHHDNNKGKEGDDDILSSIWTEGLLMCLIVSALLLFILIVA
LSWISNLDITYGALEKSTNPIKKNN
Sequence of entity 6 (c), FASTA
>8EAS_6 V-type proton ATPase subunit c'' (chains c)
MNKESKDDDMSLGKFSFSHFLYYLVLIVVIVYGLYKLFTGHGSDINFGKFLLRTSPYMWA
NLGIALCVGLSVVGAAWGIFITGSSMIGAGVRAPRITTKNLISIIFCEVVAIYGLIIAIV
FSSKLTVATAENMYSKSNLYTGYSLFWAGITVGASNLICGIAVGITGATAAISDAADSAL
FVKILVIEIFGSILGLLGLIVGLLMAGKASEFQ
Sequence of entity 7 (d), FASTA
>8EAS_7 V-type proton ATPase subunit d (chains d)
MEGVYFNIDNGFIEGVVRGYRNGLLSNNQYINLTQCDTLEDLKLQLSSTDYGNFLSSVSS
ESLTTSLIQEYASSKLYHEFNYIRDQSSGSTRKFMDYITYGYMIDNVALMITGTIHDRDK
GEILQRCHPLGWFDTLPTLSVATDLESLYETVLVDTPLAPYFKNCFDTAEELDDMNIEII
RNKLYKAYLEDFYNFVTEEIPEPAKECMQTLLGFEADRRSINIALNSLQSSDIDPDLKSD
LLPNIGKLYPLATFHLAQAQDFEGVRAALANVYEYRGFLETGNLEDHFYQLEMELCRDAF
TQQFAISTVWAWMKSKEQEVRNITWIAECIAQNQRERINNYISVY
Sequence of entity 8 (e), FASTA
>8EAS_8 V-type proton ATPase subunit e (chains e)
MSSFYTVVGVFIVVSAMSVLFWIMAPKNNQAVWRSTVILTLAMMFLMWAITFLCQLHPLV
APRRSDLRPEFAE
Sequence of entity 9 (f), FASTA
>8EAS_9 Yeast V-ATPase subunit f (chains f)
MRPVVSTGKAWCCTVLSAFGVVILSVIAHLFNTNHESFVGSINDPEDGPAVAHTVYLAAL
VYLVFFVFCGFQVYLARRKPSIELR
Sequence of entity 10 (g, h, i, j, k, l, m, n), FASTA
>8EAS_10 V-type proton ATPase subunit c (chains g, h, i, j, k, l, m, n)
MTELCPVYAPFFGAIGCASAIIFTSLGAAYGTAKSGVGICATCVLRPDLLFKNIVPVIMA
GIIAIYGLVVSVLVCYSLGQKQALYTGFIQLGAGLSVGLSGLAAGFAIGIVGDAGVRGSS
QQPRLFVGMILILIFAEVLGLYGLIVALLLNSRATQDVVC
Sequence of entity 11 (o), FASTA
>8EAS_11 V-type proton ATPase subunit c' (chains o)
MSTQLASNIYAPLYAPFFGFAGCAAAMVLSCLGAAIGTAKSGIGIAGIGTFKPELIMKSL
IPVVMSGILAIYGLVVAVLIAGNLSPTEDYTLFNGFMHLSCGLCVGFACLSSGYAIGMVG
DVGVRKYMHQPRLFVGIVLILIFSEVLGLYGMIVALILNTRGSE

Primary citation

Structural basis of V-ATPase V O region assembly by Vma12p, 21p, and 22p. Wang, H., Bueler, S.A., Rubinstein, J.L. Proc Natl Acad Sci U S A (2023) 120:e2217181120-e2217181120. DOI 10.1073/pnas.2217181120 · PubMed

Other PDB entries of the same protein (UniProt P38784 (AlphaFold model), which also has an AlphaFold model), best resolution first:

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